A surface plasmon resonance detection device

Abstract

The present invention discloses a surface plasmon resonance detection device, including a detection mechanism and an adjustment mechanism, the detection mechanism includes an incident component, an intermediate component and a receiving component, the intermediate component is located between the incident component and the receiving component, the intermediate component includes an optical coupling device, the incident component is used to generate linear polarized light incident to the optical coupling device, and the receiving component is used to receive and detect the linear polarized light refracted by the optical coupling device; the adjustment mechanism includes a first adjustment component and a second adjustment component, the first adjustment component is used to change the posture of the incident component to adjust the incident angle of the linear polarized light in the optical coupling device, and the second adjustment component is used to change the posture of the receiving component so that the receiving component can receive the linear polarized light refracted by the optical coupling device. The adjustment of the optical path is completed by changing the postures of the incident component and the receiving component, the requirement of total reflection of the linear polarized light at different measurement angles is met, and the operation steps are simplified.

Description

Technical Field

[0001] The invention is used in the technical field of optical instruments, and in particular relates to a surface plasma resonance detection device. Background Art

[0002] Surface plasmon resonance (SPR) technology is a technology that uses the physical optical phenomenon caused by the total reflection of light at the metal film / medium interface to analyze molecular interactions. It is a very sensitive optical analysis method. Surface plasmon resonance technology is widely used in monitoring fields such as food, medical care, and environment. Among them, the SPR detection principle is: when the incident angle θ is greater than the critical angle, p-polarized light parallel to the surface of the metal layer is emitted as the incident light, so that the p-polarized light passes through the prism and enters the metal layer. Since the p-polarized light passes through the prism and is totally reflected at the interface of the metal layer, the p-polarized light is refracted from the other side of the prism. At this time, under the condition of total reflection, the electric field at the metal surface and the prism interface does not disappear immediately, but transmits an exponentially decaying evanescent wave to the metal layer. The evanescent wave interacts with the free electrons in the metal layer, causing the free electrons in the metal layer to generate surface plasma. When the evanescent wave and the surface plasma have the same frequency and wave vector, the two will resonate. Due to the plasma resonance phenomenon, the total emission at the interface between the prism and the metal is destroyed, and the intensity of the reflected light reaches a minimum value. In actual SPR resonance detection, SPR angle and light intensity are used to characterize SPR measurement information. When surface plasmon resonance occurs, an obvious attenuation peak will appear in the SPR spectrum, and the lowest point of the peak is the SPR resonance angle.

[0003] In the prior art, the surface plasmon resonance detection device has the following disadvantages: First, it requires a high level of professional technical background to adjust the optical path, which is inconvenient to operate; second, the measurement angle is fixed, and only SPR information within a certain angle range can be measured. If you want to modify the measurement angle, you need to replace the mechanical rotating parts or the light intensity detector, and readjust the pipeline, which is extremely troublesome to operate and has low work efficiency. Summary of the invention

[0004] The purpose of the present invention is to solve at least one of the technical problems existing in the prior art and to provide a surface plasmon resonance detection device, which can change the posture of the incident component and the receiving component, facilitate the adjustment of the optical path, meet the requirements of total reflection of linearly polarized light at different measurement angles, simplify the operation steps and improve work efficiency.

[0005] The technical solution adopted by the present invention to solve its technical problem is:

[0006] A surface plasmon resonance detection device, comprising

[0007] A detection mechanism, comprising an incident component, an intermediate component and a receiving component, wherein the intermediate component is located between the incident component and the receiving component, the intermediate component comprises an optical coupling device, the incident component is used to generate linearly polarized light incident to the optical coupling device, and the receiving component is used to receive and detect the linearly polarized light refracted by the optical coupling device;

[0008] The adjustment mechanism includes a first adjustment component and a second adjustment component, wherein the first adjustment component is used to change the posture of the incident component to adjust the incident angle of the linearly polarized light in the optical coupling device, and the second adjustment component is used to change the posture of the receiving component so that the receiving component can receive the linearly polarized light refracted by the optical coupling device.

