A plasma adsorption filter and application

Abstract

The present invention provides a plasma adsorption filter and application, the plasma adsorption filter comprises a filter and an adsorber, the filter and the adsorber are laid flat and connected by bonding, the filter is a polyethersulfone membrane, and the adsorber is an aldehyde cellulose membrane; the thickness of the polyethersulfone membrane is 25-30 μm, and the thickness of the aldehyde cellulose membrane is 0.01-100 μm. The plasma adsorption filter provided by the present invention can efficiently remove toxins and metabolites in plasma, enhance the selectivity and specificity of plasma, specifically adsorb interleukin-8 in plasma, has good biocompatibility, and has good application prospects.

Description

Technical Field

[0001] The invention relates to the technical field of membrane materials, in particular to a plasma adsorption filter and application thereof. Background Art

[0002] The plasma adsorption filter is a medical device used for blood purification, mainly used to treat various diseases caused by the accumulation of toxins, inflammatory mediators or metabolites in the body. Its working principle is to remove these harmful substances from the plasma through adsorption technology to achieve the purpose of purifying the blood. It can be used to remove bilirubin, ammonia and other toxic substances in the blood and relieve the symptoms of liver failure. It can also reduce inflammatory reactions and improve patient prognosis by removing bacterial toxins and inflammatory mediators.

[0003] Interleukin 8 (IL-8) is a chemokine that is primarily secreted by macrophages and epithelial cells. IL-8 plays an important role in the immune system, especially in attracting and activating neutrophils. It has many biological functions, including Chemotaxis: IL-8 is a potent chemokine that attracts neutrophils, T lymphocytes, and eosinophils to sites of inflammation. Activation: IL-8 can activate neutrophils, enhancing their phagocytic ability and bactericidal activity. Inflammatory response: IL-8 plays a key role in the inflammatory response, and excessive IL-8 can lead to chronic inflammation and tissue damage.

[0004] In some clinical treatment scenarios, plasma adsorption filters may be used to regulate the levels of inflammatory mediators such as interleukin 8 to control inflammatory responses. For example, in severe sepsis or autoimmune diseases, removing excess IL-8 through plasma adsorption filters may help relieve symptoms and improve the condition.

[0005] However, the technical development of existing plasma adsorption filtration devices for application in this area still needs to be improved. Summary of the invention

[0006] The technical problem to be solved by the present invention is that the plasma adsorption filter device in the prior art is still unable to produce a specific adsorption effect on interleukin-8 in plasma.

[0007] In order to solve the above technical problems, in the first aspect, the present invention provides a plasma adsorption filter, which includes a filter and an adsorber, and the filter and the adsorber are laid flat and connected by bonding, the filter is a polyethersulfone membrane, and the adsorber is an aldehyde cellulose membrane; the thickness of the polyethersulfone membrane is 25~30μm, and the thickness of the aldehyde cellulose membrane is 0.01~100μm.

[0008] The plasma adsorption filter provided by the present invention can achieve chemical adsorption of interleukins through the Schiff base chemical reaction between the aldehyde group on the membrane and the interleukin amine group, and is not affected by interfering ions during the adsorption process. At the same time, the cellulose material itself has the characteristics of green degradability, low toxicity, acid and alkali resistance, and stable structure. Combined with the use of polyethersulfone membrane, the combination of the two can further enhance the strength of the plasma adsorption filter, while improving biocompatibility, reducing adverse reactions caused by blood filtration, and improving the effect of plasma adsorption filtration, especially for the adsorption effect of interleukin-8.

[0009] The thickness of the polyethersulfone membrane is 25-30 μm, for example, 25 μm, 26 μm, 27 μm, 28 μm, 29 μm or 30 μm.

[0010] Preferably, the aldehyde-based cellulose membrane is a dialdehyde-modified cellulose membrane.

[0011] In the present invention, the more aldehyde groups there are on the cellulose main chain, the better the adsorption effect on interleukin-8. Moreover, the conditions of the entire adsorption process are relatively mild.

[0012] Preferably, the dialdehyde-modified cellulose membrane is prepared by the following method:

[0013] Cellulose was used as a substrate and sodium periodate was used for oxidation reaction to obtain a dialdehyde-modified cellulose membrane.

[0014] The reaction process of the invention mainly involves the selective oxidation reaction of the secondary carbon hydroxyl groups at the C2 and C3 positions under the action of sodium periodate, so that the cellulose structural unit is ring-opened to generate dialdehyde cellulose.

[0015] The thickness of the aldehyde cellulose film is 0.01-100 μm, for example, 0.01 μm, 1 μm, 10 μm, 20 μm, 40 μm, 60 μm, 80 μm or 100 μm.

[0016] In the present invention, the thickness of the cellulose aldehyde membrane affects the effect of adsorption filtration. The main reason is due to the principle of depth filtration. When the thickness is thicker, the plasma passes through the membrane for filtration and the more functional groups respond, the better the adsorption effect. When the thickness is thinner, the filtration is relatively less and the adsorption effect is reduced. In the present invention, preferably within the above range, the effect is best. When the thickness of the membrane is too large, it will cause an increase in the transmembrane pressure difference, affecting the filtration speed.

[0017] Preferably, the filter has 1 to 3 layers, for example, 1 layer, 2 layers, 3 layers, etc.

[0018] Preferably, the adsorber has 1 to 3 layers, for example, 1 layer, 2 layers, 3 layers, etc.

[0019] Preferably, the filter and the adsorber are bonded alternately or bonded sequentially.

[0020] In the present invention, alternate bonding connection means that a multilayer structure of filter, adsorber, filter, and adsorber are alternately bonded and arranged; sequential bonding connection means that filter, filter, adsorber, and adsorber are sequentially bonded and arranged.

