A sample extraction device for biological testing

Abstract

The invention relates to a sample extraction device for biological testing in the field of sample extraction devices. The sample extraction device comprises a test tube rack, a lifting rack is arranged on the outside, a control box is arranged on the fixed end, a horizontal guide rail is arranged on the top end, a longitudinal guide rail is arranged on the movable end, three kinds of mounting holes are arranged on the test tube rack, auxiliary extraction structures are arranged in the mounting holes, washing tubes and elution tubes are arranged in the other two mounting holes, the auxiliary extraction structure comprises a lifting platform, a supporting column and an annular magnetic column, the annular magnetic column has a plurality of pairs of arc-shaped electromagnets, the movable end of the longitudinal guide rail has an auxiliary extraction mechanism, comprising a shell, a motor and a stirring rod, the stirring rod has a main shaft, a segment block and an electromagnet ring, a control electric board is arranged in the shell, and image acquisition devices are arranged on the side end and the upper side of the test tube rack. By combining image recognition, automatic control and the design of the auxiliary extraction structure, the biomagnetic beads can efficiently adsorb biomass during the extraction process, and the biomagnetic beads are not easily missed during the transfer process, so that the biomagnetic beads can extract biomass efficiently, accurately and automatically.

Description

Technical Field

[0001] The present invention relates to a sample extraction device for biological testing, and in particular to a sample extraction device for biological testing applied in the field of sample extraction devices. Background Art

[0002] The sample extraction device for biological testing is a kind of experimental equipment specially used for sample extraction in biochemical testing. Biochemistry studies the chemical processes in organisms, which usually requires extracting samples from organisms or the environment for analysis.

[0003] In order to solve the problem of sample extraction stability, a certain sample extraction experimental equipment in the market adopts the design of mechanical arm assisted extraction operation, which has a certain market share.

[0004] In order to solve the problem of sample extraction, a certain sample extraction experimental equipment in the market adopts an efficient and automated design and has a certain market share.

[0005] The specification of Chinese invention patent CN115478072B discloses a rapid nucleic acid extraction method based on magnetic beads, which relates to the field of biotechnology. The rapid nucleic acid extraction method adopts a nucleic acid extraction kit, which includes a lysate, magnetic beads, a washing solution, an eluent, a nucleic acid protective agent, a deep-well plate, and a magnetic rod sleeve. The deep-well plate includes a box panel, a concave surface, a hole head, a threaded joint, a soft test tube, a torsion spring, a support plate, a bottom plate, a limit plate, a limit groove, an extension plate, and a magnetic strip. The surface of the box panel is provided with a concave surface, and the surface of the bottom plate is also provided with a limit groove. In the rapid nucleic acid extraction method based on magnetic beads, the soft test tube is flexible and can be folded, and the support plate can be rotated to the inside of the concave surface through the torsion spring, and the outer opening size of the box panel is matched with the inner opening size of the limit plate, so that the box panel and the folded soft test tube can be placed between the limit plates, thereby greatly reducing the volume of the deep-well plate for large-scale transportation.

[0006] In the existing biological detection process, after the biomass is extracted using biological magnetic beads, some biological magnetic beads are easily missed in the test tube during transfer. In addition, due to the small size of the biological magnetic beads, it is difficult to find them, which can easily lead to errors in the detection data and affect the detection results. Summary of the invention

[0007] In view of the above-mentioned prior art, the technical problem to be solved by the present invention is that in the existing biological detection process, after the biomass is extracted using biological magnetic beads, some biological magnetic beads are easily missed in the test tube during transfer, and since the biological magnetic beads are small in size and difficult to find, it is easy to cause errors in the detection data and affect the detection results.

