Celery seed ethyl acetate extract and use thereof

Abstract

The invention disclosed the application of celery seed coumarins and flavanoid in preparing arthrifuge, anti-inflammatory agent or health food, which belongs to the natural occurring drugs and medical technology domain. The coumarins and flavanoid is the extract from acetic ether. The preparing method includes the following steps: selecting 10kg celery dry seed, adding 15-20 times of 75-95%(V / V) as the seed or extracting with 4-5 times of 75-95%(V / V) alcohol at higher temperature, heating for 1-1.5h every time; vacuum condensing the extracts until nearly all the alcohol is away; adding 1-2 times of water, extracting with 1 / 3 volume of the diluted solution sherwood oil (60-90DEG C) for 2-3 times; reclaiming the sherwood oil; extracting the water layer with 1 / 3 volume of acetic ether for 4 times; reclaiming the acetic ether to get the extracts. The invention has very simple preparing technologies; the product in the invention has obvious anti-inflammatory function; it can also decrease the amount of serum uric acid of rats.

Description

technical field

[0001] The present invention relates to the use of celery seed flavonoids and coumarins in the preparation of medicines for preventing and treating gout. The application in medicine, anti-inflammatory medicine or health food belongs to the field of natural medicinal chemistry and medical technology. Background technique

[0002] Celery seed is the seed of celery Apium graveolens L., an umbrella family plant. It has a history of 100 years in Australia to treat joint pain. It is regarded as a traditional folk remedy, and celery seed extract is taken to relieve joint pain. Modern nutrition preliminarily affirms that celery seed oil contains nutrients that are beneficial to articular cartilage, but there is no strong evidence to prove it. The chemical components reported by foreign scholars are mainly umbelliferin and apigenin [Grag, S.K.; Gupta, S.R.; Sharma, N.D. Apiumoside, a new furanocoumarin glucoside from the seeds of Apiumgraveolens. Phytochemistry 1979 ...

Examples

Embodiment 1

[0046] Embodiment 1 preparation method of celery seed flavonoids and coumarin compounds

[0047] 10 kg of dried celery seeds were extracted by percolation with 95% (V / V) ethanol, and 15 times the amount of percolation liquid was collected, and the extract was concentrated under reduced pressure at 55° C. to almost no ethanol to obtain a thick extract. Add 1 times the amount of water to the extract to dilute the suspension, and extract 3 times with one-third volume of petroleum ether (60-90°C) of the diluent, separate the petroleum ether and recover the petroleum ether extract of petroleum ether. The aqueous layer is extracted 4 times with one-third volume of ethyl acetate, separates ethyl acetate and reclaims ethyl acetate to obtain celery seed ethyl acetate extract, which mainly contains celery seed coumarin and flavonoids Compound components are represented by (CFE). The content of flavonoids in the extract is 55-65%, and the content of coumarins is 5-10%.

Embodiment 2

[0048] Embodiment 2 preparation method of celery seed flavonoids and coumarin compounds

[0049] 10 kg of dried celery seeds were extracted by percolation with 75% (V / V) ethanol, and 20 times the amount of percolation liquid was collected, and the extract was concentrated under reduced pressure at 55° C. to almost no ethanol to obtain a thick extract. Add 2 times the amount of water to the extract to dilute the suspension, then extract 2 times with 1 / 3 volume of petroleum ether (60-90°C) of the diluent, separate the petroleum ether and recover the petroleum ether extract of petroleum ether. The aqueous layer is extracted 4 times with one-third volume of ethyl acetate, separates ethyl acetate and reclaims ethyl acetate to obtain celery seed ethyl acetate extract, which mainly contains celery seed coumarin and flavonoids Compound components are represented by (CFE). The content of flavonoids in the extract is 55-65%, and the content of coumarins is 5-10%.

