Protease supernatant extraction device capable of quickly isolating lower turbid liquid

Abstract

The utility model discloses a protease supernatant extraction device capable of quickly isolating lower turbid liquid, which comprises a centrifugal machine shell and an illuminating lamp, one side of the illuminating lamp is fixedly connected with one side of the centrifugal machine shell, and a centrifugal assembly for accelerating precipitation of protease is fixedly connected in the centrifugal machine shell. The right side of the centrifugal machine shell is fixedly connected with an adjusting and extracting assembly used for extracting protease clear liquid, and the adjusting and extracting assembly comprises a positioning block fixedly connected with one side of the centrifugal machine shell. According to the protease supernatant extraction device capable of rapidly isolating the lower-layer turbid liquid, by means of the arrangement of the adjusting extraction assembly, a limiting threaded groove plate is directly installed on the top of a centrifugal machine shell, then a threaded suction pipe is rotated, and a liquid extraction needle can be rapidly driven to move to the bottommost end of protease supernatant in a test tube; the operation precision is improved, the structure is simple, and expansion is convenient.

Description

Technical Field

[0001] The utility model relates to the technical field of extraction devices, in particular to a protease upper layer clear liquid extraction device which can quickly isolate a lower layer turbid liquid. Background Art

[0002] Protease is a general term for a class of enzymes that hydrolyze protein peptide chains.

[0003] The patent with announcement number CN218106891U discloses a protease upper layer clear liquid extraction device that can quickly isolate the lower layer turbid liquid, relates to the technical field of extraction devices, and mainly solves the problem that it is inconvenient to quickly remove and replace the filter when the extraction device extracts the protease clear liquid. The patent comprises an extraction tank, a liquid inlet is arranged on the right side of the extraction tank, and a liquid outlet is arranged on the left side of the extraction tank, a straw is arranged on the upper end of the extraction tank, and a filter is arranged inside the lower end of the extraction tank, and a quick replacement mechanism is arranged at both ends of the filter, and the quick replacement mechanism includes a baffle, a latch unit, a clamping unit, and an elastic unit, the lower sides of both ends of the filter are fitted with the baffle, and the side of the baffle close to the extraction tank is fixedly connected to the extraction tank, and by setting the quick removal mechanism, the filter can be quickly disassembled and cleaned and replaced after being used for a period of time, thereby facilitating preventing the filter from being blocked and affecting the filtering effect, and effectively improving the protein liquid clear liquid extraction efficiency.

[0004] During the preparation of a common solution containing protease, after the lysis or stirring operation, the protease solution is directly precipitated, and then the supernatant is directly extracted. The above patent lacks effective acceleration treatment for the protease solution before precipitation, and the supernatant cannot be extracted quickly and accurately. Utility Model Content

[0005] In view of the deficiencies in the prior art, the utility model provides a protease upper clear liquid extraction device that can quickly isolate the lower turbid liquid, solving the problems of the above patents lacking effective accelerated treatment of the protease liquid before precipitation and failing to quickly and accurately extract the upper clear liquid.

[0006] To achieve the above purpose, the utility model is implemented through the following technical solutions: a protease upper layer clear liquid extraction device that can quickly isolate the lower layer turbid liquid, comprising a centrifuge casing and a lighting lamp, one side of the lighting lamp is fixedly connected to one side of the centrifuge casing, a centrifugal assembly for accelerating the precipitation of protease is fixedly connected inside the centrifuge casing, an adjusting extraction assembly for extracting protease clear liquid is fixedly connected to the right side of the centrifuge casing, the adjusting extraction assembly comprises a positioning block fixedly connected to one side of the centrifuge casing, a positioning groove is provided on the inner wall of the positioning block, a positioning column is movably connected to the inner wall of the positioning groove, a limiting threaded groove plate is fixedly connected to the top of the positioning column, a threaded pipette is threadedly connected to one side of the limiting threaded groove plate, a piston plate is movably connected to the inner wall of the threaded pipette, a handle is fixedly connected to the middle of the top of the piston plate, a liquid extraction needle is fixedly connected to the bottom end of the threaded pipette, and a sealing cover is rotatably connected to the back side of the centrifuge casing through a damping shaft.

