An automated microcentrifuge tube capping device
Through the coordination of the ramp plate and the pressure rod, combined with the air-pressure cover and the glue liquid conversion sealing ring, the adhesive layer is formed by using the adhesive guide layer and the sealing guide layer, which solves the problem of insufficient sealing of the micro-centrifugal tube closure in the prior art. , improve the accuracy of experimental data and the efficiency of the cover.
Patent Information
- Application Number
- CN202010682302.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2020-07-15
- Publication Date
- 2024-12-31
- Estimated Expiration
- 2040-07-15
AI Technical Summary
The existing automated microcentrifugal tube cover closure device cannot achieve a comprehensive and uniform stress on the cover body, resulting in insufficient sealing and tightness after the cover, and there is a risk of sample leakage, affecting the experimental results and data accuracy.
The combination of a slope pressure plate and a pressure rod is used, combined with the air pressure cover and the glue liquid conversion sealing ring, through the action of the glue guide layer and the sealing guide layer, the glass glue is transported from the glue liquid conversion sealing ring to the glue sealing cartilage layer. Form a glue sealing layer to improve the sealing property after the cover is closed.
The sealing performance of the centrifugal tube after the cover is significantly improved, avoiding unexpected sample losses, improving the accuracy of experimental data and cover efficiency.
Smart Images

Figure CN111908393B_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of centrifuge tube capping, and more particularly to an automated micro centrifuge tube capping device. Background Art
[0002] Centrifuge tubes are commonly used in the field of medical testing. EP tubes (micro centrifuge tubes) are a type of centrifuge tube with a one-piece cap, which is used to seal the centrifuge tube to prevent the contents from leaking, being contaminated or volatilizing, and affecting subsequent experiments. In many daily laboratory operations, the lid needs to be opened or closed repeatedly due to numbering, sample addition, centrifugation and other operation steps. Currently, in most cases, this operation is performed manually. The lid is opened with the assistance of a lid opener, but there is no auxiliary device for closing the lid. The EP tube needs to be placed on a perforated plate and then closed with the thumb.
[0003] There are relatively few types of relatively automated capping devices currently available. One of them is to use magnetic force to close the cap by adding magnets to the EP tube mouth and the corresponding position of the pressure cap. In actual operation, since some experiments, some reagents and experimental methods require the use of magnetic force, this method has the risk of affecting the experimental results or increasing the limitations of the use of this centrifuge tube. At the same time, since the sample in the EP tube will vibrate and splash outward during centrifugation, there are extremely high requirements for the sealing of the centrifuge tube after the cap is closed, and there are also extremely high requirements for the tightness after the cap is closed. However, the existing automated capping method cannot achieve full and uniform force on the cap body when closing the cap, resulting in certain deficiencies in the sealing and tightness after the cap is closed, resulting in sample leakage during centrifugation. Summary of the invention
[0004] 1. Technical issues to be solved
[0005] In view of the problems existing in the prior art, the purpose of the present invention is to provide an automated micro centrifuge tube capping device, which can automatically complete the centrifuge tube capping process through the coordinated arrangement of an inclined pressure plate and a pressure bar, and significantly improve the efficiency of capping. At the same time, under the coordinated action of the pneumatic cap and the glue conversion sealing ring, the pressure bar can be refueled to fill the pneumatic cap for capping, and the pressure bar can be squeezed and ruptured in the process of closing the pneumatic cap. Without destroying the glue conversion sealing ring structure, the glass glue can be transferred from the glue conversion sealing ring to the glue sealing soft skeleton layer in cooperation with the glue guide layer and the closed guide layer, so that a glue sealing layer can be naturally formed at the connection between the tube body and the pneumatic cap when the cap is closed. Compared with the prior art, the sealing of the centrifuge tube after capping is significantly improved, effectively ensuring the smooth progress of the centrifugation process, effectively avoiding the accidental loss of samples during the centrifugation process, and improving the accuracy of the data.
[0006] 2. Technical solution
[0007] To solve the above problems, the present invention adopts the following technical solutions.
