Cracking device for automatic nucleic acid extraction

Abstract

The invention relates to a cracking device for automatic nucleic acid extraction, which comprises a cracking turntable, the cracking turntable is provided with an inner disc body and an outer disc body, the outer disc body is annularly arranged on the periphery of the inner disc body, the outer disc body and the inner disc body are respectively provided with a plurality of sample initial holes and a plurality of sample treatment holes which are in one-to-one correspondence, the outer disc body and the inner disc body can respectively rotate along a first direction and a second direction which are opposite to each other; the cracking rotary disc further comprises a disc cover, the disc cover covers the multiple sample initial holes and the multiple sample treatment holes, and a second sample discharging station, a cover sealing and sample separating station, a sample feeding station, a cover opening station and a first sample feeding station are sequentially arranged on the disc cover in the first direction; a second sample feeding station, a liquid injection station, a heating station and a first sample discharging station are sequentially arranged on the disc cover in the second direction; and a liquid injection pipe of the liquid injection mechanism is positioned above the cracking turntable and is vertically opposite to the liquid injection station. The technical problems of complex structure, cumbersome splitting process and low speed of the splitting device in nucleic acid extraction are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid extraction, and in particular to a lysis device for automatic nucleic acid extraction. Background Art

[0002] Nucleic acids are divided into deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is mainly concentrated in the cell nucleus (mitochondria and chloroplasts), while RNA is mainly distributed in the cytoplasm. Nucleic acids, as the material basis of gene expression, are the main object of molecular biology research. Whether it is the structure or function of nucleic acids, it is first necessary to release the nucleic acids in the sample to be tested from the cells, and further separate the released nucleic acids from other impurities in the cells to obtain purer nucleic acids. This process is called nucleic acid extraction and purification. Nucleic acid extraction provides the basis for a large number of extensive research and applications.

[0003] There are three main types of nucleic acid extraction methods: solution extraction, column extraction, and magnetic bead purification. The most commonly used method is magnetic bead purification. Figure 1 As shown, a lysis solution is added to the sample tube containing the sample to release the nucleic acid in the cell nucleus; then magnetic beads are added to the sample tube so that the nucleic acid is adsorbed on the magnetic beads, and the magnetic beads adsorbed with the nucleic acid are enriched on one side of the sample tube through a magnet; then, the waste liquid in the sample tube is removed and a cleaning solution is added to wash the nucleic acid; then, the waste liquid after washing the nucleic acid is removed and an elution solution is added to separate the nucleic acid from the magnetic beads; finally, the magnetic beads are removed to obtain purified nucleic acid. The whole process can be simplified as: lysis, washing, and elution.

[0004] At present, many types of nucleic acid extractors have been developed based on the principle of magnetic bead purification, which are used to extract and purify nucleic acids from samples to be tested. Among them, the types of nucleic acid extractors include multi-sample simultaneous extraction type, single sample separate extraction type and multi-sample on-demand extraction type. Nucleic acid extraction instruments that can extract nucleic acids from multiple samples simultaneously can complete the nucleic acid extraction of multiple samples at one time, but they need to collect a sufficient number of samples before they can start running, and cannot achieve on-demand testing; although nucleic acid extraction instruments that can extract single samples do not need to collect a sufficient number of samples, their processing capacity is limited when faced with a situation where the sample volume is too large, and they cannot quickly complete the nucleic acid extraction; and nucleic acid extraction instruments that can be picked up on demand from multiple samples, on the one hand, can achieve on-demand sampling of samples without waiting for the collection of a sufficient number of samples, and on the other hand, can also be used in situations where the sample volume is too large, and have good development prospects. However, the existing technology of on-demand sampling nucleic acid extraction instruments is not yet mature, and the development of nucleic acid extraction instruments is still in its early stages, and there are many problems. For example, when the sample to be tested is subjected to nucleic acid lysis treatment, the lysis device has a complex structure and a large size, resulting in a cumbersome and slow lysis process, as well as problems such as excessive cost. No effective solution has yet been given.

[0005] Therefore, the present invention proposes a lysis device for automated nucleic acid extraction to overcome the defects of the prior art. Summary of the invention

[0006] The object of the present invention is to provide a lysis device for automated nucleic acid extraction, which can realize the on-demand collection of samples to be tested and accelerate the lysis rate of nucleic acid extraction. At the same time, when applied to scenarios with huge sample volumes, the samples to be tested will not be unable to be processed in time. In addition, the design of the device is more streamlined, the volume is reduced, the cost is lower, and the streamlined structure further simplifies the operation process, improves the lysis rate, and has a better use effect.

