Quantum dot immunochromatography detection card and detection method for detection of oxaprozine by double antibody sandwich immunocompetitive method

A double-antibody sandwich and oxaprozine technology, which is applied in measurement devices, analytical materials, material excitation analysis, etc., can solve the problems of ununiform preparation of signal substances, low detection signal strength and sensitivity, and insufficient stability.

CN108507989BActive Publication Date: 2021-10-26SHAOGUAN COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2021-10-26

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Abstract

The invention relates to a quantum dot immunochromatographic detection card and a detection method for detecting oxaprozine by a double-antibody sandwich immunocompetitive method. The detection card includes a liner, a detection antigen release pad, a quantum dot probe release pad, a chromatographic membrane and a water-absorbent pad; the detection antigen release pad contains a detection antigen coupled by ovalbumin and oxaprozine; The dot probe release pad contains quantum dot probes marked with anti-ovalbumin antibody; the chromatographic membrane is provided with a detection line and a quality control line, the detection line is fixed with anti-oxaprozin antibody, and the quality control line is fixed with ovalbumin antigen. The detection antigen dissolves into the detection system from the release pad, forms a double-antibody sandwich immune complex with the detection antibody and the labeled antibody, and generates a fluorescent detection signal. Under the action of the detection reference of the quality control line, it is judged whether the sample contains oxaprozine according to the fluorescent signal on the detection line, which increases the signal strength and stability of the detection, and improves the detection sensitivity and quantitative precision.
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Description

technical field

[0001] The invention relates to the field of food and drug safety detection, in particular to a method of applying immunochemical detection technology to the detection of illegally added chemicals in health products and Chinese patent medicines, especially the quantum dot immune layer of oxaprozine detected by the double-antibody sandwich immunocompetition method Analysis test card and test method. Background technique

[0002] The chemical name of oxaprozine is 2-(-4-isobutylphenyl)propionic acid, which belongs to non-steroidal anti-inflammatory drugs, has anti-inflammatory, analgesic and antipyretic effects, and is used for rheumatoid arthritis, adhesion spondylitis, non-inflammatory joint pain, arthritis, non-articular rheumatism, pain caused by non-articular inflammation, various neuralgia, cancer pain, post-traumatic pain and fever caused by various inflammations, etc., but there are certain It is a prescription drug clinically and must be taken under t...

Examples

Embodiment Construction

[0029] see figure 1 , which is the quantum dot immunochromatography detection card for detecting oxaprozine by the double-antibody sandwich immunocompetitive method of this embodiment, including a PVC liner, and sequentially adhered to the PVC liner and partially overlapped between adjacent ones. Detect antigen release pad, quantum dot probe release pad, nitrocellulose membrane (NC membrane) and water-absorbing pad; Described detection antigen release pad comprises the detection antigen that is formed by coupling of OVA and oxaprozine; Said quantum dot The probe release pad contains quantum dot probes labeled with anti-OVA antibodies; the nitrocellulose membrane is provided with detection lines and quality control lines, the detection lines are immobilized with anti-oxaprozin antibodies, and the quality control lines are immobilized with OVA antigen.

[0030] Specifically, the preparation method of the detection antigen release pad is as follows: a glass cellulose membrane wi...