Method for producing energy ethanol by fermentation of waste paper pulp

Abstract

The present invention discloses a method for producing energy alcohol by utilizing waste paper pulp fermentation. The method at least includes the following steps: preparation of yeast starter liquor, preparation and sterilization of fermentation medium, synchronous diastatic fermentation and distillation for dehydration. In the step of synchronous diastatic fermentation, after fermentation lasts for 12 to 60 hours, waste paper pulp, corn steep liquor and enzyme are supplemented, so that the alcoholicity of yeast mash of 72 to 96 hours can reach over 40g / L, and fermentation is stopped. Containing fine-particle cellulose and hemicellulose, the waste paper pulp in the present invention, which is the waste of the papermaking industry, has a large specific surface area, which is favorable for enzymolysis and saccharification, so the waste paper pulp can be used for synchronous diastatic fermentation without being preprocessed, and moreover, the waste paper pulp is characterized by negative cost. Consequently, using waste paper pulp fermentation to produce the energy alcohol not only can leave out materials and preprocessing expense, the cost of which ranks first in the process of producing alcohol by cellulose fermentation, thus having economic feasibility, but also can change waste into valuable, thus solving the environment pollution problem of the papermaking industry.

Description

technical field

[0001] The invention relates to a method for producing energy alcohol by synchronous saccharification and fermentation of lignocellulosic raw materials, in particular to a method for producing alcohol by synchronous saccharification and fermentation of cellulase using the waste paper pulp as a raw material, belonging to microorganisms The field of fermentation production. Background technique

[0002] As the global oil price continues to rise, the production of energy alcohol is also increasing. Lignocellulosic feedstock is considered the only renewable feedstock for sustainable production of energy ethanol. However, almost all energy alcohol currently produced is produced from starch or sugar crops. Therefore, there is inevitably a situation of competition with food raw materials and an inability to meet the continuously growing demand for energy alcohol. The latest research shows that the greenhouse gas produced by starch alcohol production technology re...

Examples

Embodiment 1

[0020] The method for producing energy alcohol by fermenting waste paper pulp, the specific process is as follows:

[0021] (1) Seed preparation: The alcoholic yeast Saccharomyces Cerevisiae stored at -80°C was amplified step by step on a test tube slope and a shaker flask, and the number of cells obtained reached 10 8 cells / ml of yeast mash. Wherein the medium adopts YPD medium, that is, yeast powder 10g / L, peptone 10g / L, glucose 20g / L; 1.5% agar is added to the slope; the initial pH of the medium is 5.5;

[0022] (2) Preparation of fermentation medium and sterilization of raw materials: the amount of liquid in a 100ml serum bottle is 50ml, and the amount of waste paper pulp in the initial medium is 10g / L of cellulose, 1g / L of corn steep liquor; 0.1g / L of magnesium sulfate; natural pH. Seal the bottle with a rubber stopper and an aluminum cap, and sterilize at 115°C for 60 minutes;

[0023] (3) Synchronous saccharification and fermentation: add glucanase (Accellerase) acco...

Embodiment 2

[0026] The method for producing energy alcohol by fermenting waste paper pulp, the specific process is as follows:

[0027] (1) Seed preparation: The alcoholic yeast Saccharomyces Cerevisiae stored at -80°C was amplified step by step on a test tube slope and a shaker flask, and the number of cells obtained reached 10 8 cells / ml of yeast mash. Wherein the medium adopts YPD medium, that is, yeast powder 10g / L, peptone 10g / L, glucose 20g / L; 1.5% agar is added to the slope; the initial pH of the medium is 5.5;

[0028] (2) Preparation of fermentation medium and sterilization of raw materials: the amount of liquid in a 100ml serum bottle is 50ml, and the amount of waste paper pulp in the initial medium is 60g / L of cellulose, 6g / L of corn steep liquor; 0.6g / L of magnesium sulfate; natural pH. Seal the bottle with a rubber stopper and an aluminum cap, and sterilize at 130°C for 30 minutes;

[0029] (3) Simultaneous saccharification and fermentation: add glucanase (GC 220, Genencor...

