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Lactobacillus plantarum and its use for lowering uric acid, improving allergy and lowering blood sugar

A Lactobacillus plantarum, uric acid-lowering technology, applied in the direction of Lactobacillus, allergic diseases, bacteria, etc., can solve the problems that cannot meet the needs of relief

Active Publication Date: 2022-04-29
GRAPE KING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, strains with a single effect cannot meet the relief needs of various symptoms caused by obesity, and there is still a need to develop strains with compound effects in the field of medicine for prevention and / or treatment, so as to more effectively target obesity-related symptoms at the same time. The disease achieved the effect of improvement

Method used

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  • Lactobacillus plantarum and its use for lowering uric acid, improving allergy and lowering blood sugar
  • Lactobacillus plantarum and its use for lowering uric acid, improving allergy and lowering blood sugar
  • Lactobacillus plantarum and its use for lowering uric acid, improving allergy and lowering blood sugar

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Embodiment one: collection and separation and purification of strains

[0090] Visit farmers in Yilan, Hualien, Taitung and other places engaged in farming, and observe senior farmers over 75 years old who are still self-sufficient and flexible in farming. It is inferred that long-term intake of foods containing lactic acid bacteria, that is, long-term exposure to strains with unique beneficial effects, will help elderly farmers maintain good physiological functions. Therefore, samples that may contain lactic acid bacteria are collected from various places, and the samples are stored in sealed sterile bags, refrigerated in a mobile refrigerator, and brought back to the laboratory to isolate and purify the probiotic strains contained in the samples. First, prepare a medium containing different nutrient sources to screen out different types of lactic acid bacteria strains in the sample. Examples of the components and types of the medium are: MRS agar, PCA agar, M17 agar, ...

Embodiment 2

[0092] Embodiment two: the genetic analysis and identification of strain

[0093] Firstly, the DNA of GKM3 was collected for sequencing, and the strain was identified by gene comparison. Streak activation of GKM3, use the inoculation loop to pick up a single colony and inoculate it into the medium for amplification. Collect fresh bacteria the next day and extract strain gDNA by CTAB method, and confirm the quality of gDNA by 0.6% agar gel electrophoresis. After concentration, the third-generation sequencing technology platform PacBio RSII was used to analyze the whole genome of the strain. According to the analysis results of the whole genome, the whole genome of GKM3 is 3,076,381 bp in length and contains a 49,415 bp plasmid (SEQ ID NO: 1). The whole genome sequence of GKM3 was compared with the whole genome database, and the pheS (SEQ ID NO: 2) and recN (SEQ ID NO: 3) gene sequences of GKM3 were obtained. Next, the sequence of the pheS gene (SEQ ID NO: 2) of GKM3 was comp...

Embodiment 3

[0100] Embodiment three: strain culture

[0101] The effects of different culture conditions on the production of GKM3 were studied. The carbon source and nitrogen source in the fermentation medium for cultivating GKM3 were tested with a one-factor test method at a time.

[0102] First, starting from the demand for carbon sources, compare the number of colonies of GKM3 and the IFN-γ of peripheral leukocytes and lymphocytes under the conditions of 8 carbon sources: glucose, sucrose, lactose, fructose, mannose, sorbitol, glycerol and molasses Inducible ability to select a carbon source with high yield and high physiological activity of GKM3. In the MRS medium, 3 wt% of the above 8 carbon sources were added relative to the total weight of the medium, and the culture temperature was set at about 37° C. and the rotation speed was about 20 rpm. The preferred 4 carbon sources and the number of colonies formed by viable bacteria in the experimental results are shown in Table 2 below...

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Abstract

The present invention provides a Lactobacillus plantarum and its use for reducing uric acid, improving allergies and lowering blood sugar, specifically a Lactobacillus plantarum GKM3 with a specific gene sequence, which has acid resistance, alkali resistance and / or cell adsorption ability, and can reach Eliminate body fat, reduce blood lipids, reduce hepatomegaly, reduce liver function index, anti-inflammation, reduce uric acid, improve the effect of allergies and / or lower blood sugar. Accordingly, the present invention provides a new type of Lactobacillus plantarum, and further develops the medical application of the Lactobacillus plantarum as an alternative choice for preventing and / or treating obesity-related diseases in the field.

Description

technical field [0001] The present invention relates to a kind of Lactobacillus plantarum, a composition containing it, a culture method and its use; more specifically, it relates to a kind of Lactobacillus plantarum strain comprising a pheS gene, a recN gene and a plasmid. Eliminate body fat, reduce blood fat, reduce hepatomegaly, reduce liver function index, anti-inflammation, reduce uric acid, improve allergies and / or lower blood sugar, so as to prevent and / or treat obesity-related diseases. Background technique [0002] obesity [0003] With the change of life style, especially the change of eating habits, the rate of obesity has increased, making obesity a serious health problem in modern countries. The World Health Organization (WHO) has listed obesity as the most important issue of public health and preventive medicine in the 21st century. According to WHO's definition of obesity, BMI (Body mass index) is used as the standard, and BMI is greater than 30kg / m 2 Obesi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61P19/06A61P37/08A61P11/00A61P3/10A61P3/06A61P1/16C12R1/25
CPCA23L33/135C12N1/20A61K2035/115A23V2200/00C12R2001/25C12N1/205A23V2400/169A23V2200/304A23V2200/314A23V2200/328A23V2200/30A23V2200/3324
Inventor 陈劲初陈炎炼许胜杰林珊林诗伟李丽雅吴思颖陈彦博王启宪侯毓欣石仰慈林静雯陈雅君江佳琳
Owner GRAPE KING
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