A kind of simulated liquid of women's menstrual blood

Abstract

The present invention belongs to the technical field of blood simulation fluid, and particularly relates to a women's menstrual blood simulation fluid. The women's menstrual blood simulation fluid is made of the following raw materials: tertiary water, inorganic sodium salt, acid-base regulator, thickener, simulation modifier, simulation regulator, preservative and red pigment. The formula of the women's menstrual blood simulation fluid of the present invention is scientific and reasonable. By adding the simulation modifier and cooperating with other components in the simulation fluid, a product that is better than the existing standard test fluid is obtained. Compared with the existing standard fluid, it is closer to the physical properties of human blood. Compared with human and animal blood materials, it has the advantages of not easy to agglomerate, easy to preserve, and cheaper. It has a good guiding significance in the determination of the absorption and penetration properties of sanitary napkins and high-absorbent resins.

Description

[0001] This application is a divisional application of the invention application with application number 201710134444.8, application date March 8, 2017, and invention name "A women's menstrual blood simulation fluid and its preparation method". Technical Field

[0002] The invention belongs to the technical field of blood simulation liquid, and in particular relates to a women's menstrual blood simulation liquid. Background Art

[0003] In recent years, high molecular absorbent resins, the raw materials of sanitary products, have been developed through technological research and development to produce highly absorbent resin materials for sanitary napkins. In the past, the penetration and absorption properties of sanitary napkins and absorbent materials for sanitary napkins were generally tested with physiological saline and distilled water, but the physical properties of real women's menstrual blood and distilled water and physiological saline are quite different. With the continuous improvement of the detection method, the use of physiological saline and distilled water alone can no longer meet the needs of penetration and absorption performance testing, and cannot reflect the blood absorption performance of absorbent materials used in sanitary napkins and sanitary products. Later, people bought real human blood or used animal blood for testing, but real human blood and animal blood are not easy to obtain. Not only are they expensive, but they are also easy to clump and difficult to preserve.

[0004] At present, a blood-simulating liquid, i.e., a standard liquid formula, is used to test high-absorbent resins for women's sanitary products and sanitary napkins. It has been included in the national standard for "high-absorbent resins for sanitary napkins". However, research has shown that the pH value of this standard liquid is about 4 values ​​higher than the pH value of real women's menstrual blood, and its viscosity is about 7 values ​​greater than real blood. This results in the inability to approach the numerical value of the blood test product when testing the product. Therefore, the test results obtained using this solution cannot well reflect the characteristics of sanitary products in absorbing blood. The inaccurate test results also lead to unstable absorption performance of sanitary napkins and high-absorbent resin products, which has a certain impact on the upstream product material production and the quality of downstream products. Summary of the invention

[0005] The present invention is realized by the following technical scheme: a women's menstrual blood simulation liquid is made of the following raw materials in parts by weight:

[0006] 820-950 parts of tertiary water, 40-50 parts of inorganic sodium salt, 9-12 parts of acid-base regulator, 1-3 parts of thickener, 75-90 parts of simulated modifier, 0.05-0.3 parts of simulated regulator, 0.5-1.5 parts of preservative, 2-3 parts of red pigment.

[0007] The women's menstrual blood simulation liquid is prepared from the following raw materials in parts by weight:

[0008] 859.35 parts of tertiary water, 45 parts of inorganic sodium salt, 10 parts of acid-base regulator, 2 parts of thickener, 80 parts of simulated modifier, 0.15 parts of simulated regulator, 1 part of preservative, and 2.5 parts of red pigment.

[0009] The acid-base regulator is two or more of citric acid, phosphoric acid, sodium hydroxide, potassium carbonate, sodium carbonate, sodium citrate, and potassium dihydrogen phosphate.

[0010] The thickener is one or more of glycerin, sodium carboxymethyl cellulose, gelatin, polyvinyl alcohol, and gum arabic.

[0011] The simulated modifier is protein powder, soybean milk powder or milk powder.

[0012] The simulated regulator is one or more of fatty acid sanguisorbanol, lauric acid, sodium lauryl sulfate, sodium dodecylbenzene sulfonate, and glyceryl monostearate.

[0013] The preservative is lactic acid or sodium benzoate.

[0014] The inorganic sodium salt is sodium sulfate or sodium chloride.

