Tumor tissue inoculation device

Abstract

The tumor tissue inoculation device comprises a supporting mechanism, a material conveying mechanism and a control assembly, and the supporting mechanism comprises a shell, telescopic assemblies on the two sides, a cover door movably connected with the front end of the shell, an internal connecting base, a connecting shaft and a handle; the material conveying mechanism comprises a bearing rotating sleeve, a rotating sleeve, a pressing sleeve, a connecting frame, a plurality of connecting sleeves, a tumor storage cylinder, a supporting sleeve, a pushing rod piece, a pressing assembly, a third electric pushing rod, a needle tube assembly and the like. The control assembly comprises a control panel, a battery module and a key module. Through the design of the multiple tumor storage barrels and the cooperation of the rotating sleeve and the pressing sleeve, automatic replacement of the tumor storage barrels is achieved. By means of the structure of the pushing rod piece, the ball and the clamping groove and automatic control of the electric pushing rod and the control assembly, tumor tissue blocks are accurately and quantitatively conveyed into the trocar, the experiment operation efficiency is improved, the labor intensity of experimenters is reduced, and important practical value is achieved for biomedical experiments.

Description

Technical Field

[0001] The invention belongs to the technical field of injections, and in particular relates to a tumor tissue inoculation device. Background Art

[0002] Tumor research plays an important role in the medical field, especially the exploration of tumor biology and treatment methods. Tumor inoculation technology, that is, implanting tumor cells or tissues into model organisms, is a key experimental technique widely used to study tumor growth, metastasis, drug response and treatment strategies.

[0003] The existing tumor tissue block inoculation technology is to load the tumor tissue into the cannula of an ordinary tissue block inoculation needle, and then inoculate it into the armpit of the experimental animal to form a tumor in the animal's armpit. Ordinary tissue inoculation needles usually use a cannula needle for tumor tissue inoculation, and can only load one or two pieces of tissue at a time. The loading speed is slow, time-consuming and laborious, and it is impossible to achieve quantitative tissue block volume and continuous inoculation of animals, resulting in low experimental efficiency.

[0004] Therefore, this paper proposes a tumor tissue inoculation device. Summary of the invention

[0005] The present invention designs a tumor tissue inoculation device to solve the problems existing in the above-mentioned background technology.

[0006] In order to achieve the above technical effects, the present invention is implemented through the following technical solutions: a tumor tissue inoculation device, including a supporting mechanism, a feeding mechanism and a control component.

[0007] Furthermore, the supporting mechanism includes an outer shell, a telescopic assembly arranged on both sides above the outer shell, a cover door rotatably connected to the telescopic assembly and movably connected to the front end of the outer shell, a connecting seat arranged inside the outer shell, a connecting shaft arranged on the connecting seat, and a handle arranged at the lower end of the outer shell; the feeding mechanism includes a bearing sleeve fixedly connected to the side end of the connecting seat, a rotating sleeve detachably connected to the bearing sleeve, a pressing sleeve movably connected to the rotating sleeve, a connecting frame fixedly sleeved on the bearing sleeve, a plurality of connecting sleeves arranged on the connecting frame, a storage tube movably connected to the connecting sleeve, a supporting sleeve arranged at the inner side end of the outer shell, a pushing rod slidably connected to the inside of the supporting sleeve, a pressing assembly arranged at the rear end of the outer shell and connected to the pushing rod, a third electric push rod arranged at the side end of the cover door, and a needle tube assembly arranged at the side end of the cover door; the control assembly includes a control panel arranged at the upper end of the outer shell, a battery module arranged inside the handle, and a button module arranged on the handle.

[0008] Furthermore, an insert block is symmetrically arranged at the lower end of the shell port, and the lower end of the shell port is detachably connected to the bottom of the cover door. A first electric push rod is provided on one side of the lower interior of the shell, and the upper end of the cover door is rotatably connected to the telescopic end of the telescopic assembly; a connecting groove is provided on the inner side of the cover door, and the outer end of the connecting shaft is detachably connected to a right-angle bracket, and the side end of the right-angle bracket is detachably connected to the connecting groove.