[0007] The purpose of the present invention can be achieved by adopting the following scheme:

[0008] The present invention provides a lysis device for automated nucleic acid extraction, the lysis device for automated nucleic acid extraction comprising:

[0009] A lysis turntable, the lysis turntable comprising an inner disk body and an outer disk body, the outer disk body being arranged around the outer circumference of the inner disk body, the outer disk body being provided with a plurality of sample initial holes for accommodating sample tubes at intervals along its circumference, the inner disk body being provided with a plurality of sample processing holes for accommodating the sample tubes at intervals along its circumference, along the radial direction of the lysis turntable, the plurality of sample initial holes correspond to the plurality of sample processing holes one by one, the outer disk body and the inner disk body being respectively rotatable in a first direction and a second direction, the first direction being opposite to the second direction;

[0010] The lysis turntable further comprises a disk cover, the disk cover is located above the inner disk body and the outer disk body, and the disk cover covers the plurality of sample initial holes and the plurality of sample processing holes, and the disk cover is provided with a second sample discharging station, a capping and sample separation station, a sample loading station, a cover opening station and a first sample loading station in sequence along the first direction, and the distances between the second sample discharging station, the capping and sample separation station, the sample loading station, the cover opening station and the first sample loading station and the central axis of the outer disk body are all equal to the distance between the sample initial holes and the central axis of the outer disk body; the disk cover is provided with a second sample loading station, a liquid injection station, a heating station and a first sample discharging station in sequence along the second direction, and the distances between the second sample loading station, the liquid injection station, the heating station and the first sample discharging station and the central axis of the inner disk body are all equal to the distance between the sample processing holes and the central axis of the inner disk body;

[0011] The liquid injection mechanism comprises a liquid injection tube for outputting magnetic bead lysis solution, and the liquid injection tube is located above the lysis turntable and vertically opposite to the liquid injection station.

[0012] In a preferred embodiment of the present invention, when the outer disk body rotates along the first direction, the sample tube containing the sample to be lysed is placed from the sample loading station into any of the sample initial holes vertically opposite thereto, and the sample tube is uncapped when the sample tube rotates with the outer disk body to be vertically opposite to the uncapping station;

[0013] When the sample tube rotates along the first direction with the outer disk to be vertically opposite to the first sample injection station, the sample tube is transferred from the sample initial hole to any of the sample processing holes vertically opposite to the second sample injection station.

[0014] In a preferred embodiment of the present invention, when the sample tube rotates along the second direction with the inner disk to be vertically opposite to the injection station, the magnetic bead lysis solution is injected into the sample tube through the injection tube, and the sample tube is heated at least when the sample tube rotates along the inner disk to a position vertically opposite to the heating station;

[0015] When the sample tube rotates along the second direction with the inner disk to be vertically opposite to the first sample output station, the sample tube is transferred from the first sample output station to any sample initial hole vertically opposite to the second sample output station.

[0016] In a preferred embodiment of the present invention, when the sample tube rotates along the first direction with the outer disk to be vertically opposite to the capping and sample separation station, the sample tube is subjected to a capping and sample removal process.

[0017] In a preferred embodiment of the present invention, the second sample discharging station, the capping and sample separation station, the sample loading station, the lid opening station, the first sample injection station, the second sample injection station, the liquid injection station, the heating station and the first sample discharging station are all through holes opened on the disk cover and are all located within the same half circumference of the disk cover.

[0018] In a preferred embodiment of the present invention, the first sample introduction station and the second sample introduction station are located on an extension line of the same radius of the disk cover;

[0019] And / or, the loading station and the heating station are located on an extension line of the same radius of the plate cover;

[0020] And / or, the second sample discharging station and the first sample discharging station are located on an extension line of the same radius of the disk cover.

[0021] In a preferred embodiment of the present invention, the cracking turntable further comprises a bottom plate with an opening at the top and a sealing at the bottom, wherein the bottom plate has a containing space intersecting with the top opening of the bottom plate, wherein the inner plate body and the outer plate body can be rotatably disposed in the containing space respectively, wherein a fixed shaft is fixedly disposed in the containing space, and the inner plate body is disposed around the outer periphery of the fixed shaft,

[0022] The liquid injection mechanism is arranged on the top of the disk cover;

[0023] Alternatively, the disc cover is in the shape of a ring, and is arranged on the top opening of the bottom plate and is arranged around the outer circumference of the fixed shaft, and the liquid injection mechanism is arranged on the top of the fixed shaft.

[0024] In a preferred embodiment of the present invention, the disk cover is provided with a plurality of first through holes spaced apart along its circumference, the distance between the first through holes and the central axis of the outer disk body is equal to the distance between the sample initial hole and the central axis of the outer disk body, and the angle between two adjacent first through holes in the circumference of the disk cover is equal to the angle between two adjacent sample initial holes in the circumference of the outer disk body;

[0025] The disk cover is provided with a plurality of second through holes spaced apart along its circumference, the distance between the second through holes and the central axis of the inner disk body is equal to the distance between the sample processing hole and the central axis of the inner disk body, and the angle between two adjacent second through holes in the circumference of the disk cover is equal to the angle between two adjacent sample processing holes in the circumference of the inner disk body;

[0026] The disk cover is provided with a plurality of hole position identification parts, and the plurality of hole position identification parts are used to mark the number and positions of the plurality of first through holes and / or the plurality of second through holes.