[0008] In order to solve the above problems, the present invention provides a sample extraction device for biological testing, comprising a test tube rack, a lifting frame is installed outside the test tube rack, a control box is installed at the fixed end of the lifting frame, a horizontal guide rail is installed on the top of the lifting frame, and a longitudinal guide rail is installed on the movable end of the horizontal guide rail. Three types of mounting holes are opened on the test tube rack, auxiliary support structures are installed in the mounting holes, and washing tubes and elution tubes are placed in the other two mounting holes, and the washing tubes and elution tubes are used to hold detergent and eluent respectively;

[0009] The auxiliary support structure includes a lifting platform located at the bottom of the test tube rack, a support column is installed on the power output end of the lifting platform, and an annular magnetic column is installed on the fixed end of the lifting platform. The annular magnetic column includes multiple pairs of arc-shaped electromagnets matching the mounting holes;

[0010] An auxiliary extraction mechanism is installed on the movable end of the longitudinal guide rail, and the auxiliary extraction mechanism includes a shell, a motor is installed in the shell, and a stirring rod is connected to the power output end of the motor;

[0011] The stirring rod comprises a main shaft, on which are mounted a plurality of evenly distributed segment blocks with stirring blades, an electromagnet ring is connected between two adjacent segment blocks, and a waterproof layer is laid on the outer end of the electromagnet ring;

[0012] A control circuit board is installed in the housing, and a conductive slip ring electrically connected to a plurality of electromagnet rings is installed at the top of the main shaft, and the conductive slip ring is electrically connected to the control circuit board;

[0013] Image acquisition devices are installed on the side ends and upper side of the test tube rack.

[0014] In the above-mentioned biological test sample extraction device, by combining image recognition, automatic control and the design of auxiliary extraction structure, efficient and accurate extraction of biological samples is achieved, thereby improving the extraction efficiency.

[0015] As a further improvement of the present application, the image acquisition device acquires a top view image and a side view image of the liquid surface of the test tube placed in the test tube rack when working.

[0016] As a further improvement of the present application, the multiple pairs of arc-shaped electromagnets of the annular magnetic column have the same size, and the multiple pairs of arc-shaped electromagnets are stacked on each other to form a multi-layer structure. The annular magnetic column covers the upper part of the mounting hole, and the lower part of the mounting hole matches the movable range of the support column.

[0017] As a further improvement of the present application, the waterproof layer includes a flexible silicone waterproof layer, and the flexible silicone waterproof layer has a hollow structure, the outer wall of the flexible silicone waterproof layer matches the outer wall of the segment block, and the thickness of the flexible silicone waterproof layer is greater than the diameter of the biomagnetic beads.

[0018] As another improvement of the present application, an extraction control system is also included, which includes a processor arranged in a control box, and the processor is connected to a control module, an identification module and a verification module. The identification module is used to perform image recognition on images captured by the image acquisition device; the verification module is used to verify whether there are residual biological magnetic beads in the test tube.

[0019] As another improved supplement of the present application, the verification module is used to verify whether the biological magnetic beads are completely transferred from the test tube. When the verification module is working, the control module controls the auxiliary support structure to match the top of the annular magnetic column with the liquid surface of the test tube, and then controls the annular magnetic column to work so that the biological magnetic column is attracted to the liquid surface, and observes the liquid level and stratification status on the surface of the test tube through the image acquisition device.

[0020] A method for using a sample extraction device for biological testing, comprising the following steps:

[0021] A1, place the centrifuged test tube A into the test tube rack;

[0022] A2, using an image acquisition device to acquire an image of the test tube A, and identifying the volume of the supernatant and the volume of the turbid liquid in the test tube A;

[0023] A3, after extracting the supernatant through the liquid extraction device, add an equal volume of biomagnetic beads, put the biomagnetic beads into a mounting hole on the test tube rack, turn on the electromagnet in the test tube rack to assist the biomagnetic beads to quickly disperse and adsorb the biomass;

[0024] A4, after the adsorption setting time, the magnetic rod on the auxiliary extraction structure is used to adsorb the biological magnetic beads, and the electromagnet in the test tube rack is turned off; the magnetic rod adsorbs the biological magnetic beads and transfers them to the washing tube;

[0025] A5, the magnetic rod is powered off in the tube to release the biomagnetic beads, and then the auxiliary extraction structure is turned on to stir the biomagnetic beads to desorb the biomass from the biomagnetic beads;

[0026] A6, after stirring, the magnetic rod is turned on and absorbs the biological magnetic beads, and the magnetic rod is used to transfer the biological magnetic beads to the elution tube; after repeating step A5, the magnetic rod is used to transfer the biological magnetic beads to the recovery container.

