A kind of pulsatilla extract for treating arthritis externally and its preparation method

Abstract

The invention discloses a Pulsatilla pulsatilla extract for external use for treating arthritis and a preparation method thereof, comprising the following key steps: crushing and sieving the dried Pulsatillae medicinal material, adding a certain concentration of ethanol solution in proportion, and transferring it to a pressurized extractor, Heat preservation and pressure extraction for a certain period of time; after the extraction is complete, directly drop the extract into an appropriate amount of deionized water, and stir rapidly until there is precipitation, separate the precipitation, and dry to obtain the Pulsatilla pulsatilla extract. The present invention extracts and purifies Pulsatilla pulsatillae extract by using a pressurized-anti-solvent method, and the obtained Pulsatillae extract has a saponin content greater than 50%, and the particle size of the extract is about 220nm. It has good transdermal absorption performance for external use, compared to The Pulsatillae extract prepared by the traditional extraction method has the characteristics of high skin penetration rate and strong anti-inflammatory and analgesic effects. The invention provides a new high-safety administration route for the treatment of arthritis by the pulsatilla.

Description

Technical field:

[0001] The invention belongs to the field of preparation of extracts of traditional Chinese medicines, and in particular relates to a Pulsatilla pulsatilla extract for external use for treating arthritis and a preparation method thereof. Background technique:

[0002] Arthritis is a general term for inflammatory diseases that occur in joints and surrounding tissues and are caused by many factors such as inflammation, degeneration, and trauma. The pathogenesis is not clear. The clinical manifestations are redness, swelling, pain, joint dysfunction, joint deformity, etc., which seriously affect the quality of life of patients. Arthritis can occur in people of all ages, and the incidence rate of people over 50 years old is about 50%, while 90% of women and 80% of men over 65 years of age suffer from arthritis. Relevant data show that the total incidence of arthritis in my country is about 13%. As of 2015, at least 100 million arthritis patients in my country a...

Examples

Embodiment 1

[0045] Firstly, the dried Pulsatillae medicinal material was crushed through a 40-mesh sieve, and then soaked in 50% ethanol solution at a ratio of 1:10 for 30 minutes;

[0046] Then transfer to a pressurized extractor, pressurize to 0.5MPa with an air compressor, control the temperature at 110°C, keep warm and pressurized, and extract for 40min;

[0047] After the extraction is complete, the extract is taken out through the sampling tube, directly added dropwise to an appropriate amount of deionized water, and stirred rapidly. Among them, deionized water was used as the anti-solvent, the temperature was 20°C, the ratio of extract solution to deionized water was 1:3, and the stirring speed was 900r / min.

[0048] Stir until a precipitate precipitates, stop stirring, obtain a suspension, let it stand, then separate the precipitate, wash the precipitate three times with deionized water, and dry it into a dry powder to obtain the Pulsatilla pulsatilla extract.

Embodiment 2

[0050] Firstly, the dried Pulsatillae medicinal material was crushed through a 40-mesh sieve, and then soaked in 20% ethanol solution at a ratio of 1:30 for 30 minutes;

[0051] Then transfer to a pressurized extractor, pressurize to 0.4MPa with an air compressor, control the temperature at 100°C, keep warm and pressurized, and extract for 50min;

[0052] After the extraction is complete, the extract is taken out through the sampling tube, directly added dropwise to an appropriate amount of deionized water, and stirred rapidly. Among them, deionized water is used as the anti-solvent, the temperature is 30°C, the ratio of extract solution to deionized water is 1:7, and the stirring speed is 900r / min.

[0053] Stir until a precipitate precipitates, stop stirring, obtain a suspension, let it stand, then separate the precipitate, wash the precipitate three times with deionized water, and dry it into a dry powder to obtain the Pulsatilla pulsatilla extract.

Embodiment 3

[0055] Firstly, the dried Pulsatillae medicinal material was crushed through a 40-mesh sieve, and then soaked in 40% ethanol solution at a ratio of 1:20 for 30 minutes;

[0056] Then transfer to a pressurized extractor, pressurize to 0.4MPa with an air compressor, control the temperature at 120°C, keep warm and pressurized, and extract for 30 minutes;

[0057] After the extraction is complete, the extract is taken out through the sampling tube, directly added dropwise to an appropriate amount of deionized water, and stirred rapidly. Among them, deionized water was used as the anti-solvent, the temperature was 10°C, the ratio of extract solution to deionized water was 1:3, and the stirring speed was 1000r / min.

