Whitening and brightening composition and preparation method thereof
Through the synergy between natural plant extracts such as Sophora, Polyacanthus, Pyracantha, Ningxia wolfberry and nicotinamide, a safe and effective whitening and brightening composition was prepared, which solved the problem of using irritating chemical raw materials in existing whitening products, and achieved significant whitening and antioxidant properties.
Patent Information
- Application Number
- CN202510100102.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-22
- Publication Date
- 2025-03-14
AI Technical Summary
Existing whitening skin care products mostly use harsh chemical raw materials, making it difficult to find natural compositions that do not irritate human skin and effectively whiten.
The whitening and brightening composition is prepared by using natural plant extracts such as Sophora cinnabaria, pyracantha, Ningxia wolfberry, etc., synergistically interact with nicotinamide.
This composition not only has a significant whitening effect, but also has excellent antioxidant properties and the advantages of improving product safety.
Smart Images
Figure BDA0005253955830000041
Abstract
Description
Technical Field
[0001] The invention relates to skin care products, in particular to a whitening and brightening composition and a preparation method thereof. Background Art
[0002] The well-known whitening and freckle-removing raw materials are as follows: 1. Inhibiting melanin gene expression, such as whitening peptides; 2. Complexing copper ions, such as phytic acid, kojic acid derivatives, etc.; 3. Inhibiting the transfer of melanosomes to the stratum corneum, such as niacinamide; 4. Inhibiting melanin production, such as lactic acid, sodium lactate, etc.; 5. Accelerating tyrosinase degradation, such as linoleic acid, etc.; 6. Reducing and competitive tyrosinase activity, such as phenethylresorcinol, glycyrrhizin, etc.; 7. Reducing melanin, such as vitamin C and its derivatives.
[0003] However, there are few whitening products that use natural ingredients and do not irritate human skin. Summary of the invention
[0004] In view of the deficiencies in the prior art, one of the objects of the present invention is to provide a whitening and brightening composition, and the second object of the present invention is to provide a method for preparing the whitening and brightening composition.
[0005] One of the purposes of the present invention is achieved through the following technical solutions:
[0006] A whitening and brightening composition, comprising the following raw materials in parts by weight:
[0007] 5-8 parts of Sophora flavescens root extract, 5-8 parts of Phyllostachys bambusoides extract, 3-5 parts of Pyracantha fortuneana fruit extract, 3-5 parts of Lycium barbarum fruit extract and 20-30 parts of niacinamide.
[0008] Preferably, the preparation method of the Osmanthus fragrans extract comprises the following steps:
[0009] Roasting, water extraction and drying.
[0010] Preferably, the baking comprises: heating the mature main stem of the bamboo to 205-215°C under anaerobic conditions for baking, raising the temperature to 220°C within 10 seconds after a popping sound is heard from the mature main stem of the bamboo, keeping the temperature for 5-9 minutes, then quickly taking it out and placing it in a room temperature environment until it cools to room temperature, thereby obtaining a baked bamboo.
[0011] Preferably, the water extraction comprises: adding water to the baked bamboo shoots at a weight ratio of 1:13 to break the wall and crush them, then adding cellulase (enzyme activity ≥ 10000U / g) at 5% of the weight of the baked bamboo shoots, performing enzymolysis for 30-45 minutes under stirring to obtain an enzymolysis solution, inactivating the enzyme in the enzymolysis solution, filtering and obtaining the supernatant to obtain bamboo shoot liquid.
[0012] Preferably, the enzyme inactivation method is: heating the enzymatic solution to 80° C. and maintaining it for 1 minute, thereby utilizing high temperature to inactivate the cellulase.
[0013] Preferably, the drying comprises: drying the bamboo shoots liquid into powder to obtain the bamboo shoots extract.
[0014] Preferably, the powder has a moisture content of 9%. A lower moisture content is convenient for long-term storage.
[0015] The second object of the present invention is achieved by the following technical solutions:
[0016] A method for preparing a whitening and brightening composition comprises the following steps:
[0017] Step 1, weighing the raw materials;
[0018] Step 2, adding n-hexane to pulp the Sophora flavescens root extract, Osmanthus fragrans extract, Pyracantha fortuneana fruit extract and Lycium barbarum fruit extract in the raw materials at a weight of 3 times the sum of their weights;
[0019] Step 3, degreasing the slurry;
[0020] Step 4: Add niacinamide at room temperature and stir well to obtain a whitening and brightening composition.