[0007] Preferably, the centrifugal assembly comprises a centrifugal motor fixedly connected to the middle of the inner wall of the centrifuge housing, and a load-bearing round block is fixedly connected to the top end of the output shaft of the centrifugal motor.

[0008] Preferably, a placement circular groove is provided on the surface of the bearing circular block, and anti-slip protrusions are provided on the inner surface of the placement circular groove.

[0009] Preferably, a connecting annular groove is provided on the surface of the top of the bearing round block, and an embedded ring plate is movably connected to the inner wall of the connecting annular groove.

[0010] Preferably, a non-slip pad is fixedly connected to the surface of the embedded ring plate, and a closing cover is fixedly connected to the top of the embedded ring plate.

[0011] Preferably, a cold air nozzle is fixedly connected to one side of the sealing cover, and an air inlet is fixedly connected to the other side of the sealing cover.

[0012] Beneficial Effects

[0013] The utility model provides a protease supernatant extraction device that can quickly isolate the lower turbid liquid. Compared with the prior art, it has the following beneficial effects:

[0014] (1) The device for extracting the supernatant liquid of the protease that can quickly isolate the turbid liquid of the lower layer is fixedly connected to the right side of the centrifuge housing with an adjusting and extracting component for extracting the supernatant liquid of the protease. The adjusting and extracting component includes a positioning block fixedly connected to one side of the centrifuge housing, a positioning groove is formed on the inner wall of the positioning block, a positioning column is movably connected to the inner wall of the positioning groove, a limiting thread groove plate is fixedly connected to the top of the positioning column, a threaded pipette is threadedly connected to one side of the limiting thread groove plate, a piston plate is movably connected to the inner wall of the threaded pipette, a handle is fixedly connected to the middle of the top of the piston plate, and a liquid extraction needle is fixedly connected to the bottom end of the threaded pipette. By using the setting of the adjusting and extracting component, the limiting thread groove plate is directly installed on the top of the centrifuge housing, and then the threaded pipette is rotated to quickly drive the liquid extraction needle to move to the bottom of the supernatant liquid of the protease in the test tube. Due to the use of thread adjustment, the operation accuracy is improved, and the structure is relatively simple, which is convenient for expansion.

[0015] (2) The protease upper layer clear liquid extraction device capable of quickly isolating the lower layer turbid liquid comprises a centrifugal motor fixedly connected to the middle of the inner wall of the centrifuge housing through a centrifugal assembly, a bearing round block fixedly connected to the top of the output shaft of the centrifugal motor, a placement round groove is provided on the surface of the bearing round block, and anti-skid protrusions are provided on the inner surface of the placement round groove, a connecting annular groove is provided on the surface of the top of the bearing round block, an embedded ring plate is movably connected to the inner wall of the connecting annular groove, an anti-skid pad is fixedly connected to the surface of the embedded ring plate, a sealing cover is fixedly connected to the top of the embedded ring plate, a cold air nozzle is fixedly connected to one side of the sealing cover, and an air inlet is fixedly connected to the other side of the sealing cover. Through the arrangement of the centrifugal assembly, the cold air nozzle and the air inlet, when the device rotates at a high speed, the test tube is first directly clamped and limited by the sealing cover, and then limited by the sealing cover. The sealing cover is easy to install, thereby improving the reliability of the centrifugal operation. By adding cold air to the centrifugal process, the sedimentation time will be greatly shortened, which is convenient for subsequent rapid isolation of the turbid liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a three-dimensional schematic diagram of the utility model;

[0017] Figure 2 For the utility model Figure 1 A partial enlarged view of the middle A;

[0018] Figure 3 This is a schematic diagram of the installation of the closed cover structure of the utility model;

[0019] Figure 4 It is a three-dimensional schematic diagram of the sealing cover structure of the utility model;

[0020] Figure 5 It is a schematic diagram of the structure of the adjustment and extraction component of the utility model;

[0021] Figure 6It is a cross-sectional view of the centrifugal assembly and the regulating and extracting assembly structure of the utility model;

[0022] Figure 7 For this utility model Figure 6 A partial enlarged view of point B in the middle.