[0058] Stir until a precipitate precipitates, stop stirring, obtain a suspension, let it stand, then separate the precipitate, wash the precipitate three times with deionized water, and dry it into a dry powder to obtain the Pulsatilla pulsatilla extract.

Technical field:

[0001] The invention belongs to the field of preparation of extracts of traditional Chinese medicines, and in particular relates to a Pulsatilla pulsatilla extract for external use for treating arthritis and a preparation method thereof. Background technique:

[0002] Arthritis is a general term for inflammatory diseases that occur in joints and surrounding tissues and are caused by many factors such as inflammation, degeneration, and trauma. The pathogenesis is not clear. The clinical manifestations are redness, swelling, pain, joint dysfunction, joint deformity, etc., which seriously affect the quality of life of patients. Arthritis can occur in people of all ages, and the incidence rate of people over 50 years old is about 50%, while 90% of women and 80% of men over 65 years of age suffer from arthritis. Relevant data show that the total incidence of arthritis in my country is about 13%. As of 2015, at least 100 million arthritis patients in my country a...

Examples

Embodiment 1

[0045] Firstly, the dried Pulsatillae medicinal material was crushed through a 40-mesh sieve, and then soaked in 50% ethanol solution at a ratio of 1:10 for 30 minutes;

[0046] Then transfer to a pressurized extractor, pressurize to 0.5MPa with an air compressor, control the temperature at 110°C, keep warm and pressurized, and extract for 40min;

[0047] After the extraction is complete, the extract is taken out through the sampling tube, directly added dropwise to an appropriate amount of deionized water, and stirred rapidly. Among them, deionized water was used as the anti-solvent, the temperature was 20°C, the ratio of extract solution to deionized water was 1:3, and the stirring speed was 900r / min.

[0048] Stir until a precipitate precipitates, stop stirring, obtain a suspension, let it stand, then separate the precipitate, wash the precipitate three times with deionized water, and dry it into a dry powder to obtain the Pulsatilla pulsatilla extract.

Embodiment 2

[0050] Firstly, the dried Pulsatillae medicinal material was crushed through a 40-mesh sieve, and then soaked in 20% ethanol solution at a ratio of 1:30 for 30 minutes;

[0051] Then transfer to a pressurized extractor, pressurize to 0.4MPa with an air compressor, control the temperature at 100°C, keep warm and pressurized, and extract for 50min;

[0052] After the extraction is complete, the extract is taken out through the sampling tube, directly added dropwise to an appropriate amount of deionized water, and stirred rapidly. Among them, deionized water is used as the anti-solvent, the temperature is 30°C, the ratio of extract solution to deionized water is 1:7, and the stirring speed is 900r / min.

[0053] Stir until a precipitate precipitates, stop stirring, obtain a suspension, let it stand, then separate the precipitate, wash the precipitate three times with deionized water, and dry it into a dry powder to obtain the Pulsatilla pulsatilla extract.

Embodiment 3

[0055] Firstly, the dried Pulsatillae medicinal material was crushed through a 40-mesh sieve, and then soaked in 40% ethanol solution at a ratio of 1:20 for 30 minutes;

[0056] Then transfer to a pressurized extractor, pressurize to 0.4MPa with an air compressor, control the temperature at 120°C, keep warm and pressurized, and extract for 30 minutes;

[0057] After the extraction is complete, the extract is taken out through the sampling tube, directly added dropwise to an appropriate amount of deionized water, and stirred rapidly. Among them, deionized water was used as the anti-solvent, the temperature was 10°C, the ratio of extract solution to deionized water was 1:3, and the stirring speed was 1000r / min.

[0058] Stir until a precipitate precipitates, stop stirring, obtain a suspension, let it stand, then separate the precipitate, wash the precipitate three times with deionized water, and dry it into a dry powder to obtain the Pulsatilla pulsatilla extract.

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