[0009] Furthermore, the pressing sleeve is disassembled and sleeved on the connecting shaft; a sliding groove ring is provided inside the rotating sleeve, a plurality of third grooves are provided at one end of the sliding groove ring, and a plurality of top teeth are provided at the other end, and the tooth tips of the top teeth are offset to one side of the groove opening of the third groove; a plurality of round blocks are evenly provided at the lower end of the pressing sleeve, and the round blocks are movably connected to the sliding groove ring, the number of the third grooves and the round blocks is the same, and the outer end of the pressing sleeve is fixedly connected to the pressing piece.

[0010] Furthermore, rolling grooves are symmetrically arranged on the upper and lower sides of the middle of the connecting sleeve, limiting sliding grooves are symmetrically arranged on the upper and lower sides of the front end of the connecting sleeve, first clamping grooves are symmetrically arranged on the upper and lower sides of the lower end of the connecting sleeve, and multiple second clamping grooves are symmetrically arranged on the left and right sides of the connecting sleeve.

[0011] Furthermore, limiting sliding blocks cooperating with limiting sliding grooves are provided on both sides of the middle of the tumor storage tube, first clamping blocks are provided on both sides of the bottom end of the tumor storage tube, a first ball is movably connected in the first annular groove of the first clamping block, a tube hole is provided at the bottom end of the tumor storage tube, a push plug is movably connected inside the tumor storage tube, and the front end of the tumor storage tube is disassembled and connected to the injection port.

[0012] Furthermore, the pushing rod includes a telescopic sleeve rod slidably connected to the inside of the supporting sleeve, a slot hole arranged on the front end surface of the telescopic sleeve rod, a through slot arranged on the upper and lower sides of the front end of the telescopic sleeve rod, a plurality of second annular grooves arranged on the left and right sides of the front end of the telescopic sleeve rod, a second ball movably connected in the second annular groove, a pushing rod movably connected to the inside of the telescopic sleeve rod, a pushing block arranged at the front end of the pushing rod and movably connected to the slot hole, and a retaining ring arranged at the front end of the telescopic sleeve rod and behind the pushing block.

[0013] Furthermore, the pressing assembly includes a shield arranged on the side of the rear end of the shell, and a second electric push rod arranged in the shield; the telescopic end of the second electric push rod penetrates into the interior of the shell and is fixedly connected to one end of the push rod.

[0014] Furthermore, the needle tube assembly includes a three-section electric push rod fixedly connected to one side of the cover door, a connecting sleeve fixedly connected to the second push rod of the three-section electric push rod, a trocar needle detachably connected to the other side of the cover door, a pressing sleeve movably installed in the trocar needle, a push needle movably installed in the pressing sleeve and the trocar needle, and a feed port arranged at the side end of the trocar needle, the feed port and the injection port of the tumor storage cylinder cooperate with each other, the rear end of the pressing sleeve is detachably connected to the connecting sleeve, and the rear end of the push needle is detachably connected to the front end of the first push rod of the three-section electric push rod.

[0015] The beneficial effects of the present invention are:

[0016] 1. The present invention adopts a multi-tumor storage tube design, which allows multiple tumor tissue blocks to be pre-filled. The automatic replacement of the tumor storage tube is achieved through the clever cooperation of rotating the sleeve and pressing the sleeve. This design reduces the steps of frequent manual replacement of the tumor storage tube and improves the efficiency of experimental operation. The structural design of the push rod, ball bearing and slot ensures that the tumor tissue block can be accurately delivered to the trocar. In addition, the combination of the electric push rod and the control component realizes the automatic control of the device and improves the accuracy and consistency of the inoculation process.

[0017] 2. The design of the telescopic component and the cover door in the present invention facilitates the placement and maintenance of the internal components of the device. The intelligent control of the control component enables the operator to monitor the working status of the device in real time and make timely operational adjustments. The device realizes the quantification of the volume of tumor tissue blocks and the continuous inoculation of mice through the organic combination of mechanical structure and intelligent control, thereby improving the efficiency and accuracy of tumor tissue inoculation, reducing the labor intensity of experimenters, and improving the reliability and repeatability of experimental results. It has important practical value for biomedical experiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2 It is a schematic diagram of the internal structure of the upper part of the present invention;

[0021] Figure 3 It is a right side view of the present invention;