[0015] The method for preparing the simulated menstrual blood liquid comprises the following steps:

[0016] a. Take the above-mentioned inorganic sodium salt, acid-base regulator, thickener, simulated regulator and preservative in parts by weight, pour them into a beaker, and stir to mix evenly;

[0017] b. Take the above-mentioned third-grade water in parts by weight and slowly pour it into the above-mentioned beaker, stirring with a glass rod while pouring; then put the beaker on a magnetic stirrer and stir for 15 minutes to obtain stirring liquid A;

[0018] c. Pour 200-300 mL of stirring liquid A in batches, place on a stirrer, stir for 4-8 minutes, and mix the liquid after each stirring to obtain stirring liquid B;

[0019] d. Pour 200-300 mL of stirring liquid B in batches, place on a stirrer, stir for 4-8 minutes, and mix the liquid after each stirring to obtain stirring liquid C;

[0020] e. Pour 200-300 mL of stirring liquid C in batches, place on a stirrer, stir for 4-8 minutes, and mix the liquid after each stirring to obtain stirring liquid D;

[0021] f. Filter the stirred liquid D through a 300-mesh filter cloth to obtain a filtrate;

[0022] g. Add the above-mentioned simulated modifier in parts by weight to the filtrate obtained in step f, stir until the simulated modifier is completely dissolved, put it on a magnetic stirrer, and stir it thoroughly for 15 minutes to obtain stirring solution E;

[0023] h. Add the above-mentioned red pigment in parts by weight to the stirred liquid E, stir evenly, and let it stand for not less than 36 hours.

[0024] The stirring speed of the magnetic stirrer is 600-900 r / min; the stirring speed of the mixer is 9000-15000 r / min.

[0025] Beneficial effects of the present invention:

[0026] 1. The formula of the simulated menstrual blood liquid of the present invention is scientific and reasonable. By adding a simulated modifier, it cooperates with other ingredients in the simulated liquid, such as inorganic sodium salt, acid-base regulator, thickener, simulated regulator, red pigment, etc., and after sufficient and multiple stirring, a product that is better than the existing standard test liquid is obtained. It has a good guiding significance in the determination of the absorption and penetration properties of sanitary napkins and superabsorbent resins. Manufacturers can use this test result to upgrade and improve upstream and downstream products to ensure the high quality and stable quality of the products.

[0027] 2. The preparation method of the women's menstrual blood simulation fluid of the present invention is simple and has strong operability. The prepared women's menstrual blood simulation fluid has a pH value of 7.20±0.5 at room temperature, a viscosity of 7.0±0.8mPa·s, a density of 1.06±0.05g / cm2, and a surface tension of 40±4mN / m. Compared with the existing standard fluid, it is closer to the physical properties of human blood. Compared with human and animal blood materials, it has the advantages of not easy to agglomerate, easy to preserve, and cheaper, and has good practical value and economic benefits. DETAILED DESCRIPTION

[0028] The present invention will be further described below in conjunction with embodiments, but the present invention is not limited thereto.

[0029] Embodiment 1:

[0030] The women's menstrual blood simulation fluid is made of the following raw materials by weight:

[0031] 859.35g of tertiary water, 45g of sodium chloride, 3g of sodium carbonate, 7g of potassium dihydrogen phosphate, 2g of glycerol, 80g of protein powder, 0.15g of sodium dodecylbenzene sulfonate, 1g of sodium benzoate, and 2.5g of red pigment.

[0032] The method for preparing the simulated menstrual blood liquid comprises the following steps:

[0033] a. Take the above weight of sodium chloride, sodium carbonate, potassium dihydrogen phosphate, glycerol, sodium dodecylbenzene sulfonate, and sodium benzoate, pour them into a beaker, and stir and mix them evenly with a glass rod;

[0034] b. Take the above weight of grade tertiary water and slowly pour it into the above beaker, stirring with a glass rod while pouring; then put the beaker on a magnetic stirrer and stir at a stirring speed of 800 r / min for 15 minutes to obtain solution A;

[0035] c. Pour 250 mL of stirring liquid A in batches, place them on a small stirrer, stir them at a stirring speed of 10000 r / min for 5 min, mix the liquid after each stirring, and obtain stirring liquid B;

[0036] d. Pour 250 mL of stirring liquid B in batches, place them on a small stirrer, stir at a stirring speed of 10000 r / min for 5 min, mix the liquid after each stirring, and obtain stirring liquid C;

[0037] e. Pour 250 mL of stirring liquid C in batches, place them on a small stirrer, stir them at a stirring speed of 10000 r / min for 5 min, mix the liquid after each stirring, and obtain stirring liquid D;

[0038] f. Filter the stirred liquid D through a 300-mesh filter cloth to obtain a filtrate;

[0039] g. Add the above-mentioned protein powder in parts by weight to the filtrate obtained in step f, stir until the protein powder is completely dissolved, put it on a magnetic stirrer, use a stirring speed of 800 r / min, and stir it thoroughly for 15 min to obtain stirring solution E;

[0040] h. Add the above-mentioned red pigment in parts by weight to the stirred liquid E, stir evenly, and let it stand for 38 hours.

