EGCG chitosan/beta-lactoglobulin composite nanoparticles and preparation method thereof

Abstract

The invention discloses an EGCG chitosan / beta-lactoglobulin composite nanoparticle. The EGCG chitosan / beta-lactoglobulin composite nanoparticle is characterized by being prepared from EGCG, chitosan and beta-lactoglobulin at the mass ratio of 1 to (3-6) to (1-3); the chitosan is selected from a mixture of carboxymethyl chitosan and chitosan hydrochloride at the mass ratio of 1 to (1-1.5); the particle size range of the composite nanoparticle is 100-300nm; and Zeta potential is 20-30mV. The chitosan / beta-lactoglobulin composite nanoparticle coated with the EGCG is prepared from the carboxymethyl chitosan, the chitosan hydrochloride and the beta-lactoglobulin. A core-shell composite nanoparticle structure for transporting the EGCG is formed by crosslinking and assembling of the beta-lactoglobulin and the chitosan subjected to heat treatment. The chitosan / beta-lactoglobulin composite nanoparticle is capable of avoiding degradation of acid and enzyme in a body, has high stability, and has certain targeting and slow-release properties; the problem that an existing chitosan-sodium tripolyphosphate nanoparticle is easily degraded by gastric acid as an oral delivery carrier is solved; and the bioavailable efficiency of oral absorption of the EGCG is improved.

Description

technical field

[0001] The patent of the present invention relates to an EGCG chitosan / β-lactoglobulin composite nanoparticle that can be used in the food industry and a preparation method thereof, and belongs to the technical field of tea and food deep processing. Background technique

[0002] Epigallocatechin gallate (EGCG) is an important catechin component with high content in green tea polyphenols. Because of its outstanding biological activity such as antioxidant, it is widely used in the field of food industry. However, EGCG has poor oral absorption and low in vivo bioavailability, requiring a good delivery vehicle to enhance its effective intestinal absorption. Chitosan has good biocompatibility and biodegradability, making it very suitable as a good delivery vehicle for food nutrients. Chitosan nanocarriers can improve the bioavailability of nutrients by adjusting the release rate of the embedded nutrients, so that they can be released slowly within a predetermine...

Examples

Embodiment Construction

[0018] 1. Weigh a certain amount of carboxymethyl chitosan and chitosan hydrochloride with deionized water to prepare an aqueous solution with a concentration of 1-2%, and the mass ratio of the two chitosans is: 1:1 -1.5. Two kinds of chitosan (carboxymethyl chitosan and chitosan hydrochloride) were purchased from Jinan Haidebei Marine Biological Engineering Co., Ltd. Among them, the molecular weight of carboxymethyl chitosan is 60kDa, and the degree of deacetylation is 80%, and the molecular weight of chitosan hydrochloride is 90kDa, and the degree of deacetylation is 85%.

[0019] 2. Add a certain amount of β-lactoglobulin and EGCG to chitosan hydrochloric acid according to the ratio of EGCG, the total amount of chitosan and the mass ratio of β-lactoglobulin to 1:3-6:1-3. In the salt solution and stirring evenly at room temperature (rotation speed is 500-800rpm, time is 20-30min). Among them, β-lactoglobulin (purity of 90%) was purchased from Shanghai Huayi Biotechnology C...

technical field

[0001] The patent of the present invention relates to an EGCG chitosan / β-lactoglobulin composite nanoparticle that can be used in the food industry and a preparation method thereof, and belongs to the technical field of tea and food deep processing. Background technique

[0002] Epigallocatechin gallate (EGCG) is an important catechin component with high content in green tea polyphenols. Because of its outstanding biological activity such as antioxidant, it is widely used in the field of food industry. However, EGCG has poor oral absorption and low in vivo bioavailability, requiring a good delivery vehicle to enhance its effective intestinal absorption. Chitosan has good biocompatibility and biodegradability, making it very suitable as a good delivery vehicle for food nutrients. Chitosan nanocarriers can improve the bioavailability of nutrients by adjusting the release rate of the embedded nutrients, so that they can be released slowly within a predetermine...

Examples

Embodiment Construction

[0018] 1. Weigh a certain amount of carboxymethyl chitosan and chitosan hydrochloride with deionized water to prepare an aqueous solution with a concentration of 1-2%, and the mass ratio of the two chitosans is: 1:1 -1.5. Two kinds of chitosan (carboxymethyl chitosan and chitosan hydrochloride) were purchased from Jinan Haidebei Marine Biological Engineering Co., Ltd. Among them, the molecular weight of carboxymethyl chitosan is 60kDa, and the degree of deacetylation is 80%, and the molecular weight of chitosan hydrochloride is 90kDa, and the degree of deacetylation is 85%.

[0019] 2. Add a certain amount of β-lactoglobulin and EGCG to chitosan hydrochloric acid according to the ratio of EGCG, the total amount of chitosan and the mass ratio of β-lactoglobulin to 1:3-6:1-3. In the salt solution and stirring evenly at room temperature (rotation speed is 500-800rpm, time is 20-30min). Among them, β-lactoglobulin (purity of 90%) was purchased from Shanghai Huayi Biotechnology C...

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