[0027] As another improvement of the present application, after adding the biomagnetic beads to A3, a quantitative amount of deionized water is dripped onto the liquid surface.

[0028] In summary, this scheme combines image recognition, automated control and the design of auxiliary extraction structures to enable the biomagnetic beads to efficiently adsorb biomass during the extraction process and to prevent the biomagnetic beads from being missed during the transfer process, thereby achieving efficient, accurate and automated biomass extraction using biomagnetic beads. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a stereogram of the first and second embodiments of the present application;

[0030] Figure 2 It is a side cross-sectional view of the first and second embodiments of the present application;

[0031] Figure 3 This is a cross-sectional view of the stirring rod of the first and second embodiments of the present application when inserted into a test tube;

[0032] Figure 4 for Figure 3 The structural diagram at A in the middle;

[0033] Figure 5 This is a diagram showing the state changes of the biomagnetic beads in the test tube when the annular magnetic force is working in the first and second embodiments of the present application;

[0034] Figure 6 This is a flow chart of the method of use of the second implementation method of this application;

[0035] Figure 7 This is a diagram of the test tube state changes during the use of the second embodiment of the present application.

[0036] Description of the numbers in the figure:

[0037] 1 test tube rack, 2 lifting rack, 3 auxiliary extraction structure, 301 lifting platform, 302 supporting column, 303 annular magnetic column, 4 washing tube, 5 elution tube, 6 auxiliary extraction mechanism, 601 shell, 602 motor, 603 control board, 7 stirring rod, 701 main shaft, 702 segment block, 703 electromagnet ring, 704 conductive slip ring. DETAILED DESCRIPTION

[0038] The following describes two implementation modes of the present application in detail with reference to the accompanying drawings.

[0039] The first implementation method:

[0040] Figure 1-6 As shown, a sample extraction device for biological testing includes a test tube rack 1, a lifting frame 2 is installed outside the test tube rack 1, a control box is installed at the fixed end of the lifting frame 2, a horizontal guide rail is installed on the top of the lifting frame 2, and a longitudinal guide rail is installed on the movable end of the horizontal guide rail. Three types of mounting holes are opened on the test tube rack 1, and auxiliary support structures 3 are installed in the mounting holes. Washing tubes 4 and elution tubes 5 are placed in the other two mounting holes, and the washing tubes 4 and elution tubes 5 are used to hold detergents and eluents respectively;

[0041] The auxiliary support structure 3 includes a lifting platform 301 located at the bottom end of the test tube rack 1. The lifting platform 301 is used to hold up the test tube. A support column 302 is installed on the power output end of the lifting platform 301. An annular magnetic column 303 is installed on the fixed end of the lifting platform 301. The annular magnetic column 303 includes multiple pairs of arc-shaped electromagnets matching the mounting holes; the multiple pairs of arc-shaped electromagnets of the annular magnetic column 303 have the same size, and the multiple pairs of arc-shaped electromagnets are stacked on each other to form a multi-layer structure. The annular magnetic column 303 covers the upper part of the mounting hole, and the lower part of the mounting hole matches the range of motion of the support column 302.

[0042] By controlling the operation of each pair of arc-shaped electromagnets in each layer, the biomagnetic beads in the test tube in the auxiliary support structure 3 are attracted to different positions, so that the biomagnetic beads can be transferred and adsorbed in various regions in the test tube;

[0043] In this scheme, when the arc electromagnets of each layer are turned on and off in sequence from the lower layer (at this time, the upper arc electromagnet is turned on and then the adjacent lower arc electromagnet is closed), the biological magnetic beads in the test tube can be gradually led to the liquid surface of the test tube, so that the image acquisition device can perform image acquisition; and when the biological magnetic beads are on the liquid surface, the biological magnetic beads can be moved on the liquid surface by controlling the operation of multiple pairs of arc electromagnets, thereby causing the liquid surface fluid to fluctuate, making it easier to image the presence of biological magnetic beads on the liquid surface.