[0022] Figure 4 The present invention Figure 3 Partial schematic diagram of the section at AA in the middle;

[0023] Figure 5 is a top view of the present invention;

[0024] Figure 6 The present invention Figure 5 Partial schematic diagram of the section at BB in the middle;

[0025] Figure 7 It is a schematic diagram of the internal structure of the present invention;

[0026] Figure 8 It is a schematic diagram of the disassembly of the internal structure of the present invention;

[0027] Fig. 9 This is a schematic diagram of the appearance structure of the tumor storage tube of the present invention;

[0028] Fig.10 The present invention Figure 8 A magnified schematic diagram of the structure at F in the middle;

[0029] Fig.11 It is a schematic diagram of the internal structure of the rotating sleeve of the present invention;

[0030] Fig.12 It is a schematic diagram of the internal structure of the rotating sleeve of the present invention;

[0031] Fig.13 It is a schematic diagram of the internal structure of the connecting sleeve of the present invention;

[0032] Fig.14 This is a schematic diagram of the initial state structure of the tumor storage tube of the present invention;

[0033] Fig.15 The present invention Fig.14 A magnified schematic diagram of the structure at B in the middle;

[0034] Fig.16 This is a schematic diagram of the structure of the tumor storage tube after it is pushed out;

[0035] Fig.17 The present invention Fig.16 A magnified schematic diagram of the structure at C in the middle;

[0036] Fig.18 The present invention Fig.17 Schematic diagram of the section at DD in the middle;

[0037] Fig.19 It is a schematic diagram of the structure of the present invention after the push rod pushes the tumor storage cylinder to transport materials;

[0038] Fig. 20 The present invention Fig.19 The enlarged schematic diagram of the structure at E in the middle;

[0039] Fig.21 It is a schematic diagram of the structure of the ball in the annular groove of the clamping block of the present invention;

[0040] Fig. 22 It is a schematic diagram of the movement process of the internal structure of the needle tube assembly of the present invention;

[0041] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0042] 1. Support mechanism; 11. Shell; 111. First electric push rod; 12. Telescopic assembly; 13. Cover door; 131. Connecting groove; 14. Connecting seat; 15. Connecting shaft; 1511. Right-angle bracket; 16. Handle; 2. Feeding mechanism; 21. Rotating sleeve; 211. Sliding groove ring; 2111. Third clamping groove; 2112. Top tooth; 22. Pressing sleeve; 221. Round block; 222. Pressing piece; 23. Connecting frame; 24. Connecting sleeve; 241. Rolling groove; 242. Limiting slide groove; 243. First clamping groove; 244. Second clamping groove; 25. Storage tube; 251. Limiting slide block; 252. First clamping block; 2 53. First ball bearing; 254. Push plug; 255. Cylinder hole; 26. Support sleeve; 27. Propelling rod; 271. Telescopic sleeve; 272. Second ball bearing; 273. Push rod; 274. Slot hole; 275. Through slot; 276. Push block; 277. Retaining ring; 28. Pressing assembly; 281. Protective cover; 282. Second electric push rod; 29. ​​Third electric push rod; 30. Bearing swivel sleeve; 3. Control assembly; 31. Control panel; 32. Battery module; 33. Button module; 4. Needle tube assembly; 41. Three-section electric push rod; 42. Connecting sleeve; 43. Sleeve needle; 44. Pressing sleeve; 45. Push needle; 46. Feed port. DETAILED DESCRIPTION

