Sulfated mesona chinensis benth polysaccharide and preparation method thereof

A technology of jelly grass and sulfation, which is applied in the directions of antidote and drug combination, can solve the problems of low biological activity of polysaccharides, and achieve the effect of improving the protective effect.

CN110003351AActive Publication Date: 2019-07-12NANCHANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2019-07-12

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Abstract

The invention relates to the technical field of structural modification of polysaccharide, particularly relates to sulfated mesona chinensis benth polysaccharide and a preparation method thereof, andbelongs to the technical field of structural modification of polysaccharide. Mesona chinensis benth polysaccharide is extracted and purified, and is subjected to sulfated modification by a chlorosulfonic acid-pyridine method. The protective effect of a product on RAW264.7 cells is taken as an index, three factors including reagent ratio, reaction temperature and reaction time are optimized by orthogonal experiments, the optimum modification conditions are determined as follows: the mass ratio of chlorosulfonic acid to pyridine is 1: 2, the reaction temperature is 60 DEG C, the reaction time is90 min, and the sulfated mesona chinensis benth polysaccharide has the strongest protective effect on the RAW264.7 cells. The protective effect can be achieved by increasing the level of SOD and reducing the content of MDA. The protective effect of the mesona chinensis benth polysaccharide on oxidative stress of the RAW264.7 cells can be improved remarkably.
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Description

technical field

[0001] The invention belongs to the technical field of structural modification of polysaccharides, and in particular relates to a sulfated jelly jelly polysaccharide and a preparation method thereof. Background technique

[0002] Polysaccharides are widely found in animals, plants and microbial tissues. They are natural polymer compounds formed by the polymerization of monosaccharides. They are another important biological macromolecule in addition to proteins and nucleic acids in organisms. They have anti-virus, anti-tumor, anti- Oxidation, immune regulation and other biological activities. The biological activity of polysaccharides is closely related to its structure, especially when some chemical groups are introduced, the flexibility and spatial structure of sugar chains will change, thereby causing changes in the biological activities of polysaccharides or new activities of polysaccharides. Therefore, the appropriate chemical modification of the polysac...

Examples

Embodiment Construction

[0020] 1. Extraction of jelly jelly polysaccharide

[0021] Soak jelly grass with 9 times the volume of 80% ethanol for 20 hours after crushing, and air-dry the residue in a cool place. After pretreatment, add 15 times the volume of the jelly jelly, extract with boiling water for 2 hours, filter the supernatant with 120-mesh gauze, repeat the extraction twice from the filter residue, combine the filtrates, concentrate and centrifuge to obtain the jelly jelly crude polysaccharide solution.

[0022] 2. Purification of jelly jelly polysaccharide

[0023] Use 80% ethanol to precipitate the crude polysaccharide solution at 4°C for 20 hours, repeat it 4 times, and put it on the D301 macroporous resin chromatography column, elute the resin column with 0.2M NaCl solution, control the flow rate at 1.8mL / min, collect eluent. Concentration, dialysis to remove small molecular substances, and freeze-drying to obtain neutral and uniform jelly polysaccharide (MP).

[0024] 3. Sulfation mo...