Methods for cannabinoid quantification

a cannabinoid and quantification method technology, applied in the field of cannabinoid quantification, can solve the problems of limited quantification resolution of the test, the test is still quite complex for the average untrained user, etc., and achieve the effect of expanding the range and accuracy of quantification

Inactive Publication Date: 2016-01-21
COMPASSIONATE ANALYTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention provides a variety of methods for visualizing cannabinoids in liquid or solid samples. The method involves contacting the sample with one or more cannabinoid-sensitive visualization reagents in solution or on a test paper or strip. The use of multiple reagents with different sensitivities allows for an extended range and accuracy of quantification. The activity of the reagent is retained when the test is used by the end-user. These methods have practical applications in drug testing and forensics.

Problems solved by technology

Quantification of cannabinoids in a sample generally requires a laboratory test, for instance using gas chromatography, which can be expensive and generally requires time to send the sample to an appropriate lab and wait for the results.
Home-based tests, such as Alpha-Cat, have been developed using thin-layer chromatography, however this test is still quite complex for the average untrained user, and the quantification resolution of the test is limited.

Method used

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  • Methods for cannabinoid quantification
  • Methods for cannabinoid quantification
  • Methods for cannabinoid quantification

Examples

Experimental program
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Effect test

example 1

ation of THC Content of an Unknown Solid Cannabis Sample Using the Compositions and Methods of the Invention—Visualization in Solution

[0069]A. Preparation of Cannabinoid-Sensitive Visualization Reagents

[0070]Fast Blue BB diazonium salt was dissolved in methanol to a final concentration of 0.1%.

[0071]B. Production of Calibrated Quantification Reference Chart

[0072]Solid cannabis samples with known THC concentration were used to create the calibrated quantification reference chart. These cannabis samples were known to have negligible concentrations of other cannabinoids that might also react with the cannabinoid-sensitive visualization reagent, such as CBD or CBN. For each sample, the following protocol was followed to extract the THC into a liquid THC containing solution. The solid sample was macerated into small pieces, and a portion was wrapped in aluminum foil. This was heated at 325° F. for exactly 5 minutes. The resulting solid sample was removed from the foil, crumbled, and a 0....

example 2

ation of THC Content of an Unknown Solid Cannabis Sample Using an Extended Range Calibrated Quantification Reference Chart-Visualization in Solution

[0075]A. Preparation of Two Cannabinoid-Sensitive Visualization Reagents

[0076]Fast Blue BB salt was dissolved in methanol to a final concentration of 0.1%. Fast Corinth V was dissolved in methanol to a final concentration of 0.1%

[0077]B. Production of Calibrated Quantification Reference Chart

[0078]Solid cannabis samples with known THC concentration were used to create the calibrated quantification reference chart. These cannabis samples were known to have negligible concentrations of other cannabinoids that might also react with the cannabinoid-sensitive visualization reagent, such as CBD or cannabinol. For each sample, the following protocol was followed to extract the THC into a liquid THC containing solution. The solid sample was macerated into small pieces, and a portion was wrapped in aluminum foil. This was heated at 325 degrees Ce...

example 3

ation of CBD Content of an Unknown Solid Cannabis Sample Using the Compositions and Methods of the Invention-Visualization in Solution

[0081]A. Preparation of Cannabinoid-Sensitive Visualization Reagent

[0082]Potassium hydroxide was dissolved in water to a final concentration of 20%.

[0083]B. Production of Calibrated Quantification Reference Chart

[0084]Solid cannabis samples with known CBD concentration were used to create the calibrated quantification reference chart. For each sample, the following protocol was followed to extract the CBD into a liquid CBD containing solution. The solid sample was macerated into small pieces, and a portion was wrapped in aluminum foil. This was heated at 325 degrees Celsius for exactly 5 minutes. The resulting solid sample was removed from the foil, crumbed, and a 0.1 g portion was placed into a plastic container. Exactly 3.0 mL of methanol or isopropanol was placed into the plastic container. The container with the 0.1 g solid sample and 3.0 mL of me...

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Abstract

A method for quantification of the concentration of one or more cannabinoid compounds in a liquid sample is provided. The method involves contacting the liquid sample with at least one cannabinoid-sensitive visualization reagent, allowing the at least one cannabinoid-sensitive visualization reagent to develop for a defined amount of time; and comparing the resulting color change of the at least one cannabinoid-sensitive visualization reagent to a calibrated quantification reference chart.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority from U.S. Provisional Patent Application Ser. No. 61 / 771,263 filed on Mar. 1, 2013, entitled “Cannabidiol Quantification”; U.S. Provisional Patent Application Ser. No. 61 / 827,128 filed on May 24, 2013 entitled “Cannabinoid Quantification, In Solution”; and U.S. Provisional Patent Application Ser. No. 61 / 884,409 filed on Sep. 30, 2013 entitled “Cannabinoid Quantification”, all of which are expressly incorporated by reference herein to the extent permitted by law.FIELD OF THE INVENTION[0002]The present invention relates to compositions and methods for accurate quantification of cannabinoid compounds in a sample.BACKGROUND OF THE INVENTION[0003]The legal production, sale and use of cannabis for medical purposes is becoming more prevalent in many countries, including the United States and Canada. It is important for medical cannabis growers, dispensaries and end-users to know the concentration of certain canna...

Claims

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Application Information

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IPC IPC(8): G01N33/94
CPCG01N33/948G01N33/52G01N33/5302G01N21/78G01N33/523
InventorLUCAS, PHILIPPEEADES, CALEB
OwnerCOMPASSIONATE ANALYTICS