Purifying process of pedicularioside A

Abstract

The invention discloses a purifying process of pedicularioside A. The process is realized in the method comprising the steps that pedicularis spicata pall is used as raw materials, an ethanol solution is added, and microwave extraction is performed; ethyl alcohol is recycled from extract, extractum is dispersed by adding hot water, polar macroporous resin is added for adsorption, and elution is performed; eluant is ultra-filtrated through an ultra-filtration membrane, concentration is performed through a nanofiltration membrane, drying is performed, and crude products are obtained; the crude products are dissolved through the ethyl alcohol in a backflow mode, and placed, sedimentation is performed, filtration is performed, and the pedicularioside A is obtained. The process has the advantages that the pedicularioside A made in the method is high in purity, the method is simple, convenient to achieve, and practical, cost is low, and industrialization is achieved easily.

Description

technical field

[0001] The invention relates to a purification process of equine artemisinin A, which belongs to the field of medicine. Background technique

[0002] Pedicularioside A is a phenylpropanoid glycoside compound with the molecular formula C 34 h 44 o 19 , molecular weight 756.71, easily soluble in hot water and methanol, insoluble in cold water, the structural formula is:

[0003]

[0004] Artemisinin A is derived from the whole plant of Artemisia spicata Pall. in the family Scrophulariaceae. Modern pharmacological studies have shown that equine artemisinin A has a variety of activities. Equine artemisinin A has anti-oxidation effect and can inhibit the oxidative hemolysis of red blood cells. Its effect is equivalent to that of the antioxidant propyl gallate. The drug inhibition rate was 56.8%; it has anti-tumor effect, and its IC50 (μg / mL) is 94.8±2.0 (liver cancer SMMC-7721), 97.6±15.0 (lung adenocarcinoma L342), 101.6±2.8 (gastric adenocarcinoma MGc-80...

Examples

Embodiment 1

[0025] Grind Artemisia fringa to 20 mesh, take 5kg and put it into a microwave extraction tank, add 50L of 45% ethanol solution for microwave extraction for 10min, the microwave extraction power is 10kW, extract 3 times, combine the extracts, recover the ethanol under reduced pressure until it is in the form of an extract, add water to dissolve, filter The insoluble matter was removed, and the filtrate was added to a 5L D101 macroporous resin column for adsorption, washed with water until the sugar reaction was negative, and then 20L of 60% ethanol solution was used to elute the active ingredient, and the eluate was collected, and the eluate was added to a hollow composite membrane supernatant with a molecular weight cut-off of 3000. Membrane ultrafiltration, the permeate is added to the hollow composite nanofiltration membrane with a molecular weight cut-off of 100 to concentrate, the inlet pressure is controlled to 0.8Mpa to obtain a concentrated solution, the concentrated sol...

Embodiment 2

[0028] Grind Artemisia fringa to 60 mesh, take 5kg and put it into a microwave extraction tank, add 50L of 45% ethanol solution for microwave extraction for 20min, the microwave extraction power is 1kW, extract twice, combine the extracts, recover the ethanol under reduced pressure until it is in the form of an extract, add water to dissolve, filter The insoluble matter was removed, and the filtrate was added to a 5L AB-8 macroporous resin column for adsorption, washed with water until the sugar reaction was negative, and then 35L of 50% ethanol solution was used to elute the active ingredient, the eluate was collected, and the eluate was added to a hollow compound with a molecular weight cut-off of 10,000. Membrane ultrafiltration Membrane ultrafiltration, the permeate is added to the hollow composite nanofiltration membrane with a molecular weight cut-off of 100 to concentrate, the inlet pressure is controlled to 0.5Mpa to obtain a concentrated solution, and the concentrated s...

