grh gene related to epidermis development of German cockroach, dsrna of the gene, preparation method and application thereof

Abstract

The invention discloses a Grh gene relevant with blattella germanica crust development, dsRNA of the gene and a preparation method and application of the dsRNA. The nucleotide sequence of the Grh geneis shown as SEQID No.1, and the dsRNA is double stranded RNA consisting of the nucleotide sequence as shown in SEQID No.2 and the nucleotide sequence which is backward complementary with the nucleotide sequence as shown in SEQID No.2. An RNAi jamming technique is adopted, and through dsRNA synthesis, crust developmental relevant gene Grh is targetedly silent to influence exuviate of roaches, andbased on blocking or destroying roach exuviate, the purpose of roach control is achieved. A new molecular target is provided for pest control.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a Grh gene related to epidermis development of Blattella germanica, dsRNA of the gene, a preparation method and application thereof. Background technique [0002] Cockroaches are one of the most widely distributed and most difficult worldwide household hygiene pests. Cockroaches prefer humid, warm and dark living places, and are often nocturnal, so they are difficult to eliminate. Although cockroaches are small in size, they are extremely lethal. They not only bite clothes, contaminate food, but also spread diseases. They are carriers of various bacteria. Cockroaches can secrete some strange substances. Make their living places permeated with a special smell. At the same time, these secretions can make food taste bad and go bad, and bring certain harm to human life and agricultural production. [0003] Since the 1940s, chemical pesticides and organic insecticides ...

AI Technical Summary

Problems solved by technology

Cockroaches prefer humid, warm and dark living places, and are often nocturnal, so it is difficult to eliminate

Although cockroaches are small in size, they are extremely lethal. They not only bite clothes, contaminate food, but also spread diseases. They are carriers of various bacteria. Cockroaches can secrete some odorous substances. Make their living places permeated with a special smell. At the same time, these secretions can make food taste bad and deteriorate, and bring certain harm to human life and agricultural production.

Images