[0041] Embodiment 2:

[0042] The women's menstrual blood simulation fluid is made of the following raw materials by weight:

[0043] 820g of tertiary water, 40g of sodium sulfate, 2g of sodium carbonate, 7g of potassium dihydrogen phosphate, 1g of glycerol, 75g of milk powder, 0.05g of sodium dodecylbenzene sulfonate, 0.5g of lactic acid, and 2g of red pigment.

[0044] The method for preparing the simulated menstrual blood liquid comprises the following steps:

[0045] a. Take the above weight of sodium sulfate, sodium carbonate, potassium dihydrogen phosphate, glycerol, sodium dodecylbenzene sulfonate and lactic acid, pour them into a beaker, and stir and mix them evenly with a glass rod;

[0046] b. Take the above weight of grade tertiary water and slowly pour it into the above beaker, stirring with a glass rod while pouring; then put the beaker on a magnetic stirrer, stir at a stirring speed of 600r / min for 15min to obtain solution A;

[0047] c. Pour 200 mL of stirring liquid A in batches, place them on a small stirrer, stir at a stirring speed of 9000 r / min for 4 min, mix the liquid after each stirring, and obtain stirring liquid B;

[0048] d. Pour 200 mL of stirring liquid B each time, place them on a small stirrer, stir at a stirring speed of 9000 r / min for 4 min, mix the liquid after each stirring, and obtain stirring liquid C;

[0049] e. Pour 200 mL of stirring liquid C in batches, place them on a small stirrer, stir at a stirring speed of 9000 r / min for 4 min, mix the liquid after each stirring, and obtain stirring liquid D;

[0050] f. Filter the stirred liquid D through a 300-mesh filter cloth to obtain a filtrate;

[0051] g. Add the above-mentioned soybean milk powder in parts by weight to the filtrate obtained in step f, stir until the soybean milk powder is completely dissolved, put it on a magnetic stirrer, stir it at a stirring speed of 600 r / min, and stir it for 15 minutes to obtain stirring liquid E;

[0052] h. Add the above-mentioned red pigment in parts by weight to the stirred liquid E, stir evenly, and let it stand for 40 hours.

[0053] Embodiment 3:

[0054] The women's menstrual blood simulation fluid is made of the following raw materials by weight:

[0055] 840.2g of tertiary water, 50g of sodium chloride, 5g of sodium carbonate, 7g of potassium dihydrogen phosphate, 3g of glycerol, 90g of soy milk powder, 0.3g of sodium dodecylbenzene sulfonate, 1.5g of sodium benzoate, and 3g of red pigment.

[0056] The method for preparing the simulated menstrual blood liquid comprises the following steps:

[0057] a. Take the above weight of sodium chloride, sodium carbonate, potassium dihydrogen phosphate, glycerol, sodium dodecylbenzene sulfonate, and sodium benzoate, pour them into a beaker, and stir and mix them evenly with a glass rod;

[0058] b. Take the above weight of grade tertiary water and slowly pour it into the above beaker, stirring with a glass rod while pouring; then put the beaker on a magnetic stirrer, stir at a stirring speed of 900 r / min for 15 minutes to obtain solution A;

[0059] c. Pour 300 mL of stirring liquid A in batches, place them on a small stirrer, stir at a stirring speed of 9000 r / min for 8 min, mix the liquid after each stirring, and obtain stirring liquid B;

[0060] d. Pour 300 mL of stirring liquid B each time, place them on a small stirrer, stir at a stirring speed of 9000 r / min for 8 min, mix the liquid after each stirring, and obtain stirring liquid C;

[0061] e. Pour 300 mL of stirring liquid C in batches, place them on a small stirrer, stir at a stirring speed of 9000 r / min for 8 min, mix the liquid after each stirring, and obtain stirring liquid D;

[0062] f. Filter the stirred liquid D through a 300-mesh filter cloth to obtain a filtrate;

[0063] g. Add the above-mentioned milk powder in parts by weight to the filtrate obtained in step f, stir until the milk powder is completely dissolved, put it on a magnetic stirrer, stir it at a stirring speed of 900 r / min, and stir it for 15 min to obtain stirring liquid E;

[0064] h. Add the above-mentioned red pigment in parts by weight to the stirred liquid E, stir evenly, and let it stand for 45 hours.

