Cartridges and molecular diagnostic platforms

Abstract

The present application relates to the field of molecular detection technology, and in particular to a cartridge and a molecular diagnostic platform. The cartridge includes a cartridge body, a first pipetting valve, a test tube rack and a transfer unit; the cartridge body is formed with a plurality of first reagent compartments, the test tube rack is connected to the cartridge body and a test tube is installed in the test tube rack; the first pipetting valve is movably connected to the cartridge body, the first pipetting valve is formed with a first valve cavity, and as the first pipetting valve moves to different positions, the first valve cavity can be respectively connected to a plurality of first reagent compartments, so that reagent transfer can be performed between the first pipetting valve and the first reagent compartments connected thereto, and the first pipetting valve can transfer the reagent in the first valve cavity to the test tube through the transfer unit; therefore, when extracting sample nucleic acid, the transfer of reagents can be completed efficiently and fully, and the operation is simple; it is not easy to cause contamination to the sample during the whole process, so as to ensure the purity of the extract and the accuracy of the experimental results; at the same time, the cartridge has a simple structure and low equipment cost.

Description

Technical Field

[0001] The present invention relates to the technical field of molecular detection, and in particular to a card box and a molecular diagnosis platform. Background Art

[0002] For example, nucleic acid extraction is required in the process of molecular detection. Nucleic acid extraction generally requires steps such as lysis, binding, rinsing and elution. Reagents need to be transferred in each step. Manual transfer is mostly used in the prior art, that is, the raw materials or intermediates are manually transferred in the reaction vessel. Obviously, the manual transfer method is not only cumbersome, time-consuming and labor-intensive, but also very easy to cause contamination during the entire operation process, affecting the purity of the extract, and it is difficult to transfer the materials fully and efficiently, affecting the experimental results. Summary of the invention

[0003] The object of the present invention is to provide a cartridge and a molecular diagnostic platform to realize convenient and rapid transfer of reagents between chambers during nucleic acid extraction.

[0004] The present invention provides a cartridge, comprising a cartridge body, a first pipetting valve and a test tube rack; the cartridge body is formed with a plurality of first reagent chambers; the first pipetting valve is movably connected to the cartridge body, and the first pipetting valve is formed with a first valve cavity; the first pipetting valve can move so that the first valve cavity is respectively connected with the plurality of first reagent chambers; the test tube rack is used for placing a plurality of test tubes; the test tube rack is connected to the cartridge body, and the test tube rack is formed with a plurality of mounting holes, the plurality of mounting holes correspond one-to-one to the plurality of test tubes, and the tube mouths of the plurality of test tubes are inserted into the corresponding mounting holes; the cartridge also comprises a transfer portion, and the first pipetting valve can transfer the reagent in the first valve cavity to the test tubes through the transfer portion.

[0005] Further, the test tube is a capillary; the cartridge body is formed with a plurality of liquid inlet holes, which correspond one to one with the test tubes; the plurality of liquid inlet holes are each provided with a drainage needle, a drainage channel is formed inside the drainage needle, and one end of the drainage needle is connected with the liquid inlet hole; the test tube rack can be placed on the cartridge body, and the plurality of drainage needles can pass through the mounting hole and extend into the corresponding test tubes; the transfer portion can be connected with the liquid inlet hole.

[0006] Furthermore, it also includes a sealing cover plate; the sealing cover plate is detachably connected to the card box body; a plurality of sealing plugs are arranged on the plate surface of the sealing cover plate on the side away from the card box body, and the plurality of sealing plugs correspond to the plurality of test tubes one by one; the test tube rack can be snapped onto the sealing cover plate, and the sealing plugs can seal the test tube openings of the corresponding test tubes.

[0007] Further, the card box is formed with a first valve cabin; the first pipetting valve is movably connected in the first valve cabin; the card box body is formed with multiple first flow channels, the multiple first flow channels correspond one-to-one to the multiple first reagent cabins, and one end of the multiple first flow channels is connected to the corresponding first reagent cabin, and the other end of the multiple first flow channels is connected to the first valve cabin; the first pipetting valve is formed with a first pipetting port connected to the first valve cavity, and when the first pipetting valve moves, the first pipetting port can be connected to the multiple first flow channels respectively, thereby connecting the first valve cavity with the corresponding first reagent cabin.

[0008] Further, the transfer part includes a fifth flow channel; the card box body is formed with the fifth flow channel, the number of the fifth flow channels is multiple, and the multiple fifth flow channels correspond one-to-one to the multiple liquid inlet holes; one end of the multiple fifth flow channels is connected to the corresponding liquid inlet holes, and the other end of the multiple fifth flow channels is connected to the first valve compartment; the first pipetting valve can move so that the first pipetting port is connected to one end of the fifth flow channel.

[0009] Furthermore, the transfer part includes a second pipetting valve; the second pipetting valve is movably connected to the cartridge body, and the second pipetting valve forms a second valve cavity; the second pipetting valve can move so that the second valve cavity is respectively connected to the first valve cavity and the test tube.

[0010] Furthermore, the cartridge body is formed with a second valve compartment and a second flow channel, and the two ends of the second flow channel are respectively connected to the second valve compartment and the first valve compartment; the first pipette valve is movable so that the first pipette port is connected to one end of the second flow channel; the second pipette valve is movably connected in the second valve compartment, and the second pipette valve is also formed with a second pipette port connected to the second valve cavity; the second pipette valve is movable so that the second pipette port is connected to the other end of the second flow channel.