[0021] In a second aspect, the present invention provides an application of a plasma adsorption filter in adsorbing interleukin-8 in plasma.

[0022] The implementation of the present invention has the following beneficial effects:

[0023] The plasma adsorption filter provided by the present invention can efficiently remove toxins and metabolites in plasma, enhance the selectivity and specificity of plasma, specifically adsorb interleukin-8 in plasma, and has good biocompatibility.

[0024] In addition, the plasma adsorption filter is easy to functionalize, and the aldehyde groups on the surface of the aldehyde cellulose membrane provide convenience for further functionalization. Through technical extension, the membrane can be further multifunctionalized by introducing other functional groups (such as amine groups, carboxyl groups, etc.) to meet different application requirements and expand its application range.

[0025] Since the pore size structure of cellulose aldehyde membrane can be adjusted through the preparation process, it can meet the separation requirements of molecules of different sizes. It is biodegradable and will not pollute the environment after use, which is in line with the concept of green environmental protection. DETAILED DESCRIPTION

[0026] The specific embodiments of the present invention are described in detail below. It should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0027] The endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed in this article.

[0028] Example 1

[0029] This embodiment provides a plasma adsorption filter, which has four layers of filter, adsorber, filter and adsorber in sequence. The filter is a 29 μm thick polyethersulfone membrane, and the adsorber is a 60 nm dialdehyde-modified cellulose membrane.

[0030] Example 2

[0031] This embodiment provides a plasma adsorption filter, which has three layers of structure, namely, a filter, an adsorber and a filter. The filter is a 26 μm thick polyethersulfone membrane, and the adsorber is a 40 μm dialdehyde-modified cellulose membrane.

[0032] Example 3

[0033] This embodiment provides a plasma adsorption filter, which has three layers of structure, namely, an adsorber, a filter and an adsorber, wherein the filter is a 28 μm thick polyethersulfone membrane and the adsorber is an 80 μm dialdehyde-modified cellulose membrane.

[0034] Example 4

[0035] This embodiment provides a plasma adsorption filter, which has four layers of filter, filter, adsorber and adsorber in sequence. The filter is a 30 μm thick polyethersulfone membrane, and the adsorber is a 90 μm dialdehyde-modified cellulose membrane.

[0036] Example 5

[0037] The plasma adsorption filter provided in this embodiment is the same as that in embodiment 1, except that the cellulose membrane modified with dialdehyde groups is different. The aldehyde content of the cellulose membrane modified with dialdehyde groups is reduced by half. That is, during the preparation process, the amount of sodium periodate used is reduced by half.

[0038] Comparative Example 1

[0039] This comparative example used only one filter layer.

[0040] Comparative Example 2

[0041] This comparative example uses only one layer of adsorber.

[0042] Comparative Example 3

[0043] The difference between this comparative example and Example 1 is that the adsorber in this comparative example is not modified with aldehyde groups, and only ordinary cellulose membrane is used.

[0044] The plasma filtration adsorbers of the above-mentioned embodiments and comparative examples were subjected to performance tests to test the adsorption effects. The specific results are shown in Table 1.

[0045] Test steps:

[0046] The plasma containing interleukin-8 (concentration of 252.1 pg / mL) was filtered once through Jiangxia Company's plasma filtration membrane (3M, 1 filter disc with a diameter of 4.7 cm) at a filtration rate of 1.1 mL / min; the filtration pressure was maintained within 100 kPa until the plasma became clear.

[0047] The plasma filtered through the Jiangxia plasma filtration membrane was used for filtration through the adsorption filter. The adsorption filter membrane had a diameter of 47 mm and was attached to the bottom of the Jiangxia filter membrane (1 piece). The filtration rate was 1.1 mL / min and the amount of plasma used was 10 mL.

[0048] After the plasma has gone through the above filtration process, the concentration of interleukin-8 in the plasma before and after filtration is tested by chemiluminescence method.

[0049] Table 1

[0050]

[0051] It can be seen from the data of the examples of the present invention and the comparative examples that: (1) the content of aldehyde groups affects the adsorption effect, and the adsorption layer with a high aldehyde group content can completely remove interleukins in plasma; (2) the thickness of the adsorption layer is positively correlated with the adsorption amount; (3) the adsorption effect of a single polysulfone membrane or an unmodified cellulose membrane on interleukins is limited.

[0052] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A use of a plasma adsorption filter for adsorbing interleukin-8 in plasma, characterized in that: The plasma adsorption filter comprises a filter and an adsorber, the filter and the adsorber are laid flat and connected by bonding, the filter is a polyethersulfone membrane, and the adsorber is an aldehyde-based cellulose membrane; the thickness of the polyethersulfone membrane is 25-30 μm, and the thickness of the aldehyde-based cellulose membrane is 0.01-100 μm; the aldehyde-based cellulose membrane is a cellulose membrane modified by dialdehyde groups.

2. Use of the plasma adsorption filter according to claim 1 in adsorbing interleukin-8 in plasma, characterized in that: The dialdehyde-modified cellulose membrane is prepared by the following method: Cellulose was used as the substrate and sodium periodate was used for oxidation reaction to obtain a dialdehyde-modified cellulose membrane.

3. Use of the plasma adsorption filter according to claim 1 in adsorbing interleukin-8 in plasma, characterized in that: The number of layers of the filter is 1 to 3.

4. Use of the plasma adsorption filter according to claim 1 for adsorbing interleukin-8 in plasma, characterized in that: The adsorber has 1 to 3 layers.

5. Use of the plasma adsorption filter according to claim 1 for adsorbing interleukin-8 in plasma, characterized in that: The filter and the adsorber are bonded alternately or sequentially.

Images