[0043] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] Example 1

[0045] See also Figures 1 to 22As shown, a tumor tissue inoculation device comprises a support mechanism 1, a feeding mechanism 2 and a control component 3, characterized in that: the support mechanism 1 comprises a shell 11, telescopic components 12 arranged on both sides above the shell 11, a cover door 13 rotatably connected to the telescopic components 12 and movably connected to the front end of the shell 11, a connecting seat 14 arranged inside the shell, a connecting shaft 15 arranged on the connecting seat 14, and a handle 16 arranged at the lower end of the shell 11; the feeding mechanism 2 comprises a bearing rotating sleeve 30 detachably connected to the front end of the connecting seat 14, a rotating sleeve 21 detachably connected to the bearing rotating sleeve 30 through a slot structure, a pressing sleeve 22 movably connected to the rotating sleeve 21, and a fixed sleeve 15 connected to the rotating sleeve 16. A connecting frame 23 on the cylinder 21, a plurality of connecting sleeves 24 arranged on the connecting frame 23, a tumor storage cylinder 25 movably connected in the connecting sleeve 24, a supporting sleeve 26 arranged at the inner side end of the shell 12, a pushing rod 27 slidably connected inside the supporting sleeve 26, a pressing assembly 28 arranged at the rear end of the shell 11 and connected to the pushing rod 27, a third electric push rod 29 arranged on the inner side of the cover door 13, a second pressing assembly 29 arranged at the side end of the cover door 13, and a needle tube assembly 4 arranged at the side end of the cover door 13; the control assembly 3 includes a control panel 31 arranged at the upper end of the shell 11, a battery module 32 arranged inside the handle 16, and a button module 33 arranged on the handle 16.

[0046] The side end of the connecting seat 14 is provided with a slot, and one side of the bearing rotating sleeve 30 is provided with an insert block that matches the slot, so as to realize the detachable connection between the connecting seat 14 and the bearing rotating sleeve 30. Linear slide rails can be arranged on both sides of the interior of the supporting sleeve 26, and linear sliders that match the linear slide rails are arranged at both ends of the pushing rod 274, so that the sliding connection between the supporting sleeve 26 and the pushing rod 274 can be realized.

[0047] The side end of the bearing rotating sleeve 30 is provided with an annular slide rail, and the side end of the rotating sleeve 21 is provided with a sliding block matched with the annular slide rail.

[0048] The lower end of the port of the housing 11 is detachably connected to the lower part of the cover door 13 through a snap assembly, a first electric push rod 111 is provided at one side of the lower part of the housing 11, and the upper end of the cover door 13 is rotatably connected to the telescopic end of the telescopic assembly 12 through a rotating shaft; a connecting groove 131 is provided on the inner side of the cover door 13, and the outer end of the connecting shaft 15 is detachably connected to a right-angle bracket 151 through a slot or bolt, and the right-angle bracket 151 supports the connecting shaft 15. The side end of the right-angle bracket 151 is detachably connected to the connecting groove 131.

[0049] The pressing sleeve 22 is disassembled and sleeved on the connecting shaft 15 through a linear slide rail; a sliding groove ring 211 is provided inside the rotating sleeve 21, and a plurality of third grooves 2111 are provided at one end of the sliding groove ring 211, and a plurality of top teeth 2112 are provided at the other end, and the tooth tips of the top teeth 2112 are offset to one side of the notch of the third groove 2111; a plurality of round blocks 221 are evenly provided at the lower end of the pressing sleeve 22, and the round blocks 221 are movably connected to the sliding groove ring 211, and the number of the third grooves 2111 and the round blocks 221 are the same; the outer end of the pressing sleeve 22 is fixedly connected to a pressing piece 222, and the telescopic end of the third electric push rod 29 will press the pressing piece 222 after being started.

[0050] Rolling grooves 241 are symmetrically arranged on the upper and lower sides of the middle of the connecting sleeve 24, limiting sliding grooves 242 are symmetrically arranged on the upper and lower sides of the front end of the connecting sleeve 24, first clamping grooves 243 are symmetrically arranged on the upper and lower sides of the lower end of the connecting sleeve 24, and multiple second clamping grooves 244 are symmetrically arranged on the left and right sides of the connecting sleeve 24.

[0051] The two sides of the middle of the tumor storage tube 25 are provided with limiting sliders 251 that match the limiting slide groove 242. The two sides of the bottom of the tumor storage tube 25 are provided with first clamping blocks 252. The first ring groove of the first clamping block 252 is movably connected to the first ball 253. The bottom of the tumor storage tube 25 is provided with a barrel hole 255. The structural principle of the tumor storage tube 25 is similar to that of the injection. The difference is that the inside of the tumor storage tube 25 is movably connected to the conical push plug 254. The front end of the tumor storage tube 25 is spirally connected to the conical injection port. When it is necessary to put the processed tumor tissue block into the tumor storage tube 25, the operator removes the injection port from the tumor storage tube 25 and ensures that the push plug 254 is under the tumor storage tube 25. Then put the tumor tissue block into the tumor storage tube 25. After the tumor tissue block is placed, the injection port is installed back on the tumor storage tube 25. According to the above operation, all tumor storage tubes 25 are filled with tumor tissue blocks.