[0011] Furthermore, the cartridge body is formed with a second reagent chamber and a third flow channel; one end of the third flow channel is connected to the second reagent chamber; and the other end of the third flow channel is connected to the second pipetting port through the movement of the second pipetting valve.

[0012] Furthermore, the card box body is formed with multiple fourth flow channels, and the multiple fourth flow channels correspond one-to-one to the multiple liquid inlet holes, and the multiple liquid inlet holes are respectively connected with the second valve cabin through the corresponding fourth flow channels; the second pipetting valve can move so that the second pipetting port is respectively connected with the multiple fourth flow channels.

[0013] Further, the first transfer valve is rotatably connected in the first valve cabin; a first sealing gasket is provided on the side wall of the first transfer valve to seal and abut the side wall of the first transfer valve against the inner wall of the first valve cabin; a plurality of first through holes are provided on the side wall of the first valve cabin in contact with the first sealing gasket, and the plurality of first through holes correspond to and are connected with the second flow channel and the plurality of first flow channels one by one, and the plurality of first through holes are distributed at circumferential intervals; the first transfer valve can be rotatable so that the first transfer port is respectively connected with the plurality of first through holes.

[0014] Further, the second pipetting valve is rotatably connected in the second valve cabin; a second sealing gasket is provided on the side wall of the second pipetting valve to seal the side wall of the second pipetting valve against the inner wall of the second valve cabin; a plurality of second through holes are provided on the side wall of the second valve cabin in contact with the second sealing gasket, and the plurality of second through holes correspond to and are connected with the second flow channel, the third flow channel and the plurality of fourth flow channels one by one, and the plurality of second through holes are distributed at circumferential intervals; the second pipetting valve can be rotatable so that the second pipetting port is respectively connected with the plurality of second through holes.

[0015] Furthermore, a third through hole is formed on the bottom wall of each of the plurality of first reagent compartments, and the first reagent compartment is connected to the corresponding first flow channel through the third through hole.

[0016] Furthermore, a needle tip portion is formed on the bottom wall of some of the first reagent chambers among the multiple first reagent chambers, and the needle tip portion is arranged around the third through hole on the bottom wall of the first reagent chamber; the first reagent chamber formed with the needle tip portion is used to accommodate a reagent tube, and a thin film is provided on the bottom wall of the reagent tube.

[0017] Furthermore, a flange portion is formed at one end of the reagent tube located outside the first reagent chamber, and the flange portion can be overlapped on the card box body; the card box body is also provided with a pressure ring, one end of which is formed with a stud, and is threadedly connected to the card box body through the stud; the flange portion is located between the pressure ring and the card box body, and rotating the pressure ring can press the flange portion against the card box body.

[0018] Furthermore, the volume of the second valve chamber is smaller than the volume of the first valve chamber.

[0019] The present invention also provides a molecular diagnostic platform, comprising any of the above-mentioned cartridges.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The cartridge provided by the present invention comprises a cartridge body, a first pipetting valve and a test tube rack; the cartridge body is formed with a plurality of first reagent chambers; the first pipetting valve is movably connected to the cartridge body, the first pipetting valve is formed with a first valve cavity, and as the first pipetting valve moves to different positions, the first valve cavity can be respectively connected with the plurality of first reagent chambers, so that reagent transfer can be performed between the first pipetting valve and the first reagent chamber connected thereto, that is, the first pipetting valve can extract the reagent in the first reagent chamber connected thereto into the first valve cavity, or transport the reagent in the first valve cavity to the first reagent chamber connected thereto; the test tube rack is connected to the cartridge body, the test tube rack is formed with a mounting hole, a test tube is inserted in the mounting hole, and the tube mouth end of the test tube is connected to the mounting hole; the cartridge also comprises a transfer portion, through which the reagent in the first valve cavity can be transferred to the test tube, thereby completing the filling of the test tube. Therefore, when extracting nucleic acid from a sample, the card box of the present application can efficiently and fully complete the transfer of various reagents, and the operation is simple; and it is not easy to cause contamination to the sample during the entire process, ensuring the purity of the extract and the accuracy of the experimental results; at the same time, the card box has a simple structure and low equipment cost.

[0022] The present invention also provides a molecular diagnosis platform, comprising the cartridge, so the molecular diagnosis platform also has the beneficial effects of the cartridge. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 A schematic diagram of the structure of a card box provided by an embodiment of the present invention from a first viewing angle;

[0025] Figure 2 A schematic diagram of the split structure of the card box provided in an embodiment of the present invention;

[0026] Figure 3 A schematic diagram of the structure of a card box provided by an embodiment of the present invention from a second viewing angle;

[0027] Figure 4 for Figure 3 Sectional view of AA in the middle;

[0028] Figure 5 A cross-sectional schematic diagram of a test tube rack provided in an embodiment of the present invention;

[0029] Figure 6 A schematic structural diagram of a card box provided by an embodiment of the present invention from a third viewing angle;

[0030] Figure 7 A schematic structural diagram of a first liquid transfer valve provided in an embodiment of the present invention;

[0031] Figure 8 for Figure 3 Cross-sectional view of BB.

[0032] Reference numerals:

[0033] 1-cartridge body, 11-first valve compartment, 12-second valve compartment, 13-first reagent compartment, 13a-sample processing compartment, 13b-waste liquid compartment, 14-second reagent compartment, 15-first flow channel, 16-second flow channel, 17-third flow channel, 18-fourth flow channel, 19-needle tip, 2-first pipetting valve, 21-first valve cavity, 22-first pipetting port, 23-cylinder body, 24-piston rod, 25-piston head, 26-first sealing pad, 3-second pipetting valve, 4-test tube rack, 41-mounting hole, 42-test tube, 43-drainage needle, 44-liquid inlet hole, 5-reagent tube, 51-flanged edge, 6-pressure ring, 7-sealing cover plate, 71-sealing plug. DETAILED DESCRIPTION

[0034] The technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0035] The components of the embodiments of the present invention generally described and shown in the drawings herein may be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention.