[0023] In the figure: 1. Centrifuge casing; 2. Lighting lamp; 3. Centrifugal assembly; 31. Centrifugal motor; 32. Carrying round block; 33. Placement round groove; 34. Connecting annular groove; 35. Embedded ring plate; 36. Anti-skid pad; 37. Closing cover; 4. Adjustment and extraction assembly; 41. Positioning block; 42. Positioning groove; 43. Positioning column; 44. Limiting threaded groove plate; 45. Threaded suction tube; 46. Piston plate; 47. Handle; 48. Extraction needle; 5. Sealing cover; 6. Air cooling nozzle; 7. Air inlet. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] See also Figure 1-7 , the utility model provides two technical solutions:

[0026] Embodiment 1, a device for extracting a supernatant liquid of a protease that can quickly isolate a turbid liquid in a lower layer, comprises a centrifuge casing 1, an illuminating lamp 2, one side of the illuminating lamp 2 is fixedly connected to one side of the centrifuge casing 1, a centrifugal assembly 3 for accelerating the precipitation of the protease is fixedly connected inside the centrifuge casing 1, a regulating extraction assembly 4 for extracting the protease supernatant is fixedly connected to the right side of the centrifuge casing 1, the regulating extraction assembly 4 comprises a positioning block 41 fixedly connected to one side of the centrifuge casing 1, a positioning groove 42 is provided on the inner wall of the positioning block 41, a positioning column 43 is movably connected to the inner wall of the positioning groove 42, the top of the positioning column 43 is fixedly connected to a limiting threaded groove plate 44, and one side of the limiting threaded groove plate 44 is threadedly connected to a threaded The outer surface of the threaded straw 45 is provided with threads, which are adapted to the inner wall of the limiting thread groove plate 44. The inner wall of the threaded straw 45 is movably connected with a piston plate 46, and the middle of the top of the piston plate 46 is fixedly connected with a handle 47. The bottom end of the threaded straw 45 is fixedly connected with a liquid extraction needle 48. The back of the centrifuge housing 1 is rotatably connected with a sealing cover 5 through a damping shaft. By adjusting the setting of the extraction component 4, the limiting thread groove plate 44 is directly installed on the top of the centrifuge housing 1, and then the threaded straw 45 is rotated to quickly drive the liquid extraction needle 48 to move to the bottom of the upper clear liquid of the protease in the test tube. Due to the use of thread adjustment, the operating accuracy is improved, and the structure is relatively simple, which is convenient for expansion.

[0027] Embodiment 2, which is different from Embodiment 1 mainly in that: a protease supernatant extraction device capable of quickly isolating the lower turbid liquid, wherein the centrifugal assembly 3 comprises a centrifugal motor 31 fixedly connected to the middle of the inner wall of the centrifuge housing 1, a bearing block 32 fixedly connected to the top of the output shaft of the centrifugal motor 31, a placement circular groove 33 is provided on the surface of the bearing block 32, an anti-slip convex point is provided on the inner surface of the placement circular groove 33, a connecting annular groove 34 is provided on the surface of the top of the bearing block 32, an embedded ring plate 35 is movably connected to the inner wall of the connecting annular groove 34, and the surface of the embedded ring plate 35 is fixedly connected to the inner wall of the connecting annular groove 34. A non-slip pad 36 is fixedly connected, a closing cover 37 is fixedly connected to the top of the embedded ring plate 35, a cooling nozzle 6 is fixedly connected to one side of the sealing cover 5, and an air inlet 7 is fixedly connected to the other side of the sealing cover 5. Through the arrangement of the centrifugal assembly 3, the cooling nozzle 6 and the air inlet 7, when the device rotates at a high speed, the test tube is first clamped and limited by the closing cover 37 directly, and then limited by the sealing cover 5. The closing cover 37 is easy to install, which improves the reliability of the centrifugal operation. By adding cooling air to the centrifugal process, the sedimentation time will be greatly shortened, which is convenient for the subsequent rapid isolation of turbid liquid.

