Methods for cannabinoid quantification

a cannabinoid and quantification method technology, applied in the field of cannabinoid quantification, can solve the problems of limited quantification resolution of the test, the test is still quite complex for the average untrained user, etc., and achieve the effect of expanding the range and accuracy of quantification

Inactive Publication Date: 2017-08-17
COMPASSIONATE ANALYTICS
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention relates to a method for visualizing cannabinoids in a liquid solution or on a test paper. The method involves contacting the cannabinoids with one or more cannabinoid-sensitive visualization reagents in a separate reaction vessel or on a test strip / kits. The visualization reaction can occur in a liquid solution or on a test paper. The use of multiple visualization reagents with different quantification ranges and sensitivities allows for a more accurate and extended range of quantification. The activity of the visualization reagent is retained when the test strip is used by the end-user.

Problems solved by technology

Quantification of cannabinoids in a sample generally requires a laboratory test, for instance using gas chromatography, which can be expensive and generally requires time to send the sample to an appropriate lab and wait for the results.
Home-based tests, such as Alpha-Cat, have been developed using thin-layer chromatography, however this test is still quite complex for the average untrained user, and the quantification resolution of the test is limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Methods for cannabinoid quantification
  • Methods for cannabinoid quantification
  • Methods for cannabinoid quantification

Examples

Experimental program
Comparison scheme
Effect test

example 1

ation of THC Content of an Unknown Solid Cannabis Sample Using the Compositions and Methods of the Invention—Visualization in Solution

[0070]Preparation of Cannabinoid-Sensitive Visualization Reagents

[0071]Fast Blue BB diazonium salt was dissolved in methanol to a final concentration of 0.1%.

[0072]Production of Calibrated Quantification Reference Chart

[0073]Solid cannabis samples with known THC concentration were used to create the calibrated quantification reference chart. These cannabis samples were known to have negligible concentrations of other cannabinoids that might also react with the cannabinoid-sensitive visualization reagent, such as CBD or CBN. For each sample, the following protocol was followed to extract the THC into a liquid THC containing solution. The solid sample was macerated into small pieces, and a portion was wrapped in aluminium foil. This was heated at 325° F. for exactly 5 minutes. The resulting solid sample was removed from the foil, crumbled, and a 0.1 g p...

example 2

ation of THC Content of an Unknown Solid Cannabis Sample Using an Extended Range Calibrated Quantification Reference Chart—Visualization in Solution

A. Preparation of Two Cannabinoid-Sensitive Visualization Reagents

[0075]Fast Blue BB salt was dissolved in methanol to a final concentration of 0.1%. Fast Corinth V was dissolved in methanol to a final concentration of 0.1%

B. Production of Calibrated Quantification Reference Chart

[0076]Solid cannabis samples with known THC concentration were used to create the calibrated quantification reference chart. These cannabis samples were known to have negligible concentrations of other cannabinoids that might also react with the cannabinoid-sensitive visualization reagent, such as CBD or cannabinol. For each sample, the following protocol was followed to extract the THC into a liquid THC containing solution. The solid sample was macerated into small pieces, and a portion was wrapped in aluminium foil. This was heated at 325 degrees Celsius for e...

example 3

ation of CBD Content of an Unknown Solid Cannabis Sample Using the Compositions and Methods of the Invention—Visualization in Solution

A. Preparation of Cannabinoid-Sensitive Visualization Reagent

[0078]Potassium hydroxide was dissolved in water to a final concentration of 20%.

B. Production of Calibrated Quantification Reference Chart

[0079]Solid cannabis samples with known CBD concentration were used to create the calibrated quantification reference chart. For each sample, the following protocol was followed to extract the CBD into a liquid CBD containing solution. The solid sample was macerated into small pieces, and a portion was wrapped in aluminium foil. This was heated at 325 degrees Celsius for exactly 5 minutes. The resulting solid sample was removed from the foil, crumbed, and a 0.1 g portion was placed into a plastic container. Exactly 3.0 mL of methanol or isopropanol was placed into the plastic container. The container with the 0.1 g solid sample and 3.0 mL of methanol or i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
color changeaaaaaaaaaa
coloraaaaaaaaaa
Login to View More

Abstract

A method for quantification of the concentration of one or more cannabinoid compounds in a liquid sample is provided. The method involves contacting the liquid sample with at least one cannabinoid-sensitive visualization reagent, allowing the at least one cannabinoid-sensitive visualization reagent to develop for a defined amount of time; and comparing the resulting color change of the at least one cannabinoid-sensitive visualization reagent to a calibrated quantification reference chart.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation application of U.S. Non-Provisional application Ser. No. 14 / 771,592, filed Aug. 31, 2015, entitled “Methods for Cannabinoid Quantification”, which is a U.S. National Stage Entry Application of International Application Serial Number PCT / CA2014 / 000157, filed Feb. 28, 2014, entitled “Methods for Cannabinoid Quantification”, which claims priority to U.S. Provisional Patent Application Ser. No. 61 / 771,263, filed Mar. 1, 2013, entitled “Cannabinoid Quantification”, U.S. Provisional Patent Application Ser. No. 61 / 827,128, filed May 24, 2013 entitled “Cannabinoid Quantification, In Solution” and, U.S. Provisional Patent Application Ser. No. 61 / 884,409, filed Sep. 30, 2013, entitled “Cannabinoid Quantification,” all of which are hereby incorporated by reference for all purposes.FIELD OF THE INVENTION[0002]The present invention relates to compositions and methods for accurate quantification of cannabinoid compoun...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityApplications(United States)
IPC IPC(8): G01N33/94G01N33/52
CPCG01N33/523G01N33/948G01N33/52G01N33/5302G01N21/78
InventorLUCAS, PHILIPPEEADES, CALEB
OwnerCOMPASSIONATE ANALYTICS