[0036] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present invention.

[0037] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0038] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0039] Refer to the following Figures 1 to 8 The cartridge and molecular diagnostic platform according to the embodiments of the present application are described.

[0040] The present application provides a card box, such as Figures 1 to 3 As shown, the cartridge includes a cartridge body 1 and a first pipetting valve 2. In an embodiment, the cartridge body 1 is formed with a first valve compartment 11, a first reagent compartment 13 and a first flow channel 15 (for example, see Figure 6 ); the number of the first reagent chamber 13 and the first flow channel 15 are both multiple and one-to-one corresponding, and the multiple first reagent chambers 13 are connected to the first valve chamber 11 through the corresponding first flow channel 15.

[0041] In the embodiment, a first valve cavity 21 and a first pipetting port 22 connected to the first valve cavity 21 are formed in the first pipetting valve 2; the first pipetting valve 2 is movably connected to the first valve cabin 11, and the first pipetting valve 2 can move (for example, rotate) so that the first pipetting port 22 is connected to the corresponding first flow channel 15, and then the first valve cavity 21 is connected to the corresponding first reagent cabin 13.

[0042] In an embodiment, for example Figure 7 As shown, the first pipetting port 22 is formed at the bottom of the first pipetting valve 2. When the first valve cavity 21 is connected with the corresponding first reagent chamber 13 through the first pipetting port 22 and the corresponding first flow channel 15, the liquid in the first valve cavity 21 is discharged to the corresponding first reagent chamber 13, or the liquid in the corresponding first reagent chamber 13 is sucked into the first pipetting port 22 by the reciprocating motion of the piston head described below in the cylinder body described below. The detailed operation of the piston head will be described in detail below.

[0043] The card box provided in the present application includes a card box body 1 and a first pipetting valve 2; the card box body 1 is formed with a first valve compartment 11 and a plurality of first reagent compartments 13, and the plurality of first reagent compartments 13 are spaced apart and distributed around the first valve compartment 11; a plurality of first flow channels 15 are also formed at the bottom of the card box body 1, and the plurality of first flow channels 15 correspond one-to-one to the plurality of first reagent compartments 13, one end of each first flow channel 15 is connected to the first valve compartment 11, and the other end of each first flow channel 15 is connected to the corresponding first reagent compartment 13, thereby connecting the plurality of first reagent compartments 13 to the first valve compartment 11, so as to realize the transfer of liquid between the first valve compartment 11 and the corresponding first reagent compartment 13. Figure 1 and Figure 2 In the embodiment shown in FIG. 1 , the number of the first reagent chambers 13 is 9, but the present invention is not limited thereto and the number of the first reagent chambers may be adjusted according to the size of the cartridge and the test requirements.

[0044] As described above, the first pipette valve 2 is formed with a first valve cavity 21 and a first pipette port 22. The first valve cavity 21 has a certain volume, and the first pipette port 22 is connected to the first valve cavity 21. The reagent can enter or be discharged from the first valve cavity 21 of the first pipette valve 2 via the first pipette port 22; the first pipette valve 2 is movably connected to the first valve cabin 11, so that the first pipette valve 2 can move in the first valve cabin 11; as the first pipette valve 2 moves, the first pipette port 22 can be respectively connected to multiple first flow channels 15, and then connected to the first reagent cabin 13 corresponding to the first flow channels 15, so that the reagent can be transferred between the first pipette valve 2 and the first reagent cabin 13 connected thereto, that is, the first pipette valve 2 can extract the reagent in the first reagent cabin 13 connected thereto into the first valve cavity 21, or transport the reagent in the first valve cavity 21 to the first reagent cabin 13 connected thereto.

[0045] During specific use, one of the multiple first reagent chambers 13 is used as a sample processing chamber 13a, and the sample to be processed is placed in the sample processing chamber 13a; one is used as a waste liquid chamber 13b, for storing the waste liquid generated in each step of the reaction; the rest are used as reagent chambers, for storing various reagents used for sample processing, such as lysate, cleaning solution and eluent.

[0046] First, place the sample to be processed in the sample processing chamber 13a, and then close the sample processing chamber 13a. The sample processing chamber 13a will not be opened again during the whole sample processing process; then, according to the prescribed steps, the reagents in each reagent chamber are transferred to the sample processing chamber 13a in turn through the first pipetting valve 2 for reaction, and the waste liquid generated by each step of the reaction is transferred to the waste liquid chamber 13b through the first pipetting valve 2. For example, when it is necessary to add lysate to the sample processing chamber 13a, first move the first pipetting valve 2 so that the first valve chamber 21 of the first pipetting valve 2 is connected to the reagent chamber storing the lysate, and a certain amount of lysate is extracted into the first valve chamber 21 through the action of the piston assembly as described below; then continue to move the first pipetting valve 2 so that the first valve chamber 21 of the first pipetting valve 2 is connected to the sample processing chamber 13a, and the lysate in the first valve chamber 21 is transported to the sample processing chamber 13a through the action of the piston assembly as described below.

