Detoxification and propagation expanding method of sweet potatoes

Abstract

The invention relates to a detoxification and propagation expanding method of sweet potatoes. The detoxification and propagation expanding method mainly comprises the steps of explant collection and disinfection treatment, differentiation and induction culture, multiplication culture, rooting culture, seedling hardening and transplanting, field management and timely harvesting. By the adoption ofthe propagation expanding method, under the aseptic condition, through tissue culture and seedling hardening treatment, the transplanting survival rate of detoxification seedlings obtained after tissue culture can reach 100%, the serious problem of sweet potato virus diseases caused by propagation of sweet potato blocks or sweet potato vines in recent years is solved, the problems of variety quality deterioration, germplasm degeneration, yield reduction and the like are effectively prevented, and the germplasm purity is guaranteed; after detoxification, the germplasm is purified on the basis of keeping the variety characteristics of the original variety, the epidemic resistance of the variety is enhanced, and the quality and the yield are improved; and according to the method, the detoxification seedlings which are not limited by seasons and can be multiply and rapidly propagated in a high-coefficient mode can be obtained, industrialized seedling production and high-efficiency production can be achieved, and the detoxification seedlings can be widely applied to production.

Description

technical field

[0001] The invention belongs to the technical field of sweet potato cultivation in the agricultural field, and in particular relates to a virus-free and multiplication method for sweet potatoes. Background technique

[0002] Sweet potato is an annual or perennial vine of the genus Dioscorea in the family Dioscoreaceae, also known as sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, etc. Its growth characteristics are light-loving and warm-loving, more drought-tolerant, but not cold-resistant. The nutritional value of sweet potatoes is particularly high. It can not only provide a large amount of calories for the human body, but also provide the human body with protein, sugar, calcium and various trace elements such as phosphorus and iron. In addition, sweet potatoes also contain a large amount of linoleic acid and Nick Acid and a variety of vitamins, these nutrients can speed up the digestion of the stomach and intestines, an...

Examples

Embodiment 1

[0027] A method for virus-free and multiplication of sweet potatoes, the method for virus-free and multiplication mainly includes explant collection and disinfection treatment, differentiation and induction culture, multiplication culture, rooting culture, hardening and transplanting, field management and timely harvesting , including the following steps:

[0028] (1) Explant collection and disinfection treatment: choose healthy potato seedlings without diseases and insect pests as explants, cut off the stems and tendrils with stem tips, remove the visible leaves, and wash them directly with tap water for 1 to 1.5 hours. and filter dry with sterile gauze; then put it on the aseptic operating table, first wipe it once with sterile absorbent cotton soaked in 75% alcohol, after wiping, fully soak it in 75% alcohol for 30-40 seconds, take it out and use the weight Sterilize with mercuric chloride solution with a percentage of 0.1% for 10 to 15 minutes, and finally rinse with steri...

Embodiment 2

[0037] A method for virus-free and multiplication of sweet potatoes, the main steps are the same as in Example 1, and the difference is: one, in the field management process of the step (6), the topdressing is made of plant ash, ternary compound fertilizer, The compound fertilizer that urea and organic fertilizer are mixed forms, spreads 1300 kilograms of plant ash per mu, 40 kilograms of ternary compound fertilizers, 20 kilograms of urea and 1000 kilograms of organic fertilizers; The MS culture medium of mg / L kinetin KT, 0.08 mg / L NAA and 1.5mg / L BAP, described MS culture medium also contains the sucrose that mass concentration is 3.5%, the agar that mass concentration is 6g / L, regulates MS The pH of the medium is 5.8-6.2. The proliferation medium is selected to add MS medium with a mass concentration of 0.08mg / LNAA and 0.8mg / L BAP, and the MS medium also contains sucrose with a mass concentration of 3.5%, and agar with a mass concentration of 6g / L , adjust the pH of the MS ...