[0009] In combination with the above-mentioned implementations, in certain implementations of the present invention, the adjustment mechanism also includes a bracket, the intermediate component is installed on the bracket, the first adjustment component includes a first support seat, the incident component is installed on the first support seat, the second adjustment component includes a second support seat, the receiving component is installed on the second support seat, and the first support seat and the second support seat are both hinged to the bracket.

[0010] In combination with the above-mentioned implementation methods, in certain implementation methods of the present invention, a base is provided at the bottom of the bracket, and the first support seat and the second support seat are both provided with an adjustment hole and a first locking hole, and second locking holes are provided at both ends of the base. The first support seat and the second support seat are both connected to the base through a locking screw and an adjusting screw, and the locking screw passes through the first locking hole and then extends into the second locking hole, and the adjusting screw passes through the adjusting hole and then rests against the base.

[0011] In combination with the above implementations, in certain implementations of the present invention, the base is provided with mounting grooves on both sides of the bracket, an adjustment pad is provided in the mounting groove, the adjustment screw passes through the adjustment hole and rests against the adjustment pad, and the second locking hole is located on the adjustment pad.

[0012] In combination with the above-mentioned implementation methods, in certain implementation methods of the present invention, an incident fixing device is provided on the first support seat, and the incident fixing device is provided with an incident through hole for installing the incident component, and a receiving fixing device is provided on the second support seat, and the receiving fixing device is provided with a receiving through hole for installing the receiving component.

[0013] In combination with the above-mentioned implementation manner, in some implementation manners of the present invention, the incident component includes a light source, a first aperture, a first collimating lens, a polarizer, a second aperture and a focusing lens arranged in sequence along the light irradiation direction, the incident through hole includes a first incident hole and a second incident hole that are connected to each other, the light source and the first aperture are both installed in the first incident hole, an incident tube is provided in the second incident hole, and the first collimating lens, polarizer, second aperture and focusing lens are all located in the incident tube.

[0014] In combination with the above-mentioned implementation manner, in certain implementation manners of the present invention, the receiving component includes a second collimating lens, a first beam expanding lens, a second beam expanding lens, a cylindrical mirror and a light intensity detection device arranged in sequence along the light irradiation direction, the receiving through hole includes a first receiving through hole and a second receiving through hole that are connected to each other, a receiving tube is provided in the first receiving through hole, the second collimating lens, the first beam expanding lens, the second beam expanding lens and the cylindrical mirror are all located in the receiving tube, and the light intensity detection device is installed in the second receiving through hole.

[0015] In combination with the above-mentioned implementation manner, in certain implementation manners of the present invention, the receiving fixture is provided with a fixing groove that passes through the top of the receiving fixture, the fixing groove is located between the receiving tube and the light intensity detection device, an attenuation plate is provided in the fixing groove, a fixing seat is provided on the top of the attenuation plate, and the attenuation plate is connected to the receiving fixture via the fixing seat.

[0016] In combination with the above-mentioned implementations, in certain implementations of the present invention, the optical coupling device includes a lens, and the intermediate component also includes a lens mounting seat, the lens mounting seat is provided with a lens mounting groove for mounting the lens, the lens mounting seat is provided with openings on both sides of the lens mounting groove, the openings are connected to the lens mounting groove, and the lens mounting seat is provided with lens blocks at both ends of the lens mounting groove.

[0017] In combination with the above-mentioned implementation manner, in certain implementation manners of the present invention, the intermediate component also includes an intermediate fixing device, the intermediate fixing device having a first cavity and a second cavity that are connected to each other, the second cavity is located at the bottom of the first cavity, the first cavity is used to install the detection pool, the lens mount and the lens are both located in the second cavity and against the top of the second cavity, the intermediate fixing device is provided with a first connecting ear in front of the second cavity, and the intermediate fixing device is provided with a second connecting ear behind the second cavity, and the first connecting ear and the second connecting ear are both connected to the bracket.

