Low-temperature preservation method of agasicles hygrophila eggs

Abstract

The invention discloses a low-temperature preservation method of agasicles hygrophila eggs. The method comprises the following steps: taking eggs laid on alternanthera philoxeroides on the same day byagasicles hygrophila; picking the eggs with a leaf, and placing in a culture dish, moisturizing the bottom by using distilled water wet filter paper or a 1% (m / v) agar culture medium, sealing the topby using a preservative film, pricking small holes for ventilation, and preserving the agasicles hygrophila eggs at the preservation temperature of 15 DEG C for 5-7 days until the survival rate of each stage, the reproductive capacity of adults and population growth are not influenced. According to the method, the problem that the influence on the subsequent development stage, population and evennext generation of the agasicles hygrophila cannot be determined after the agasicles hygrophila eggs are preserved at low temperature in the prior art is solved. According to the method, the longestpreservation time of the agasicles hygrophila eggs can be achieved under the condition that the subsequent development stage, population and even next generation of the agasicles hygrophila eggs are not influenced.

Description

technical field [0001] The invention relates to a method for low-temperature preservation of insect eggs, in particular to a method for low-temperature preservation of eggs of A. chinensis. Background technique [0002] With the rapid development of the global integration process, invasive organisms have increased significantly, threatening global ecological security and biological security. China is one of the countries most severely affected by alien biological invasions. Among the more than 600 identified invasive alien species, invasive plants account for nearly half. This has become one of the important factors restricting the economic development of our country. Alternanthera philoxeroides is a global malignant weed. It was introduced into my country in the 1930s and has now spread to more than 20 provinces, municipalities, and autonomous regions across the country. Ecological and other serious threats, resulting in annual economic losses of up to 600 million yuan. A...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that after the existing A. chinensis eggs are cryopreserved, it is impossible to determine the impact on the subsequent development stage, population and even the next generation of A. basilicum, the present invention provides a Low-temperature preservation method to achieve the longest preservation time for the eggs of A.

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