[0044] An auxiliary extraction mechanism 6 is installed on the movable end of the longitudinal guide rail, and the auxiliary extraction mechanism 6 includes a housing 601, a motor 602 is installed in the housing 601, and a stirring rod 7 is connected to the power output end of the motor 602;

[0045] The stirring rod 7 includes a main shaft 701 connected to the power output end of the motor 602, and a plurality of evenly distributed segment blocks 702 with stirring blades are installed on the main shaft 701. An electromagnet ring 703 is connected between two adjacent segment blocks 702, and a waterproof layer is laid on the outer end of the electromagnet ring 703; the waterproof layer includes a flexible silicone waterproof layer, and the flexible silicone waterproof layer has a hollow structure, the outer wall of the flexible silicone waterproof layer matches the outer wall of the segment block 702, and the thickness of the flexible silicone waterproof layer is greater than the diameter of the biological magnetic beads.

[0046] A control board 603 is installed in the housing 601, and a conductive slip ring 704 electrically connected to the plurality of electromagnet rings 703 is installed at the top of the main shaft 701, and the conductive slip ring 704 is electrically connected to the control board 603 through a wire;

[0047] Image acquisition devices are installed on the side ends and upper side of the test tube rack 1. When the image acquisition devices are working, they acquire top view images and side view images of the liquid surface of the test tubes placed in the test tube rack 1.

[0048] It also includes an extraction control system, which includes a processor arranged in a control box, and the processor is connected to a control module, an identification module and a verification module. The identification module is used to perform image recognition on images collected by the image acquisition device; the verification module is used to verify whether there are residual biological magnetic beads in the test tube.

[0049] The verification module is used to verify whether the biological magnetic beads are completely transferred from the test tube. When the verification module is working, the control module controls the auxiliary support structure 3 to work, so that the arc electromagnet at the top of the annular magnetic column 303 matches the liquid surface of the test tube, and then controls the annular magnetic column 303 to work so that the biological magnetic column is attracted to the liquid surface, and then controls multiple pairs of arc electromagnets at the top of the annular magnetic column 303 to work alternately, and observes the liquid surface condition and stratification condition of the test tube surface through an image acquisition device, and determines whether there are biological magnetic beads or obvious fluid fluctuations on the liquid surface of the test tube during the operation of the arc electromagnet by image recognition, so as to determine whether there are residual biological magnetic beads in the test tube.

[0050] The control module is also connected to a sample injector for adding test tube reagents and biological magnetic beads to the test tube rack 1. The sample injector can be set on the longitudinal guide rail or installed on other equipment that can achieve accurate movement and positioning, such as a robotic arm robot. The sample injector is provided with multiple output ports. The sample injector is connected to an external liquid storage device through a pipeline. The sample injector is used to automatically add biological magnetic beads, detergents or eluents to the test tubes at designated positions.

[0051] A metering unit is installed in the pipette, which measures the output of the pipette so as to accurately control the amount of liquid and biological magnetic beads added. The pipette pipeline is also equipped with a temperature control device so that the pipette can output liquid at a suitable temperature to improve the efficiency and quality of biological magnetic bead extraction.

[0052] The control module of this solution is set with an automatic working mode. In the automatic mode, the device will automatically complete the entire extraction process of adding samples (biomagnetic beads, detergents and eluents), annular magnetic column attraction, stirring, biomagnetic bead transfer and verification, which simplifies the operation steps, improves work efficiency, and the automated extraction process is easy to reduce errors caused by manual operation errors.

[0053] This embodiment introduces an extraction control system, which realizes the automated control of the sample extraction process through the coordinated work of the control module, the recognition module and the verification module. The recognition module can recognize the image captured by the image acquisition device and accurately determine the volume and stratification of the liquid in the test tube. The verification module can verify the extraction process to ensure that there are no residual biological magnetic beads in the test tube, thereby improving the accuracy and reliability of the extraction.

[0054] During the sample extraction process, the stirring rod is driven by a motor to rotate to achieve stirring and mixing of the biomagnetic beads. The setting of the electromagnet ring allows the stirring rod to absorb and accommodate the biomagnetic beads between two adjacent segment blocks 702, reducing the possibility of the biomagnetic beads falling off during the transfer process, and achieving safe transfer and recovery of the biomagnetic beads. This design not only simplifies the operation steps, but also improves the extraction efficiency.