[0021] Preferably, the degreasing comprises: subjecting the slurry to ultrasonic treatment at a frequency of 40000 Hz and a power of 60 W for 4 hours, and drying at 40-50° C. after filtering.
[0022] Preferably, the whitening and brightening composition may further be added with 5.4-11.2 parts by weight of deionized water, that is, the composition is made into a paste, which is convenient for packaging, transportation and use.
[0023] The beneficial effects of the present invention are as follows: natural plants such as Sophora flavescens and Osmanthus fragrans are used in conjunction with nicotinamide to inhibit the expression of tyrosinase, and experiments have shown that the plants have excellent antioxidant properties; in addition, the plant raw materials of the present invention are defatted, so that matrine cannot undergo saponification reaction with lipids, thereby preventing the Sophora flavescens components from producing matrin saponins during the production, storage and use of the composition, thereby improving the safety of the product. DETAILED DESCRIPTION
[0024] The following is further described in conjunction with specific implementation methods:
[0025] Example 1
[0026] The preparation of the whitening and brightening composition comprises the following steps:
[0027] Weigh 5 parts of Sophora flavescens root extract, 5 parts of Phyllostachys bambusoides extract, 3 parts of Pyracantha fortuneana fruit extract, 3 parts of Lycium barbarum fruit extract and 20 parts of niacinamide according to weight.
[0028] The Sophora flavescens root extract, Osmanthus fragrans extract, Pyracantha fortuneana fruit extract and Lycium barbarum fruit extract in the raw materials were slurried with n-hexane at 3 times the sum of their weights (using a stirring kettle at 200 r / min for 20 min).
[0029] The slurry was subjected to ultrasonic treatment at a frequency of 40,000 Hz and a power of 60 W for 4 h, and then filtered and dried at 40°C.
[0030] Add niacinamide at room temperature and stir well to obtain a whitening and brightening composition.
[0031] All the raw materials in this example were purchased through conventional channels.
[0032] Example 2
[0033] The preparation method of the bamboo shoot extract comprises the following steps:
[0034] The mature main stem of the bamboo is heated to 210°C under anaerobic conditions for baking. During the baking process, after a popping sound is heard from the mature main stem of the bamboo, the temperature is raised to 220°C within 10 seconds, kept warm for 8 minutes, and then quickly taken out and placed in a room temperature environment until it is cooled to room temperature, thereby obtaining a baked bamboo.
[0035] The baked cinnamon bamboo is crushed by adding water at a weight ratio of 1:13, and then cellulose enzyme (enzyme activity ≥ 10000U / g) is added at 5% of the weight of the baked cinnamon bamboo, and enzymolysis is performed for 40 minutes under stirring to obtain an enzymolysis solution, and the enzymolysis solution is inactivated by filtering and taking the supernatant to obtain cinnamon bamboo liquid. The enzyme inactivation method is: heating the enzymolysis solution to 80°C and maintaining it for 1 minute.
[0036] The cinnamon bark extract is dried into powder to obtain cinnamon bark extract, wherein the powder has a water content of 9%.
[0037] The preparation of the whitening and brightening composition comprises the following steps:
[0038] Weigh 5 parts of Sophora flavescens root extract, 5 parts of Osmanthus fragrans extract, 3 parts of Pyracantha fortuneana fruit extract, 3 parts of Lycium barbarum fruit extract and 20 parts of niacinamide according to weight.
[0039] The Sophora flavescens root extract, Osmanthus fragrans extract, Pyracantha fortuneana fruit extract and Lycium barbarum fruit extract in the raw materials were slurried with n-hexane at 3 times the sum of their weights (using a stirring kettle at 200 r / min for 20 min).
[0040] The slurry was subjected to ultrasonic treatment at a frequency of 40,000 Hz and a power of 60 W for 4 h, and then filtered and dried at 40°C.
[0041] Add niacinamide at room temperature and stir well to obtain a whitening and brightening composition.
[0042] In this example, all raw materials except the Osmanthus fragrans extract were purchased through conventional channels.
[0043] Example 3
[0044] The difference from Example 2 is that 8 parts of Sophora flavescens root extract, 8 parts of Osmanthus fragrans extract, 5 parts of Pyracantha fortuneana fruit extract, 5 parts of Lycium barbarum fruit extract and 30 parts of niacinamide are weighed according to weight.