[0008] An automated micro centrifuge tube capping device comprises a device body, wherein the four corners of the lower end of the device body are fixedly connected to a bottom bracket, a conveyor belt braked by a motor and a gear is installed on the device body, two pairs of side brackets are installed on the upper end of the device body, and multiple groups of fixed points are arranged on the two pairs of side brackets, one group of fixed points on the pair of side brackets on the left side is connected to a pressure rod via an electric rotating shaft, and one group of fixed points on the side brackets on the right side is connected to an inclined pressure plate via a resistance rotating shaft, a plurality of evenly distributed hole grooves are arranged on the conveyor belt, centrifuge tubes are placed in the hole grooves, a collection box with a bottom and inner wall paved with rubber pads is placed on the left side of the device body, the centrifuge tube comprises a tube body and a pneumatic cover suspended on the tube body, an expansion air bag is fixedly inlaid on the upper end of the pneumatic cover, and a plurality of punching holes connected to the expansion air bag are drilled inside the pneumatic cover The impact channel is fixedly connected with a glue conversion sealing ring at the lower end of the pneumatic cover, and the impact channel is far away from the orifice of the expansion airbag and is located just above the cross-section of the glue conversion sealing ring. Through the coordinated arrangement of the inclined pressure plate and the pressure rod, the process of automatically completing the centrifuge tube capping can be realized, and the efficiency of capping can be significantly improved. At the same time, under the coordinated action of the pneumatic cover and the glue conversion sealing ring, the pressure rod is refueled to fill the pneumatic cover for capping, and the extrusion rupture can be carried out in the process of capping. Without destroying the structure of the glue conversion sealing ring, the glass glue is transferred from the glue conversion sealing ring to the glue sealing soft skeleton layer in cooperation with the glue guide layer and the closed guide layer, so that a glue sealing layer can be naturally formed at the connection between the tube body and the pneumatic cover when the cover is closed. Compared with the prior art, the sealing of the centrifuge tube after capping is significantly improved, the smooth progress of the centrifugation process is effectively guaranteed, the accidental loss of samples during the centrifugation process is effectively avoided, and the accuracy of the data is improved.
[0009] Furthermore, the multiple groups of fixed points on the two pairs of side brackets correspond to each other, the fixed points on the left side bracket are 5-15mm higher than the corresponding fixed points on the right side bracket, and the fixed points where the inclined pressure plate and the pressure bar are located correspond to each other, and there is a certain height difference, so that when the unclosed centrifuge tube passes through the inclined pressure plate, the air-pressure type cover is initially pressed at a certain acute angle to the mouth of the tube body, so that the air-pressure type cover at the lower part is lower than the pressure bar, so that when it runs to the pressure bar, it is convenient for it to enter under the pressure bar, thereby facilitating closing the cover, and effectively avoiding the situation where the air-pressure type cover in the initial pressure state falls off from the tube body due to the squeezing effect of the pressure bar.
[0010] Furthermore, the glue conversion sealing ring is filled with glass glue, and a pre-broken film layer is fixedly connected to the notch below the impact channel. When the cover is not closed, the glass glue can be sealed in the glue conversion sealing ring.
[0011] Furthermore, the elastic film material is made of uneven thickness. When closing the cover, the expansion airbag is squeezed by the pressure rod, and the gas inside it will move to the impact channel and generate impact force. Due to the uneven thickness, the thinner part on it will be squeezed and broken under the impact force. At this time, due to the squeezing effect of the pressure rod, the glue conversion sealing ring is squeezed and contacted with the upper edge of the tube body, thereby causing the glue conversion sealing ring to be compressed, so that the glass glue inside it passes through the rupture on it and enters the expansion airbag, and spreads downward along the glue conductive layer. After overflowing from the glue conductive layer, it can flow to the outer surface of the tube body and the connection of the glue conversion sealing ring, thereby naturally forming a layer of glue sealing layer at the connection. Compared with the existing technology, the sealing performance of the centrifuge tube after closing the cover is significantly improved, effectively ensuring the smooth progress of the centrifugation process, effectively avoiding the accidental loss of samples during centrifugation, and improving the accuracy of the data.
[0012] Furthermore, a rubber conductive layer is provided on the outside of the impact channel, a closed guide layer is provided at the lower end of the rubber conductive layer, the rubber conductive layer is a multi-permeable hole structure, and the rubber conductive layer is embedded in the air pressure type cover, and the end of the rubber conductive layer away from the impact channel is located outside the glue conversion sealing ring.
[0013] Furthermore, a glue sealing soft skeleton layer is provided on the outer surface of the glue conversion sealing ring.