[0055] Second implementation method:

[0056] Figure 6-7 A method for using a sample extraction device for biological testing is shown, which specifically includes the following steps:

[0057] A1, place the centrifuged test tube A into the test tube rack;

[0058] A2, using an image acquisition device to acquire an image of the test tube A, and identifying the volume of the supernatant and the volume of the turbid liquid in the test tube A;

[0059] A3, after extracting the supernatant through the liquid extraction device, add an equal volume of biological magnetic beads, and after the biological magnetic beads are put into a mounting hole on the test tube rack, turn on the electromagnet in the test tube rack to assist the biological magnetic beads to disperse quickly and adsorb biomass; after adding the biological magnetic beads, add a certain amount of deionized water on the liquid surface. The function of the deionized water is to make the upper area of ​​the test tube liquid surface clear, so that when the biological magnetic beads are attracted to the liquid surface, the image acquisition device can clearly capture the image of the biological magnetic beads;

[0060] A4, after the adsorption setting time, the magnetic suction rod on the auxiliary extraction structure is used to adsorb the biological magnetic beads, and the electromagnet in the test tube rack is turned off; the magnetic suction rod adsorbs the biological magnetic beads and transfers them to the washing tube 4;

[0061] A5, the magnetic rod is powered off in the tube to release the biomagnetic beads, and then the auxiliary extraction structure is turned on to stir the biomagnetic beads to desorb the biomass from the biomagnetic beads;

[0062] A6, after stirring, the magnetic rod is turned on and absorbs the biological magnetic beads, and the magnetic rod is used to transfer the biological magnetic beads to the elution tube 5; after repeating step A5, the magnetic rod is used to transfer the biological magnetic beads to the recovery container.

[0063] It should be noted that each time the biomagnetic beads are transferred out of the test tube, the verification module is started to verify the test tube to verify whether the biomagnetic beads in it are completely transferred. If not, after the auxiliary support structure 3 releases the transferred biomagnetic beads (at this time, the electromagnet ring at the corresponding position of the test tube works to attract the biomagnetic beads to the wall of the test tube), it is reset to the test tube to attract the biomagnetic beads again with maximum power and transfer them;

[0064] This solution can achieve efficient extraction of samples for biological testing and ensure the full extraction and purification of biomass. During the sample extraction process, the design of the auxiliary extraction structure enables the biomagnetic beads to disperse and adsorb biomass more quickly, thereby improving the extraction efficiency. At the same time, the image acquisition device can monitor the liquid in the test tube in real time to ensure the accurate addition of the supernatant and biomagnetic beads, as well as the effective operation of the biomagnetic beads during washing and elution.

[0065] The sample extraction device and method for biological testing of this scheme, by combining image recognition, automated control and the design of auxiliary extraction structure, enables the biomagnetic beads to efficiently adsorb biomass during the extraction process and is not easily missed during the transfer process, thereby achieving efficient, accurate and automated biomass extraction process using biomagnetic beads.

[0066] In view of current practical needs, the above-mentioned implementation mode adopted in this application is not limited to the scope of protection. Various changes made within the knowledge scope of technical personnel in this field without departing from the concept of this application still fall within the scope of protection of the present invention.

