A composite microbial fertilizer for promoting the growth and development of pepper and its application

Abstract

The present invention provides a composite microbial fertilizer for promoting the growth and development of peppers, and belongs to the technical field of composite microbial fertilizer research and development. The composite microbial fertilizer of the present application is composed of Bacillus subtilis, Bacillus velez, Bacillus elongatus and Trichoderma harzianum in a volume ratio of 1:1:1:1. Using the composite microbial fertilizer of the present invention to plant peppers not only reduces the cost of preparing the microbial solution, but also improves the soil quality, increases the activity of urease, sucrase, acid phosphatase and peroxidase in the rhizosphere soil of peppers, and increases the soil alkaline nitrogen and available phosphorus content and the yield of peppers.

Description

Technical Field

[0001] The invention relates to the technical field of research and development of composite microbial fertilizers, and in particular to a composite microbial fertilizer for promoting the growth and development of peppers and an application thereof. Background Art

[0002] The most essential and significant feature of microbial fertilizer is that it is a living, active fertilizer. It is a new generation of high-performance fertilizer that is lower in cost, better in fertilizer efficiency, higher in yield, and better in quality than traditional agricultural fertilizers. It can also inhibit soil degradation, improve soil structure, inhibit soil-borne diseases, and has complete functions. Microbial fertilizer can be widely used in various crops, fruit trees, vegetables, tobacco, tea, flowers, and lawns. Since microbial fertilizer contains living plant growth-promoting bacteria, and different growth-promoting bacteria have different growth-promoting properties, microbial fertilizer has high requirements for the type of growth-promoting bacteria. In addition, different plant roots have different core microbial groups, which requires the use of microbial fertilizer to be targeted. At present, the most commonly used composite microbial fertilizer is EM bacteria, which is mostly a general-purpose fertilizer, including Bacillus, yeast, and lactic acid bacteria. However, EM fertilizer lacks growth-promoting fungi, and fungi play a key role in the rhizosphere of plants, such as arbuscular mycorrhizal fungi and Trichoderma harzianum.

[0003] Chinese Patent 202010389954.1 provides a composite microbial fertilizer for pepper cultivation, including beneficial microorganisms such as Lactobacillus acidophilus and Bacillus subtilis, as well as trace elements, etc. The patent prepares a composite microbial fertilizer by optimizing the fermentation medium, which can promote the growth and development of peppers and increase profits, but the fertilizer has few types of growth-promoting bacteria.

[0004] Chinese patent 202211495212.2 mixes Bacillus colloids with Trichoderma harzianum. Bacillus colloids has the functions of nitrogen fixation, phosphorus dissolution and potassium dissolution. At the same time, the addition of Trichoderma harzianum also solves the problem of invasion by various pathogens. However, the animal manure used in the preparation process of this bacterial fertilizer has the problem of excessive antibiotics.

[0005] Chinese patent 201711073546.X granulates Bacillus cereus, Bacillus megaterium, Rhodopseudomonas palustris, Candida utilis and Trichoderma harzianum and coats them, which improves the fertilizer utilization rate. However, the preparation cost of the compound fertilizer increases and the process is complicated.

[0006] Therefore, we proposed a preparation method of a composite microbial fertilizer comprising growth-promoting bacteria and fungi, which can effectively increase pepper yield and improve soil, and applied it to pepper cultivation. Summary of the invention

[0007] The purpose of the present invention is to provide a composite microbial fertilizer for promoting the growth and development of peppers and its application. The plant growth-promoting bacteria isolated and screened are from the rhizosphere soil of peppers, can be well colonized in the roots of peppers, regulate microbial communities, promote the growth of peppers, and increase pepper yields.

[0008] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0009] The invention provides a composite microbial fertilizer for promoting the growth and development of peppers. The composite microbial fertilizer comprises Bacillus subtilis, Bacillus velez, Bacillus elongatus and Trichoderma harzianum.

[0010] Furthermore, the volume ratio of the Bacillus subtilis, Bacillus velezensii, Bacillus elongatus and Trichoderma harzianum is 1:1:1:1.