[0018] One of the above technical solutions has at least one of the following advantages or beneficial effects: the surface plasmon resonance detection device of the technical solution changes the posture of the incident component through the first adjustment component to adjust the incident angle of the linear polarized light incident on the optical coupling device, so that the linear polarized light can be totally reflected after being incident on the optical coupling device; the posture of the receiving component is changed through the second adjustment component, so that the receiving component can receive the linear polarized light refracted by the optical coupling device and detect the linear polarized light. The adjustment of the optical path is completed by changing the postures of the incident component and the receiving component, which is convenient for adjusting the optical path, meets the requirement of total reflection of the linear polarized light at different measurement angles, simplifies the operation steps, and improves work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The present invention will be further described below in conjunction with the accompanying drawings:

[0020] Figure 1 is an assembly diagram of an embodiment of the present invention;

[0021] Figure 2 yes Figure 1 A schematic structural diagram of an embodiment shown;

[0022] Figure 3 yes Figure 1 A schematic diagram of the optical path principle of an embodiment shown

[0023] Figure 4 yes Figure 1 A schematic diagram of the structure of a middle component in one embodiment shown;

[0024] Figure 5 yes Figure 1 A schematic diagram of the connection between a lens, a lens mounting seat, a lens block and a detection cell according to an embodiment is shown. DETAILED DESCRIPTION

[0025] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present invention, but it cannot be understood as a limitation on the scope of protection of the present invention.

[0026] In the present invention, if directions (up, down, left, right, front and back) are described, it is only for the convenience of describing the technical solution of the present invention, and does not indicate or imply that the technical features referred to must have a specific orientation, be constructed and operate in a specific orientation. Therefore, it cannot be understood as a limitation of the present invention.

[0027] In the present invention, "several" means one or more, "multiple" means more than two, "greater than", "less than", "exceed" and the like are understood to exclude the number itself; "above", "below", "within" and the like are understood to include the number itself. In the description of the present invention, if there is a description of "first" or "second", it is only used for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0028] In the present invention, unless otherwise clearly defined, the words "set", "install", "connect" and the like should be understood in a broad sense, for example, they can be directly connected or indirectly connected through an intermediate medium; they can be fixedly connected or detachably connected or integrally formed; they can be mechanically connected or electrically connected or able to communicate with each other; they can be the internal connection of two elements or the interaction relationship between two elements. Those skilled in the art can reasonably determine the specific meanings of the above words in the present invention in combination with the specific content of the technical solution.

[0029] See also Figure 1 and Figure 2 The embodiment of the present invention provides a surface plasmon resonance detection device, including a detection mechanism and an adjustment mechanism, wherein the detection mechanism is used to detect the angle at which the surface plasmon resonates, and the adjustment mechanism is used to adjust the posture of the detection mechanism to achieve the purpose of adjusting the optical path. The detection mechanism includes an incident component 1, an intermediate component 2, and a receiving component 3, wherein the intermediate component 2 is located between the incident component 1 and the receiving component 3, and the intermediate component 2 includes an optical coupling device 21, and the incident component 1 is used to generate linearly polarized light with a measurement angle range incident on the optical coupling device 21. More specifically, the linearly polarized light is p-polarized light, that is, horizontally polarized light.

[0030] The receiving component 3 is used to receive and detect the linearly polarized light refracted by the optical coupling device 21. Figure 3 When linearly polarized light is incident on the optical coupling device 21 and is totally reflected at the metal layer 22 to form an evanescent wave, the free ions in the metal layer 22 interact with the evanescent wave, causing the free electrons in the metal layer 22 to generate surface plasma. When the evanescent wave resonates with the surface plasma, energy is transferred from the photon to the surface plasma, and most of the energy of the incident light is absorbed by the surface plasma, causing the energy of the refracted linearly polarized light to decrease sharply. Correspondingly, the reflected light intensity detected by the receiving component 3 will be greatly weakened, thereby determining the resonance angle of the linearly polarized light corresponding to the weakest reflected light intensity.