[0045] Example 4
[0046] The difference from Example 2 is that 7 parts of Sophora flavescens root extract, 7 parts of Osmanthus fragrans extract, 4 parts of Pyracantha fortuneana fruit extract, 4 parts of Lycium barbarum fruit extract and 25 parts of niacinamide are weighed according to weight.
[0047] Example 5
[0048] The difference from Example 2 is that 7 parts by weight of deionized water are added to the whitening and brightening composition and stirred evenly.
[0049] Comparative Example 1
[0050] The Sophora flavescens root extract was replaced with an equal weight of Phellodendron amurense extract.
[0051] Tyrosinase activity assay
[0052] The purpose of the experiment is to verify that the whitening and brightening composition obtained by the present invention can inhibit the expression of tyrosinase.
[0053] Experimental method: take B16F10 cells in good exponential growth phase, digest, count, inoculate the cells in a 10 cm cell culture dish, the inoculation concentration is about 1×105 cells / mL, the inoculation volume is 10 mL / dish, and culture in a 37°C, 5% CO2 incubator for 24 hours; add 100 μL of the whitening and brightening composition of Examples 1-5 and Comparative Example 1, respectively, and culture for 72 hours; collect the cells after the above-mentioned drug administration treatment, add 100 μL of non-denaturing lysis solution (containing 1 mM PMSF), and lyse at 4°C for 20 minutes; centrifuge at 4°C, 12000 r / min for 10 minutes, and the supernatant is the total cell protein extract. After extracting cell protein, add 10 μg of total protein in the cell lysis supernatant to a centrifuge tube, add PBS (pH 6.8) to 100 μL, mix thoroughly and add to a 96-well plate, then add 0.1% L-DOPA 100 μL, set 3 replicates for each group, incubate at 37°C for 1 hour in the dark. Measure the absorbance at 475 nm. The larger the absorbance value, the higher the tyrosinase activity.
[0054] The experimental results are shown in Table 1.
[0055] Table 1 Tyrosinase activity statistics
[0056] sample Example 1 Example 2 Example 3 Example 4 Example 5 Comparative Example 1 active(%) 41 47 45 42 50 74
[0057] As shown in Table 1, when the Sophora flavescens root extract was replaced by the Phellodendron amurense extract having similar antibacterial function, the inhibitory effect on tyrosinase expression was greatly reduced. Since the Sophora flavescens root extract itself does not have an inhibitory effect on tyrosinase expression, it can be inferred that the Sophora flavescens root extract has a synergistic effect in inhibiting tyrosinase expression.
[0058] Antioxidant test
[0059] The purpose of the experiment is to verify that the whitening and brightening composition obtained by the present invention has an antioxidant effect.
[0060] Experimental method: Take 0.1g, 0.2g and 0.3g of the samples of Examples 1-4 and Comparative Example 1, add 2.0mL 1.52×10-4mmol / L DPPH anhydrous ethanol solution and mix thoroughly. After standing at room temperature and away from light for 30min, take the solvent as blank control and measure the absorbance A1 at 517nm; use the same volume of solvent instead of the sample solution as a negative control to measure the absorbance A2; use the same volume of anhydrous ethanol instead of DPPH solution as a blank group to measure the absorbance A3. Take VC as the control, do 3 replicates for each group, and calculate the average value. Calculate the clearance rate according to the following formula: Sample clearance rate of DPPH (%) = [1-(A1-A3) / A2]×100%.
[0061] The experimental results are shown in Table 2.
[0062] Table 2 DPPH free radical scavenging rate statistics
[0063]
[0064] As shown in Table 2, when the Sophora flavescens root extract was replaced by the Phellodendron amurense extract having similar antibacterial function, the DPPH free radical scavenging rate decreased significantly, so it can be inferred that the Sophora flavescens root extract had a synergistic effect on antioxidant activity.
[0065] Antibacterial test
[0066] The purpose of the experiment is to verify that the whitening and brightening composition obtained by the present invention has an antibacterial effect.