[0047] In this embodiment, if Figures 1 to 3 and Figure 6 As shown, the cartridge may further include a second pipetting valve 3; a second valve compartment 12 and a second flow channel 16 are formed on the cartridge body 1, one end of the second flow channel 16 is connected to the first valve compartment 11, and the other end of the second flow channel 16 is connected to the second valve compartment 12; the structure of the second pipetting valve 3 is the same as or similar to that of the first pipetting valve 2, and is provided with a second valve cavity and a second pipetting port connected to the second valve cavity; the second pipetting valve 3 is movably connected in the second valve compartment 12, and after the second pipetting valve 3 moves to a specific position, the second pipetting port can be connected to the second flow channel 16, and similarly, moving the first pipetting valve 2 to a specific position can also connect the first pipetting port 22 to the second flow channel 16; when the first pipetting port 22 and the second pipetting port are simultaneously connected to the second flow channel 16, the first valve cavity 21 is connected to the second valve cavity, thereby enabling the transfer of reagents between the first valve cavity 21 and the second valve cavity. The cartridge body 1 is also formed with a second reagent chamber 14 and a third flow channel 17, one end of the third flow channel 17 being connected to the second valve chamber 12, and the other end of the third flow channel 17 being connected to the second reagent chamber 14, so that when the second pipetting valve 3 located in the second reagent chamber 14 is moved to a specific position, the second pipetting port can be connected to the third flow channel 17, thereby connecting the second valve cavity to the second reagent chamber 14; therefore, the second valve cavity can be connected to the first valve cavity 21 or the second reagent chamber 14 by moving (for example, rotating) the second pipetting valve 3.

[0048] In specific use, the second reagent chamber 14 stores, for example, a PCR reaction system in the form of freeze-dried beads, including buffer, polymerase or reverse transcriptase, etc.; when the sample is extracted with nucleic acid in the sample processing chamber 13a, the first pipette valve 2 is first moved, specifically the first valve chamber 21 of the first pipette valve 2 is connected to the sample processing chamber 13a, and the nucleic acid solution after the nucleic acid extraction is extracted is extracted into the first valve chamber 21; then the first pipette valve 2 and the second pipette valve 3 are moved respectively, so that the first pipette valve 2 and the second pipette valve 3 are both connected to the second flow channel 16, and the nucleic acid solution in the first valve chamber 21 is transferred to the second valve chamber; then the second pipette valve 3 is continued to move, so that the second valve chamber is connected to the second reagent chamber 14, and the nucleic acid solution in the second valve chamber is transported to the second reagent chamber 14, so as to use the nucleic acid solution to re-dissolve the freeze-dried beads in the second reagent chamber 14. In the above operation process, the transfer of the nucleic acid solution between the first valve chamber 21 and the second valve chamber and between the second valve chamber and the second reagent chamber 14 is realized by the action of the piston assembly described below. In this embodiment, the dual pipette valve cartridge including the first pipette valve 2 and the second pipette valve 3 is used to enable the cartridge to perform more complex reagent transfers, thereby increasing the applicability and flexibility of the cartridge.

[0049] In this embodiment, if Figures 1 to 5 As shown, the cartridge further includes a test tube rack 4 connected to the cartridge body 1; the test tube rack 4 is provided with a plurality of mounting holes 41, each mounting hole 41 is provided with a test tube 42, the test tube 42 is a capillary tube, and the tube mouth of the test tube 42 is inserted into the corresponding mounting hole 41 with the tube mouth facing downward. The cartridge body 1 is provided with a plurality of liquid inlet holes 44, and the plurality of liquid inlet holes 44 correspond to the plurality of test tubes 42 one by one; the cartridge body 1 is also provided with a drainage needle 43 extending in the vertical direction, the drainage needle 43 has a drainage channel and an upper port and a lower port connected to the drainage channel formed inside, and the lower port of the drainage needle 43 is connected to the liquid inlet hole 44; when the test tube rack 4 is installed on the cartridge body 1, the drainage needle 43 can be inserted into the corresponding test tube 42, and the upper port of the drainage needle 43 extends to the upper end of the inside of the test tube 42.

[0050] The cartridge body 1 is formed with a plurality of fourth flow channels 18, which correspond one to one with the plurality of liquid inlet holes 44, and one end of the plurality of fourth flow channels 18 is connected to the second valve compartment 12, and the other end of the plurality of fourth flow channels 18 is connected through the plurality of liquid inlet holes 44; the movement of the second pipetting valve 3 enables the second pipetting port to be respectively connected with the plurality of fourth flow channels 18, so that the solution in the second valve cavity can enter the liquid inlet hole 44 at the lower end of the test tube rack 4 through the fourth flow channel 18, and then flow into the liquid flow channel inside the corresponding drainage needle 43, and finally be discharged from the port at the upper end of the drainage needle 43 and flow into the corresponding capillary; and by providing the drainage needle 43, the solution can gradually fill the capillary from the top of the capillary, so that the gas in the capillary can be discharged in the process of the capillary being gradually filled, thereby preventing the presence of bubbles in the solution when the solution in the capillary is tested, thereby preventing the accuracy of the test result from being affected.

[0051] In specific operations, there are multiple capillaries on the test tube rack, each capillary is placed with a set of primers and probes, and at least one set of primers and probes in the multiple capillaries is different from the primers and probes in other capillaries, so that multiple items can be tested on one sample. For example, if two items need to be tested on the sample, we only need to collect nucleic acid extracts from two capillaries, and different primers and probes are placed in the two capillaries.

