Method for preparing antioxidative peptide liquid of collagen gel with combination of ultrasonic treatment and simulative digestion

Abstract

The invention discloses a method for preparing an antioxidative peptide liquid of collagen gel with combination of ultrasonic treatment and simulative digestion and belongs to the technical field of food processing technology and protein utilization. According to the adopted preparation process, the collagen gel is prepared from pepsin-soluble collagen taken as a raw material through dissolution, dialysis, ultrasonic treatment and self-assembly under a water bath, an in-vitro simulative gastrointestinal digestion technology is used, a bionic dynamic rat gastric digestion system capable of simulating peristole frequency and amplitude and simulating a secretory gland to secrete gastric digestive juice is used, then intestinal digestion is simulated, and two-step enzyme digestion is adopted by means of pepsin-trypsin. The enzyme degradation rate is increased greatly, and the rate of utilization of the raw material is increased. A dynamic model is used for simulating gastrointestinal digestion processes and is closer to a human body simulative digestion environment in an environment at 37 DEG C, the process is more efficient, and the method is safer and more reliable as compared with an artificial synthesis method and a fermentation method for antioxidative peptides.

Description

technical field

[0001] The invention belongs to the field of food processing technology and protein utilization technology, and specifically relates to the preparation of antioxidant polypeptide liquid by using ultrasonic pretreatment collagen gel combined with bionic dynamic rat stomach digestion system and simulated intestinal digestion. Background technique

[0002] Collagen gel is a collagen molecule that spontaneously organizes and aggregates in vitro through the force of non-covalent bonds without external force to form an ordered and stable supramolecular structure or molecular aggregate. Collagen aggregates from molecular structure to form filaments, and then aggregates to form collagen fibers and collagen fiber bundles. This process is completed in three stages: nucleation stage, growth stage and equilibrium stage. In recent years, the research of ultrasound in the fields of biology and chemistry is very popular. Ultrasound has good applications in various fields du...

Examples

example 1

[0034]Weigh 0.04g enzymatically soluble collagen (collagen is prepared by the laboratory, chicken feet skin is removed by a solution of 20% (w / v) NaCl (0.05mol / L Tris-HCl, pH7.5) at 4°C Impurities such as miscellaneous proteins, fats and pigments. Pretreated chicken paw skin is extracted with precooled 0.5M acetic acid containing 0.1% (w / v) pepsin (2500U / mg), centrifuged, and the supernatant salting out gained precipitation is vacuum Freeze-drying is enzyme-soluble collagen.), with 0.5M acetic acid, prepared into a 0.02g / mL collagen solution, and dialyzed against phosphate buffer (200mM, pH7.4) in a refrigerator at 4°C. After the dialysis, the solution was centrifuged at 20000×g for 15 min, and the supernatant was taken. In a water bath at 30°C, use a single-frequency ultrasonic device at 270W / 60kHz for 5 minutes, and heat in a water bath at 30°C for 55 minutes to form collagen molecules and self-assemble to form a collagen gel. The collagen gel sponge was obtained by vacuum ...

example 2

[0037] Weigh 0.04g of enzyme-soluble collagen (made in the laboratory, same as Example 1), prepare 0.02g / mL collagen solution with 0.5M acetic acid, and dialyze against phosphate buffered saline (200mM, pH7.4) at 4°C . After the dialysis, the solution was centrifuged at 20000×g for 15 min, and the supernatant was taken. In a water bath at 30°C, use a single-frequency ultrasonic device at 270W / 60kHz for 5 minutes, and heat in a water bath at 30°C for 55 minutes to form collagen molecules and self-assemble to form a collagen gel. The collagen gel sponge was obtained by vacuum freeze-drying, which was used in the next step.

[0038] In the test, 2 mL of 0.02 g / mL collagen gel solution was prepared with distilled water to simulate gastrointestinal digestion in vitro. The bionic dynamic mouse stomach digestion system is set with an external electric compression device, which is adjusted by the motor to be close to the real mouse stomach peristaltic contraction frequency, that is,...

technical field

[0001] The invention belongs to the field of food processing technology and protein utilization technology, and specifically relates to the preparation of antioxidant polypeptide liquid by using ultrasonic pretreatment collagen gel combined with bionic dynamic rat stomach digestion system and simulated intestinal digestion. Background technique

[0002] Collagen gel is a collagen molecule that spontaneously organizes and aggregates in vitro through the force of non-covalent bonds without external force to form an ordered and stable supramolecular structure or molecular aggregate. Collagen aggregates from molecular structure to form filaments, and then aggregates to form collagen fibers and collagen fiber bundles. This process is completed in three stages: nucleation stage, growth stage and equilibrium stage. In recent years, the research of ultrasound in the fields of biology and chemistry is very popular. Ultrasound has good applications in various fields du...

Examples

example 1

[0034]Weigh 0.04g enzymatically soluble collagen (collagen is prepared by the laboratory, chicken feet skin is removed by a solution of 20% (w / v) NaCl (0.05mol / L Tris-HCl, pH7.5) at 4°C Impurities such as miscellaneous proteins, fats and pigments. Pretreated chicken paw skin is extracted with precooled 0.5M acetic acid containing 0.1% (w / v) pepsin (2500U / mg), centrifuged, and the supernatant salting out gained precipitation is vacuum Freeze-drying is enzyme-soluble collagen.), with 0.5M acetic acid, prepared into a 0.02g / mL collagen solution, and dialyzed against phosphate buffer (200mM, pH7.4) in a refrigerator at 4°C. After the dialysis, the solution was centrifuged at 20000×g for 15 min, and the supernatant was taken. In a water bath at 30°C, use a single-frequency ultrasonic device at 270W / 60kHz for 5 minutes, and heat in a water bath at 30°C for 55 minutes to form collagen molecules and self-assemble to form a collagen gel. The collagen gel sponge was obtained by vacuum ...

example 2

[0037] Weigh 0.04g of enzyme-soluble collagen (made in the laboratory, same as Example 1), prepare 0.02g / mL collagen solution with 0.5M acetic acid, and dialyze against phosphate buffered saline (200mM, pH7.4) at 4°C . After the dialysis, the solution was centrifuged at 20000×g for 15 min, and the supernatant was taken. In a water bath at 30°C, use a single-frequency ultrasonic device at 270W / 60kHz for 5 minutes, and heat in a water bath at 30°C for 55 minutes to form collagen molecules and self-assemble to form a collagen gel. The collagen gel sponge was obtained by vacuum freeze-drying, which was used in the next step.

[0038] In the test, 2 mL of 0.02 g / mL collagen gel solution was prepared with distilled water to simulate gastrointestinal digestion in vitro. The bionic dynamic mouse stomach digestion system is set with an external electric compression device, which is adjusted by the motor to be close to the real mouse stomach peristaltic contraction frequency, that is,...

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