[0065] Embodiment 4:

[0066] The women's menstrual blood simulation fluid is made of the following raw materials by weight:

[0067] 868.45g of tertiary water, 40g of sodium chloride, 1g of sodium citrate, 3g of sodium hydroxide, 5g of potassium carbonate, 1g of glycerol, 1g of sodium carboxymethyl cellulose, 75g of milk powder, 0.025g of fatty acid sorbitan, 0.025g of lauric acid, 0.5g of lactic acid, and 2g of red pigment.

[0068] The method for preparing the simulated menstrual blood liquid comprises the following steps:

[0069] a. Take the above weight of sodium chloride, sodium citrate, sodium hydroxide, potassium carbonate, glycerol, sodium carboxymethyl cellulose, fatty acid shamnoic acid, lauric acid and lactic acid, pour into a beaker, stir and mix evenly with a glass rod;

[0070] b. Take the above weight of grade tertiary water and slowly pour it into the above beaker, stirring with a glass rod while pouring; then put the beaker on a magnetic stirrer, stir at a stirring speed of 600r / min for 15min to obtain solution A;

[0071] c. Pour 220 mL of stirring liquid A in batches, place them on a small stirrer, stir at a stirring speed of 11000 r / min for 5 min, mix the liquid after each stirring, and obtain stirring liquid B;

[0072] d. Pour 220 mL of stirring liquid B each time, place them on a small stirrer, stir at a stirring speed of 11000 r / min for 4 min, mix the liquid after each stirring, and obtain stirring liquid C;

[0073] e. Pour 220 mL of stirring liquid C in batches, place them on a small stirrer, stir at a stirring speed of 11000 r / min for 8 min, mix the liquid after each stirring, and obtain stirring liquid D;

[0074] f. Filter the stirred liquid D through a 300-mesh filter cloth to obtain a filtrate;

[0075] g. Add the above-mentioned protein powder in parts by weight to the filtrate obtained in step f, stir until the protein powder is completely dissolved, put it on a magnetic stirrer, use a stirring speed of 800 r / min, and stir it thoroughly for 15 min to obtain stirring solution E;

[0076] h. Add the above-mentioned red pigment in parts by weight to the stirred liquid E, stir evenly, and let it stand for 39 hours.

[0077] Embodiment 5:

[0078] The women's menstrual blood simulation fluid is made of the following raw materials by weight:

[0079] 870.4g of tertiary water, 40g of sodium sulfate, 5g of citric acid, 5g of phosphoric acid, 2g of gelatin, 75g of milk powder, 0.05g of sodium lauryl sulfate, 0.05g of glyceryl monostearate, 0.5g of lactic acid, and 2g of red pigment.

[0080] The method for preparing the simulated menstrual blood liquid comprises the following steps:

[0081] a. Take the above weight of sodium sulfate, citric acid, phosphoric acid, glycerol, sodium lauryl sulfate, glyceryl monostearate and lactic acid, pour them into a beaker, and stir with a glass rod to mix evenly;

[0082] b. Take the above weight of grade tertiary water and slowly pour it into the above beaker, stirring with a glass rod while pouring; then put the beaker on a magnetic stirrer, stir at a stirring speed of 600r / min for 15min to obtain solution A;

[0083] c. Pour 280 mL of stirring liquid A in batches, place them on a small stirrer, stir at a stirring speed of 15000 r / min for 4 min, mix the liquid after each stirring, and obtain stirring liquid B;

[0084] d. Pour 280 mL of stirring liquid B in batches, place them on a small stirrer, stir at a stirring speed of 15000 r / min for 8 min, mix the liquid after each stirring, and obtain stirring liquid C;

[0085] e. Pour 280 mL of stirring liquid C in batches, place them on a small stirrer, stir for 5 min at a stirring speed of 15000 r / min, mix the liquid after each stirring, and obtain stirring liquid D;

[0086] f. Filter the stirred liquid D through a 300-mesh filter cloth to obtain a filtrate;

[0087] g. Add the above-mentioned soybean milk powder in parts by weight to the filtrate obtained in step f, stir until the soybean milk powder is completely dissolved, put it on a magnetic stirrer, stir it at a stirring speed of 600 r / min, and stir it for 15 minutes to obtain stirring liquid E;

[0088] h. Add the above-mentioned red pigment in parts by weight to the stirred liquid E, stir evenly, and let it stand for 40 hours.