[0031] See also Figure 1 and Figure 2The adjustment mechanism includes a first adjustment component and a second adjustment component. The first adjustment component is used to change the posture of the incident component 1 to adjust the incident angle of the linear polarized light in the optical coupling device 21, so that the linear polarized light can be totally reflected after being incident on the optical coupling device 21, so as to induce the free electrons in the metal layer 22 to generate surface plasma. The second adjustment component is used to change the posture of the receiving component 3, so that the receiving component 3 can receive the linear polarized light refracted by the optical coupling device 21. The surface plasmon resonance detection device of the technical solution changes the posture of the incident component 1 by the first adjustment component, thereby adjusting the incident angle of the linear polarized light incident on the optical coupling device 21, so that it is totally reflected; and changes the posture of the receiving component 3 by the second adjustment component, so that the receiving component 3 can receive the linear polarized light refracted by the optical coupling device 21 and detect the linear polarized light. The adjustment of the optical path is completed by changing the postures of the incident component 1 and the receiving component 3, so that the linear polarized light can be totally reflected at different measurement angles, simplifying the operation steps, meeting the detection needs of the staff, and improving work efficiency.

[0032] It is understandable that the first adjustment component and the second adjustment component can be driven to rise and fall or rotate in a certain direction by means of a motor, a guide rail slider or an adjustment screw, so as to change the posture of the first adjustment component and the second adjustment component. Figure 1 and Figure 2 In some embodiments, the adjustment mechanism also includes a bracket 4, the intermediate component 2 is installed on the bracket 41, the first adjustment component includes a first support seat 42, the incident component 1 is installed on the first support seat 42, the second adjustment component includes a second support seat 43, and the receiving component 3 is installed on the second support seat 43. The first support seat 42 and the second support seat 43 are both hinged to the bracket 41, so that the first support seat 42 and the second support seat 43 can be rotated through the rotating shaft 44, thereby driving the incident component 1 and the receiving component 3 to rotate, so as to achieve the purpose of adjusting the optical path.

[0033] See also Figure 1 and Figure 2In some embodiments, a base 45 is provided at the bottom of the bracket 41, and an adjustment hole and a first locking hole are provided on the first support seat 42 and the second support seat 43. Second locking holes are provided at both ends of the base 45. The first support seat 42 and the second support seat 43 are connected to the base 45 through a locking screw 46 and an adjusting screw 47. The locking screw 46 passes through the first locking hole and then extends into the second locking hole. The adjusting screw 47 passes through the adjusting hole and then abuts against the base 45. After adjusting the angles of the first support seat 42 and the second support seat 43 respectively by the adjusting screw 47, the first support seat 42 and the base 45 are locked by the locking screw 46, and the second support seat 43 and the base 45 are locked, so as to achieve the purpose of adjusting the postures of the incident component 1 and the receiving component 3. By adjusting the posture of the incident component 1, the incident angle of the linear polarized light in the optical coupling device 21 is adjusted; by adjusting the posture of the receiving component 3, it is ensured that the receiving component 3 can receive the linear polarized light refracted by the optical coupling device 21.

[0034] See also Figure 1 and Figure 2 In some embodiments, the base 45 is provided with mounting grooves 48 on both sides of the bracket 41, and an adjusting pad 49 is provided in the mounting groove 48. The adjusting screw 47 passes through the adjusting hole and abuts against the adjusting pad 49, and the second locking hole is located on the adjusting pad 49. More specifically, the adjusting pad 49 has an inclined surface, and the adjusting screw 47 passes through the adjusting hole and abuts against the inclined surface of the adjusting pad 49. The setting of the adjusting pad 49 facilitates the installation and adjustment of the adjusting screw 47, and the operation is convenient. At the same time, by changing the adjusting pad 49 of different thicknesses, the problem that the adjusting screw 47 or the locking screw 46 is too short to meet the angle adjustment can be solved.

