Experimental method for promoting tissue engineering pre-vascularization by umbilical cord mesenchymal stem cells

A technology of stem cells and tissue engineering, applied in the medical field, can solve problems such as unclear

CN107748260AInactive Publication Date: 2018-03-02XIAN TISSUE ENG & REGENERATIVE MEDICINE RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2018-03-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an experimental method for promoting tissue engineering pre-vascularization by umbilical cord mesenchymal stem cells; according to the method, vascular endothelial cells and umbilical cord mesenchymal stem cells with different proportions are co-cultured in a two-dimensional manner, and a rule of promoting vascular endothelial cell proliferation and hematopoietic differentiation by the umbilical cord mesenchymal stem cells is studied. In addition, a micron-sized network microvascular scaffold is prepared by high temperature molten monosaccharide and is dissolved in hydrogel to construct a continuous microchannel culture system of a three-dimensional hydrogel female die, the two cells are subjected to perfusion and suspension culture, an in-vitro constructed microvascular network structure and an engineered bone tissue in-vivo transplantation effect based on the structure are evaluated, and a mechanism of the two cells participating and promoting microvascular network construction is illuminated. A new solution and an experimental basis are provided for in-vitro pre-vascularization of engineered tissues and organs.
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Description

technical field

[0001] The invention belongs to the technical field of medicine, and relates to an experimental method for promoting prevascularization of tissue engineering by umbilical cord mesenchymal stem cells. Background technique

[0002] In vitro prevascularization is crucial for successful in vivo transplantation of engineered tissues and organs. Some progress has been made in angiogenesis based on vascular endothelial cells and bioscaffold materials, but how to construct a continuous and stable three-dimensional network microvascular system in vitro is still unclear. Recently, studies have found that umbilical cord mesenchymal stem cells have multilineage differentiation potential and can secrete a variety of cytokines that promote proliferation and differentiation. Our previous work showed that umbilical cord mesenchymal stem cells can participate in and promote the proliferation and angiogenesis differentiation of vascular endothelial cells. Contents of the in...

Examples

Embodiment

[0142] 1. Primary cell culture of umbilical cord mesenchymal stem cells. Such as figure 2 shown.

[0143] 2. Primary culture of umbilical cord vein endothelial cells. Such as image 3 shown.

[0144] 3. Detection of surface markers of umbilical cord mesenchymal stem cells. Such as Figure 4-Figure 10 shown.

[0145] 4. Detection of osteogenic differentiation of umbilical cord mesenchymal stem cells. Such as Figure 11 shown.

[0146] 5. Detection of adipogenic differentiation of umbilical cord mesenchymal stem cells. Such as Figure 12 shown.

[0147] 6. Staining of the colony formation rate of umbilical cord mesenchymal stem cells. Such as Figure 13 shown.

[0148] 7. The umbilical cord vein endothelial cells positively expressed CD-31. Such as Figure 14 shown.

[0149] 8. The umbilical cord vein endothelial cells positively expressed VEGF. Such as Figure 15 shown.

[0150] 9. Umbilical cord vein endothelial cells positively expressed factor Ⅷ. Such as ...