[0052] Specifically, after the freeze-dried beads are reconstituted, the second pipette valve 3 moves to connect the second valve chamber with the second reagent chamber 14, and the reconstituted solution is drawn into the second valve chamber; then the second pipette valve 3 continues to move, so that the second pipette port is connected to the corresponding test tubes 42 through multiple fourth flow channels 18 in turn, and the solution in the second valve chamber is filled into multiple test tubes 42.

[0053] It should be noted that the volume of the eluate obtained after completing the nucleic acid extraction in the sample processing chamber 13a is small, and the volume used to reconstitute the freeze-dried beads and need to be filled into the capillary is even smaller; therefore, a second pipette valve 3 that is smaller than the first pipette valve 2 is set to transfer this part of the reagent, thereby improving the accuracy of the reagent transfer to a certain extent.

[0054] In summary, the card box of the present application can efficiently and fully complete the transfer of various reagents when performing nucleic acid extraction on samples, and the operation is simple; and it is not easy to cause contamination to the sample during the entire process, ensuring the purity of the extract and the accuracy of the experimental results; at the same time, the card box of the present application has a simple structure and can reduce the equipment cost to a certain extent.

[0055] In this embodiment, preferably, Figures 1 to 3As shown, the cartridge further includes a sealing cover plate 7, which is detachably connected to the cartridge body 1, and is provided with a plurality of sealing plugs 71, which are matched and correspond one to one with the plurality of test tubes 42 on the test tube rack 4; the test tube rack 4 can be removed from the cartridge body 1 and installed on the sealing cover plate 7, and the test tube openings of the test tubes 42 are sealed and plugged by the sealing plugs 71 on the sealing cover plate 7, so as to facilitate the subsequent transfer of reagents in the test tubes 42.

[0056] Preferably, a mounting groove is formed on the card box body 1, the sealing cover plate 7 can be placed in the mounting groove, and positioning grooves are opened on the bottom wall of the mounting groove at both ends along its length direction, and a limiting protrusion is formed on the side wall of the sealing cover plate 7 facing the bottom wall of the mounting groove. When the sealing cover plate 7 is installed in the mounting groove, the limiting protrusion can be inserted into the positioning groove, so that the sealing cover plate 7 and the card box body 1 are clamped together.

[0057] Preferably, the side wall of the sealing cover plate 7 facing away from the mounting groove, that is, the upper end surface of the sealing cover plate 7, is respectively formed with guide columns extending upward at both ends along its length direction, and the two ends of the test tube rack 4 in the length direction are formed with guide holes. When the test tube rack 4 is installed on the sealing cover plate 7, the guide holes are sleeved on the guide columns, so that the mounting hole 41 on the test tube rack 4 is directly opposite to the sealing plug 71 below.

[0058] Buckles are formed at both ends of the upper end surface of the sealing cover plate 7 along its length direction, and clamping holes are formed at both ends of the length direction of the test tube rack 4. When the test tube rack 4 is installed on the sealing cover plate 7, the buckles pass through the clamping holes to enable the sealing cover plate 7 to be clamped with the test tube rack 4, thereby ensuring that the sealing cover plate 7 can stably seal the test tube 42 during the transfer process.

[0059] In this embodiment, if Figure 7As shown, the first transfer valve 2 and the second transfer valve 3 have the same structure, so for the sake of clarity, the first transfer valve 2 is taken as an example to describe the corresponding structure of the first transfer valve 2 and the second transfer valve 3. In the embodiment, the first transfer valve 2 includes a cylinder body 23 and a piston assembly; a first valve cavity is formed in the cylinder body 23 of the first transfer valve 2, and a second valve cavity is formed in the cylinder body of the second transfer valve 3; the piston assembly includes a piston rod 24 and a piston head 25 connected to one end of the piston rod 24, the piston head 25 is located in the cylinder body 23, and the outer wall of the piston head 25 can be sealed against the inner wall of the cylinder body 23; by pushing and pulling the piston rod 24, the piston head 25 can be driven to reciprocate in the cylinder body 23 (in the embodiment shown in the figure, reciprocating in the vertical direction), and when pulled outward When the piston rod 24 is pushed inward, the volume of the first valve chamber 21 increases, and the first pipette valve 2 can extract the reagent into the enclosed space surrounded by the piston head 25 and the cylinder body 23; when the piston rod 24 is pushed inward, the volume of the first valve chamber 21 decreases, and the reagent in the cylinder body 23 can be discharged from the first pipette valve 2; therefore, it is only necessary to move the first pipette valve 2 or the second pipette valve 3 to a specific position to be connected with the corresponding first flow channel 15, second flow channel 16, third flow channel 17 or fourth flow channel 18, and the extraction and delivery of the reagent can be completed conveniently and quickly by pushing and pulling the piston rod 24.

[0060] In this embodiment, if Figure 1 and Figure 7 As shown, the first pipetting valve 2 is rotatably connected in the first valve cabin 11, and a plurality of first through holes are opened on the side wall of the first valve cabin 11, and the plurality of first through holes are distributed in a circumferential interval; one of the plurality of first through holes corresponds to and is connected with the second flow channel 16, and the remaining first through holes in the plurality of first through holes correspond to and are connected with the plurality of first flow channels 15 one by one; the first pipetting port 22 is located on the side wall of the cylinder body 23 of the first pipetting valve 2, and when the first pipetting valve 2 is rotated, the first pipetting port 22 can be respectively connected with the corresponding first through holes in the plurality of first through holes, so that the first valve cavity 21 is connected with the second valve cavity and each of the plurality of first reagent cabins 13.

