Pfu replication accessory factors and methods of use

a technology of accessory factors and pfu, which is applied in the field of replication, amplifying and sequencing nucleic acids, can solve the problems of taq polymerase fundamental, pcr has limitations, and dna template exposure amplification, and achieve the effect of enhancing the activity of polymerases
US20090221029A1Inactive Publication Date: 2009-09-03STRATAGENE INC US
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
STRATAGENE INC US
Publication Date
2009-09-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

This invention provides isolated polynucleotides that encode replication accessory factors. The invention also provides novel DNA replication accessory factors, which have been isolated and purified from the hyperthermophilic archaea Pyrococcus furiosus. The invention also provides various methods of enhancing a nucleic acid polymerase reaction comprising the addition of the replication accessory factors to the reaction. This invention further provides methods of synthesizing, amplifying, and mutagenizing nucleic acids of interest employing the replication accessory factors. This invention also provides kits comprising, at least one of the replication accessory factors. This invention also provides kits useful for various methods that comprise at least one replication accessory factor.
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Description

RELATED APPLICATION INFORMATION

[0001] This application is a divisional application of U.S. application Ser. No. 10 / 828,924, filed Apr. 20, 2004 (now U.S. Pat. No. 7,442,766), which is a continuation of U.S. application Ser. No. 09 / 626,813, filed Jul. 27, 2000 (abandoned), which claims the benefit of U.S. Provisional Application Ser. No. 60 / 146,580, filed Jul. 30, 1999. Each of these prior application is herein incorporated by reference in its entirety.BACKGROUND AND SUMMARY OF THE INVENTION

[0002] The invention relates to the field of replicating, amplifying, and sequencing nucleic acids. Further, this invention relates to novel proteins that enhance the activity of the polymerases.

[0003] In vitro polymerization techniques have enormously benefited the fields of biotechnology and medicine. The ability to manipulate nucleic acids with polymerization reactions greatly facilitates techniques ranging from gene characterization and molecular cloning (including, but not limited to sequencing,...

Claims

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