Method for extracting aucubin from folium cortex eucommiae

Abstract

The invention discloses a method for extracting aucubin from folium cortex eucommiae, aucubin powder is prepared by crushing of the folium cortex eucommiae, cellulase assisted extraction in medium temperature water, conventional filtration, impurity removal by a ultrafiltration membrane, nanofiltration concentration, separation and purification by macroporous resin, concentration and drying; according to the method, the aucubin is extracted from the folium cortex eucommiae by cellulase assisted extraction in the medium temperature water, the extraction rate can reach 95%, the water extraction temperature is lower, and the extraction time shorter; the ultrafiltration membrane is used for impurity removal of an extracting solution, and separation of other by-products, the membrane operation process is simple, the membrane flux can be stable for a long time, the membrane is easy to clean and long in service life and realizes industrialization; an nanofiltration membrane is used for concentration of the impurity removed extracting solution, the load of follow-up processes can be reduced, and the service life of resin is lengthened; the COD value of a nanofiltration filtrate is less than or equal to 100, the nanofiltration filtrate can be reused in the production process, and basically no waste water is not discharged; the macroporous adsorption resin can effectively purify the aucubin, purity of the aucubin in the prepared powder is greater than 62%, and the extraction rate is more than 2.8%.

Description

technical field

[0001] The invention relates to a method for extracting and separating effective plant components in the pharmaceutical industry, in particular to a process for extracting aucubin from leaves of Eucommia ulmoides. . Background technique

[0002] The chemical name of aucubin (aucubin, AU) is β-D-glucopyranoside, which is an iridoid glycoside compound, the molecular formula is C15H2209, the molecular weight is 346.331, the CAS number is 479-98-1, and the melting point is 181°C , Soluble in water, ethanol and methanol, almost insoluble in chloroform, ether and petroleum ether. This compound is mainly found in Eucommia ulmoides ( Lucommia ulmoides Oliver ), the dogwood plant Peachleaf coral ( Aucubachinensis Benth ), plantain plant car ( Plantago asiatica L ) and other plants, it is one of the active ingredients of Chinese herbal medicines such as eucommia, plantain, and rehmannia glutinosa. It has many pharmacological activities such as liver protection an...

Examples

Embodiment Construction

[0026] Example 1, see the attached figure 1 10Kg of dried eucommia leaves was taken, crushed and passed through a 100-mesh sieve, added 120Kg of water, adjusted to a temperature of 55°C, kept at a constant temperature and adjusted to a pH value of 4.5, then added 20g of cellulase, extracted for 60min under this condition, filtered, The filter residue was extracted for the second time according to the above extraction conditions, the two filtrates were combined, and filtered conventionally, the extraction rate of aucubin was 96.0%; the operating conditions were P=0.3Mpa, T=45°C, pH=4.5, Q=70L 8L / m 2 h; at operating conditions P=1.5Mpa, T=50°C, pH=4.5, Q=20L / min, the ultrafiltration filtrate is passed through a 200 molecular weight nanofiltration membrane again, and the ultrafiltration filtrate is concentrated by 16. 8 times, the removal rate of inorganic salt ions is 63%, and the average flux of the nanofiltration membrane is 18.4 L / m 2 h, the COD of the nanofiltration filtr...

technical field

[0001] The invention relates to a method for extracting and separating effective plant components in the pharmaceutical industry, in particular to a process for extracting aucubin from leaves of Eucommia ulmoides. . Background technique

[0002] The chemical name of aucubin (aucubin, AU) is β-D-glucopyranoside, which is an iridoid glycoside compound, the molecular formula is C15H2209, the molecular weight is 346.331, the CAS number is 479-98-1, and the melting point is 181°C , Soluble in water, ethanol and methanol, almost insoluble in chloroform, ether and petroleum ether. This compound is mainly found in Eucommia ulmoides ( Lucommia ulmoides Oliver ), the dogwood plant Peachleaf coral ( Aucubachinensis Benth ), plantain plant car ( Plantago asiatica L ) and other plants, it is one of the active ingredients of Chinese herbal medicines such as eucommia, plantain, and rehmannia glutinosa. It has many pharmacological activities such as liver protection an...

Examples

Embodiment Construction

[0026] Example 1, see the attached figure 1 10Kg of dried eucommia leaves was taken, crushed and passed through a 100-mesh sieve, added 120Kg of water, adjusted to a temperature of 55°C, kept at a constant temperature and adjusted to a pH value of 4.5, then added 20g of cellulase, extracted for 60min under this condition, filtered, The filter residue was extracted for the second time according to the above extraction conditions, the two filtrates were combined, and filtered conventionally, the extraction rate of aucubin was 96.0%; the operating conditions were P=0.3Mpa, T=45°C, pH=4.5, Q=70L 8L / m 2 h; at operating conditions P=1.5Mpa, T=50°C, pH=4.5, Q=20L / min, the ultrafiltration filtrate is passed through a 200 molecular weight nanofiltration membrane again, and the ultrafiltration filtrate is concentrated by 16. 8 times, the removal rate of inorganic salt ions is 63%, and the average flux of the nanofiltration membrane is 18.4 L / m 2 h, the COD of the nanofiltration filtr...

Images