Application of circ-VAPA as gastric cancer and colorectal cancer diagnosis biomarker and treatment target point

A biomarker, colorectal cancer technology, applied in the application field of circ-VAPA as a diagnostic biomarker and therapeutic target for gastric and colorectal cancer

Inactive Publication Date: 2019-04-05
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology

However, the specific mechanism of action of most circRNAs in cancer has not yet been elucidated, so in-depth research on the mechanism of action of cir

Method used

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  • Application of circ-VAPA as gastric cancer and colorectal cancer diagnosis biomarker and treatment target point
  • Application of circ-VAPA as gastric cancer and colorectal cancer diagnosis biomarker and treatment target point
  • Application of circ-VAPA as gastric cancer and colorectal cancer diagnosis biomarker and treatment target point

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Experimental program
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Embodiment

[0017] 1. Experimental materials and methods:

[0018] Clinical samples: Tumor tissues and adjacent normal mucosal tissues were collected from 30 patients with gastric cancer and 60 patients with colorectal cancer who received surgical treatment in Beijing Chaoyang Hospital Affiliated to Capital Medical University during 2016-2018.

[0019] Cell lines and cell culture: human gastric cancer cell line HGC-27 and colorectal cancer cell line SW480 were purchased from American Type Culture Collection (ATCC). Cells were cultured in DMEM (Invitrogen, Carlsbad, CA, USA) medium containing 10% fetal bovine serum (Gibco, NY, USA), supplemented with 100 U / ml penicillin and 100 μg / ml streptomycin (Gibco, NY, USA) . at 37°C with 5% CO 2 grow cells in an environment.

[0020] RNA extraction and real-time fluorescent quantitative PCR (qRT-PCR): use Trizol (Invitrogen, Carlsbad, CA, USA) to extract the total RNA of cells and tissues: the reverse transcription kit uses PrimeScript TM RT re...

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Abstract

The invention discloses application of annular RNA VAPA (circ-VAPA) as a novel potential gastric cancer and colorectal cancer diagnosis biomarker and treatment target point. The circ-VAPA (hsa_circ_0006990|chr18:9931806-9937063+|VAPA) is formed by 2nd to 4th exons of a VAPA gene through reverse shearing and connection; the cyclization sequence has 338 basic groups. A qRT-PCR primer of the circ-VAPA and small interference RNA (siRNA) of in vitro interference circ-VAPA are prepared. Compared with normal tissues, the expression of the circ-VAPA in tumor tissues of patients with gastric cancer andcolorectal cancer is obviously up-regulated. In vitro experiments show that the circ-VAPA plays the role of the cancer promoting gene in the gastric cancer and colorectal cancer cells. The study results of the invention show that the circ-VAPA is a novel potential gastric cancer and colorectal cancer diagnosis biomarker and treatment target point.

Description

technical field [0001] The present invention relates to the application of tumor markers or potential therapeutic targets in the field of medical technology, in particular to the application of circ-VAPA as a diagnostic biomarker and therapeutic target for gastric cancer and colorectal cancer. Background technique [0002] More than 60% of malignant tumors are digestive system tumors, among which gastric cancer and colorectal cancer are the most common digestive system tumors. According to the latest global cancer statistics report in 2018, among all malignant tumors, the death rate of gastric cancer ranks third in the world, and the incidence rate ranks fifth in the world; the mortality rate of colorectal cancer ranks second in the world, and the incidence rate ranks first in the world three. Current studies have shown that the molecular pathogenesis of gastric cancer includes gene mutations (P53, ARID1A, FAT4, CDH1), abnormal signaling pathways related to tumorigenesis (W...

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 李向南王振军
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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