Embodiment 3

[0050] Embodiment 3 preparation method of celery seed flavonoids and coumarin compounds

[0051] 10 kg of dried celery seeds were heated and extracted with 4 times the amount of 95% (V / V) ethanol for 3 times, each heating for 1 hour; the extract was collected, concentrated under reduced pressure at 55°C until nearly free of ethanol, and a thick extract was obtained. Add 1 times the amount of water to the extract to dilute the suspension, and extract 3 times with one-third volume of petroleum ether (60-90°C) of the diluent, separate the petroleum ether and recover the petroleum ether extract of petroleum ether. The aqueous layer is extracted 4 times with one-third volume of ethyl acetate, separates ethyl acetate and reclaims ethyl acetate to obtain celery seed ethyl acetate extract, which mainly contains celery seed coumarin and flavonoids Compound components are represented by (CFE). The content of flavonoids in the extract is 55-65%, and the content of coumarins is 5-10%.

technical field

[0001] The present invention relates to the use of celery seed flavonoids and coumarins in the preparation of medicines for preventing and treating gout. The application in medicine, anti-inflammatory medicine or health food belongs to the field of natural medicinal chemistry and medical technology. Background technique

[0002] Celery seed is the seed of celery Apium graveolens L., an umbrella family plant. It has a history of 100 years in Australia to treat joint pain. It is regarded as a traditional folk remedy, and celery seed extract is taken to relieve joint pain. Modern nutrition preliminarily affirms that celery seed oil contains nutrients that are beneficial to articular cartilage, but there is no strong evidence to prove it. The chemical components reported by foreign scholars are mainly umbelliferin and apigenin [Grag, S.K.; Gupta, S.R.; Sharma, N.D. Apiumoside, a new furanocoumarin glucoside from the seeds of Apiumgraveolens. Phytochemistry 1979 ...

Examples

Embodiment 1

[0046] Embodiment 1 preparation method of celery seed flavonoids and coumarin compounds

[0047] 10 kg of dried celery seeds were extracted by percolation with 95% (V / V) ethanol, and 15 times the amount of percolation liquid was collected, and the extract was concentrated under reduced pressure at 55° C. to almost no ethanol to obtain a thick extract. Add 1 times the amount of water to the extract to dilute the suspension, and extract 3 times with one-third volume of petroleum ether (60-90°C) of the diluent, separate the petroleum ether and recover the petroleum ether extract of petroleum ether. The aqueous layer is extracted 4 times with one-third volume of ethyl acetate, separates ethyl acetate and reclaims ethyl acetate to obtain celery seed ethyl acetate extract, which mainly contains celery seed coumarin and flavonoids Compound components are represented by (CFE). The content of flavonoids in the extract is 55-65%, and the content of coumarins is 5-10%.

Embodiment 2

[0048] Embodiment 2 preparation method of celery seed flavonoids and coumarin compounds

[0049] 10 kg of dried celery seeds were extracted by percolation with 75% (V / V) ethanol, and 20 times the amount of percolation liquid was collected, and the extract was concentrated under reduced pressure at 55° C. to almost no ethanol to obtain a thick extract. Add 2 times the amount of water to the extract to dilute the suspension, then extract 2 times with 1 / 3 volume of petroleum ether (60-90°C) of the diluent, separate the petroleum ether and recover the petroleum ether extract of petroleum ether. The aqueous layer is extracted 4 times with one-third volume of ethyl acetate, separates ethyl acetate and reclaims ethyl acetate to obtain celery seed ethyl acetate extract, which mainly contains celery seed coumarin and flavonoids Compound components are represented by (CFE). The content of flavonoids in the extract is 55-65%, and the content of coumarins is 5-10%.

Embodiment 3

[0050] Embodiment 3 preparation method of celery seed flavonoids and coumarin compounds

[0051] 10 kg of dried celery seeds were heated and extracted with 4 times the amount of 95% (V / V) ethanol for 3 times, each heating for 1 hour; the extract was collected, concentrated under reduced pressure at 55°C until nearly free of ethanol, and a thick extract was obtained. Add 1 times the amount of water to the extract to dilute the suspension, and extract 3 times with one-third volume of petroleum ether (60-90°C) of the diluent, separate the petroleum ether and recover the petroleum ether extract of petroleum ether. The aqueous layer is extracted 4 times with one-third volume of ethyl acetate, separates ethyl acetate and reclaims ethyl acetate to obtain celery seed ethyl acetate extract, which mainly contains celery seed coumarin and flavonoids Compound components are represented by (CFE). The content of flavonoids in the extract is 55-65%, and the content of coumarins is 5-10%.

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