Embodiment 3

[0031] Grind Artemisia fringa to 80 mesh, take 5 kg and put it into a microwave extraction tank, add 50L of 45% ethanol solution for microwave extraction for 20 minutes, microwave extraction power is 5kW, extract 3 times, combine the extracts and recover ethanol under reduced pressure until it becomes an extract, add water to dissolve, filter out For insoluble matter, add the filtrate to 5L SPD100 macroporous resin column for adsorption, wash it with water until the sugar reaction is negative, then use 28L 70% ethanol solution to elute the active ingredient, collect the eluate, add the eluate to ultrafiltration with a hollow composite membrane with a molecular weight cut-off of 6000 Membrane ultrafiltration, the permeate is added to the hollow composite nanofiltration membrane with a molecular weight cut-off of 100 to concentrate, and the inlet pressure is controlled to 0.6Mpa to obtain a concentrated solution. The concentrated solution is dried to obtain a crude product, which ...

technical field

[0001] The invention relates to a purification process of equine artemisinin A, which belongs to the field of medicine. Background technique

[0002] Pedicularioside A is a phenylpropanoid glycoside compound with the molecular formula C 34 h 44 o 19 , molecular weight 756.71, easily soluble in hot water and methanol, insoluble in cold water, the structural formula is:

[0003]

[0004] Artemisinin A is derived from the whole plant of Artemisia spicata Pall. in the family Scrophulariaceae. Modern pharmacological studies have shown that equine artemisinin A has a variety of activities. Equine artemisinin A has anti-oxidation effect and can inhibit the oxidative hemolysis of red blood cells. Its effect is equivalent to that of the antioxidant propyl gallate. The drug inhibition rate was 56.8%; it has anti-tumor effect, and its IC50 (μg / mL) is 94.8±2.0 (liver cancer SMMC-7721), 97.6±15.0 (lung adenocarcinoma L342), 101.6±2.8 (gastric adenocarcinoma MGc-80...

Examples

Embodiment 1

[0025] Grind Artemisia fringa to 20 mesh, take 5kg and put it into a microwave extraction tank, add 50L of 45% ethanol solution for microwave extraction for 10min, the microwave extraction power is 10kW, extract 3 times, combine the extracts, recover the ethanol under reduced pressure until it is in the form of an extract, add water to dissolve, filter The insoluble matter was removed, and the filtrate was added to a 5L D101 macroporous resin column for adsorption, washed with water until the sugar reaction was negative, and then 20L of 60% ethanol solution was used to elute the active ingredient, and the eluate was collected, and the eluate was added to a hollow composite membrane supernatant with a molecular weight cut-off of 3000. Membrane ultrafiltration, the permeate is added to the hollow composite nanofiltration membrane with a molecular weight cut-off of 100 to concentrate, the inlet pressure is controlled to 0.8Mpa to obtain a concentrated solution, the concentrated sol...

Embodiment 2

[0028] Grind Artemisia fringa to 60 mesh, take 5kg and put it into a microwave extraction tank, add 50L of 45% ethanol solution for microwave extraction for 20min, the microwave extraction power is 1kW, extract twice, combine the extracts, recover the ethanol under reduced pressure until it is in the form of an extract, add water to dissolve, filter The insoluble matter was removed, and the filtrate was added to a 5L AB-8 macroporous resin column for adsorption, washed with water until the sugar reaction was negative, and then 35L of 50% ethanol solution was used to elute the active ingredient, the eluate was collected, and the eluate was added to a hollow compound with a molecular weight cut-off of 10,000. Membrane ultrafiltration Membrane ultrafiltration, the permeate is added to the hollow composite nanofiltration membrane with a molecular weight cut-off of 100 to concentrate, the inlet pressure is controlled to 0.5Mpa to obtain a concentrated solution, and the concentrated s...

Embodiment 3

[0031] Grind Artemisia fringa to 80 mesh, take 5 kg and put it into a microwave extraction tank, add 50L of 45% ethanol solution for microwave extraction for 20 minutes, microwave extraction power is 5kW, extract 3 times, combine the extracts and recover ethanol under reduced pressure until it becomes an extract, add water to dissolve, filter out For insoluble matter, add the filtrate to 5L SPD100 macroporous resin column for adsorption, wash it with water until the sugar reaction is negative, then use 28L 70% ethanol solution to elute the active ingredient, collect the eluate, add the eluate to ultrafiltration with a hollow composite membrane with a molecular weight cut-off of 6000 Membrane ultrafiltration, the permeate is added to the hollow composite nanofiltration membrane with a molecular weight cut-off of 100 to concentrate, and the inlet pressure is controlled to 0.6Mpa to obtain a concentrated solution. The concentrated solution is dried to obtain a crude product, which ...

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