[0061] In addition, as described above, a plurality of first through holes are distributed in the side wall of the first valve compartment 11 at intervals in the circumferential direction, so that the first transfer port 22 can be connected to the corresponding first flow channel 15 by rotating the first transfer valve 2. However, it is not limited to this, as long as the first transfer port can be connected to the corresponding first flow channel, the first transfer valve can also be moved in other ways. For example, as a variation, a plurality of first through holes are distributed on the side wall of the first valve compartment along the height direction. In this case, the first transfer port is connected to the corresponding first flow channel by moving the first transfer valve in the vertical direction. However, in this case, the first flow channel cannot be formed side by side at the bottom of the cartridge body as in the present application, but needs to be formed obliquely inside the cartridge body, that is, one end of the plurality of first flow channels is respectively connected to the first through holes at different height positions, and the other end of the plurality of first flow channels is respectively connected to the third through holes described below at the bottom of the first reagent compartment.

[0062] Preferably, in the embodiment, the side wall of the first pipetting valve 2 may be provided with a first sealing gasket 26 (for example, see Figure 7 ), the first sealing gasket 26 surrounds and wraps around the outer side of the cylinder body 23 of the first pipette valve 2, and when the first pipette valve 2 is rotated and connected to the first valve cabin 11, the first sealing gasket 26 is clamped between the outer wall of the cylinder body 23 of the first pipette valve 2 and the inner wall of the first valve cabin 11; multiple first through holes are located on the side wall of the first valve cabin 11 that fits the first sealing gasket 26, and the first pipette port 22 passes through the first sealing gasket 26; thus, when the first pipette valve 2 is rotated so that the first pipette port 22 is not connected to any first through hole, the first sealing gasket 26 can seal and block all the first through holes, cutting off the connection between the first valve cabin 11 and each of the multiple first reagent cabins 13 and the second valve chamber; when the first pipette valve 2 is rotated so that the first pipette port 22 is connected to one of the multiple first through holes, the first sealing gasket 26 can seal and block the remaining first through holes, so that the first valve chamber 21 is only connected to the first flow channel 15 or the second flow channel 16 corresponding to the connected first through hole.

[0063] In this embodiment, if Figure 1 and Figure 7 As shown, the second pipetting valve 3 is rotatably connected in the second valve cabin 12, and a plurality of second through holes are opened on the side wall of the second valve cabin 12, and the plurality of second through holes are distributed in a circumferential interval; two of the plurality of second through holes respectively correspond to and are connected with the second flow channel 16 and the third flow channel 17, and the remaining second through holes respectively correspond to and are connected with the plurality of fourth flow channels 18; the second pipetting port is located on the side wall of the cylinder body of the second pipetting valve 3, and when the second pipetting valve 3 is rotated, the second pipetting port can be connected with the plurality of second through holes respectively, so that the second valve cavity is respectively connected with the first valve cavity 21, the second reagent cabin 14 or the plurality of test tubes 42.

[0064] In addition, as described above, the plurality of second through holes are spaced apart in the circumferential direction on the side wall of the second valve chamber 12, so that the second transfer port can be connected to the corresponding second flow channel 16, third flow channel 17 or fourth flow channel 18 by the rotation of the second transfer valve 3. However, it is not limited thereto, as long as the second transfer port can be connected to the corresponding second flow channel 16, third flow channel 17 or fourth flow channel 18, the second transfer valve 3 can also be moved in other ways, for example, similar to the modification examples of the plurality of first through holes and the first transfer valve 2, which will not be described in detail here.

[0065] Similarly, preferably, in the embodiment, the side wall of the second transfer valve 3 may also be provided with a second sealing gasket, which surrounds and wraps around the outer side of the cylinder of the second transfer valve 3. When the second transfer valve 3 is rotated and connected to the second valve cabin 12, the second sealing gasket is clamped between the inner wall of the second valve cabin 12 and the outer wall of the cylinder of the second transfer valve 3, and the second transfer port passes through the second sealing gasket, and the second through hole is located on the side wall of the second valve cabin 12 that fits the second sealing gasket; thus, when the second transfer valve 3 is rotated to make the second transfer valve 3 When the pipette port is not connected to any second through hole, the second sealing gasket can seal all the second through holes, cutting off the connection between the second flow channel 16, the third flow channel 17 and multiple fourth flow channels 18 and the second valve cabin 12; when the second pipette valve 3 is rotated to connect the second pipette port to one of the second through holes, the second sealing gasket can seal the remaining second through holes, only connecting the second pipette port to the corresponding second flow channel 16, the third flow channel 17 or the fourth flow channel 18 through the second through hole.

[0066] In this embodiment, if Figures 1 to 3 and Figure 6 As shown, a third through hole is formed on the bottom wall of each first reagent compartment 13, and the first reagent compartment 13 is connected with the first valve compartment 11 through the corresponding first flow channel 15, one end of the first flow channel 15 is connected with the first through hole opened on the first valve compartment 11, and the other end of the first flow channel 15 is connected with the third through hole opened on the corresponding first reagent compartment 13, thereby connecting the first reagent compartment 13 with the corresponding first through hole on the first valve compartment 11; when the first pipetting port 22 is rotated until the first pipetting port 22 is connected to a first through hole, the first valve cavity 21 can be connected with the first reagent compartment 13 corresponding to the first through hole, thereby realizing the reagent transfer between the first pipetting valve 2 and the first reagent compartment 13.

[0067] In this embodiment, if Figure 1 and Figure 8As shown, when nucleic acid is extracted from the sample in the sample processing chamber 13a, special reagents such as reaction solution with high ion concentration and volatile alcohol cleaning solution are used. In order to prevent these special reagents from contacting the cylinder 23 of the first pipette valve 2 and the first reagent chamber 13 storing the reagents for a long time to cause crystallization or corrosion, these reagents are not directly stored in the corresponding first reagent chamber 13, but are pre-stored in the reagent tube 5. When these reagents are needed, the reagent tube 5 is placed in the corresponding first reagent chamber 13.

