Magnetic resonance imaging guided targeting metal organic framework drug carrier preparation method

Abstract

The invention relates to a magnetic resonance imaging guided targeting metal organic framework drug carrier preparation method, which comprises: adopting a hydrothermal method to prepare magnetic spheres with carboxyl on the surface, completely and uniformly mixing the magnetic spheres and a metal organic framework synthesis precursor solution, adopting a one-pot method to prepare a magnetic resonance imaging guided metal organic framework drug carrier under a hydrothermal condition, and finally modifying targeting molecules on the metal organic framework so as to obtain the magnetic resonance imaging guided targeting metal organic framework drug carrier. According to the present invention, the drug carrier prepared through the method integrates characteristics of the targeting molecules, the magnetic spheres and the metal organic framework, such that the triple functions such as targeting property, magnetic resonance imaging and drug loading are provided, the magnetic resonance imaging-assisted visualized targeting drug delivery can be achieved, and the difference in the time and in the space during the diagnosis and therapy process is avoided; and the preparation method is simple and easy performing, the scale production is easily achieved, and great development potential and application values are provided in the field of biomedicine and pharmaceutical engineering.

Description

technical field

[0001] The invention relates to the technical field of preparation of nanomaterials and application of drug delivery, in particular to a method for preparing a magnetic resonance imaging-guided targeting metal-organic framework drug carrier. Background technique

[0002] A drug carrier is a submicron drug delivery system with a particle size of 10-1000nm. It is generally made of natural polymer or synthetic polymer material as a matrix, and the drug is encapsulated in the subparticle or adsorbed on the surface of the subparticle, and transported to the lesion site. Play a role. Drug carriers can not only solve the problems of low solubility, poor stability, non-targeting, large side effects, and easy to be rapidly metabolized and cleared by existing small molecule drugs, but also control the release rate of drugs and increase the permeability of biomembranes. Sex, change the distribution of drugs in the body, and improve the bioavailability of drugs. At pre...

Examples

Embodiment 1

[0024] A method for preparing a magnetic resonance imaging-guided targeted metal-organic framework drug carrier, comprising the following steps:

[0025] 1) Preparation of magnetic balls: Add 0.54 g of ferric chloride hexahydrate and 21.6 g of ethylene glycol into a 50 mL round-bottomed flask, and dissolve with ultrasonic stirring. Under magnetic stirring, add 0.2g of polyacrylic acid, 1.5g of water, and 1.2g of urea to the above solution in sequence. After the mixture is stirred for 15 to 30 minutes, transfer it to a 30mL hydrothermal reaction kettle, seal it, and react it in a blast drying oven at 200°C. 12 hours. After the reaction, the reaction kettle is naturally cooled to room temperature, the reactant is magnetically sucked by ethanol for three to five times, and then vacuum-dried for 12 to 24 hours to obtain black magnetic balls.

[0026] 2) Preparation of magnetic sphere dispersion: Take 25 mg of magnetic spheres in a 25 mL vial, add 10 mL of N,N-dimethylformamide, a...

Embodiment 2

[0031]A method for preparing a magnetic resonance imaging-guided targeted metal-organic framework drug carrier, comprising the following steps:

[0032] 1) Preparation of magnetic spheres: Add 0.81 g of ferric chloride hexahydrate and 32.4 g of ethylene glycol into a 100 mL round-bottomed flask, and dissolve with ultrasonic stirring. Under magnetic stirring, add 0.3g of polyacrylic acid, 2.25g of water, and 1.8g of urea to the above solution in sequence. After the mixture is stirred for 15 to 30 minutes, transfer it to a 50mL hydrothermal reactor, seal it, and react in a blast drying oven at 220°C. 8 hours. After the reaction, the reaction kettle is naturally cooled to room temperature, the reactant is magnetically sucked by ethanol for three to five times, and then vacuum-dried for 12 to 24 hours to obtain black magnetic balls.

[0033] 2) Preparation of magnetic sphere dispersion: Take 50 mg of magnetic spheres in a 25 mL vial, add 5 mL of methanol, and sonicate for 30 minu...

Embodiment 3

[0038] A method for preparing a magnetic resonance imaging-guided targeted metal-organic framework drug carrier, comprising the following steps:

[0039] 1) Preparation of magnetic spheres: Add 1.62 g of ferric chloride hexahydrate and 64.8 g of ethylene glycol into a 250 mL round bottom flask, and dissolve them with ultrasonic stirring. Under magnetic stirring, add 0.6g of polyacrylic acid, 4.5g of water, and 3.6g of urea to the above solution in sequence. After the mixture is stirred for 15 to 30 minutes, transfer it to a 100mL hydrothermal reactor, seal it, and react in a blast drying oven at 210°C. 10 hours. After the reaction, the reaction kettle is naturally cooled to room temperature, the reactant is magnetically sucked by ethanol for three to five times, and then vacuum-dried for 12 to 24 hours to obtain black magnetic balls.