[0052] The pushing rod 27 includes a telescopic sleeve 271 slidably connected to the inside of the supporting sleeve 26, a slot 274 arranged on the front end surface of the telescopic sleeve 271, a through slot 275 arranged on the upper and lower sides of the front end of the telescopic sleeve 271, a plurality of second annular grooves arranged on the left and right sides of the front end of the telescopic sleeve 271, a second ball 272 movably connected in the second annular groove, a pushing rod 273 movably connected to the inside of the telescopic sleeve 271, a pushing block 276 arranged at the front end of the pushing rod 273 and movably connected to the slot 274, and a retaining ring 277 arranged at the front end of the telescopic sleeve 271 and behind the pushing block 276.

[0053] The pressing assembly 28 includes a shield 281 disposed on the side of the housing 11 and a second electric push rod 282 disposed in the shield 281 ; the telescopic end of the second electric push rod 282 penetrates into the housing 11 and is fixedly connected to one end of the push rod 273 .

[0054] The needle tube assembly 4 includes a three-section electric push rod 41 fixedly connected to one side of the cover door 13, a sleeve 42 fixedly connected to the second push rod of the three-section electric push rod 41, a trocar 43 detachably connected to the other side of the cover door 13, a pressing sleeve 44 movably installed in the trocar 43, a push pin 45 movably installed in the pressing sleeve 44 and the trocar 43, and a feed port 46 arranged at the side end of the trocar 43, the feed port 46 matches the injection port of the tumor storage tube 25, the rear end of the pressing sleeve 44 is detachably connected to the sleeve 42, and the rear end of the push pin 45 is detachably connected to the front end of the first push rod of the three-section electric push rod 41 through a threaded structure. A pinhole structure matching the push pin 45 is arranged in the pressing sleeve 44.

[0055] The control panel 31 is provided with a display screen, and a central processing unit and an alarm are provided in the control panel 31. The central processing unit is electrically connected to the display screen, the alarm, the battery module 32, the key module 33, the three-section electric push rod 41, the first electric push rod 111, the second electric push rod 282, and the third electric push rod 291. The key module 33 includes a switch key, a start key, a stop key, a switch key, etc.

[0056] Example 2

[0057] The operating principle of the present invention:

[0058] When using the device to inoculate tumor tissue in the armpit of a mouse, first release the fixation between the outer shell 11 and the cover door 13, then stretch the telescopic component 12 of the device and rotate the cover door 13 to open it. Remove the right-angle bracket 151 on the connecting shaft 15, then take out the pressing sleeve 22 and the rotating sleeve 21, the connecting frame 23, the connecting sleeve 24, and the tumor storage tube 25 inside the outer shell 11, use relevant tools to push the tumor storage tube 25 out of 24, then open the injection port, fill the tumor tissue block in the tumor storage tube 25, and cover the injection port after filling. Repeat the above steps to fill each tumor storage tube 25 with tumor tissue blocks, then reinstall the removed components into the outer shell 11 and cover the cover door 13.

[0059] The operator starts the device by pressing the switch button and then presses the start button. After pressing the start button, the central processor receives the relevant signal and sends a start command to the second electric push rod 282. The telescopic rod of the second electric push rod 282 pushes the push rod 273 to move. Since the second ball 272 in the second ring groove is squeezed by the inner wall of the support sleeve 26 at this time, the second ball 272 in the second ring groove is offset inward. The structural principle is as follows Fig.21 As shown, the radius of the circular grooves on both sides of the annular groove is smaller than the diameter of the second ball 272, so when one side of the second ball 272 is squeezed, the second ball 272 will move to the circular groove on the other side, causing more ball parts to be exposed outside the squeezed side. Figure 7 As shown, these squeezed second balls 272 will block the push block 276 from passing through the slot 274. Therefore, under the push of the second electric push rod 282, the push block 276 will squeeze and push the second balls 272, so that the push rod 273 will push the telescopic sleeve rod 271 to move according to the telescopic length set by the second electric push rod 282.

