Virus particles for preventing and controlling wheat stem rot and method for controlling wheat stem rot
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A technology of virus particles, stem rot, applied in the field of virology
Active Publication Date: 2022-05-20
HENAN AGRICULTURAL UNIVERSITY
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[0004] The invention proposes virus particles for preventing and treating wheat stalk rot and the method for preventing and treating wheat stalk rot, which solves the problem of preventing and treating wheat stalk rot in the prior art
Method used
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Embodiment 1
[0025] Study on biological control of crude extract of virus particle FpgMBV1
[0026] 1. Crude extraction of FpgMBV1 virus particles
[0027] 1. On the ultra-clean workbench, use a puncher to punch the bacteria cake, put the bacteria cake of Fusarium graminearum strain FC136-2A in the liquid medium PDB, and put it in a shaker at 25°C and 250rpm for 2 weeks.
[0028] 2. Take out the PDB medium after shaking culture, collect FC136-2A mycelium by filtering with gauze on the ultra-clean workbench, put the collected FC136-2A mycelium into a mortar, freeze and grind it into powder with liquid nitrogen.
[0029] 3. Transfer the FC136-2A mycelial powder into a sterilized Erlenmeyer flask, weigh the weight of the FC136-2A mycelial powder, add 31ml of PBS buffer solution to dissolve the FC136-2A mycelial powder per gram of mycelial powder, and then put the cone The flask was shaken in a shaker for 3 hours (10° C., 90 rpm) to release FpgMBV1 virions.
[0030] 4. Centrifuge the solutio...
Embodiment 2
[0052] 1. Extraction of FpgMBV1 Virus Particles
[0053] 1. Fusarium graminearum strain FC136-2A carrying FpgMBV1 virions was shaken in liquid medium PDB at 25°C and 250rpm for 2 weeks.
[0054] 2. Collect the mycelium by filtering with gauze on the ultra-clean workbench, freeze and grind it quickly with liquid nitrogen to powder (about 20g) and add it to a sterilized 500ml Erlenmeyer flask.
[0055] 3. Add 100ml of sodium phosphate solution (0.1mol / l, PH=7.0), put it in a 4°C refrigerator and stir for 2 hours with a magnetic suspension stirrer, and mix well.
[0056] 4. Dispense the mixed liquid in the Erlenmeyer flask into sterilized centrifuge tubes, centrifuge at 3500rpm at low speed for 20min at 4°C, and remove large impurities.
[0057] 5. Use a pipette gun to draw the supernatant into a new centrifuge tube, add an equal volume of chloroform, gently rotate the centrifuge tube to mix the mixture evenly, then centrifuge at 12000rpm, 4°C for 20min, and draw the supernatant...
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Abstract
The invention proposes a method for preventing and controlling the virus particles of Fusarium graminearum and the main pathogen of Fusarium graminearum of wheat stalk rot, and solves the problem of preventing and treating the stalk rot of wheat in the prior art. The present invention extracts FpgMBV1 virus particles, and carries out biological control treatment to wheat seedlings inoculated with Fusarium graminearum WZ2‑8A bacterial strain (a strong pathogenic strain of wheat stem base rot) indoors to determine the biological control potential of FpgMBV1 virus particles; The FpgMBV1 virions were co-cultured with the Fusarium pseudograminee WZ2-8A strain to verify the in vitro infection ability of FpgMBV1 virions. The results showed that FpgMBV1 virus particles could reduce the incidence of wheat stem rot disease infected by WZ2‑8A strain from 88.9% to 63.90%, the disease index from 36.73 to 7.10, and the control effect was 80.67%. It has biological control potential.
Description
technical field [0001] The invention relates to the field of virology, in particular to a virus particle for preventing and treating wheat stem rot and a method for preventing and treating wheat stem rot. Background technique [0002] In recent years, due to changes in farming systems such as farmland soil salinization and large-scale straw returning, the accumulation of Fusarium in farmland soil has continued, so the stem rot disease of wheat caused by various Fusarium species has increased year by year. The harm to wheat yield is also worthy of our attention (Tian Zhong et al., 2015). The symptoms of wheat stalk rot are mainly that after the wheat seeds in the soil are infected, they will rot before germination; when the wheat seedlings are infected, brown spots will appear on the base sheath and stalk of the wheat stem, which will then cause the wheat roots to rot. Browning and rot, when the disease is severe, the wheat leaves turn yellow and wither and die; when the dis...
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