[0035] See also Figure 1 and Figure 2 In some embodiments, the first support seat 42 is provided with an incident fixture 5, and the incident fixture 5 is provided with an incident through hole for installing the incident component 1, and the second support seat 43 is provided with a receiving fixture 6, and the receiving fixture 6 is provided with a receiving through hole for installing the receiving component 3. The arrangement of the incident fixture 5 and the receiving fixture 6 facilitates the installation of the incident component 1 and the receiving component 3, simplifies the operation steps, and meets the use needs of the user.

[0036] The incident component 1 can filter and control the angle of the light, and then inject the linearly polarized light into the intermediate component 2. Figure 2 and 3In some embodiments, the incident component 1 includes a light source 11, a first aperture 12, a first collimating lens 13, a polarizer 14, a second aperture 15 and a focusing lens 16 arranged in sequence along the light irradiation direction, and the incident through hole includes a first incident hole and a second incident hole that are connected. The light source 11 and the first aperture 12 are both installed in the first incident hole. The light source 11 emits incident light, which becomes a point light source through the first aperture 12. An incident tube 17 is provided in the second incident hole, and the first collimating lens 13, the polarizer 14, the second aperture 15 and the focusing lens 16 are all located in the incident tube 17. The point light source 11 becomes parallel light after passing through the first collimating lens 13, and the parallel light emits horizontal polarized light after passing through the polarizer 14, and polarized light in other directions is filtered out. The horizontal polarized light retains the horizontal polarized light in a fixed angle range after passing through the second aperture 15, and then the horizontal polarized light is focused and converged by the focusing lens 16, so as to control the size of the area of ​​the SPR measurement region. The combination of the first aperture 12, the first collimating lens 13, the polarizer 14, the second aperture 15 and the focusing lens 16 can achieve polarization filtering and angle control of light. By providing the incident tube 17, it is convenient to replace different components inside the incident tube 17. Specifically, by replacing the second aperture 15 and / or the focusing lens 16, linear polarized light of different measurement angles can be generated, thereby achieving measurement and adjustment of different SPR measurement angles, reducing the maintenance difficulty and use difficulty of the device, and facilitating operation.

[0037] The optical coupling device 21 can be configured as a grating or a lens according to the detection requirements. Figure 2 , Figure 4 and Figure 5 In some embodiments, the optical coupling device 21 includes a lens. After the horizontal polarized light is focused and converged by the focusing lens 16, it is incident on the lens and forms a total reflection in the metal layer 22 on the top of the lens, and induces the free electrons in the metal layer 22 to form surface plasma. After the total reflection, the linear polarized light is refracted by the lens and refracted out at the same angle. The intermediate component 2 also includes a lens mounting seat 23. The lens mounting seat 23 is provided with a lens mounting groove for mounting the lens. The lens mounting seat 23 is provided with openings 24 on both sides of the lens mounting groove, and the openings 24 are connected to the lens mounting groove. The linear polarized light transmitted from the incident component 1 enters the lens through the opening 24 on one side of the lens mounting groove, and then enters the receiving component 3 after being transmitted out from the opening 24 on the other side of the lens mounting groove. The lens mounting seat 23 is provided with lens blocks 25 at both ends of the lens mounting groove. After the lens is placed in the lens mounting groove, the lens blocks 25 are installed to prevent the lens from falling.