[0014] Furthermore, the glue-sealed soft skeleton layer is a woven mesh structure with multiple protrusions, which can effectively improve the adhesion of the glue conversion sealing ring surface to the glass glue, thereby effectively ensuring that when the glass glue enters the glue-sealed soft skeleton layer through the closed guide layer, it can penetrate into the mesh gaps of the glue-sealed soft skeleton layer, thereby improving the protective strength of the formed glue-sealed layer for the connection, and is not prone to loosening or even falling off at the connection with the tube body and the pneumatic cover due to high-speed rotation vibration during centrifugation. The closed guide layer extends into the glue-sealed soft skeleton layer, and the closed guide layer facilitates better centralized guidance of the glass glue to the glue-sealed soft skeleton layer, so that the formed glue-sealed layer has better sealing performance.
[0015] Furthermore, the closed guide layer includes a solidifying sealing layer embedded in the end of the conductive glue layer, and a plurality of evenly distributed hollow flexible conduits are embedded inside the solidifying sealing layer. The hollow flexible conduits penetrate the solidifying sealing layer, and one end of the hollow flexible conduit is embedded in the conductive glue layer, and the other end of the hollow flexible conduit is embedded in the glue-sealing soft skeleton layer. Due to the barrier of the solidifying sealing layer, the glass glue entering the conductive glue layer will not directly overflow from the end of the conductive glue layer, thereby effectively avoiding the overflowed glass glue from being too dispersed at the end of the conductive glue layer, resulting in an uneven glue sealing layer or even a situation where there are many gaps.
[0016] Furthermore, the lengths of the plurality of hollow flexible conduits are different, and the hollow flexible conduits closer to the outside are longer, so that the sealing layer formed by the glass glue guided by the hollow flexible conduits at the connection between the tube body and the air pressure cover has increasingly stronger sealing properties from the inside to the outside.
[0017] Furthermore, the interior of the expansion airbag is filled with compressed inert gas, and the compression ratio of the inert gas is 2:0.5-1. The inert gas in the compressed state makes the impact channel more resistant to impact when squeezed, so that it can be broken in time during the closing process.
[0018] 3. Beneficial effects
[0019] Compared with the prior art, the advantages of the present invention are:
[0020] (1) This solution can automatically complete the process of closing the centrifuge tube cap by coordinating the inclined pressure plate and the pressure rod, significantly improving the efficiency of closing the cap. At the same time, under the cooperation of the air pressure type cap and the glue conversion sealing ring, the pressure rod can add oil to the air pressure type cap to close the cap, and the squeeze rupture can be removed. Without destroying the glue conversion sealing ring structure, the glass glue can be transferred from the glue conversion sealing ring to the glue sealing soft skeleton layer by coordinating the glue guide layer and the sealing guide layer. Therefore, a glue sealing layer can be naturally formed at the connection between the tube body and the air pressure type cap when closing the cap. Compared with the prior art, the sealing performance of the centrifuge tube after closing the cap is significantly improved, effectively ensuring the smooth progress of the centrifugation process, effectively avoiding the accidental loss of samples during the centrifugation process, and improving the accuracy of the data.
[0021] (2) The multiple groups of fixing points on the two pairs of side brackets correspond to each other. The fixing points on the left side bracket are 5-15mm higher than the corresponding fixing points on the right side bracket, and the fixing points where the inclined pressure plate and the pressure bar are located correspond to each other. There is a certain height difference, so that when the centrifuge tube that has not been closed passes through the inclined pressure plate, the air pressure type cover is initially pressed at a certain acute angle to the mouth of the tube body, so that the air pressure type cover at the lower part is lower than the pressure bar, so that when it runs to the pressure bar, it is convenient for it to enter under the pressure bar, so as to facilitate the closing of the cover, and effectively avoid the situation where the air pressure type cover in the initial pressure state falls off from the tube body due to the squeezing effect of the pressure bar.
[0022] (3) The glue conversion sealing ring is filled with glass glue, and is fixedly connected to the notch below the impact channel. When the cover is not closed, the glass glue can be sealed in the glue conversion sealing ring.
[0023] (4) It is made of an elastic film material with uneven thickness. When closing the cover, the expansion airbag is squeezed by the pressure rod, and the gas inside it will move toward the impact channel and generate impact force. Due to the uneven thickness, the thinner part on it will be squeezed and broken under the impact force. At this time, due to the squeezing effect of the pressure rod, the glue conversion sealing ring is squeezed and contacted with the upper edge of the tube body, thereby causing the glue conversion sealing ring to be compressed, so that the glass glue inside it passes through the rupture on the upper part and enters the expansion airbag, and spreads downward along the glue conductive layer. After overflowing from the glue conductive layer, it can flow to the outer surface of the tube body and the connection of the glue conversion sealing ring, thereby naturally forming a glue sealing layer at the connection. Compared with the existing technology, the sealing performance of the centrifuge tube after closing the cover is significantly improved, effectively ensuring the smooth progress of the centrifugation process, effectively avoiding the accidental loss of samples during the centrifugation process, and improving the accuracy of the data.
