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A test strip for early esophageal squamous cell carcinoma screening in high-risk groups
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A technology of esophageal squamous cell carcinoma and test strips, which is applied in the field of medicine and biology, can solve the problems of rare applications, achieve high sensitivity and specificity, reduce mortality, and improve the detection rate
Active Publication Date: 2020-12-01
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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Problems solved by technology
However, the application of multiple tumor marker combined detection methods and corresponding kits for the early diagnosis of esophageal squamous cell carcinoma is still relatively rare
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Embodiment 1
[0050] Embodiment 1: the preparation of test strip
[0051] 1. Experimental materials
[0052] The bioactive raw materials used in the present invention are all commercially available products. Among them, CFHR3 monoclonal antibody was purchased from Shanghai Biological Yubo Biotechnology Co., Ltd., the article number is MAL329Hu21; CFHR3 polyclonal antibody was purchased from Shanghai Kemin Biotechnology Co., Ltd., article number K005454P; C1QTNF6 monoclonal antibody was purchased from Shanghai Qunji Biotechnology Co., Ltd. company, Cat. No. MAB16078; C1QTNF6 polyclonal antibody was purchased from Amigi Technology Co., Ltd., Cat. Aimeijie Technology Co., Ltd., catalog number H00005982-B01P.
[0053] PVC base plate, nitrocellulose membrane, glass fiber membrane, absorbent paper etc. are all existing commercially available products.
[0054] 2. Preparation of binding pads
[0055] (1) Preparation of colloidal gold:
[0056] Colloidal gold solution was prepared by trisodium...
Embodiment 2
[0080] Embodiment 2: the diagnostic value analysis of test strip
[0081] Use the test strip prepared in Example 1 of the present invention to detect the serum samples of patients with pathologically confirmed early esophageal squamous cell carcinoma and normal people, and evaluate and analyze the value of the test strip of the present invention for screening and diagnosis of early esophageal squamous cell carcinoma.
[0082] 1. Sample source
[0083] Serum samples were collected from the Henan Key Open Laboratory of Esophageal Squamous Cell Carcinoma in the First Affiliated Hospital of Zhengzhou University, including 80 samples of normal human serum (control group) and 80 serum samples of patients with early-stage esophageal squamous cell carcinoma (esophageal squamous cell carcinoma group). The sera of 80 normal people came from the healthy check-up population of the physical examination center of the laboratory's cooperative hospital, without any evidence of tumor-related. ...
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Abstract
The invention belongs to the field of medical biotechnology, and discloses a test strip for screening high-risk groups of early esophageal squamous cell carcinoma. The test strip includes a binding pad and a chromatography pad. The binding pad is coated with a capture antibody, and the capture antibody is CFHR3 The mixture of Antibody A, C1QTNF6 Antibody A, RFC2 Antibody A, CFHR3 Antibody A, C1QTNF6 Antibody A, and RFC2 Antibody A all have detectable markers; there are three detection lines and one quality control line on the chromatography pad, and three detection lines The fixed detection antibodies were CFHR3 antibody B, C1QTNF6 antibody B, and RFC2 antibody B; rabbit anti-mouse IgG was immobilized on the quality control line. The test strip of the present invention can quickly detect CFHR3, C1QTNF6, and RFC2 in human serum, realize the combined detection of three tumor markers, and can effectively detect esophageal squamous cell carcinoma according to the detection results, greatly improving the detection rate of early esophageal squamous cell carcinoma .
Description
technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a test strip used for early screening of esophageal squamous cell carcinoma in high-risk groups. Background technique [0002] Esophageal squamous cell carcinoma is one of the six most common malignant tumors in the world, and its global morbidity and mortality rate ranks sixth. China is the country with the highest incidence and mortality rate of esophageal squamous cell carcinoma. The incidence rate occupies the fourth place, which is 100 times higher than that in western countries. Of the 500,000 new cases of esophageal squamous cell carcinoma in the world every year, more than half occur in China. The epidemiological characteristics of esophageal squamous cell carcinoma in China have significant regional distribution differences (the incidence rate difference between high and low incidence areas is 500 times, especially Linzhou, Henan Province and it...
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Application Information
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/558G01N33/543
CPCG01N33/57407G01N33/54313G01N33/558
Inventor 王立东陈瑶宋昕赵学科王盼盼程锟王苒李贝韩雪娜范宗民常志伟
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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