4 results about "Blatella germanica" patented technology

The application relates to a primer probe composition, a kit and a detection method for specifically detecting Blattella germanica. The specific primer and TaqMan probe are designed and screened based on the specific sequence of the mitochondrial COI gene of Blattella germanica. The detection method has extremely high specificity, has no cross reaction with close species such as Periplaneta americana and Blattella germanica, has high sensitivity, the minimum detection limit is 1 fg / muL, and has good repeatability, with the variation coefficients of groups and between groups being less than 3%.

The utility model relates to the technical field of cockroach prevention and control, in particular to a double-bait box for cockroach prevention and control, which comprises an upper cover and a lower shell which are detachably connected, and the lower shell comprises a connecting plate, and is characterized in that a first bait box and a second bait box are fixed at the top of the connecting plate; the first bait box is arranged on the left side of the top of the connecting plate, and the second bait box is arranged on the right side of the top of the connecting plate. Two bait boxes different in size are arranged on the connecting plate at the same time, bait for German cockroaches can be placed in the first bait box, bait for American cockroaches can be placed in the second bait box, the German cockroaches and the American cockroaches are prevented and controlled at the same time, and the effect of preventing and controlling the two cockroaches at the same time is improved; by arranging a first groove, a second groove and a clamping strip, the stability of inserting connection of the bait box and the upper cover can be improved; by arranging the mistaken eating prevention assembly, children can be prevented from eating mistakenly, and the safety of the device is improved.

The invention relates to a primer probe composition, a kit and a detection method for detecting German cockroach based on RPA (recombinase polymerase amplification), the composition comprises a specific primer pair and a probe, the upstream primer sequence of the specific primer pair is as shown in SEQ ID NO.1, and the downstream primer sequence of the specific primer pair is as shown in SEQ ID NO.2; and the sequence of the probe is as shown in SEQ ID NO.3. The method disclosed by the invention is simple and convenient to operate, high in sensitivity and strong in specificity, realizes visible results in a short time, does not need a precise instrument, and is suitable for rapidly detecting the German sickle.

The application belongs to but is not limited to the technical field of Blattella germanica prevention and treatment, and discloses a Blattella germanica prevention and treatment method and system based on nanoparticle delivery of lethal gene dsRNA, which comprises the following steps: Blattella germanica lethal gene full-length cloning; plasmid construction and dsRNA expression and purification; and RNAi feeding. The application first confirms an efficient RNAi lethal target point for Blattella germanica, ensures the efficiency of the technical scheme, realizes low-cost and large-scale preparation of an active ingredient of an RNAi insecticide, breaks through the cost bottleneck of commercial application, creatively constructs a nanocomposite delivery system, efficiently overcomes the technical bottleneck of oral delivery of RNAi, and provides an environment-friendly practical prevention and treatment scheme, and has important application value and prospect.