[0024] (5) A rubber conductive layer is provided on the outside of the impact channel, and a closed guide layer is provided at the lower end of the rubber conductive layer. The rubber conductive layer is a multi-permeable hole structure, and the rubber conductive layer is embedded in the air pressure cover. The end of the rubber conductive layer away from the impact channel is located on the outside of the glue liquid conversion sealing ring.
[0025] (6) A glue-sealing soft skeleton layer is provided on the outer surface of the glue conversion sealing ring. The glue-sealing soft skeleton layer is a woven mesh structure with multiple protrusions, which can effectively improve the adhesion of the surface of the glue conversion sealing ring to the glass glue, thereby effectively ensuring that when the glass glue enters the glue-sealing soft skeleton layer through the closed guide layer, it can penetrate into the mesh gaps of the glue-sealing soft skeleton layer, thereby improving the protective strength of the formed glue-sealing layer for the connection, and is not easy to loosen or even fall off at the connection with the tube body and the air pressure cover due to high-speed rotation vibration during centrifugation. The closed guide layer extends into the glue-sealing soft skeleton layer, and the closed guide layer facilitates better concentration and guidance of the glass glue to the glue-sealing soft skeleton layer, so that the formed glue-sealing layer has better sealing performance.
[0026] (7) The closed guide layer includes a solidified sealing layer embedded in the end of the conductive glue layer, and a plurality of evenly distributed hollow flexible conduits are embedded inside the solidified sealing layer. The hollow flexible conduits penetrate the solidified sealing layer, and one end of the hollow flexible conduits is embedded in the conductive glue layer, and the other end of the hollow flexible conduits is embedded in the glue-sealing soft skeleton layer. Due to the barrier of the solidified sealing layer, the glass glue entering the conductive glue layer will not directly overflow from the end of the conductive glue layer, thereby effectively preventing the overflowed glass glue from being too dispersed at the end of the conductive glue layer, resulting in an uneven glue sealing layer or even a situation where there are many gaps.
[0027] (8) The lengths of the plurality of hollow flexible conduits are different, and the hollow flexible conduits closer to the outside are longer, so that the sealing layer formed by the glass glue guided by the hollow flexible conduits at the connection between the tube body and the air pressure cover has increasingly stronger sealing properties from the inside to the outside.
[0028] (9) The interior of the inflation airbag is filled with compressed inert gas, and the compression ratio of the inert gas is 2:0.5-1. The compressed inert gas makes the impact channel more resistant to impact when it is squeezed, so that it can be broken in time during the closing process. BRIEF DESCRIPTION OF THE DRAWINGS
[0029] Figure 1 It is a three-dimensional structural schematic diagram of the present invention;
[0030] Figure 2 It is a structural schematic diagram of the lid closing process of the present invention;
[0031] Figure 3 It is a schematic structural diagram of the air pressure type cover of the present invention;
[0032] Figure 4 It is a schematic structural diagram of the air pressure cover part of the present invention;
[0033] Figure 5 for Figure 4 The structural diagram at A in the middle;
[0034] Figure 6 It is a schematic structural diagram of the surface portion of the glue conversion sealing ring of the present invention;
[0035] Figure 7 It is a partial structural schematic diagram of the air pressure type cover and the end of the centrifuge tube after closing the cover of the present invention.
[0036] Description of the numbers in the figure:
[0037] 1 device body, 2 conveyor belt, 3 side bracket, 4 collection box, 5 inclined pressure plate, 6 pressure rod, 7 hole groove, 8 bottom bracket, 9 centrifuge tube, 91 tube body, 92 air pressure cover, 101 impact channel, 102 expansion airbag, 11 glue conversion sealing ring, 1101 glue sealing soft skeleton layer, 12 glue guide layer, 13 hollow flexible catheter, 14 curing sealing layer, 15 pre-breaking film layer. DETAILED DESCRIPTION
[0038] The following will combine the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all the embodiments. All other embodiments obtained by ordinary technicians in this field without creative work based on the embodiments of the present invention belong to the scope of protection of the present invention.
