Diclofenac quantum dot immunochromatography detection card and detection method based on signal amplification system

A technology for diclofenac and signal amplification, which is applied in measuring devices, analytical materials, biological testing, etc., can solve problems such as insufficient stability, low detection signal strength and sensitivity, and inability to uniformly prepare signal substances, so as to achieve active protection and detection The effect of increasing the stability of the antibody in a short period of time

CN108267603APending Publication Date: 2018-07-10SHAOGUAN COLLEGE
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2018-07-10

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Abstract

The invention relates to a diclofenac quantum dot immunochromatography detection card and detection method based on a signal amplification system. The detection card comprises a lining plate, a detection antigen release pad, a quantum dot probe release pad, a chromatography film and a water absorption pad, wherein the detection antigen release pad contains detection antigens formed by coupling ovalbumin and diclofenac; the quantum dot probe release pad comprises a quantum dot probe labeled with anti-ovalbumin antibodies; the chromatography film is provided with a detection line and a quality control line; the detection line is fixed with anti-diclofenac antibodies; the quality control line is fixed with ovalbumin antigens; the detection antigens are dissolved into the detection system fromthe release pad; a ternary system immune complex is formed with the detection antibodies and marking antibodies; fluorescence detection signals are generated. Under the detection reference effects ofthe quality control line, whether the sample contains the diclofenac or not is judged according to the fluorescence signal on the detection line; the detected signal intensity and stability can be enhanced; the detection sensitivity and the quantity precision are improved.
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Description

technical field

[0001] The present invention relates to the field of food and drug safety detection, in particular to a method of applying immunochemical detection technology to the detection of illegally added chemicals in health care products and Chinese patent medicines, especially a diclofenac quantum dot immunochromatographic detection card and detection method based on a signal amplification system . Background technique

[0002] The chemical name of diclofenac is 2-[(2,6-dichloro-3-methylphenyl)amino]benzoic acid, which belongs to non-steroidal anti-inflammatory drugs, has anti-inflammatory, analgesic and antipyretic effects, and is used for rheumatism Arthritis, adhesive spondylitis, non-inflammatory joint pain, arthritis, non-articular rheumatism, pain caused by non-articular inflammation, various neuralgia, cancer pain, post-traumatic pain and fever caused by various inflammations etc., but there are certain side effects, which are clinically prescribed drugs and ...

Examples

Embodiment Construction

[0029] see figure 1 , which is the diclofenac quantum dot immunochromatography detection card based on the signal amplification system of this embodiment, including a PVC liner, and detection antigen release pads that are sequentially adhered to the PVC liner and partially overlapped between adjacent ones, quantum dots Point probe release pad, nitrocellulose membrane (NC membrane) and water-absorbing pad; The detection antigen release pad comprises the detection antigen formed by OVA and diclofenac coupling; The quantum dot probe release pad contains anti-OVA Antibody quantum dot probe; the nitrocellulose membrane is provided with a detection line and a quality control line, the detection line is fixed with anti-diclofenac antibody, and the quality control line is fixed with OVA antigen.

[0030] Specifically, the preparation method of the detection antigen release pad is as follows: a glass cellulose membrane with a thickness of 0.85 mm is used to fully absorb the reagent con...