Embodiment 3

[0039] A method for detoxification and multiplication of sweet potatoes, the main steps are the same as in Example 1, and the difference is: one, wherein in the field management process of the step (6), the topdressing is made of plant ash, ternary compound fertilizer , urea and the compound fertilizer that organic fertilizer mixes to form, spread 1500 kilograms of plant ash by every mu, 45 kilograms of ternary compound fertilizers, 25 kilograms of urea and 1200 kilograms of organic fertilizers; The MS culture medium of 0.1 mg / L kinetin KT, 0.1 mg / L NAA and 2.0 mg / L BAP, described MS culture medium also comprises the sucrose that mass concentration is 3.5%, the agar that mass concentration is 6g / L, regulates The pH of the MS medium is 5.8-6.2. The proliferation medium is selected to add MS medium with a mass concentration of 0.1 mg / LNAA and 1.0 mg / L BAP, and the MS medium also contains sucrose with a mass concentration of 3.5%, and agar with a mass concentration of 6g / L , adj...

technical field

[0001] The invention belongs to the technical field of sweet potato cultivation in the agricultural field, and in particular relates to a virus-free and multiplication method for sweet potatoes. Background technique

[0002] Sweet potato is an annual or perennial vine of the genus Dioscorea in the family Dioscoreaceae, also known as sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, sweet potato, etc. Its growth characteristics are light-loving and warm-loving, more drought-tolerant, but not cold-resistant. The nutritional value of sweet potatoes is particularly high. It can not only provide a large amount of calories for the human body, but also provide the human body with protein, sugar, calcium and various trace elements such as phosphorus and iron. In addition, sweet potatoes also contain a large amount of linoleic acid and Nick Acid and a variety of vitamins, these nutrients can speed up the digestion of the stomach and intestines, an...

Examples

Embodiment 1

[0027] A method for virus-free and multiplication of sweet potatoes, the method for virus-free and multiplication mainly includes explant collection and disinfection treatment, differentiation and induction culture, multiplication culture, rooting culture, hardening and transplanting, field management and timely harvesting , including the following steps:

[0028] (1) Explant collection and disinfection treatment: choose healthy potato seedlings without diseases and insect pests as explants, cut off the stems and tendrils with stem tips, remove the visible leaves, and wash them directly with tap water for 1 to 1.5 hours. and filter dry with sterile gauze; then put it on the aseptic operating table, first wipe it once with sterile absorbent cotton soaked in 75% alcohol, after wiping, fully soak it in 75% alcohol for 30-40 seconds, take it out and use the weight Sterilize with mercuric chloride solution with a percentage of 0.1% for 10 to 15 minutes, and finally rinse with steri...

Embodiment 2

[0037] A method for virus-free and multiplication of sweet potatoes, the main steps are the same as in Example 1, and the difference is: one, in the field management process of the step (6), the topdressing is made of plant ash, ternary compound fertilizer, The compound fertilizer that urea and organic fertilizer are mixed forms, spreads 1300 kilograms of plant ash per mu, 40 kilograms of ternary compound fertilizers, 20 kilograms of urea and 1000 kilograms of organic fertilizers; The MS culture medium of mg / L kinetin KT, 0.08 mg / L NAA and 1.5mg / L BAP, described MS culture medium also contains the sucrose that mass concentration is 3.5%, the agar that mass concentration is 6g / L, regulates MS The pH of the medium is 5.8-6.2. The proliferation medium is selected to add MS medium with a mass concentration of 0.08mg / LNAA and 0.8mg / L BAP, and the MS medium also contains sucrose with a mass concentration of 3.5%, and agar with a mass concentration of 6g / L , adjust the pH of the MS ...

Embodiment 3

[0039] A method for detoxification and multiplication of sweet potatoes, the main steps are the same as in Example 1, and the difference is: one, wherein in the field management process of the step (6), the topdressing is made of plant ash, ternary compound fertilizer , urea and the compound fertilizer that organic fertilizer mixes to form, spread 1500 kilograms of plant ash by every mu, 45 kilograms of ternary compound fertilizers, 25 kilograms of urea and 1200 kilograms of organic fertilizers; The MS culture medium of 0.1 mg / L kinetin KT, 0.1 mg / L NAA and 2.0 mg / L BAP, described MS culture medium also comprises the sucrose that mass concentration is 3.5%, the agar that mass concentration is 6g / L, regulates The pH of the MS medium is 5.8-6.2. The proliferation medium is selected to add MS medium with a mass concentration of 0.1 mg / LNAA and 1.0 mg / L BAP, and the MS medium also contains sucrose with a mass concentration of 3.5%, and agar with a mass concentration of 6g / L , adj...