Sulphation modification method of codonopsis pilosula polysaccharide

A Codonopsis polysaccharide, sulfation technology, applied in the field of sulfation modification of Codonopsis polysaccharide, can solve the problem of low biological activity, achieve strong antiviral effect and improve antiviral activity

CN101781372BInactive Publication Date: 2012-04-25NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2012-04-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a sulphation modification method of codonopsis pilosula polysaccharide, belonging to the traditional Chinese medicine polysaccharide structure modification technical field. Codonopsis pilosula polysaccharide is extracted and purified, chlorosulfonic acid-pyridine method is used for carrying out sulphation modification, throughput and substitution ratio are taken as indexes, L9(34) orthogonal experiment method is used for optimization on three factors of reagent matching ratio, reaction temperature and reaction time, and the optimal modification conditions that mass ratio of chlorosulfonic acid and pyridine is 1:6, reaction temperature is 80 DEG C and reaction time is three hours are determined, and the obtained sulphated codonopsis pilosula polysaccharide is high in throughput and substitution ratio and strong in activity. The invention can obviously improve antiviral activity of codonopsis pilosula polysaccharide.
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Description

1. Technical field

[0001] The invention discloses a sulfation modification method of Codonopsis polysaccharides, which belongs to the technical field of structure modification of traditional Chinese medicine polysaccharides. 2. Background technology

[0002] Polysaccharide is another important biological macromolecule substance besides protein and nucleic acid in organisms. It has many functions such as resisting infection, enhancing immunity, antioxidation, preventing and treating tumor, viral hepatitis, rheumatoid disease, AIDS and other immune damage or immunodeficiency diseases. aspects of function and biological activity. With the rapid development of chemistry and biology and the improvement of separation technology, the biological functions of polysaccharides, especially the participation in all time and space functions of life as living substances, have broken through the traditional concept of polysaccharides as supporting tissues and energy sources. With a wider r...

Examples

Embodiment Construction

[0014] 1. Extraction of Codonopsis polysaccharide

[0015] Decoction with water - ultrasonic alcohol precipitation. Take 500g of dangshen decoction pieces, crush them, put them in a Soxhlet extractor, and reflux them with 80% ethanol for 3 times until the reflux liquid is almost colorless, add the dregs to 20 times of water, and pretreat with 80°C ultrasonic (power 80W, 10min each time) 2 times, and then decocted 3 times, the first time 2h, the second time 1h, the third time 0.5h, combined the three filtrates, concentrated to 650mL. Add 95% ethanol to make the alcohol concentration reach 80%, let stand overnight, centrifuge to get the precipitate, wash with ethanol and acetone, and dry to obtain crude Codonopsis polysaccharide.

[0016] 2. Purification of Codonopsis polysaccharide

[0017] (1) Deproteinization: the crude Codonopsis polysaccharide was formulated into a 5% aqueous solution, and part of the protein was removed by repeated freezing and thawing (freezing at -20° ...