A method for improving the activity of nuclease p1 produced by Penicillium citrinum
A technology for producing and nuclease from Penicillium citrinum, which is applied in the field of microbial fermentation, can solve the problems of long time consumption and low enzyme activity, achieve obvious effects, improve fermentation enzyme activity, and add less amount
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Embodiment 1
[0021] Preparation of fermentation medium: glucose 20g / L, peptone 2g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, plant Calcium Magnesium 0.2g / L, Zinc Phytate 0.1g / L, Tween-80 0.5g / L, Span-80 0.5g / L, RNA 0.5g / L, pH 6, sterilized at 121°C for 15min, cooled to 30°C ℃ for later use.
[0022] Semi-continuous fermentation: Put 6L culture medium in a 10L stirring tank, insert the Penicillium citrinum strain cultured on the wort slant medium after sterilization, and ferment for 56 hours at a temperature of 30°C in the fermenter The enzyme activity was stable at about 7300U / mL, and the volume of the fermented liquid released was 4.8L. The fermented liquid retained in the fermenter was used as the next batch of fermented seeds, and 4.8L of fermentation medium was prepared and put into the fermenter for further cultivation. After 20 hours, the enzyme activity stabilized to 7300U / mL, release 4.8L of ...
Embodiment 2
[0027] Preparation of fermentation medium: glucose 20g / L, peptone 2g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, plant Calcium Magnesium Oxide 0.2g / L, Zinc Phytate 0.1g / L, Tween-80 0.5g / L, Span-80 0.5g / L, RNA 0.5g / L, pH 6, sterilized at 121°C for 15min, cooled to 30°C ℃ for later use.
[0028] Semi-continuous fermentation: Put 60L culture medium in a 100L stirring tank, insert the Penicillium citrinum cultured on the wort slant culture medium after sterilization, and ferment for 56 hours at a temperature of 30°C in the fermentation tank The enzyme activity is stable at about 7400U / mL, and the volume of the fermented liquid released is 48L. The fermented liquid retained in the fermenter is used as the next batch of fermented seeds, and 48L of fermentation medium is prepared and put into the fermenter for further cultivation. After 20 hours, the enzyme activity was stabilized to 7480U / mL, an...
Embodiment 3
[0033] Preparation of fermentation medium: glucose 20g / L, peptone 2g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, magnesium sulfate 0.2g / L, calcium chloride 0.2g / L, plant Calcium Magnesium 0.2g / L, Zinc Phytate 0.1g / L, Tween-80 0.5g / L, Span-80 0.5g / L, RNA 0.5g / L, pH 6, sterilized at 121°C for 15min, cooled to 30°C ℃ for later use.
[0034]Semi-continuous fermentation: Put 600L medium in a 1000L stirring tank, insert the Penicillium citrinum cultured on the wort slant medium after sterilization, and ferment for 56 hours at a temperature of 30°C in the fermenter The enzyme activity is stable at about 7500U / mL, and the volume of the fermented liquid released is 480L. The fermented liquid retained in the fermenter is used as the next batch of fermented seeds, and 480L of fermentation medium is prepared and put into the fermenter for further cultivation. After 20 hours, the enzyme activity was stabilized to 7500U / mL, and 480L of fermentation brot...
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