[0028] The present application directly shortens the precipitation time by superimposing cooling precipitation with centrifugal precipitation. The added cold air can be various low-temperature inert gases such as liquid nitrogen. The height of the closing cover 37 will not affect the closure of the sealing cover 5. The threaded straw 45 and the related overall structure are similar to a needle tube.

[0029] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0030] During operation, the test tube containing the protease is directly placed into the connecting annular groove 34, and then the embedded ring plate 35 is inserted into the placement groove 33 on the inner wall of the supporting round block 32, and the anti-skid pad 36 is inserted into the placement groove 33 to increase the static friction. Then the sealing cover 5 is closed, the external cold source is connected through the air inlet 7, cold air is introduced, and the centrifugal motor 31 is turned on to centrifuge the protease in a low-temperature environment. After the centrifugation is completed, the positioning column 43 is inserted into the positioning groove 42, and the threaded pipette 45 is rotated so that the liquid extraction needle 48 is inserted into the test tube, and the bottom end of the liquid extraction needle 48 is closest to the bottom end of the upper clear liquid. Then the handle 47 is pulled up to drive the piston plate 46 to move upward to extract the clear liquid, and then the supporting round block 32 is rotated to operate the test tubes one by one. After the work is completed, the device is restored.

[0031] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0032] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for extracting supernatant liquid of a protease layer which can quickly isolate the turbid liquid layer below, comprising a centrifuge housing (1) and an illuminating lamp (2), wherein one side of the illuminating lamp (2) is fixedly connected to one side of the centrifuge housing (1). Features: A centrifugal assembly (3) for accelerating the precipitation of proteases is fixedly connected inside the centrifuge housing (1). A regulating and extracting assembly (4) for extracting protease clear liquid is fixedly connected to the right side of the centrifuge housing (1). The regulating and extracting assembly (4) comprises a positioning block (41) fixedly connected to one side of the centrifuge housing (1). A positioning groove (42) is provided on the inner wall of the positioning block (41). A positioning column (43) is movably connected to the inner wall of the positioning groove (42). A limiting thread groove plate (44) is fixedly connected to the top of the positioning column (43). A threaded pipette (45) is threadedly connected to one side of the limiting thread groove plate (44). A piston plate (46) is movably connected to the inner wall of the threaded pipette (45). A handle (47) is fixedly connected to the middle of the top of the piston plate (46). A liquid extraction needle (48) is fixedly connected to the bottom end of the threaded pipette (45). A sealing cover (5) is rotatably connected to the back side of the centrifuge housing (1) via a damping shaft.

2. A protease supernatant extraction device capable of quickly isolating the lower turbid liquid according to claim 1, Features: The centrifugal assembly (3) comprises a centrifugal motor (31) fixedly connected to the middle of the inner wall of the centrifuge housing (1), and a bearing round block (32) is fixedly connected to the top end of the output shaft of the centrifugal motor (31).

3. A protease supernatant extraction device capable of quickly isolating the lower turbid liquid according to claim 2, Features: A placement circular groove (33) is provided on the surface of the bearing circular block (32), and anti-slip convex points are provided on the inner surface of the placement circular groove (33).

4. A protease supernatant extraction device capable of quickly isolating the lower turbid liquid according to claim 2, Features: A connecting annular groove (34) is provided on the surface of the top of the bearing round block (32), and an embedded ring plate (35) is movably connected to the inner wall of the connecting annular groove (34).

5. A protease supernatant extraction device capable of quickly isolating the lower turbid liquid according to claim 4, Features: An anti-slip pad (36) is fixedly connected to the surface of the embedded ring plate (35), and a closing cover (37) is fixedly connected to the top of the embedded ring plate (35).

6. A protease supernatant extraction device capable of quickly isolating the lower turbid liquid according to claim 1, Features: A cold air nozzle (6) is fixedly connected to one side of the sealing cover (5), and an air inlet (7) is fixedly connected to the other side of the sealing cover (5).

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