A kind of cryopreservation method of the eggs of A.

A technology for cryopreservation of A. chrysalis and cryopreservation, which is applied in the field of cryopreservation of insect eggs. It can solve problems such as the inability to determine the subsequent development stage, population and even the impact of the next generation of A. chrysalis, and achieve the goal of prolonging the development time. Effect

Active Publication Date: 2021-10-29
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that after the existing A. chinensis eggs are cryopreserved, it is impossible to determine the impact on the subsequent development stage, population and even the next generation of A. basilicum, the present invention provides a Low-temperature preservation method to achieve the longest preservation time for the eggs of A.

Method used

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  • A kind of cryopreservation method of the eggs of A.
  • A kind of cryopreservation method of the eggs of A.
  • A kind of cryopreservation method of the eggs of A.

Examples

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Embodiment 1

[0018] The cryopreservation method of the eggs of A. lotus flea beetle of the present embodiment comprises the following steps: taking the ovum laid on the day of A. lotus flea beetle on A. chinensis, and picking the leaves, and placing them in a petri dish. Moisturize the bottom with distilled water-wet filter paper, seal the top with plastic wrap, and tie small holes for ventilation. At a storage temperature of 15°C, preserve the eggs of A. chinensis. Fecundity, population parameters, and F 1 The hatching rate of the surrogate egg and the preservation time of the egg duration are 5 days.

Embodiment 2

[0020] The cryopreservation method of the eggs of A. lotus flea beetle of the present embodiment comprises the following steps: taking the ovum laid on the day of A. lotus flea beetle on A. chinensis, and picking the leaves, and placing them in a petri dish. Moisturize the bottom with 1% (m / v) agar medium, seal the top with plastic wrap, and pierce small holes for ventilation. At a storage temperature of 15°C, preserve A. chinensis eggs until the survival of each stage is not affected. rate, developmental time, lifespan, fecundity, population parameters, and F 1 The hatching rate of the surrogate egg and the preservation time of the egg duration are 7 days.

[0021] In order to verify the key influencing factors in the present invention, the present invention provides the following experimental process and results.

[0022] 【experiment material】

[0023] The insects tested were A. chinensis (originally collected from South China Agricultural University) kept in the insect cu...

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Abstract

The invention discloses a low-temperature preservation method for the eggs of A. chinensis A. chinensis, which comprises the following steps: taking the eggs laid on A. chinensis on the same day of A. chinensis, picking the leaves together, and placing them in a petri dish Use distilled water wet filter paper or 1% (m / v) agar medium at the bottom to keep the middle and bottom moist, seal the top with plastic wrap, and tie small holes for ventilation, and store A. The storage time of eggs is 5-7 days until the survival rate of each stage, adult fecundity and population growth are not affected. The invention solves the problem that the impact on the subsequent developmental stage, population and even the next generation of the A. chinensis can not be determined after the low-temperature preservation of the existing A. chinensis eggs. The invention can achieve the longest storage time for the eggs of the phalaenopsis beetle without affecting the subsequent development stage, population and even the next generation.

Description

technical field [0001] The invention relates to a method for low-temperature preservation of insect eggs, in particular to a method for low-temperature preservation of eggs of A. chinensis. Background technique [0002] With the rapid development of the global integration process, invasive organisms have increased significantly, threatening global ecological security and biological security. China is one of the countries most severely affected by alien biological invasions. Among the more than 600 identified invasive alien species, invasive plants account for nearly half. This has become one of the important factors restricting the economic development of our country. Alternanthera philoxeroides is a global malignant weed. It was introduced into my country in the 1930s and has now spread to more than 20 provinces, municipalities, and autonomous regions across the country. Ecological and other serious threats, resulting in annual economic losses of up to 600 million yuan. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 刘艳红王苑馨王锦华马瑞燕贾栋胡军王欣晨
Owner SHANXI AGRI UNIV
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