Collagen template biomimetic mineralized zinc-strontium doped hydroxyapatite and preparation method thereof

Abstract

The invention particularly relates to collagen template biomimetic mineralized zinc-strontium doped hydroxyapatite and a preparation method thereof, and belongs to the technical field of biological materials, and the method comprises the following steps: obtaining a collagen solution; adding a calcium source, a zinc source, a strontium source and a phosphorus source into the collagen solution to obtain a precursor mixed solution; adjusting the pH value of the precursor mixed solution to obtain a solution to be reacted; carrying out stirring reaction on the solution to be reacted to obtain a hydroxyapatite solution; the preparation method comprises the following steps: preparing a hydroxyapatite solution, freeze-drying the hydroxyapatite solution to obtain biomimetic mineralized zinc-strontium doped hydroxyapatite, and assembling zinc-strontium ions and a collagen template into biomimetic hydroxyapatite, so that continuous release of the zinc-strontium ions can be realized, and the effects of programmed osteogenesis immunoregulation and bone formation promotion are realized through the released ions; the bone repair material is a preferable bone repair substitute material and has a wide application prospect.

Description

technical field

[0001] The invention belongs to the technical field of biomaterials, in particular to a collagen template bionic mineralization zinc strontium doped hydroxyapatite and a preparation method thereof. Background technique

[0002] The immune system and the skeletal system share many cytokines, receptors, signaling molecules, and transcription factors, and immune cells play a key role in bone homeostasis, so the immune system and the skeletal system are closely related. After the biomaterial is implanted, the body will inevitably have an immune response, and the development of biomaterials that can regulate the immune response has very important practical significance for the repair of damaged bone tissue. Contents of the invention

[0003] The purpose of this application is to provide a collagen template biomimetic mineralized zinc strontium doped hydroxyapatite and its preparation method, so as to solve the problem of immune response in the body after biomate...

Examples

preparation example Construction

[0042] According to a typical embodiment of the present invention, a method for preparing collagen template biomimetic mineralization zinc strontium doped hydroxyapatite is provided, the method comprising:

[0043] S1. Obtain a collagen solution;

[0044] As an optional implementation, obtain a collagen solution, specifically comprising:

[0045] The collagen is dissolved in the acidic solution to obtain a collagen solution.

[0046]As an optional embodiment, the collagen includes type I collagen, the acidic solution includes one of acetic acid solution, dilute hydrochloric acid solution and dilute nitric acid solution, and the pH value of the acidic solution is 2-4.

[0047] Control the pH value of the acidic solution to be 2-4 to dissolve type I collagen. If the value is too large, the unfavorable effect is that the collagen cannot be dissolved well. Templated co-assembly of hydroxyapatite.

[0048] S2. adding calcium source, zinc source, strontium source and phosphorus s...

Embodiment 1

[0066] A collagen template-zinc-strontium double ion co-assembled hydroxyapatite with programmed osteogenic immune regulation and osteogenesis is prepared by the following method:

[0067] (1) Weigh 0.4303g of collagen and dissolve it in 100mL of acetic acid solution with a pH of 3 to obtain a 0.4% (w / v) collagen solution;

[0068] (2) Take by weighing 2.13g calcium nitrate tetrahydrate, join in the aforementioned collagen protein solution, fully stir and dissolve;

[0069] (3) Zinc source, strontium source and phosphorus source solutions of 0.1mol / L are configured, and the specific zinc, strontium and phosphorus sources used are zinc nitrate hexahydrate, strontium nitrate and disodium hydrogen phosphate respectively in this embodiment;

[0070] (4) Add the zinc source, strontium source and phosphorus source solutions configured in step (3) to the solution in step (2), the volumes of which are 5 mL, 5 mL and 60 mL respectively, and fully stir and mix;

[0071] (5) configure t...