Embodiment 3

[0032] The method for producing energy alcohol by fermenting waste paper pulp, the specific process is as follows:

[0033] (1) Seed preparation: The alcoholic yeast Saccharomyces Cerevisiae stored at -80°C was amplified step by step on a test tube slope and a shaker flask, and the number of cells obtained reached 10 8 individual / ml of yeast mash. Wherein the medium adopts YPD medium, that is, yeast powder 10g / L, peptone 10g / L, glucose 20g / L; 1.5% agar is added to the slope; the initial pH of the medium is 7.0;

[0034] (2) Preparation of fermentation medium and sterilization of raw materials: the amount of liquid in a 100ml serum bottle is 50ml, and the amount of waste paper pulp added in the initial medium is 40g / L of cellulose, 4g / L of corn steep liquor; 0.4g / L of magnesium sulfate; natural pH. Seal the bottle with a rubber stopper and an aluminum cap, and sterilize at 121°C for 45 minutes;

[0035] (3) Simultaneous saccharification and fermentation: Add glucanase (Spezy...

technical field

[0001] The invention relates to a method for producing energy alcohol by synchronous saccharification and fermentation of lignocellulosic raw materials, in particular to a method for producing alcohol by synchronous saccharification and fermentation of cellulase using the waste paper pulp as a raw material, belonging to microorganisms The field of fermentation production. Background technique

[0002] As the global oil price continues to rise, the production of energy alcohol is also increasing. Lignocellulosic feedstock is considered the only renewable feedstock for sustainable production of energy ethanol. However, almost all energy alcohol currently produced is produced from starch or sugar crops. Therefore, there is inevitably a situation of competition with food raw materials and an inability to meet the continuously growing demand for energy alcohol. The latest research shows that the greenhouse gas produced by starch alcohol production technology re...

Examples

Embodiment 1

[0020] The method for producing energy alcohol by fermenting waste paper pulp, the specific process is as follows:

[0021] (1) Seed preparation: The alcoholic yeast Saccharomyces Cerevisiae stored at -80°C was amplified step by step on a test tube slope and a shaker flask, and the number of cells obtained reached 10 8 cells / ml of yeast mash. Wherein the medium adopts YPD medium, that is, yeast powder 10g / L, peptone 10g / L, glucose 20g / L; 1.5% agar is added to the slope; the initial pH of the medium is 5.5;

[0022] (2) Preparation of fermentation medium and sterilization of raw materials: the amount of liquid in a 100ml serum bottle is 50ml, and the amount of waste paper pulp in the initial medium is 10g / L of cellulose, 1g / L of corn steep liquor; 0.1g / L of magnesium sulfate; natural pH. Seal the bottle with a rubber stopper and an aluminum cap, and sterilize at 115°C for 60 minutes;

[0023] (3) Synchronous saccharification and fermentation: add glucanase (Accellerase) acco...

Embodiment 2

[0026] The method for producing energy alcohol by fermenting waste paper pulp, the specific process is as follows:

[0027] (1) Seed preparation: The alcoholic yeast Saccharomyces Cerevisiae stored at -80°C was amplified step by step on a test tube slope and a shaker flask, and the number of cells obtained reached 10 8 cells / ml of yeast mash. Wherein the medium adopts YPD medium, that is, yeast powder 10g / L, peptone 10g / L, glucose 20g / L; 1.5% agar is added to the slope; the initial pH of the medium is 5.5;

[0028] (2) Preparation of fermentation medium and sterilization of raw materials: the amount of liquid in a 100ml serum bottle is 50ml, and the amount of waste paper pulp in the initial medium is 60g / L of cellulose, 6g / L of corn steep liquor; 0.6g / L of magnesium sulfate; natural pH. Seal the bottle with a rubber stopper and an aluminum cap, and sterilize at 130°C for 30 minutes;

[0029] (3) Simultaneous saccharification and fermentation: add glucanase (GC 220, Genencor...

Embodiment 3

[0032] The method for producing energy alcohol by fermenting waste paper pulp, the specific process is as follows:

[0033] (1) Seed preparation: The alcoholic yeast Saccharomyces Cerevisiae stored at -80°C was amplified step by step on a test tube slope and a shaker flask, and the number of cells obtained reached 10 8 individual / ml of yeast mash. Wherein the medium adopts YPD medium, that is, yeast powder 10g / L, peptone 10g / L, glucose 20g / L; 1.5% agar is added to the slope; the initial pH of the medium is 7.0;

[0034] (2) Preparation of fermentation medium and sterilization of raw materials: the amount of liquid in a 100ml serum bottle is 50ml, and the amount of waste paper pulp added in the initial medium is 40g / L of cellulose, 4g / L of corn steep liquor; 0.4g / L of magnesium sulfate; natural pH. Seal the bottle with a rubber stopper and an aluminum cap, and sterilize at 121°C for 45 minutes;

[0035] (3) Simultaneous saccharification and fermentation: Add glucanase (Spezy...