Dogwood fruit extract and its preparing method and use

Abstract

The present invention discloses dogwood fruit extract and its preparation process as well as the new use of medicine containing the extract. The extract contains morroniside in 25-50 % and loganin in 25-40 %. It is used in preparing medicine for treating ischemic vascular diseases, including vascular convulsion, thrombosis, vessel obstruction, local organ circulation or function obstruction caused by disease and damage of artery and vein system. Clinically, the treated diseases include arteriosclerosis, horary ischemia, tissue and organ obstruction, peripheral blood vessel ischemia, etc. The present invention also relates to the medicine preparation containing morroniside and loganin for treating ischemic vascular diseases.

Description

technical field

[0001] The invention provides a cornus officinalis extract, and also relates to a preparation method of the extract and an application of the extract, belonging to the field of traditional Chinese medicine. Background technique

[0002] Ischemic vascular disease mainly refers to vasospasm, thrombosis or vascular occlusion caused by damage to the arteriovenous system, resulting in local circulation and dysfunction of organs, mainly including arteriosclerosis, transient ischemic attack, infarction of various tissues and organs and Peripheral vascular occlusion, etc. Ischemic vascular disease is a common and frequently-occurring disease that endangers human life and health. It has the characteristics of high morbidity, disability, mortality and recurrence. It is the main cause of death and disability in middle-aged and elderly people. Therefore, it is of great significance to discover and develop drugs for the prevention and treatment of ischemic vascular disea...

Examples

Embodiment 1

[0108] Example 1: Preparation of Cornus officinalis Extract

[0109] Cornus officinalis medicinal materials are decocted 3 times with water, and the amount of water is added 6 times, and the time is 2 hours. Combine the decoctions for 3 times, filter, concentrate under reduced pressure, and refrigerate for later use after filtration; extract the concentrated Cornus officinalis water, put it on a HP20 macroporous adsorption resin column, let the liquid medicine flow through the whole column, and use deionized water, 30%, Carry out gradient elution with 50% ethanol, collect the eluate of 50% ethanol, and concentrate under reduced pressure to form an extract; extract the extract obtained by separating the macroporous adsorption resin with ethanol, remove the organic solvent, concentrate, and dry to obtain a solid product . Yield 2.5%.

Embodiment 2

[0110] Example 2: Preparation of Cornus officinalis Extract

[0111] Cornus officinalis medicinal materials are decocted 3 times with water, and the amount of water is added 6 times, and the time is 2 hours. Combine the decoctions for 3 times, filter, concentrate under reduced pressure, add ethanol to adjust the alcohol concentration to 70%, refrigerate for 24 hours, filter, recover ethanol from the filtrate under reduced pressure, and refrigerate for later use.

[0112] Take the above-mentioned water extraction and alcohol precipitation extract of Cornus officinalis, put it on a HP20 macroporous adsorption resin column, let the medicinal solution flow through the whole column, and carry out gradient elution with deionized water, 30% and 50% ethanol in sequence, and collect the elution of 50% ethanol. liquid, concentrated under reduced pressure into an extract.

[0113] On Al 2 o 3 Chromatographic column, eluted with ethanol for purification, and freeze-dried to obtain a ...

Embodiment 3

[0115] Example 3: Capsules

[0116] The cornus officinalis extract 300g of the present invention 1000g of medicinal starch, mix evenly, pack into No. 1 capsule, each 0.35g, take 1-2 capsules orally each time, twice a day.

technical field

[0001] The invention provides a cornus officinalis extract, and also relates to a preparation method of the extract and an application of the extract, belonging to the field of traditional Chinese medicine. Background technique

[0002] Ischemic vascular disease mainly refers to vasospasm, thrombosis or vascular occlusion caused by damage to the arteriovenous system, resulting in local circulation and dysfunction of organs, mainly including arteriosclerosis, transient ischemic attack, infarction of various tissues and organs and Peripheral vascular occlusion, etc. Ischemic vascular disease is a common and frequently-occurring disease that endangers human life and health. It has the characteristics of high morbidity, disability, mortality and recurrence. It is the main cause of death and disability in middle-aged and elderly people. Therefore, it is of great significance to discover and develop drugs for the prevention and treatment of ischemic vascular disea...

Examples

Embodiment 1

[0108] Example 1: Preparation of Cornus officinalis Extract

[0109] Cornus officinalis medicinal materials are decocted 3 times with water, and the amount of water is added 6 times, and the time is 2 hours. Combine the decoctions for 3 times, filter, concentrate under reduced pressure, and refrigerate for later use after filtration; extract the concentrated Cornus officinalis water, put it on a HP20 macroporous adsorption resin column, let the liquid medicine flow through the whole column, and use deionized water, 30%, Carry out gradient elution with 50% ethanol, collect the eluate of 50% ethanol, and concentrate under reduced pressure to form an extract; extract the extract obtained by separating the macroporous adsorption resin with ethanol, remove the organic solvent, concentrate, and dry to obtain a solid product . Yield 2.5%.

Embodiment 2

[0110] Example 2: Preparation of Cornus officinalis Extract

[0111] Cornus officinalis medicinal materials are decocted 3 times with water, and the amount of water is added 6 times, and the time is 2 hours. Combine the decoctions for 3 times, filter, concentrate under reduced pressure, add ethanol to adjust the alcohol concentration to 70%, refrigerate for 24 hours, filter, recover ethanol from the filtrate under reduced pressure, and refrigerate for later use.

[0112] Take the above-mentioned water extraction and alcohol precipitation extract of Cornus officinalis, put it on a HP20 macroporous adsorption resin column, let the medicinal solution flow through the whole column, and carry out gradient elution with deionized water, 30% and 50% ethanol in sequence, and collect the elution of 50% ethanol. liquid, concentrated under reduced pressure into an extract.

[0113] On Al 2 o 3 Chromatographic column, eluted with ethanol for purification, and freeze-dried to obtain a ...

Embodiment 3

[0115] Example 3: Capsules

[0116] The cornus officinalis extract 300g of the present invention 1000g of medicinal starch, mix evenly, pack into No. 1 capsule, each 0.35g, take 1-2 capsules orally each time, twice a day.