Molecular marker method of beef cattle fat color trait

Abstract

The invention discloses a molecular marker method of a beef cattle fat color trait. The method comprises steps as follows: DNA extraction of bovine genome, design of a third exon primer of a BCO2 (beta-carotene oxygenase 2) gene, in-vitro amplification and genotype detection. The method can be used for assistant selection of the fat color trait in cattle breeding and can realize early selection of breeder cattle; with the adoption of the gene pyramiding method, the good genotype of the BCO2 gene can be stabilized after one generation, that is, homozygosis of a good gene of the fat color trait of a beef cattle strain is realized through selection of one generation, the generation interval is greatly shortened, and the selection process is accelerated. The molecular marker method is simple and convenient to operate, condition requirements in the polymerase chain reaction process are low, the length of amplified fragments is short (525bp), amplification is easier, and the amplification efficiency and the judgement accuracy of the genotype are improved.

Description

technical field

[0001] The invention relates to a molecular marking method for the color properties of beef cattle fat. Background technique

[0002] Fat color is one of the most intuitive indicators of high-grade beef, and it is also the most important part of beef grading in various countries. In 1995, Hayes reported in Australia that yellow fat in exported carcasses would reduce by 10%. If there was 50% yellow fat, it would reduce the income of the Australian beef cattle industry by 9.2 million US dollars every year; my country Liu Xiaomu and others slaughtered in Shandong Province According to the survey of the enterprise, the price of yellow fat meat is about 40 yuan lower than that of white fat meat per kilogram of high-grade beef. According to relevant reports, beef yellow fat is mainly caused by β-carotenoids in adipose tissue. Carotenoids are a group of chemical compounds found in plants, and the dominant ones in fat are β-carotene and leaf carotene. Flavin, carote...

Examples

Embodiment Construction

[0037] In order to realize the detection of the third exon mutant of beef cattle β-carotene 9', 10' dioxygenase (BCO2) gene, this embodiment provides a molecular marker method for beef fat color traits.

[0038] In this example, primers for the third exon of the beef cattle BCO2 gene were designed, a detection method for the w80x site mutant of the third exon was established, and the correlation between its polymorphism and fat color traits was analyzed, and the The excellent genotype that affects the fat color is used for the selection of the fat color traits of beef cattle, which solves the problems of large blindness and low selection accuracy of traditional seed selection methods.

[0039] The specific operation steps of the molecular marker method for the color traits of beef cattle fat in this embodiment are as follows:

[0040] 1. Extraction of bovine genomic DNA;

[0041] 2, β-carotene 9', 10' dioxygenase (BCO2) gene 3rd exon primer design, described primer is upstrea...

technical field

[0001] The invention relates to a molecular marking method for the color properties of beef cattle fat. Background technique

[0002] Fat color is one of the most intuitive indicators of high-grade beef, and it is also the most important part of beef grading in various countries. In 1995, Hayes reported in Australia that yellow fat in exported carcasses would reduce by 10%. If there was 50% yellow fat, it would reduce the income of the Australian beef cattle industry by 9.2 million US dollars every year; my country Liu Xiaomu and others slaughtered in Shandong Province According to the survey of the enterprise, the price of yellow fat meat is about 40 yuan lower than that of white fat meat per kilogram of high-grade beef. According to relevant reports, beef yellow fat is mainly caused by β-carotenoids in adipose tissue. Carotenoids are a group of chemical compounds found in plants, and the dominant ones in fat are β-carotene and leaf carotene. Flavin, carote...

Examples

Embodiment Construction

[0037] In order to realize the detection of the third exon mutant of beef cattle β-carotene 9', 10' dioxygenase (BCO2) gene, this embodiment provides a molecular marker method for beef fat color traits.

[0038] In this example, primers for the third exon of the beef cattle BCO2 gene were designed, a detection method for the w80x site mutant of the third exon was established, and the correlation between its polymorphism and fat color traits was analyzed, and the The excellent genotype that affects the fat color is used for the selection of the fat color traits of beef cattle, which solves the problems of large blindness and low selection accuracy of traditional seed selection methods.

[0039] The specific operation steps of the molecular marker method for the color traits of beef cattle fat in this embodiment are as follows:

[0040] 1. Extraction of bovine genomic DNA;

[0041] 2, β-carotene 9', 10' dioxygenase (BCO2) gene 3rd exon primer design, described primer is upstrea...

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