[0011] Furthermore, the viable counts of the Bacillus subtilis, Bacillus velezii, Bacillus elongatus and Trichoderma harzianum are 1 billion to 5 billion / mL.

[0012] The deposit number of the Bacillus subtilis is CICC10732, the deposit number of the Bacillus velez is CICC22947, the deposit number of the Bacillus elongatus is CICC24620, and the deposit number of the Trichoderma harzianum is CICC13010.

[0013] The invention also provides an application of a composite microbial fertilizer for promoting the growth and development of peppers in pepper planting.

[0014] The present invention also has the following beneficial effects: (1) reducing the cost of preparing microbial culture liquid; (2) improving soil quality and increasing the activities of urease, sucrase, acid phosphatase and peroxidase; (3) increasing the content of soil alkaline nitrogen and available phosphorus; and (4) increasing the yield of pepper. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a microscope image of the strains in Example 1; from left to right, they are Bacillus subtilis, Bacillus velez, Bacillus elongatus and Trichoderma harzianum;

[0016] Figure 2 The stem diameters of each treatment in Example 1 after 28 days, 56 days and 112 days of using the composite microbial solution;

[0017] Figure 3 The plant heights of each treatment in Example 1 after using the composite microbial solution for 28 days, 56 days and 112 days;

[0018] Figure 4The fresh weight of the plants in each treatment of Example 1 after using the composite microbial solution for 28 days, 56 days and 112 days;

[0019] Figure 5 Chlorophyll content of each treatment in Example 1 after 28 days, 56 days and 112 days of using the composite microbial solution;

[0020] Figure 6 The SPAD values ​​of each treatment in Example 1 after using the composite microbial solution for 28 days, 56 days and 112 days;

[0021] Figure 7 The root activity of each treatment in Example 1 after using the composite microbial solution for 28 days, 56 days and 112 days;

[0022] Figure 8 The sucrase activity of pepper rhizosphere soil in each treatment of Example 1 after using the composite microbial solution for 28 days, 56 days and 112 days;

[0023] Fig. 9 Urease activity in the rhizosphere soil of pepper in each treatment of the embodiment after using the composite microbial solution for 28 days, 56 days and 112 days;

[0024] Fig.10 The peroxidase activity of pepper rhizosphere soil in each treatment of Example 1 after using the composite microbial solution for 28 days, 56 days and 112 days;

[0025] Fig.11 The acid phosphatase activity of pepper rhizosphere soil in each treatment of Example 1 after using the composite microbial solution for 28 days, 56 days and 112 days;

[0026] Fig.12 The alkaline nitrogen content in the soil of each treatment in Example 1 after using the composite microbial solution for 28 days, 56 days and 112 days;

[0027] Fig.13 The available phosphorus content in the soil of each treatment in Example 1 after using the composite microbial solution for 28 days, 56 days and 112 days;

[0028] Fig.14 This is the pepper yield of each treatment in Example 1 after 112 days of using the composite microbial solution. DETAILED DESCRIPTION

[0029] The embodiments of the present invention are described in detail below, and the embodiments are intended to explain the present invention and should not be construed as limiting the present invention. If specific techniques or conditions are not specified in the embodiments, they are carried out according to the techniques or conditions described in the literature in this area or according to the product specifications. If the manufacturers of reagents or instruments are not specified, they are all conventional products that can be obtained commercially.

[0030] Bacillus subtilis CICC10732, Bacillus velez CICC22947, Bacillus elongatus with the deposit number of CICC24620, and Trichoderma harzianum CICC13010 were all purchased.

[0031] Example 1

[0032] (1) Pepper seeds were placed in a water bath at 50°C for 15 min, then continued to be soaked at room temperature for 8 h and placed in the dark for 3 days. After the seeds turned white, they were sown in seedling trays (the matrix soil was peat soil: perlite: vermiculite = 2:1:1). After the seeds germinated in the seedling trays to the point where they had three leaves and one heart, seedlings with uniform growth were selected for transplanting.

