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Evaluation method for steady-state storage of milk sleep-promoting peptide

An evaluation method, a technology for promoting sleep, applied in the direction of testing dairy products, weighing by removing a certain component, testing food, etc., can solve the problem of no significant change, etc.

Pending Publication Date: 2022-02-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem of stable storage of milk sleep-promoting peptides, the present invention proposes an evaluation method, after measuring the ash content, pH value, and peptide content of different nodes of the stored milk sleep-promoting peptides, it is found that there is no significant change, It shows that milk sleep-promoting peptide has good stability, and the best storage condition is suitable temperature, sealed and dry

Method used

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  • Evaluation method for steady-state storage of milk sleep-promoting peptide
  • Evaluation method for steady-state storage of milk sleep-promoting peptide
  • Evaluation method for steady-state storage of milk sleep-promoting peptide

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0041] The steady-state storage method of bovine milk sleep-stimulating peptide was evaluated by the following steps:

[0042] (1) Preparation of milk sleep-promoting peptide samples

[0043] The cow's milk casein hydrolyzate was prepared into an aqueous solution with a content of 20wt%, and an appropriate volume of hydrochloric acid was added to adjust the pH value of the aqueous solution to 4. After standing for 10 minutes, it was centrifuged at a speed of 4000 rpm / min for 10 minutes to obtain supernatant and Precipitate, discard the precipitate and take the supernatant for later use. Add 80% ethanol aqueous solution to the supernatant, ethanol precipitation for more than 5 h, and centrifuge at 4000 rpm / min for 10 min to obtain the supernatant and precipitate, discard the precipitate and take the supernatant for later use. The supernatant is concentrated under reduced pressure at (60-65)°C until the solids are about 30wt%, and the solids are spray-dried using spray drying t...

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Abstract

The invention belongs to the technical field of milk-derived active ingredient extraction and particularly relates to an evaluation method for steady-state storage of the cow milk sleep-promoting peptide. The method comprises the following steps of (1) determining the ash content of the cow milk sleep-promoting peptide under different storage conditions; (2) measuring dry weight loss of the cow milk sleep promoting peptide under different storage conditions; (3) determining a pH value of the milk sleep promoting peptide under different storage conditions; and (4) determining the content of the milk sleep-promoting peptide under different storage conditions. The method is advantaged in that optimal storage condition of the cow milk sleep-promoting peptide is evaluated through a series of tests, and guidance is provided for a storage mode of the cow milk sleep-promoting peptide.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients from milk sources, and in particular relates to an evaluation method for stable storage of bovine milk sleep-promoting peptides. Background technique [0002] Sleep is an extremely important part of our life. Calculated according to the total length of life, we allocate more than one-third of our time to sleep. The health of the human body is directly related to the quality of sleep. Sleep is also a global hotspot in the field of health. A large number of studies have shown that the occurrence or development of many chronic diseases is closely related to sleep. Sleep disorders such as insufficient sleep time and insomnia may increase the risk of various chronic diseases such as diabetes, hypertension, obesity, heart and brain. The probability of occurrence of blood vessels, etc., and sleep time is related to the incidence of breast cancer, colorectal cancer, etc. According to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/04G01N5/04G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74
CPCG01N33/04G01N5/04G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N2030/324
Inventor 曹庸韦颖欣李俊刘星雨
Owner SOUTH CHINA AGRI UNIV
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