Natural anti- virus and composition comprising thereof

Abstract

Disclosed herein are a natural antiviral agent having high inhibitory activity against a broad spectrum of viruses without adverse side effects, obtainable using lactic acid bacteria isolated from kimchi, and a composition comprising the natural antiviral agent. The natural antiviral agent is safe to the body and can be applied to compositions, such as fodder, beverages, food, medicine, and cosmetics, imparting antimicrobial activity thereto.

Description

[0001] This application is the international application number PCT / KR2005 / 002348, the international application date is July 20, 2005, the Chinese application number is 200580024612.3, and the date of entering China is January 22, 2007. object" divisional application. technical field

[0002] The present invention relates to a natural antiviral agent and compositions comprising the same. More specifically, the present invention relates to a natural antiviral agent prepared by using lactic acid bacteria isolated from Kimchi (Kimchi), which exhibits high inhibitory activity against virus growth without side effects and is safe to the body. It relates to compositions, such as beverages, feeds, foods, medicines, cosmetics, etc., in which said natural antiviral agents are included. Background technique

[0003] The influenza virus that causes the annual influenza epidemic is a basic single-stranded RNA virus that has an envelope with a size of 1 / 10,000mm enclosing the RNA. Acc...

Examples

Embodiment Construction

[0034] Preparation of Kimchi Lactic Acid Bacteria Culture Solution

[0035] Among the kimchi lactic acid bacteria, Leuconostoc kimchi (KCTC2386), Leuconostoc enterica subsp. enterica (KCTC3530), Leuconostoc citreum (KCTC3524), Leuconostoc lactis (KCTC3528), Lactobacillus plantarum (KCCM11322), and Weissella koreensis (KCTC3746) was used to prepare the culture medium.

[0036] First, 100 ml of MRS (Difco) broth was autoclaved at 121° C. for 15 minutes, and Kimchi lactic acid bacteria were inoculated into the broth, and shake cultured at 30° C. for 16 hours.

[0037] After the obtained culture was centrifuged at 5000×g for 3 min, the supernatant was filtered through a membrane filter having a pore size of 0.22 to obtain a sterile solution.

[0038] Subsequently, this sterile solution was passed through a membrane with a cut-off of 10,000 Da. The resulting filtrate was concentrated using a membrane with a 1,000 Da cut-off. The concentrate thus obtained was filtered again throu...

[0001] This application is the international application number PCT / KR2005 / 002348, the international application date is July 20, 2005, the Chinese application number is 200580024612.3, and the date of entering China is January 22, 2007. object" divisional application. technical field

[0002] The present invention relates to a natural antiviral agent and compositions comprising the same. More specifically, the present invention relates to a natural antiviral agent prepared by using lactic acid bacteria isolated from Kimchi (Kimchi), which exhibits high inhibitory activity against virus growth without side effects and is safe to the body. It relates to compositions, such as beverages, feeds, foods, medicines, cosmetics, etc., in which said natural antiviral agents are included. Background technique

[0003] The influenza virus that causes the annual influenza epidemic is a basic single-stranded RNA virus that has an envelope with a size of 1 / 10,000mm enclosing the RNA. Acc...

Examples

Embodiment Construction

[0034] Preparation of Kimchi Lactic Acid Bacteria Culture Solution

[0035] Among the kimchi lactic acid bacteria, Leuconostoc kimchi (KCTC2386), Leuconostoc enterica subsp. enterica (KCTC3530), Leuconostoc citreum (KCTC3524), Leuconostoc lactis (KCTC3528), Lactobacillus plantarum (KCCM11322), and Weissella koreensis (KCTC3746) was used to prepare the culture medium.

[0036] First, 100 ml of MRS (Difco) broth was autoclaved at 121° C. for 15 minutes, and Kimchi lactic acid bacteria were inoculated into the broth, and shake cultured at 30° C. for 16 hours.

[0037] After the obtained culture was centrifuged at 5000×g for 3 min, the supernatant was filtered through a membrane filter having a pore size of 0.22 to obtain a sterile solution.

[0038] Subsequently, this sterile solution was passed through a membrane with a cut-off of 10,000 Da. The resulting filtrate was concentrated using a membrane with a 1,000 Da cut-off. The concentrate thus obtained was filtered again throu...

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