[0033] (2) Plant pepper seedlings in plastic pots with a bottom diameter of 10.5 cm and a height of 13.5 cm, one pepper seedling per pot;

[0034] (3) Preparation of bacterial solution: Observe the growth of Bacillus subtilis, Bacillus velezensis, Bacillus elongatus, and Trichoderma harzianum under a microscope (results are shown in Table 1). Figure 1 As shown). Bacillus subtilis CICC10732, Bacillus Velezii CICC22947 and Bacillus elongatus CICC24620 on Luria-Bertani (LB) solid medium were picked up with a bacterial loop and placed in conical flasks containing 200 mL LB liquid medium, and Trichoderma harzianum CICC13010 on PDA solid medium was picked up and placed in a conical flask containing 200 mL PDA liquid medium. After constant temperature shaking at 180 rpm and 37°C (Trichoderma harzianum at 28°C) for 24 h, the bacterial solution was obtained, and the bacterial solution was centrifuged at 8000 rpm and 4°C. Each bacterial solid was diluted with sterile distilled water until the number of viable bacteria was 1 billion / mL, and mixed in a volume ratio of 1:1:1:1 to obtain a composite microbial fertilizer.

[0035] (5) Use compound microbial fertilizer once every seven days, 50 mL per tree each time, and add an equal amount of sterile distilled water to the control group.

[0036] (6) The growth and soil conditions of peppers after 28 days, 56 days, and 112 days of using the composite microbial solution, as well as the yield of peppers after 122 days, were statistically analyzed. The results are as follows Figures 2~14 As shown (CK is the control group; KC is Bacillus subtilis fertilizer, BLS is Bacillus velezii fertilizer, LAS is Bacillus elongatus fertilizer, HC is Trichoderma harzianum fertilizer, and BF is compound microbial fertilizer).

[0037] like Figures 2~14As shown in the figure, after 112 days of cultivation, the height, stem thickness and fresh weight of pepper plants in the five experimental groups were significantly higher than those in the control group, and the effect of the compound bacterial fertilizer was better than that of the single bacterial fertilizer; the root activity, SPAD value and chlorophyll content of pepper plants in the five experimental groups were also significantly higher than those in the control group, especially the total chlorophyll content. After 112 days, it was significantly higher than that of the control group and the single bacterial fertilizer. For root activity, the compound bacterial fertilizer had the best effect, followed by Bacillus subtilis, and then Bacillus elongatus. This shows that the microbial bacterial solution of the experimental group can promote the growth of pepper plants, among which the microbial bacterial solution of the four growth-promoting bacteria has the best effect, which can promote the growth of pepper roots, increase yield, and better utilize nutrients in the soil.

[0038] Soil samples were obtained at three stages. After 112 days of fertilizer application, each experimental group was able to increase the content of alkaline nitrogen and available phosphorus in the soil, and increase the activity of urease, sucrase, acid phosphatase and peroxidase in the soil. Among them, the microbial fertilizer with four growth-promoting bacteria had the best effect. This shows that the microbial compound fertilizer can improve soil quality and increase the utilization rate of available nitrogen and phosphorus in the soil.

[0039] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composite microbial fertilizer for promoting the growth and development of pepper, characterized in that: The composite microbial fertilizer comprises Bacillus subtilis, Bacillus velez, Bacillus elongatus and Trichoderma harzianum; The volume ratio of the Bacillus subtilis, Bacillus velez, Bacillus elongatus and Trichoderma harzianum is 1:1:1:1; The deposit number of the Bacillus subtilis is CICC10732, the deposit number of the Bacillus velez is CICC22947, the deposit number of the Bacillus elongatus is CICC24620, and the deposit number of the Trichoderma harzianum is CICC13010.

2. The composite microbial fertilizer according to claim 1, characterized in that: The viable bacterial counts of the Bacillus subtilis, Bacillus velezii, Bacillus elongatus and Trichoderma harzianum are 1 billion to 5 billion / mL.

3. Application of the composite microbial fertilizer for promoting pepper growth and development as claimed in claim 1 in pepper planting.

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