[0068] The bottom wall of the first reagent chamber 13 for placing these special reagents is formed with a needle tip 19, and the needle tip 19 is arranged around the third through hole at the bottom of the first reagent chamber 13, and a thin film is arranged at the bottom of the reagent tube 5 for storing special reagents. When the reagent tube 5 is placed in the corresponding first reagent chamber 13, the needle tip 19 can pierce the thin film at the bottom of the reagent tube 5, thereby connecting the reagent tube 5 with the third through hole, and the liquid can enter the corresponding first flow channel 15.

[0069] Specifically, multiple first reagent chambers 13 for placing special reagents can be arranged side by side, and the corresponding multiple reagent tubes 5 can be designed to be connected in parallel, so that the multiple reagent tubes 5 with the connected tube design can be placed together in the corresponding first reagent chambers 13.

[0070] In this embodiment, if Figure 1 and Figure 8 As shown, a flange portion 51 is formed at the upper end of the reagent tube 5. When the reagent tube 5 is placed in the first reagent compartment 13, the flange portion 51 is located outside the first reagent compartment 13, and the flange portion 51 can overlap the card box body 1 outside the first reagent compartment 13; the card box body 1 is provided with a pressure ring 6, one end of the pressure ring 6 is formed with a stud, and is threadedly connected to the card box body 1 through the stud, and rotating the pressure ring 6 can make the pressure ring 6 move in a direction close to or away from the card box body 1; the flange portion 51 of the reagent tube 5 is located between the card box body 1 and the pressure ring 6. When the pressure ring 6 is rotated to move the pressure ring 6 in a direction close to the card box body 1, the flange portion 51 can be pressed between the card box body 1 and the pressure ring 6, thereby fixing the reagent tube 5.

[0071] It should be noted that when multiple reagent tubes 5 adopt a connecting tube design and the corresponding first reagent chambers 13 are distributed side by side, only two pressure rings 6 need to be set on the card box body 1. The two pressure rings 6 are respectively set at the two ends of the reagent tube 5 with a connecting tube design to fix the reagent tube 5.

[0072] It should be noted that, in the above-mentioned embodiment, the filling of the capillary is completed by the cooperation of the first pipetting valve 2 and the second pipetting valve 3 , however, it is not limited thereto.

[0073] For example, in an embodiment of the present application, although not shown, the filling of the capillary can also be completed by only using the first pipette valve and omitting the second pipette valve; specifically, the cartridge body is formed with a plurality of fifth flow channels, and the plurality of fifth flow channels correspond one-to-one to a plurality of liquid inlet holes on the test tube rack; one end of the plurality of fifth flow channels is connected to the corresponding liquid inlet holes, and the other ends of the plurality of fifth flow channels are respectively connected to the first valve compartment, and when the first pipette valve moves, the first pipette port can be connected to one end of the fifth flow channels, thereby connecting the first valve cavity to the plurality of liquid inlet holes and the corresponding drainage needles, respectively, and then the solution after the nucleic acid extraction can be filled into the capillary through the first pipette valve.

[0074] It should be noted that the connection between the multiple fifth flow channels and the first valve cabin can be that one end of the multiple fifth flow channels are directly connected to the first valve cabin respectively, or one end of the multiple fifth flow channels are first converged into one flow channel and then connected to the first valve cabin.

[0075] The present application also provides a molecular diagnostic platform, comprising the cartridge of any of the above embodiments.

[0076] In this embodiment, the molecular diagnostic platform includes a cartridge, and thus the molecular diagnostic platform has all the beneficial effects of the cartridge, which will not be described in detail herein.

[0077] In addition, it should be noted that in the above-mentioned embodiment, the cartridge is used to realize convenient and quick transfer of reagents between various chambers during nucleic acid extraction. However, the use of the cartridge is not limited to this. According to the cartridge of the embodiment of the present application, the first valve compartment and the first reagent compartment are effectively connected by the operation of the first pipette valve, so as to quickly transfer the detection reagent. Obviously, this cartridge can also be applied to reagent detection in other fields, such as solution configuration, solution test detection, etc.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A card box, characterized in that: It includes a cartridge body, a first pipetting valve and a test tube rack; The cartridge body is formed with a plurality of first reagent compartments; The first liquid transfer valve is movably connected to the cartridge body, and the first liquid transfer valve is formed with a first valve cavity; The first liquid transfer valve is movable so that the first valve chamber is connected to the plurality of first reagent chambers respectively; The test tube rack is used to place multiple test tubes; the test tube rack is connected to the cartridge body, and the test tube rack forms multiple mounting holes, the multiple mounting holes correspond to the multiple test tubes one by one, and the tube ends of the multiple test tubes are inserted into the corresponding mounting holes; The cartridge further comprises a transfer portion, and the first pipetting valve can transfer the reagent in the first valve chamber to the test tube through the transfer portion; The cartridge body is formed with a plurality of liquid inlet holes, and the plurality of liquid inlet holes correspond one to one to the plurality of test tubes; The cartridge is formed with a first valve compartment; The first liquid transfer valve is movably connected in the first valve compartment; The cartridge body is formed with a plurality of first flow channels, the plurality of first flow channels correspond to the plurality of first reagent compartments one by one, one end of the plurality of first flow channels is connected to the corresponding first reagent compartment, and the other end of the plurality of first flow channels is connected to the first valve compartment; The first transfer valve is formed with a first transfer port connected to the first valve cavity. When the first transfer valve moves, the first transfer port can be connected to the plurality of first flow channels respectively, thereby connecting the first valve cavity to the corresponding first reagent chamber. The first pipetting port is formed at the bottom of the first pipetting valve.