[0039] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top / bottom" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.
[0040] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "mounted / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0041] Embodiment 1:
[0042] See also Figure 1-2 , an automated micro centrifuge tube capping device, comprising a device body 1, the four corners of the lower end of the device body 1 are fixedly connected with a bottom bracket 8, a conveyor belt 2 braked by a motor and a gear is installed on the device body 1, two pairs of side brackets 3 are installed on the upper end of the device body 1, and multiple groups of fixed points are arranged on the two pairs of side brackets 3, one group of fixed points on the left side pair of side brackets 3 is connected with a pressure rod 6 through an electric shaft, and one group of fixed points on the right side bracket 3 is connected with an inclined pressure plate 5 through a resistance shaft, and a plurality of evenly distributed hole slots 7 are arranged on the conveyor belt 2, and centrifuge tubes 9 are placed in the hole slots 7. A collecting box 4 with a rubber pad on the bottom and inner wall is placed on the left side of the device body 1. The centrifuge tube 9 includes a tube body 91 and a gas pressure cover 92 suspended on the tube body 91. The multiple groups of fixed points on the two pairs of side brackets 3 correspond to each other. The fixed points on the left side bracket 3 are 5-15mm higher than the corresponding fixed points on the right side bracket 3. The fixed points of the inclined pressure plate 5 and the pressure bar 6 correspond to each other, and there is a certain height difference. When the centrifuge tube 9 that is not covered passes through the inclined pressure plate 5, the gas pressure cover 92 is initially pressed at a certain acute angle to the mouth of the tube body 91, so that the gas pressure cover 92 at a lower position is lower than the pressure bar 6. Therefore, when it runs to the pressure bar 6, it is convenient for it to enter under the pressure bar 6, so as to facilitate the closing of the cover, and effectively avoid the situation that the gas pressure cover 92 in the initial pressure state falls off from the tube body 91 due to the squeezing effect of the pressure bar 6.
[0043] See also Figure 3-5The upper end of the air pressure cover 92 is fixedly inlaid with an expansion airbag 102, and the expansion airbag 102 is filled with compressed inert gas. The compression ratio of the inert gas is 2:0.5-1. The inert gas in the compressed state makes the impact channel 101 stronger when it is squeezed. It is convenient for the pre-broken film layer 15 to be broken in time during the closing process. A plurality of impact channels 101 connected to the expansion airbag 102 are opened inside the air pressure cover 92. The lower end of the air pressure cover 92 is fixedly connected with a glue conversion sealing ring 11. The opening of the impact channel 101 away from the expansion airbag 102 is located directly above the cross section of the glue conversion sealing ring 11, and a glue conductive layer 12 is provided on the outer side of the impact channel 101;
[0044] See also Figure 4 and Figure 7 The glue conversion sealing ring 11 is filled with glass glue, and a pre-broken film layer 15 is fixedly connected to the notch below the impact channel 101. When the cover is not closed, the glass glue can be sealed in the glue conversion sealing ring 11 through the pre-broken film layer 15. The pre-broken film layer 15 is made of an elastic film material with uneven thickness. When the cover is closed, the inflatable airbag 102 is squeezed by the pressure rod 6, and the gas inside it will move toward the impact channel 101 and generate an impact force on the pre-broken film layer 15. Due to the uneven thickness of the pre-broken film layer 15, the thinner part on it will be squeezed and broken under the impact force. At this time, due to the squeezing effect of the pressure rod 6 , the glue conversion sealing ring 11 is squeezed and contacted with the upper edge of the tube body 91, so that the glue conversion sealing ring 11 is compressed, so that the glass glue inside it passes through the rupture on the pre-broken film layer 15, enters the expansion airbag 102, and spreads downward along the glue conductive layer 12. After overflowing from the glue conductive layer 12, it can flow to the outer surface of the tube body 91 and the connection of the glue conversion sealing ring 11, so that a glue sealing layer is naturally formed at the connection. Compared with the prior art, the sealing performance of the centrifuge tube 9 after closing the cover is significantly improved, the smooth progress of the centrifugation process is effectively guaranteed, the accidental loss of samples during the centrifugation process is effectively avoided, and the accuracy of the data is improved;
