Application of protease activated receptor 2 as cervical cancer metastasis detection marker

A protease activation, cervical cancer technology, applied in the field of cervical cancer detection, can solve the problems of lack of cervical cancer metastasis detection scheme, difficulty in accurately predicting cervical cancer metastasis, unfavorable cervical cancer treatment, control and prognosis assessment, etc.

Inactive Publication Date: 2021-01-15
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, there is currently a lack of effective cervical cancer metastasis detection schemes, and it is difficult to accurately

Method used

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  • Application of protease activated receptor 2 as cervical cancer metastasis detection marker
  • Application of protease activated receptor 2 as cervical cancer metastasis detection marker
  • Application of protease activated receptor 2 as cervical cancer metastasis detection marker

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Experimental program
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Effect test

Embodiment

[0045] 1. Materials and methods

[0046] 1. Main material

[0047] Tissue chip was purchased from Outdo Biotech Co., Ltd; rabbit anti-human polyclonal antibody PAR2 was purchased from Abcam Co., Cambridge, MA, USA; two-step Poly-HRP anti-mouse / rabbit IgG detection system was purchased from Wuxi OriGene; 119 samples of cervical cancer patients were provided by Shenzhen Second People's Hospital.

[0048] 2. PAR2 expression level and cervical cancer metastasis test

[0049] PAR2 immunohistochemical study on cervical cancer tissue microarray. All specimens were classified according to the tumor metastasis TNM classification system, and the steps of immunohistochemistry were as follows:

[0050] (1) Baked slices: 75°C, 30 minutes;

[0051] (2) Dewaxing: TO (I) soaking, 15 minutes; TO (II) soaking, 15 minutes;

[0052] (3) Rehydration: soak in 100% ethanol for 1 minute; soak in 90% ethanol for 1 minute; soak in 80% ethanol for 1 minute; soak in 70% ethanol for 1 minute; soak i...

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Abstract

The invention discloses an application of a protease activated receptor 2 as a cervical cancer metastasis detection marker. According to the application, the new cervical cancer metastasis detection marker, namely the protease activated receptor 2, is found through the research, and by detecting the expression condition of the protease activated receptor 2, the cervical cancer metastasis can be accurately and effectively detected, the cervical cancer metastasis part can be predicted, and the cervical cancer metastasis risk can be evaluated; and a new scheme is provided for cervical cancer detection, treatment and prognosis.

Description

technical field [0001] The present application relates to the technical field of cervical cancer detection, in particular to the application of protease-activated receptor 2 as a marker for cervical cancer metastasis detection. Background technique [0002] Metastasis is the main feature of malignant tumors and the main reason that affects the treatment effect and prognosis of patients. In addition to the genetic background of cancer cells, changes in the microenvironment have emerged as important factors regulating the progression of tumor metastasis. Over the past decade, increasing attention has been paid to the microenvironment surrounding cancer cells and its role in tumor metastasis. The microenvironment at the tumor invasive front may enable cancer cells to acquire abnormally high motility and penetrate the surrounding stroma. It is worth noting that the infiltration front of tumors is rich in various proteases, which promote tumor invasion and metastasis and cell m...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/68
CPCG01N33/57411G01N33/57484G01N33/74G01N2333/726
Inventor 何盛南杨澜许美权
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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