[0040] 2) Preparation of magnetic sphere dispersion: Take 75 mg of magnetic spheres in a 25 mL vial, add 10 mL of N,N-dimethylformamide, and s...

technical field

[0001] The invention relates to the technical field of preparation of nanomaterials and application of drug delivery, in particular to a method for preparing a magnetic resonance imaging-guided targeting metal-organic framework drug carrier. Background technique

[0002] A drug carrier is a submicron drug delivery system with a particle size of 10-1000nm. It is generally made of natural polymer or synthetic polymer material as a matrix, and the drug is encapsulated in the subparticle or adsorbed on the surface of the subparticle, and transported to the lesion site. Play a role. Drug carriers can not only solve the problems of low solubility, poor stability, non-targeting, large side effects, and easy to be rapidly metabolized and cleared by existing small molecule drugs, but also control the release rate of drugs and increase the permeability of biomembranes. Sex, change the distribution of drugs in the body, and improve the bioavailability of drugs. At pre...

Examples

Embodiment 1

[0024] A method for preparing a magnetic resonance imaging-guided targeted metal-organic framework drug carrier, comprising the following steps:

[0025] 1) Preparation of magnetic balls: Add 0.54 g of ferric chloride hexahydrate and 21.6 g of ethylene glycol into a 50 mL round-bottomed flask, and dissolve with ultrasonic stirring. Under magnetic stirring, add 0.2g of polyacrylic acid, 1.5g of water, and 1.2g of urea to the above solution in sequence. After the mixture is stirred for 15 to 30 minutes, transfer it to a 30mL hydrothermal reaction kettle, seal it, and react it in a blast drying oven at 200°C. 12 hours. After the reaction, the reaction kettle is naturally cooled to room temperature, the reactant is magnetically sucked by ethanol for three to five times, and then vacuum-dried for 12 to 24 hours to obtain black magnetic balls.

[0026] 2) Preparation of magnetic sphere dispersion: Take 25 mg of magnetic spheres in a 25 mL vial, add 10 mL of N,N-dimethylformamide, a...

Embodiment 2

[0031]A method for preparing a magnetic resonance imaging-guided targeted metal-organic framework drug carrier, comprising the following steps:

[0032] 1) Preparation of magnetic spheres: Add 0.81 g of ferric chloride hexahydrate and 32.4 g of ethylene glycol into a 100 mL round-bottomed flask, and dissolve with ultrasonic stirring. Under magnetic stirring, add 0.3g of polyacrylic acid, 2.25g of water, and 1.8g of urea to the above solution in sequence. After the mixture is stirred for 15 to 30 minutes, transfer it to a 50mL hydrothermal reactor, seal it, and react in a blast drying oven at 220°C. 8 hours. After the reaction, the reaction kettle is naturally cooled to room temperature, the reactant is magnetically sucked by ethanol for three to five times, and then vacuum-dried for 12 to 24 hours to obtain black magnetic balls.

[0033] 2) Preparation of magnetic sphere dispersion: Take 50 mg of magnetic spheres in a 25 mL vial, add 5 mL of methanol, and sonicate for 30 minu...

Embodiment 3

[0038] A method for preparing a magnetic resonance imaging-guided targeted metal-organic framework drug carrier, comprising the following steps:

[0039] 1) Preparation of magnetic spheres: Add 1.62 g of ferric chloride hexahydrate and 64.8 g of ethylene glycol into a 250 mL round bottom flask, and dissolve them with ultrasonic stirring. Under magnetic stirring, add 0.6g of polyacrylic acid, 4.5g of water, and 3.6g of urea to the above solution in sequence. After the mixture is stirred for 15 to 30 minutes, transfer it to a 100mL hydrothermal reactor, seal it, and react in a blast drying oven at 210°C. 10 hours. After the reaction, the reaction kettle is naturally cooled to room temperature, the reactant is magnetically sucked by ethanol for three to five times, and then vacuum-dried for 12 to 24 hours to obtain black magnetic balls.

[0040] 2) Preparation of magnetic sphere dispersion: Take 75 mg of magnetic spheres in a 25 mL vial, add 10 mL of N,N-dimethylformamide, and s...

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