[0038] In the plasma resonance detection technology, the metal layer 22 is usually connected to the detection cell 26, and the detection cell 26 can affect the surface plasma formed in the metal layer 22, thereby affecting the resonance angle corresponding to the resonance between the evanescent wave and the surface plasma. Figure 1 , Figure 2 , Figure 4 and Figure 5 In some embodiments, the intermediate component 2 further includes an intermediate fixing device 27, which has a first cavity and a second cavity connected to each other, the second cavity is located at the bottom of the first cavity, the first cavity is used to install the detection pool 26, the lens mounting seat 23 and the lens are both located in the second cavity and against the top of the second cavity, the intermediate fixing device 27 is provided with a first connecting ear 28 in front of the second cavity, and the intermediate fixing device 27 is provided with a second connecting ear 29 at the rear of the second cavity, the first connecting ear 28 and the second connecting ear 29 are both connected to the bracket 41 to fix the intermediate fixing device 27, so as to facilitate the smooth progress of the detection work. Among them, there are connecting parts between the first connecting ear 28 and the bracket 41, and between the second connecting ear 29 and the bracket 41, and the first connecting ear 28 and the second connecting ear 29 are both connected to the bracket 41 through the connecting part 20, so as to facilitate installation. It can be understood that the detection pool 26 can be an electrochemical reaction cell, a flow cell, etc.

[0039] The receiving component 3 receives and detects the light refracted from the lens. Figure 2 and Figure 3 In some embodiments, the receiving assembly 3 includes a second collimating lens 31, a first beam expanding lens 32, a second beam expanding lens 33, a cylindrical mirror 34 and a light intensity detection device 35 arranged in sequence along the light irradiation direction, the receiving through hole includes a first receiving through hole and a second receiving through hole connected to each other, a receiving tube 36 is arranged in the first receiving through hole, and the second collimating lens 31, the first beam expanding lens 32, the second beam expanding lens 33 and the cylindrical mirror 34 are all located in the receiving tube 36. The linearly polarized light refracted from the lens becomes parallel light after passing through the second collimating lens 31, and the parallel light is gathered after passing through the first beam expanding lens 32. After the parallel light is reduced, it passes through the second beam expanding lens 33 and the cylindrical mirror 34, and the width and length of the gathered parallel light can be adjusted. Among them, the setting of the length and width of the parallel light is determined by the effective detection range of the light intensity detection device 35. By setting the receiving tube 36, different components inside the receiving tube 36 can be easily replaced, so as to receive the light refracted by the lens, reduce the installation difficulty of the device, and facilitate maintenance and operation.

[0040] The light intensity detection device 35 can be a linear array CCD, an array CCD, a photocell, etc. Figure 2 and Figure 3 The light intensity detection device 35 is installed in the second receiving through hole, and the parallel light passing through the second beam expander lens 33 and the cylindrical mirror 34 can be incident on the light intensity detection device 35 for detection.

[0041] See also Figure 2In some embodiments, a fixing groove penetrating the top of the receiving fixture 6 is provided on the receiving fixture 6, the fixing groove is located between the receiving tube 36 and the light intensity detection device 35, and an attenuation sheet 37 is provided in the fixing groove, and the attenuation sheet 37 filters the light intensity to ensure that the light intensity can be detected by the light intensity detection device 35. A fixing seat 38 is provided on the top of the attenuation sheet 37, and the attenuation sheet 37 is connected to the receiving fixture 6 through the fixing seat 38. The setting of the fixing groove and the fixing seat 38 facilitates the installation and removal of the attenuation sheet 37 to meet different detection requirements.