Embodiment 2

[0075] A collagen template-zinc-strontium double ion co-assembled hydroxyapatite with programmed osteogenic immune regulation and osteogenesis is prepared by the following method:

[0076] (1) Weigh 0.4303g of collagen and dissolve it in 100mL of acetic acid solution with a pH of 3 to obtain a 0.4% (w / v) collagen solution;

[0077] (2) Take by weighing 2.01g calcium nitrate tetrahydrate, join in the aforementioned collagen protein solution, fully stir and dissolve;

[0078] (3) Zinc source, strontium source and phosphorus source solutions of 0.1mol / L are configured, and the specific zinc, strontium and phosphorus sources used are zinc nitrate hexahydrate, strontium nitrate and disodium hydrogen phosphate respectively in this embodiment;

[0079] (4) Add the zinc source, strontium source and phosphorus source solutions configured in step (3) to the solution in step (2), the volumes are respectively 10mL, 5mL and 60mL, fully stir and mix;

[0080] (5) configure the NaOH solutio...

technical field

[0001] The invention belongs to the technical field of biomaterials, in particular to a collagen template bionic mineralization zinc strontium doped hydroxyapatite and a preparation method thereof. Background technique

[0002] The immune system and the skeletal system share many cytokines, receptors, signaling molecules, and transcription factors, and immune cells play a key role in bone homeostasis, so the immune system and the skeletal system are closely related. After the biomaterial is implanted, the body will inevitably have an immune response, and the development of biomaterials that can regulate the immune response has very important practical significance for the repair of damaged bone tissue. Contents of the invention

[0003] The purpose of this application is to provide a collagen template biomimetic mineralized zinc strontium doped hydroxyapatite and its preparation method, so as to solve the problem of immune response in the body after biomate...

Examples

preparation example Construction

[0042] According to a typical embodiment of the present invention, a method for preparing collagen template biomimetic mineralization zinc strontium doped hydroxyapatite is provided, the method comprising:

[0043] S1. Obtain a collagen solution;

[0044] As an optional implementation, obtain a collagen solution, specifically comprising:

[0045] The collagen is dissolved in the acidic solution to obtain a collagen solution.

[0046]As an optional embodiment, the collagen includes type I collagen, the acidic solution includes one of acetic acid solution, dilute hydrochloric acid solution and dilute nitric acid solution, and the pH value of the acidic solution is 2-4.

[0047] Control the pH value of the acidic solution to be 2-4 to dissolve type I collagen. If the value is too large, the unfavorable effect is that the collagen cannot be dissolved well. Templated co-assembly of hydroxyapatite.

[0048] S2. adding calcium source, zinc source, strontium source and phosphorus s...

Embodiment 1

[0066] A collagen template-zinc-strontium double ion co-assembled hydroxyapatite with programmed osteogenic immune regulation and osteogenesis is prepared by the following method:

[0067] (1) Weigh 0.4303g of collagen and dissolve it in 100mL of acetic acid solution with a pH of 3 to obtain a 0.4% (w / v) collagen solution;

[0068] (2) Take by weighing 2.13g calcium nitrate tetrahydrate, join in the aforementioned collagen protein solution, fully stir and dissolve;

[0069] (3) Zinc source, strontium source and phosphorus source solutions of 0.1mol / L are configured, and the specific zinc, strontium and phosphorus sources used are zinc nitrate hexahydrate, strontium nitrate and disodium hydrogen phosphate respectively in this embodiment;

[0070] (4) Add the zinc source, strontium source and phosphorus source solutions configured in step (3) to the solution in step (2), the volumes of which are 5 mL, 5 mL and 60 mL respectively, and fully stir and mix;

[0071] (5) configure t...

Embodiment 2

[0075] A collagen template-zinc-strontium double ion co-assembled hydroxyapatite with programmed osteogenic immune regulation and osteogenesis is prepared by the following method:

[0076] (1) Weigh 0.4303g of collagen and dissolve it in 100mL of acetic acid solution with a pH of 3 to obtain a 0.4% (w / v) collagen solution;

[0077] (2) Take by weighing 2.01g calcium nitrate tetrahydrate, join in the aforementioned collagen protein solution, fully stir and dissolve;

[0078] (3) Zinc source, strontium source and phosphorus source solutions of 0.1mol / L are configured, and the specific zinc, strontium and phosphorus sources used are zinc nitrate hexahydrate, strontium nitrate and disodium hydrogen phosphate respectively in this embodiment;

[0079] (4) Add the zinc source, strontium source and phosphorus source solutions configured in step (3) to the solution in step (2), the volumes are respectively 10mL, 5mL and 60mL, fully stir and mix;

[0080] (5) configure the NaOH solutio...

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