[0045] A closed guiding layer is provided at the lower end of the conductive rubber layer 12. The conductive rubber layer 12 is a multi-permeable hole structure, and the conductive rubber layer 12 is embedded in the air pressure cover 92. The end of the conductive rubber layer 12 away from the impact channel 101 is located outside the glue conversion sealing ring 11. The closed guiding layer includes a solidifying sealing layer 14 embedded in the end of the conductive rubber layer 12. A plurality of evenly distributed hollow flexible conduits 13 are embedded in the solidifying sealing layer 14. The hollow flexible conduits 13 penetrate the solidifying sealing layer 14, and one end of the hollow flexible conduit 13 is embedded in the conductive rubber layer 12, and the other end of the hollow flexible conduit 13 is embedded in the conductive rubber layer 12. In the sealing soft skeleton layer 1101, due to the obstruction of the solidified sealing layer 14, the glass glue entering the conductive glue layer 12 will not directly overflow from the end of the conductive glue layer 12, thereby effectively preventing the overflowed glass glue from being too dispersed at the end of the conductive glue layer 12, resulting in an uneven glue sealing layer or even a situation where there are many gaps. The lengths of the multiple hollow flexible conduits 13 are different, and the hollow flexible conduits 13 close to the outside are longer, so that the glass glue guided by the hollow flexible conduits 13 forms a glue sealing layer at the connection between the tube body 91 and the air pressure cover 92, and the sealing property becomes stronger and stronger from the inside to the outside.
[0046] See also Figure 6 The outer surface of the glue conversion sealing ring 11 is provided with a glue sealing soft skeleton layer 1101. The glue sealing soft skeleton layer 1101 is a woven mesh structure with multiple protrusions, which can effectively improve the adhesion of the surface of the glue conversion sealing ring 11 to the glass glue, thereby effectively ensuring that when the glass glue enters the glue sealing soft skeleton layer 1101 through the closed guide layer, it can penetrate into the mesh gap of the glue sealing soft skeleton layer 1101, thereby improving the protection strength of the formed glue sealing layer for the connection, and it is not easy to loosen or even fall off at the connection with the tube body 91 and the air pressure type cover 92 due to the high-speed rotation vibration during centrifugation. The closed guide layer extends into the glue sealing soft skeleton layer 1101, and the closed guide layer facilitates to better concentrate and guide the glass glue to the glue sealing soft skeleton layer 1101, so that the formed glue sealing layer has better sealing performance.
[0047] See also Figure 2 The specific steps of closing the cap of the microcentrifuge tube by the device are as follows:
[0048] Step 1: Turn on the power, turn on the device switch, make the motor work, and the conveyor belt moves at a constant speed;
[0049] Step 2: Place the centrifuge tubes 9 in the open state in batches on the hole slots 7. The centrifuge tubes 9 in the open state are uniformly transported to the vicinity of the inclined pressure plate 5 along the conveyor belt 2. When passing through the inclined pressure plate 5, the centrifuge tubes 9 in the open state are pressed by the inclined pressure plate 5 with an acute angle, so that the air pressure type cover 92 is initially pressed on the mouth of the tube body 91 with a certain acute angle.
[0050] Step 3: The centrifuge tube 9 in the initial pressure state is continuously conveyed by the conveyor belt to the pressure bar 6. Under the rolling pressure of the pressure bar 6, the centrifuge tube 9 is pressed into a closed cap state. Figure 7 At the same time, under the pressure of the pressure rod 6, a layer of glue sealing layer is formed at the edge of the centrifuge tube 9 after the cover is closed, so that the sealing performance of the centrifuge tube 9 after the cover is closed is significantly improved;
[0051] Step 4: The centrifuge tube 9 in the closed state continues to be transported by the conveyor belt to the leftmost end of the conveyor belt, and falls into the collection box 4 by itself.
[0052] By cooperating with the inclined pressure plate 5 and the pressure rod 6, the process of closing the centrifuge tube 9 can be automatically completed, and the efficiency of closing the cap can be significantly improved. At the same time, with the cooperation of the air pressure type cover 92 and the glue conversion sealing ring 11, the pressure rod 6 is filled with oil to close the air pressure type cover 92, and 15 can be squeezed and broken. Without destroying the structure of the glue conversion sealing ring 11, with the help of the glue guide layer 12 and the closed guide layer, the glass glue is transferred from the glue conversion sealing ring 11 to the glue sealing soft skeleton layer 1101, so that a layer of glue sealing can be naturally formed at the connection between the tube body 91 and the air pressure type cover 92 when closing the cap. Compared with the prior art, the sealing performance of the centrifuge tube 9 after closing the cap is significantly improved, which effectively ensures the smooth progress of the centrifugation process, effectively avoids the accidental loss of samples during the centrifugation process, and improves the accuracy of the data.
