Correction method for hormones in panda urine

A correction method, the panda's technology, applied in the direction of material inspection products, measuring devices, instruments, etc., can solve problems such as the inability to use hormone correction formulas, and achieve the effects of eliminating underrepresentation, good accuracy, and fast correction

Inactive Publication Date: 2020-10-16
CHENGDU RES BASE OF GIANT PANDA BREEDING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, in the management of giant pandas, it is often necessary to temporarily detect and correct hormone levels for specific individuals. For giant p

Method used

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  • Correction method for hormones in panda urine
  • Correction method for hormones in panda urine
  • Correction method for hormones in panda urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The present embodiment is a kind of correction method of giant panda urine estrogen, and this correction method comprises the following steps:

[0036] (a) Clean and dry the ground of the animal house; place the giant panda in the animal house. Once the giant panda urinates, collect the urine with a syringe, put it into a freezing tube and put it in the refrigerator immediately;

[0037] (b) adopting the original estrogen concentration detection method in the giant panda urine disclosed in the specific embodiment of the patent application whose application number is 201310092471.5 detects the original estrogen concentration in the giant panda urine; and adopts a urine hydrometer (model is PAL -10S, brand ATAGO) to measure the urine specific gravity of giant panda urine;

[0038] (c) Correct the original estrogen concentration according to the correction result=original estrogen concentration / Lg urine specific gravity / 500000 to obtain the estrogen correction result.

Embodiment 2

[0040] The present embodiment is a kind of calibration method of giant panda urine progesterone, and this calibration method comprises the following steps:

[0041] (a) Clean and dry the ground of the animal house; place the giant panda in the animal house. Once the giant panda urinates, collect the urine with a syringe, put it into a freezing tube and put it in the refrigerator immediately;

[0042](b) adopting the detection method of the original progesterone concentration in the giant panda urine disclosed in the specific embodiment of the patent application whose application number is CN201310092282.8 to detect the original progesterone concentration in the giant panda urine; and using a urine hydrometer (model is PAL-10S, brand ATAGO) to measure the urine specific gravity of giant panda urine;

[0043] (c) Correct the original progesterone concentration according to the correction result=original progesterone concentration / Lg urine specific gravity / 500000 to obtain the pr...

experiment example

[0055] In the Chengdu Research Base of Giant Panda Breeding, the urine of giant pandas in different periods of estrus was collected, and the correction methods in Examples 1-2 and Control Examples 1-2 were adopted respectively (both creatinine correction method and urine specific gravity correction method were used). method) respectively corrected the estrogen and progesterone values ​​in giant panda urine, and the calibration results are shown in Table 1 below;

[0056] Table 1 Correction results of hormones by different correction methods

[0057]

[0058]

[0059] Correlation analysis was carried out on the results of creatinine correction method and urine specific gravity correction method of estrogen and progesterone; the analysis results were as follows: figure 1 , figure 2 As shown in the figure, Ec / USG refers to the correction result of the same giant panda Mengmeng urine estrogen urine specific gravity using the method of this application; Ec / Cr refers to the ...

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Abstract

The invention discloses a correction method for hormones in panda urine. The correction method comprises the following steps: collecting panda urine; measuring the original hormone concentration and the urine specific gravity in the panda urine; correcting the original hormone concentration according to a correction result = original hormone concentration/Lg urine specific gravity/500,000 to obtain a correction result. According to the method, the urine specific gravity value measured by panda urine is directly subjected to hormone urine specific gravity correction after being subjected to function conversion; the problem that the representativeness of the average urine specific gravity value is insufficient is solved, urine collection and average urine specific gravity value calculation do not need to be conducted on a specific panda in advance, the hormone level is directly corrected through a formula only after urine specific gravity measurement is conducted on the specific urine, and the correction method is easy to operate, rapid in correction and good in accuracy.

Description

technical field [0001] The invention relates to the technical field of giant panda urine hormone correction, in particular to a giant panda urine hormone correction method. Background technique [0002] The concentration of substances in urine is affected by drinking water, urine volume, food and other factors. In order to eliminate this effect, it can be corrected by collecting the subject's 24-hour urine method, flow rate correction method, creatinine correction method and specific gravity correction method, but the collection subject The 24-hour urine method and the flow rate correction method are inconvenient to operate, so they are rarely used. [0003] In the management of giant pandas in captivity, by detecting the levels of estrogen, progesterone and other reproductive hormones in urine samples of corresponding individual giant pandas in different time periods, and comparing and analyzing the changes of estrogen, progesterone and other related hormones in different t...

Claims

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Application Information

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IPC IPC(8): G01N9/36G01N21/31G01N33/74G01N33/70
CPCG01N9/36G01N21/31G01N33/74G01N33/70
Inventor 黄河侯蓉张志和胡贤彪张东升王成东兰景超黄祥明
Owner CHENGDU RES BASE OF GIANT PANDA BREEDING
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