[0089] Example 6

[0090] The simulated menstrual blood fluid and the preparation method thereof are the same as those in Example 1, except that sodium dodecylbenzenesulfonate is used to replace sodium dodecyl sulfate and glyceryl monostearate in an equal weight ratio.

[0091] Example 7

[0092] The simulated menstrual blood liquid and its preparation method are the same as those in Example 2, except that glycerol is replaced by polyvinyl alcohol and gum arabic in an equal weight ratio.

[0093] The small mixer can be a soybean milk maker.

[0094] Experimental determination:

[0095] 1. Experimental materials:

[0096] 1. Standard solution: the standard synthetic test solution in Appendix G of the national standard (GB / T 22875-2008); 2. Human blood; 3. Women's menstrual blood simulation solution of each embodiment of the present invention.

[0097] 2. Experimental Methods

[0098] The pH, viscosity, density and surface tension indexes of the standard solution, human blood and the simulated menstrual blood solution of each embodiment of the present invention were tested respectively, wherein:

[0099] The pH value was determined using the method in the national standard GB 9724-2007 "General Rules for Determination of pH Value of Chemical Reagents";

[0100] The viscosity was determined using the method in the national standard GB / T 5561-2012 "Method for Determining Viscosity and Flow Properties of Surfactants Using Rotational Viscometer";

[0101] Density is determined using the method in national standard GB / T 22230-2008 "Determination of density of industrial liquid chemicals at 20°C";

[0102] The surface tension index is determined by the method in the national standard GB / T 22237-2008 "Determination of surface tension of surfactants".

[0103] 3. Experimental Results

[0104] The test results are shown in Table 1. By comparison, it can be seen that the pH value of the simulated menstrual blood solution prepared by the present invention is between 7.20±0.5 at room temperature, the viscosity is between 7.0±0.8mPa·s, and the density is between 1.06±0.05g / cm 2 The surface tension is between 40±4mN / m, which is closer to women's menstrual blood than the standard solution. It is used to measure the penetration and absorption properties of sanitary napkin products and high-absorbent resin materials. Compared with the existing standard solution, the test results are more instructive for actual production.

[0105] Table 1 Parameter performance comparison of the embodiment of the present invention, human blood and standard solution

[0106]

Claims

1. A menstrual blood simulating fluid, characterized by: Made from the following raw materials in parts by weight: 868.45 parts of tertiary water, 40 parts of sodium chloride, 1 part of sodium citrate, 3 parts of sodium hydroxide, 5 parts of potassium carbonate, 1 part of glycerol, 1 part of sodium carboxymethyl cellulose, 75 parts of milk powder, 0.025 parts of fatty acid scutellaria baicalensis, 0.025 parts of lauric acid, 0.5 parts of lactic acid, and 2 parts of red pigment; The method for preparing the simulated menstrual blood liquid comprises the following steps: a. Take the above-mentioned parts by weight of sodium chloride, sodium citrate, sodium hydroxide, potassium carbonate, glycerol, sodium carboxymethyl cellulose, fatty acid shan litan, lauric acid and lactic acid, pour them into a beaker, and stir and mix them evenly with a glass rod; b. Take the above-mentioned third-grade water in the above-mentioned parts by weight and slowly pour it into the above-mentioned beaker, stirring with a glass rod while pouring; then put the beaker on a magnetic stirrer, stir at a stirring speed of 600r / min for 15min to obtain solution A; c. Pour 220 mL of stirring liquid A in batches, place them on a small stirrer, stir for 5 min at a stirring speed of 11000 r / min, mix the liquid after each stirring, and obtain stirring liquid B; d. Pour 220 mL of stirring liquid B each time, place them on a small stirrer, stir for 4 min at a stirring speed of 11000 r / min, mix the liquid after each stirring, and obtain stirring liquid C; e. Pour 220 mL of stirring liquid C each time, place them on a small stirrer, stir for 8 min at a stirring speed of 11000 r / min, mix the liquid after each stirring, and obtain stirring liquid D; f. Filter the stirred liquid D through a 300-mesh filter cloth to obtain a filtrate; g. Add the above-mentioned milk powder in parts by weight to the filtrate obtained in step f, stir until the milk powder is completely dissolved, put it on a magnetic stirrer, stir it at a stirring speed of 800 r / min, and stir it for 15 min to obtain stirring liquid E; h. Add the above-mentioned red pigment in the stirred liquid E, stir evenly, and let stand for not less than 39 hours to obtain; The prepared simulated menstrual blood solution has a pH value of 7.5, a viscosity of 7.4 mPa·s, and a density of 1.05 g / cm 2 , the surface tension is 42mN / m.

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