2. The card box according to claim 1, characterized in that: The test tube is a capillary tube; A drainage needle is disposed on each of the plurality of liquid inlet holes, a drainage channel is formed inside the drainage needle, and one end of the drainage needle is connected to the liquid inlet hole; The test tube rack can be placed on the cartridge body, and the plurality of drainage needles can pass through the mounting holes and extend into the corresponding test tubes; The transfer portion can be communicated with the liquid inlet hole.

3. The card box according to claim 2, characterized in that: Also included is a sealing cover plate; The sealing cover plate is detachably connected to the card box body; A plurality of sealing plugs are arranged on a plate surface of the sealing cover plate at a side away from the cartridge body, and the plurality of sealing plugs correspond to the plurality of test tubes one by one; The test tube rack can be snap-connected to the sealing cover plate, and the sealing plug can seal and block the test tube opening of the corresponding test tube.

4. The card box according to claim 1, characterized in that: The transfer portion includes a fifth flow channel; The cartridge body is formed with the fifth flow channel, the number of the fifth flow channels is multiple, and the multiple fifth flow channels correspond to the multiple liquid inlet holes one by one; One end of each of the fifth flow channels is connected to the corresponding liquid inlet hole, and the other end of each of the fifth flow channels is connected to the first valve compartment; The first pipetting valve is movable so that the first pipetting port is connected to one end of the fifth flow channel.

5. The card box according to claim 1, characterized in that: The transfer unit includes a second pipetting valve; The second liquid transfer valve is movably connected to the cartridge body, and the second liquid transfer valve is formed with a second valve cavity; The second pipetting valve can move so that the second valve chamber is connected with the first valve chamber and the test tube respectively.

6. The card box according to claim 5, characterized in that: The cartridge body is formed with a second valve compartment and a second flow channel, and two ends of the second flow channel are respectively connected to the second valve compartment and the first valve compartment; The first pipetting valve is movable so that the first pipetting port is connected to one end of the second flow channel; The second transfer valve is movably connected in the second valve chamber, and the second transfer valve is further formed with a second transfer port communicated with the second valve chamber; The second pipetting valve can move so that the second pipetting port is connected to the other end of the second flow channel.

7. The card box according to claim 6, characterized in that: The cartridge body is formed with a second reagent chamber and a third flow channel; One end of the third flow channel is connected to the second reagent chamber; The other end of the third flow channel is connected to the second pipetting port through the movement of the second pipetting valve.

8. The card box according to claim 7, characterized in that: The cartridge body is formed with a plurality of fourth flow channels, the plurality of fourth flow channels correspond to the plurality of liquid inlet holes one by one, and the plurality of liquid inlet holes are respectively connected to the second valve compartment through the corresponding fourth flow channels; The second pipetting valve is movable so that the second pipetting port is communicated with the plurality of fourth flow channels respectively.

9. The card box according to claim 6, characterized in that: The first transfer valve is rotatably connected to the first valve chamber; A first sealing gasket is disposed on the side wall of the first transfer valve so that the side wall of the first transfer valve is sealed against the inner wall of the first valve chamber; A plurality of first through holes are formed on the side wall of the first valve compartment that is in contact with the first sealing gasket. The plurality of first through holes correspond to and are connected with the second flow channels and the plurality of first flow channels one by one, and the plurality of first through holes are distributed at intervals on the circumference. The first pipetting valve can rotate so that the first pipetting port is connected to the plurality of first through holes respectively.

10. The card box according to claim 8, characterized in that: The second transfer valve is rotatably connected in the second valve chamber; A second sealing gasket is disposed on the side wall of the second transfer valve so that the side wall of the second transfer valve is sealed against the inner wall of the second valve chamber; A plurality of second through holes are formed on the side wall of the second valve compartment that is in contact with the second sealing gasket. The plurality of second through holes correspond to and are connected with the second flow channel, the third flow channel and the plurality of fourth flow channels one by one, and the plurality of second through holes are distributed at intervals on the circumference. The second pipetting valve can rotate so that the second pipetting port is connected to the plurality of second through holes respectively.

11. The card box according to claim 4 or 5, characterized in that: The bottom walls of the plurality of first reagent compartments are each formed with a third through hole, and the first reagent compartment is connected with the corresponding first flow channel through the third through hole; The bottom walls of some of the first reagent compartments among the plurality of first reagent compartments are formed with needle tips, and the needle tips are arranged around the third through hole of the bottom wall of the first reagent compartment; The first reagent chamber formed with a needle tip is used to accommodate a reagent tube, and a film is provided on the bottom wall of the reagent tube.

12. The card box according to claim 11, characterized in that: A flange portion is formed at one end of the reagent tube located outside the first reagent chamber, and the flange portion can overlap the cartridge body; The card box body is also provided with a pressure ring, one end of which is formed with a stud, and is threadedly connected to the card box body through the stud; The flange portion is located between the pressure ring and the card box body, and rotating the pressure ring can press the flange portion onto the card box body.

13. The card box according to claim 5, characterized in that: The volume of the second valve chamber is smaller than the volume of the first valve chamber.

14. A molecular diagnostic platform, characterized in that: A card box comprising any one of claims 1-13.

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