Claims

1. A sample extraction device for biological testing, comprising a test tube rack (1), a lifting frame (2) being installed outside the test tube rack (1), a control box being installed at a fixed end of the lifting frame (2), a horizontal guide rail being installed at the top end of the lifting frame (2), and a longitudinal guide rail being installed at a movable end of the horizontal guide rail, Features: The test tube rack (1) is provided with three types of mounting holes, wherein an auxiliary support structure (3) is installed in one of the mounting holes, and a washing tube (4) and an elution tube (5) are placed in the other two types of mounting holes, wherein the washing tube (4) and the elution tube (5) are used to hold detergent and eluent, respectively; The auxiliary support structure (3) comprises a lifting platform (301) located at the bottom end of the test tube rack (1), a support column (302) is installed on the power output end of the lifting platform (301), and an annular magnetic column (303) is installed on the fixed end of the lifting platform (301), and the annular magnetic column (303) comprises a plurality of pairs of arc-shaped electromagnets matching the mounting holes; An auxiliary extraction mechanism (6) is installed on the movable end of the longitudinal guide rail, and the auxiliary extraction mechanism (6) comprises a housing (601). An electric motor (602) is installed in the housing (601), and a stirring rod (7) is connected to the power output end of the electric motor (602); The stirring rod (7) comprises a main shaft (701), on which a plurality of evenly distributed segment blocks (702) with stirring blades are mounted, an electromagnet ring (703) is connected between two adjacent segment blocks (702), and the outer end of the electromagnet ring (703) is provided with a waterproof layer; A control electric board (603) is installed in the housing (601), a conductive slip ring (704) electrically connected to the plurality of electromagnet rings (703) is installed at the top end of the main shaft (701), and the conductive slip ring (704) is electrically connected to the control electric board (603); Image acquisition devices are installed on the side ends and upper side of the test tube rack (1).

2. A biological test sample extraction device according to claim 1, Features: When the image acquisition device is in operation, it acquires a top view image and a side view image of the liquid surface of the test tube placed in the test tube rack (1).

3. A biological test sample extraction device according to claim 1, Features: The multiple pairs of arc-shaped electromagnets of the annular magnetic column (303) have the same size, and the multiple pairs of arc-shaped electromagnets are stacked on each other to form a multi-layer structure; the annular magnetic column (303) covers the upper part of the mounting hole, and the lower part of the mounting hole matches the movable range of the support column (302).

4. A biological test sample extraction device according to claim 1, Features: The waterproof layer comprises a flexible silicone waterproof layer, and the flexible silicone waterproof layer is a hollow structure, the outer wall of the flexible silicone waterproof layer matches the outer wall of the segment block (702), and the thickness of the flexible silicone waterproof layer is greater than the diameter of the biomagnetic bead.

5. A biological test sample extraction device according to claim 1, Features: It also includes an extraction control system, which includes a processor arranged in a control box, and the processor is connected to a control module, an identification module and a verification module. The identification module is used to perform image recognition on images collected by the image acquisition device; the verification module is used to verify whether there are residual biological magnetic beads in the test tube.

6. A biological test sample extraction device according to claim 5, Features: The verification module is used to verify whether the biological magnetic beads are completely transferred from the test tube. When the verification module is in operation, the control module controls the auxiliary support structure (3) to work so that the top of the annular magnetic column (303) matches the liquid surface of the test tube, and then controls the annular magnetic column (303) to work so that the biological magnetic column is attracted to the liquid surface, and observes the liquid surface condition and stratification condition of the test tube surface through the image acquisition device.

7. A method for using the biological test sample extraction device according to claim 6, Features: The specific steps include: A1, place the centrifuged test tube A into the test tube rack; A2, using an image acquisition device to acquire an image of the test tube A, and identifying the volume of the supernatant and the volume of the turbid liquid in the test tube A; A3, after extracting the supernatant through the liquid extraction device, add an equal volume of biomagnetic beads, put the biomagnetic beads into a mounting hole on the test tube rack, turn on the electromagnet in the test tube rack to assist the biomagnetic beads to quickly disperse and adsorb the biomass; A4, after the set adsorption time, the magnetic rod on the auxiliary extraction structure is used to adsorb the biological magnetic beads. At this time, the electromagnet in the test tube rack is turned off; the magnetic rod adsorbs the biological magnetic beads and transfers them to the washing tube (4); A5, the magnetic rod is powered off in the tube to release the biomagnetic beads, and then the auxiliary extraction structure is turned on to stir the biomagnetic beads to desorb the biomass from the biomagnetic beads; A6, after stirring, the magnetic rod is turned on and absorbs the bio-magnetic beads, and the bio-magnetic beads are transferred to the elution tube (5) using the magnetic rod; after repeating step A5, the bio-magnetic beads are transferred to the recovery container using the magnetic rod.

8. A method for using a biological test sample extraction device according to claim 7, Features: After adding the biomagnetic beads to A3, a quantitative amount of deionized water is added dropwise onto the liquid surface.

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