[0060] like Figure 4 As shown, when the telescopic end of the first electric push rod 111 extends upward, the telescopic end of the first electric push rod 111 can prevent the tumor storage tube 25 from extending from the connecting sleeve 24. Fig.15 As shown, when the push rod 273 pushes the front end of the telescopic sleeve 271 into the support sleeve 26, due to the obstruction of the tumor storage tube 25 by the first electric push rod 111, the first ball 253 at the rear end of the tumor storage tube 25 is facing the first slot 243. When the front end through slot 275 of the telescopic sleeve 271 passes through the first ball 253, one side of the first ball 253 is squeezed, causing the first ball 253 to partially enter the first slot 243 on one side, so that the front end of the telescopic sleeve 271 smoothly passes through the first ball 253 and contacts the bottom surface of the tumor storage tube 25. Then, after the central processing unit sends a signal to the first electric push rod 111, the telescopic end of the first electric push rod 111 retracts and no longer blocks the tumor storage tube 25 from moving forward. Fig.17 As shown, the push rod 273 continues to move forward, and the telescopic sleeve 271 pushes the tumor storage tube 25 to move outward along the connecting sleeve 24. At this time, the first ball 253 is squeezed and disengages from the first clamping groove 243 and slides along the rolling groove 241. Limiting sliders 251 are provided on both sides of the tumor storage tube 25 to slide on the limiting sliding groove 242. When the front end of the tumor storage tube 25 extends out of the connecting sleeve 24, the central processor sends a stop command to the second electric push rod 282. At this time, the injection port of the tumor storage tube 25 is connected with the feed port 46 at the side end of the trocar 43, and the limiting slider 251 of the tumor storage tube 25 moves to the most side end of the limiting sliding groove 242 and is limited and no longer moves, and the multiple second balls 272 on the telescopic sleeve 271 are aligned with the second clamping groove 244 on the inner wall of the connecting sleeve 24, as shown in FIG. Fig.18 shown.

[0061] The telescopic end of the second electric push rod 282 continues to move the push rod 273, and the push block 276 presses the second ball 272, squeezing the other end of the second ball 272 into the second slot 244. Then the push block 276 smoothly passes through the slot 274, and then the push block 276 pushes the push plug 254 in the tumor storage tube 25 under the movement of the push rod 273, so that the tumor tissue block in the tumor storage tube 25 is injected into the trocar 43 from the injection port, until the push plug 254 is pushed to the injection port to inject all the tumor tissue blocks in the tumor storage tube 25 into the trocar 43, and the preparation stage of tumor inoculation is completed. After the preparation stage is completed, the central processor sends a signal to the alarm, and the alarm also prompts that the preparation stage is completed.

[0062] Then the operator passes the needle of the trocar 43 through the armpit of the mouse and presses the start button of the button module 33. The central processing unit sends a start signal to the three-section electric push rod 41. The second push rod of the three-section electric push rod 41 drives the first push rod to move telescopically together, but at this time the first push rod does not move inside the second push rod, and the front end of the push needle 45 cooperates with the front end of the pressure sleeve 44. The connecting sleeve 42 on the second push rod pushes the pressure sleeve 44 to move inside the trocar 43, pushing the tumor tissue block in the trocar 43 into the needle. Then the first push rod drives the push needle 45 to move, and the push needle 45 delivers the tumor tissue block in the needle into the mouse for inoculation.

[0063] When the second push rod and the third push rod of the three-section electric push rod 41 reach the maximum stroke, it indicates that the tumor tissue in the needle has been completely injected into the mouse. The central processor will send a signal to the alarm, and after the alarm sounds, the display screen will display that the tumor tissue block in the tumor storage tube 25 has been injected. The operator presses the switch key of the key module 33, and then the telescopic end of the second electric push rod 282 retracts, and the push rod 273 also moves back. When the pushing rod 273 moves into the telescopic sleeve 271, the pushing rod 273 presses the pushing retaining ring 277 to reset the entire pushing rod 27 to 26. During this process, the through groove 275 of the pushing rod 27 will drive the first ball 253 to move, thereby causing the tumor storage tube 25 to move back. When the first ball 253 reaches the position of the first slot 243, the limiting slider 251 also slides to the starting point of the limiting slot 242. The tumor storage tube 25 is limited and no longer moves. After the through groove 275 of the pushing rod 27 squeezes the other end of the first ball 253 into 243, the pushing rod 27 is separated from the tumor storage tube 25 and returns to the supporting sleeve 26.