[0042] In the description of this specification, the description with reference to the terms "example", "embodiment" or "some embodiments" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0043] Of course, the invention is not limited to the above-mentioned embodiments, and those skilled in the art may make equivalent modifications or substitutions without violating the spirit of the invention, and these equivalent modifications or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A surface plasmon resonance detection device, characterized in that: include A detection mechanism, comprising an incident component, an intermediate component and a receiving component, wherein the intermediate component is located between the incident component and the receiving component, the intermediate component comprises an optical coupling device, the incident component is used to generate linearly polarized light incident to the optical coupling device, and the receiving component is used to receive and detect the linearly polarized light refracted by the optical coupling device; The adjustment mechanism includes a first adjustment component and a second adjustment component, wherein the first adjustment component is used to change the posture of the incident component to adjust the incident angle of the linearly polarized light in the optical coupling device, and the second adjustment component is used to change the posture of the receiving component so that the receiving component can receive the linearly polarized light refracted by the optical coupling device. The adjustment mechanism also includes a bracket, and the intermediate component is installed on the bracket. The first adjustment component includes a first support seat, and the incident component is installed on the first support seat. The second adjustment component includes a second support seat, and the receiving component is installed on the second support seat. The first support seat and the second support seat are both hinged to the bracket. The optical coupling device includes a lens, and the intermediate component also includes a lens mounting seat, and the lens mounting seat is provided with a lens mounting groove for mounting the lens. The lens mounting seat is provided with openings on both sides of the lens mounting groove, and the openings are connected to the lens mounting groove. The lens mounting seat is provided with lens blocks at both ends of the lens mounting groove.

2. The surface plasmon resonance detection device according to claim 1, characterized in that: A base is provided at the bottom of the bracket, and an adjustment hole and a first locking hole are provided on the first support seat and the second support seat. Second locking holes are provided at both ends of the base. The first support seat and the second support seat are connected to the base through locking screws and adjusting screws. The locking screw passes through the first locking hole and extends into the second locking hole. The adjusting screw passes through the adjusting hole and rests against the base.

3. The surface plasmon resonance detection device according to claim 2, characterized in that: The base is provided with mounting grooves on both sides of the bracket, and an adjusting pad is provided in the mounting groove. The adjusting screw passes through the adjusting hole and abuts against the adjusting pad, and the second locking hole is located on the adjusting pad.

4. The surface plasmon resonance detection device according to claim 1, characterized in that: The first support seat is provided with an incident fixing device, and the incident fixing device is provided with an incident through hole for installing the incident component. The second support seat is provided with a receiving fixing device, and the receiving fixing device is provided with a receiving through hole for installing the receiving component.

5. The surface plasmon resonance detection device according to claim 4, characterized in that: The incident component includes a light source, a first aperture, a first collimating lens, a polarizer, a second aperture and a focusing lens which are sequentially arranged along the light irradiation direction; the incident through hole includes a first incident hole and a second incident hole which are connected to each other; the light source and the first aperture are both installed in the first incident hole; an incident tube is provided in the second incident hole; the first collimating lens, the polarizer, the second aperture and the focusing lens are all located in the incident tube.

6. The surface plasmon resonance detection device according to claim 4, characterized in that: The receiving component includes a second collimating lens, a first beam expanding lens, a second beam expanding lens, a cylindrical mirror and a light intensity detection device which are arranged in sequence along the light irradiation direction; the receiving through hole includes a first receiving through hole and a second receiving through hole which are connected to each other; a receiving tube is provided in the first receiving through hole; the second collimating lens, the first beam expanding lens, the second beam expanding lens and the cylindrical mirror are all located in the receiving tube; and the light intensity detection device is installed in the second receiving through hole.

7. The surface plasmon resonance detection device according to claim 6, characterized in that: The receiving fixture is provided with a fixing groove which passes through the top of the receiving fixture, the fixing groove is located between the receiving tube and the light intensity detection device, an attenuation plate is provided in the fixing groove, a fixing seat is provided on the top of the attenuation plate, and the attenuation plate is connected to the receiving fixture via the fixing seat.

8. The surface plasmon resonance detection device according to claim 1, characterized in that: The intermediate component also includes an intermediate fixing device, which has a first cavity and a second cavity that are connected to each other, the second cavity is located at the bottom of the first cavity, the first cavity is used to install the detection pool, the lens mounting seat and the lens are both located in the second cavity and against the top of the second cavity, the intermediate fixing device is provided with a first connecting ear in front of the second cavity, and the intermediate fixing device is provided with a second connecting ear at the rear of the second cavity, and the first connecting ear and the second connecting ear are both connected to the bracket.

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