[0067] Experimental method: inoculate the strain (Staphylococcus aureus) on an agar plate, place it in a constant temperature incubator at 37°C and culture it for 24h, select a single colony and inoculate it on an agar plate, place it in a constant temperature incubator at 37°C and culture it for 16-18h, the strain concentration is equivalent to 1×108CFU / mL, dilute it 1000 times with beef extract peptone liquid medium as a diluent, so that the concentration of the diluted bacterial solution is about 1×105CFU / mL, and store it in a 4°C refrigerator for standby use (the entire process of inoculation and dilution is carried out on a clean bench). Take 0.2mL of the bacterial suspension and spread it on the agar medium, dilute Examples 1-5 and Comparative Example 1 with deionized water to a 0.25g / mL liquid, soak the filter paper (diameter d0=6mm) in different liquids, stick it on the surface of the plate, soak the filter paper with sterile water as a blank control, repeat 4 filter paper pieces in each group, culture at 36°C for 24h, and measure the diameter (d) of the inhibition zone.
[0068] The experimental results are shown in Table 3.
[0069] Table 3 Statistical table of antibacterial zone diameter
[0070] sample Diameter(mm) Example 1 1.19±0.15 Example 2 1.80±0.06 Example 3 1.87±0.11 Example 4 1.84±0.15 Example 5 1.76±0.05 Comparative Example 1 1.17±0.07
[0071] As can be seen from Table 3, the whitening and brightening composition obtained by the present invention has excellent antibacterial effect. In addition, Examples 2-4 further enhance the antibacterial effect by treating Bamboo Striata, which proves that Bamboo Striata has a synergistic effect on the antibacterial effect of Sophora flavescens root extract.
[0072] The above embodiments and descriptions are only for illustrating the principles and best embodiments of the present invention. Without departing from the spirit and scope of the present invention, the present invention may be subject to various changes and improvements, all of which fall within the scope of the present invention to be protected.
Claims
1. A whitening and brightening composition, characterized in that: The invention comprises the following raw materials in parts by weight: 5-8 parts of Sophora flavescens root extract, 5-8 parts of Osmanthus fragrans extract, 3-5 parts of Pyracantha fortuneana fruit extract, 3-5 parts of Lycium barbarum fruit extract and 20-30 parts of niacinamide.
2. The whitening and brightening composition according to claim 1, characterized in that: The preparation method of the Osmanthus fragrans extract comprises the following steps: Roasting, water extraction and drying.
3. The whitening and brightening composition according to claim 2, characterized in that: The baking comprises: heating the mature main stem of the bamboo to 205-215° C. under anaerobic conditions for baking, raising the temperature to 220° C. within 10 seconds after a popping sound is heard from the mature main stem of the bamboo, keeping the temperature for 5-9 minutes, and then quickly taking it out and placing it in a room temperature environment until it cools to room temperature, thereby obtaining a baked bamboo.
4. The whitening and brightening composition according to claim 3, characterized in that: The water extraction comprises: adding water to roasted cinnamon bamboo in a weight ratio of 1:13 to break the wall and crush, then adding cellulase according to 5% of the weight of the roasted cinnamon bamboo, performing enzymolysis for 30-45 minutes under stirring to obtain enzymolysis solution, inactivating the enzyme in the enzymolysis solution, filtering and obtaining the supernatant to obtain cinnamon bamboo liquid.
5. The whitening and brightening composition according to claim 4, characterized in that: The enzyme inactivation method is: heating the enzymatic solution to 80° C. and maintaining it for 1 minute.
6. The whitening and brightening composition according to claim 5, characterized in that: The drying comprises: drying the bamboo liquid into powder to obtain the bamboo extract.
7. The whitening and brightening composition according to claim 6, characterized in that: The powder had a moisture content of 9%.
8. A method for preparing the whitening and brightening composition according to claim 1, characterized in that: The following steps are involved: Step 1, weighing the raw materials; Step 2, adding n-hexane to pulp the Sophora flavescens root extract, Osmanthus fragrans extract, Pyracantha fortuneana fruit extract and Lycium barbarum fruit extract in the raw materials at a weight of 3 times the sum of their weights; Step 3, degreasing the slurry; Step 4: Add niacinamide at room temperature and stir well to obtain a whitening and brightening composition.
9. The method for preparing the whitening and brightening composition according to claim 8, characterized in that: The degreasing process comprises: subjecting the slurry to ultrasonic treatment, with an ultrasonic frequency of 40000 Hz and a power of 60 W for 4 hours, and drying at 40-50° C. after filtering.
10. The method for preparing the whitening and brightening composition according to claim 8, characterized in that: The whitening and brightening composition is further added with 5.4-11.2 parts by weight of deionized water.