[0064] The CPU sends a start command to the third electric push rod 29. The telescopic end of the third electric push rod 29 presses the pressing piece 222, so that the pressing sleeve 22 is pressed down into the rotating sleeve 21. The round block 221 on the pressing sleeve 22 is disengaged from the third slot 2111 after being pressed, and presses the side end of the top tooth 2112. Since the top tooth 2112 is offset from the center of the round block 221, the round block 221 moves along the track of the top tooth 2112 after being pressed down. Fig.12 As shown in the track, when the round block 221 is pressed down, the round block 221 moves along the direction from a to b. When the hand is released, 221 is no longer pressed downward, and 221 moves along the direction from b to c to enter the upper side of the next top tooth 2112. Since the pressing sleeve 22 slides linearly on the connecting shaft 15, and the rotating sleeve 21 and the bearing rotating sleeve 30 are in a rotational connection, each time the pressing sleeve 22 is pressed, the rotating sleeve 21 rotates once after being pressed, so that an exhausted tumor storage cylinder 25 rotates, and another tumor storage cylinder 25 filled with tumor tissue blocks rotates to the position of the supporting sleeve 26. After the pressing sleeve 22 is pressed to rotate the rotating sleeve 21 to complete the replacement of the tumor storage cylinder 25, the operator clicks the display screen to confirm. After receiving the relevant signal, the central processor sends a signal to the first electric push rod 111, and the telescopic end of the first electric push rod 111 moves downward to the front of the tumor storage cylinder 25, entering the preparation stage of tumor inoculation of the device. After the preparation stage is completed, the mouse is inoculated with tumors again. When the tumor storage tube 25 is used up again, the pressing sleeve 22 is continued to be pressed to rotate the rotating sleeve 21 to complete the replacement of the tumor storage tube 25, and the operation is repeated until the tumor tissue blocks in the multiple tumor storage tubes 25 in the device are used up, and then they can be taken out for filling. The push rod and ball design in the device allows automatic retraction and reset, so that the tumor storage tube can automatically return to the initial position after injection.

Claims

1. A tumor tissue inoculation device, comprising a support mechanism (1), a feed mechanism (2) and a control component (3), characterized in that: The support mechanism (1) comprises a housing (11), telescopic components (12) arranged on both sides above the housing (11), a cover door (13) rotatably connected to the telescopic components (12) and movably connected to the front end of the housing (11), a connecting seat (14) arranged inside the housing, a connecting shaft (15) arranged on the connecting seat (14), and a handle (16) arranged at the lower end of the housing (11); the feeding mechanism (2) comprises a bearing rotating sleeve (30) fixedly connected to the side end of the connecting seat (14), a rotating sleeve (21) detachably connected to the bearing rotating sleeve (30), a pressing sleeve (22) movably connected to the rotating sleeve (21), a connecting frame (23) fixedly sleeved on the rotating sleeve (21), and a connecting shaft (15) arranged on the connecting seat (14). A plurality of connecting sleeves (24) on a connecting frame (23), a tumor storage tube (25) movably connected in the connecting sleeve (24), a supporting sleeve (26) arranged at the inner side end of the outer shell (12), a pushing rod (27) slidably connected in the inner part of the supporting sleeve (26), a pressing assembly (28) arranged at the rear end of the outer shell (11) and connected to the pushing rod (27), a third electric push rod (29) arranged on the inner side of the cover door (13), and a needle tube assembly (4) arranged at the side end of the cover door (13); the control assembly (3) comprises a control panel (31) arranged at the upper end of the outer shell (11), a battery module (32) arranged in the inner part of the handle (16), and a key module (33) arranged on the handle (16).

2. A tumor tissue inoculation device according to claim 1, characterized in that: The lower end of the port of the shell (11) is detachably connected to the lower part of the cover door (13); a first electric push rod (111) is provided on one side of the lower part of the shell (11); the upper end of the cover door (13) is rotatably connected to the telescopic end of the telescopic assembly (12); a connecting groove (131) is provided on the inner side of the cover door (13); the outer end of the connecting shaft (15) is detachably connected to a right-angle bracket (151); and the side end of the right-angle bracket (151) is detachably connected to the connecting groove (131).

