A kind of infectious spleen and kidney necrosis virus orf022 gene deletion strain and its preparation method and application

A technology of ORF022 and spleen-kidney necrosis virus, applied in biochemical equipment and methods, viruses, viral peptides, etc., can solve problems that have not been reported in the research, and achieve the effects of high immunization rate, cost saving, and convenient immunization methods

CN108220252BActive Publication Date: 2022-02-11SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2022-02-11

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Abstract

The invention provides an infectious spleen-kidney necrosis virus ORF022 gene deletion strain and its preparation method and application, which is an attenuated virus strain prepared by deleting the ORF022 gene of the infectious spleen-kidney necrosis virus strain. The present invention also provides an infectious spleen and kidney necrosis virus ORF022 gene deletion strain with a screening marker and its preparation method and application, wherein the ORF022 gene is deleted for the infectious spleen and kidney necrosis virus, and homologous recombination is carried out at the gene deletion site An attenuated virus strain made by inserting a selection marker gene. The infectious spleen-kidney necrosis virus ORF022 gene deletion strain provided by the present invention is a recombinant genetically engineered vaccine in which the ORF022 gene has been knocked out. Immunity effect, without injection, great application value.
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Description

technical field

[0001] The invention relates to the field of prevention and control of aquaculture diseases, in particular to an infectious spleen and kidney necrosis virus ORF022 gene deletion strain and its preparation method and application. Background technique

[0002] Infectious spleen-kidney necrosis virus (ISKNV) is a representative species of the genus Dymovirus. ISKNV causes infectious diseases after infecting the main host mandarin fish, and causes a large number of mandarin fish to die. The group nucleic acid sequence of the whole ISKNV gene has been determined, its full length is 111362 bp, the G+C content is 54.78%, it contains 124 potential open reading frames (ORF), and the coding length ranges from 40 to 1208 amino acids. Through sequence analysis, it is speculated that ORF006L is the main capsid protein; ORF19R, ORF46L, ORF27L and ORF63L are related to DNA replication, modification and processing; ORF24, ORF28L, ORF34R, ORF64L and ORF87R are related to tra...

Examples

Embodiment Construction

[0052] The following description is a preferred embodiment of the present invention, it should be pointed out that for those skilled in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications are also considered Be the protection scope of the present invention.

[0053] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The experimental method that does not indicate specific condition in the embodiment, generally according to routine condition. Unless otherwise specified in the examples of the present invention, all reagents and consumables used are commercially available.

[0054] The embodiment of the present invention provides a preparation method of recombinant virus vaccine with ORF022 gene deletion of infectious spleen and kidney necrosis virus, comprising the following steps:

[00...