[0053] The above is only a preferred specific implementation manner of the present invention; but the protection scope of the present invention is not limited thereto; any technician familiar with the technical field within the technical scope disclosed by the present invention; any equivalent replacement or change based on the technical solution and improved concept of the present invention shall be covered by the protection scope of the present invention.
Claims
1. An automated microcentrifuge tube capping device, comprising a device body (1), wherein the four corners of the lower end of the device body (1) are fixedly connected to a bottom bracket (8), and a conveyor belt (2) braked by a motor and a gear is installed on the device body (1), characterized in that: Two pairs of side brackets (3) are installed at the upper end of the device body (1), and multiple groups of fixing points are arranged on the two pairs of side brackets (3). One group of fixing points on the left pair of side brackets (3) is connected to a pressure rod (6) via an electric shaft, and one group of fixing points on the right side bracket (3) is connected to an inclined pressure plate (5) via a resistance shaft. The conveyor belt (2) is provided with multiple evenly distributed hole grooves (7), and the hole grooves (7) are placed A centrifuge tube (9) is provided, and a collecting box (4) with a rubber pad on the bottom and inner wall is placed on the left side of the device body (1). The centrifuge tube (9) comprises a tube body (91) and a pneumatic cover (92) suspended on the tube body (91). An expansion air bag (102) is fixedly embedded on the upper end of the pneumatic cover (92). A plurality of impact channels (101) connected to the expansion air bag (102) are drilled inside the pneumatic cover (92). The lower end of the pneumatic cover (92) is fixedly embedded with a pneumatic cover (92). The side brackets (103) are fixedly connected with a glue conversion sealing ring (11), and the opening of the impact channel (101) away from the expansion airbag (102) is located directly above the cross section of the glue conversion sealing ring (11); the multiple groups of fixing points on the two pairs of side brackets (3) correspond to each other, and the fixing points on the left side bracket (3) are 5-15mm higher than the corresponding fixing points on the right side bracket (3), and the fixing points where the inclined pressure plate (5) and the pressure rod (6) are located correspond to each other; the glue conversion sealing ring (11) The interior is filled with glass glue, and a pre-broken film layer (15) is fixedly connected to the notch below the impact channel (101); a conductive glue layer (12) is provided on the outside of the impact channel (101), and a closed guide layer is provided at the end below the conductive glue layer (12). The conductive glue layer (12) is a multi-permeable hole structure, and the conductive glue layer (12) is embedded in the air pressure cover (92), and the end of the conductive glue layer (12) away from the impact channel (101) is located outside the glue conversion sealing ring (11).
2. The automated microcentrifuge tube capping device according to claim 1, characterized in that: The pre-broken film layer (15) is made of an elastic film material with uneven thickness.
3. The automated microcentrifuge tube capping device according to claim 2, characterized in that: The outer surface of the glue conversion sealing ring (11) is provided with a glue sealing soft skeleton layer (1101).
4. The automated microcentrifuge tube capping device according to claim 3, characterized in that: The glue-sealed soft skeleton layer (1101) is a woven mesh structure with multiple protrusions, and the closed guide layer extends into the glue-sealed soft skeleton layer (1101).
5. The automated microcentrifuge tube capping device according to claim 4, characterized in that: The closed guide layer comprises a solidifying sealing layer (14) embedded in the end of the conductive rubber layer (12), wherein a plurality of evenly distributed hollow flexible conduits (13) are embedded inside the solidifying sealing layer (14), wherein the hollow flexible conduits (13) penetrate the solidifying sealing layer (14), and one end of the hollow flexible conduit (13) is embedded in the conductive rubber layer (12), and the other end of the hollow flexible conduit (13) is embedded in the glue-sealed soft skeleton layer (1101).
6. The automated microcentrifuge tube capping device according to claim 5, characterized in that: The lengths of the plurality of hollow flexible conduits (13) are different, and the hollow flexible conduits (13) closer to the outside are longer.
7. An automated microcentrifuge tube capping device according to claim 6, characterized in that: The interior of the expansion airbag (102) is filled with compressed inert gas, and the compression ratio of the inert gas is 2:0.5-1.