3. A tumor tissue inoculation device according to claim 1, characterized in that: The pressing sleeve (22) is detachably sleeved on the connecting shaft (15); a sliding groove ring (211) is provided inside the rotating sleeve (21); one end of the sliding groove ring (211) is provided with a plurality of third clamping grooves (2111), and the other end is provided with a plurality of top teeth (2112), and the tooth tips of the top teeth (2112) are offset to one side of the notch of the third clamping groove (2111); a plurality of round blocks (221) are evenly provided at the lower end of the pressing sleeve (22), and the round blocks (221) are movably connected to the sliding groove ring (211), the number of the third clamping grooves (2111) and the number of the round blocks (221) are the same, and the outer end of the pressing sleeve (22) is fixedly connected to the pressing piece (222).

4. A tumor tissue inoculation device according to claim 1, characterized in that: Rolling grooves (241) are symmetrically arranged on the upper and lower sides of the middle of the connecting sleeve (24), limiting sliding grooves (242) are symmetrically arranged on the upper and lower sides of the front end of the connecting sleeve (24), first clamping grooves (243) are symmetrically arranged on the upper and lower sides of the lower end of the connecting sleeve (24), and a plurality of second clamping grooves (244) are symmetrically arranged on the left and right sides of the connecting sleeve (24).

5. A tumor tissue inoculation device according to claim 1, characterized in that: The middle two sides of the tumor storage tube (25) are provided with limiting sliding blocks (251) matched with the limiting sliding groove (242); the bottom two sides of the tumor storage tube (25) are provided with first clamping blocks (252); the first ring groove of the first clamping block (252) is movably connected to the first ball (253); the bottom of the tumor storage tube (25) is provided with a tube hole (255); the inside of the tumor storage tube (25) is movably connected to a push plug (254); the front end of the tumor storage tube (25) is disassembled and connected to the injection port.

6. A tumor tissue inoculation device according to claim 1, characterized in that: The propulsion rod (27) comprises a telescopic sleeve (271) slidably connected inside the support sleeve (26), a slot (274) arranged on the front end surface of the telescopic sleeve (271), a through slot (275) arranged on the upper and lower sides of the front end of the telescopic sleeve (271), a plurality of second annular grooves arranged on the left and right sides of the front end of the telescopic sleeve (271), a second ball (272) movably connected in the second annular groove, a push rod (273) movably connected inside the telescopic sleeve (271), a push block (276) arranged at the front end of the push rod (273) and movably connected to the slot (274), and a retaining ring (277) arranged at the front end of the telescopic sleeve (271) and behind the push block (276).

7. A tumor tissue inoculation device according to claim 1, characterized in that: The pressing assembly (28) comprises a shield (281) arranged on the side of the housing (11) and a second electric push rod (282) arranged in the shield (281); the telescopic end of the second electric push rod (282) penetrates into the interior of the housing (11) and is fixedly connected to one end of the push rod (273).

8. The tumor tissue inoculation device according to claim 1, characterized in that: The needle tube assembly (4) comprises a three-section electric push rod (41) fixedly connected to one side of the cover door (13), a connecting sleeve (42) fixedly connected to the second push rod of the three-section electric push rod (41), a trocar (43) detachably connected to the other side of the cover door (13), a pressing sleeve (44) movably installed in the trocar (43), a push needle (45) movably installed in the pressing sleeve (44) and the trocar (43), and a feed port (46) arranged at the side end of the trocar (43), the feed port (46) and the injection port of the tumor storage tube (25) are matched, the rear end of the pressing sleeve (44) and the connecting sleeve (42) are detachably connected, and the rear end of the push needle (45) is detachably connected to the front end of the first push rod of the three-section electric push rod (41).

9. A tumor tissue inoculation device according to claim 1, characterized in that: The control panel (31) is electrically connected to the battery module (32), the key module (33), the three-section electric push rod (41), the first electric push rod (111), the second electric push rod (282), and the third electric push rod (29).

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