[0027] In a preferred embodiment of the present invention, the injection mechanism further includes a mechanical arm and a liquid storage container, the liquid outlet of the liquid storage container is connected to the injection tube through an infusion pipeline, and a pumping motor is provided on the infusion pipeline, and the pumping motor is used to pump the magnetic bead lysis solution stored in the liquid storage container to the injection tube;

[0028] The fixed end of the mechanical arm is connected to the lysis turntable, and the injection tube is arranged at the movable end of the mechanical arm to adjust the position of the injection tube.

[0029] In a preferred embodiment of the present invention, the mechanical arm comprises a mounting base, the liquid storage container is arranged on the mounting base, and a vertical pole is arranged on the mounting base, a lifting arm capable of vertical movement is arranged on the vertical pole, a horizontal arm is arranged on the top of the lifting arm, and the pumping motor and the injection pipe are both arranged on the horizontal arm;

[0030] The vertical pole is provided with a guide rail extending vertically, the lifting arm is slidably connected to the guide rail, the mounting base is provided with a driving motor, the driving shaft of the driving motor is vertically upward and connected to the bottom end of the vertically arranged lead screw, the cross arm is connected to the top end of the lifting arm, the bottom end of the lifting arm is provided with a connecting block, the connecting block has a threaded hole, and the connecting block is screwed to the lead screw through the threaded hole.

[0031] As described above, the characteristics and advantages of the lysis device for automated nucleic acid extraction of the present invention are:

[0032] During the working process, the sample tube can be placed into the sample initial hole vertically opposite to it through the sample loading station, and the sample tube is opened when it is rotated to the opening station (so as to inject magnetic bead lysis solution into the sample tube in the subsequent operation); then, the sample tube is transferred from the first sample loading station to the second sample loading station, so that the sample tube can rotate along the second direction with the inner disk body, and when the sample tube is rotated along the second direction to the liquid injection station, the magnetic bead lysis solution is injected into the opened sample tube through the liquid injection tube, and the sample tube is heated at least in the subsequent heating station to increase the lysis rate of the nucleic acid; then, the sample tube is transferred from the first sample output station to the second sample output station, so that the sample tube can rotate along the first direction with the outer disk body again When the sample tube rotates along the first direction to the capping and sample separation station, the cap of the sample tube can be removed to complete the lysis treatment of the sample in the sample tube. During the whole process, the sample tube can accurately find the corresponding station to complete the corresponding operation, which is faster and more accurate, and realizes the on-demand collection of samples to be lysed, thereby accelerating the lysis rate of nucleic acid extraction. Due to the setting of multiple porous positions (i.e., multiple sample initial holes and multiple sample processing holes), when applied to scenarios with huge sample quantities, the samples to be lysed will not be unable to be processed in time. In addition, the structure of the present invention is more streamlined, the volume is reduced, the cost is lower, and the simplicity of the structure further simplifies the operation process of nucleic acid extraction, improves the lysis rate, and has a better use effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] The following drawings are only intended to illustrate and explain the present invention, and are not intended to limit the scope of the present invention.

[0034] in:

[0035] Figure 1 : It is the operation flow chart of the magnetic bead purification method in the prior art.

[0036] Figure 2 : One of the stereoscopic views of the lysis device for automated nucleic acid extraction of the present invention.

[0037] Figure 3 : A top view of the internal structure of the lysis turntable in the lysis device for automated nucleic acid extraction of the present invention.

[0038] Figure 4 :for Figure 2 A partial enlarged view of position A in the middle.

[0039] Figure 5 :for Figure 2 A partial enlarged view of position B in the middle.

[0040] Figure 6 :for Figure 2 A partial enlarged view of the C position in the middle.

[0041] Figure 7 :for Figure 2 A partial enlarged view of the D position in the middle.

[0042] Figure 8 : It is a structural schematic diagram of the liquid injection mechanism in the lysis device for automated nucleic acid extraction of the present invention.

[0043] Fig. 9 : This is the second stereoscopic diagram of the lysis device for automated nucleic acid extraction of the present invention.

[0044] The accompanying drawings in the present invention are:

[0045] 1. Liquid injection mechanism; 101. Mounting base;

[0046] 102. driving motor; 103. liquid storage container;

[0047] 104, pole; 1041, guide rail;

[0048] 105. lifting arm; 1051. connecting block;

[0049] 106. Screw rod; 107. Cross arm;

[0050] 108. Pumping motor; 109. Liquid injection pipe;

[0051] 2. Cracking turntable; 201. Chassis;

[0052] 2011, inner plate; 2012, outer plate;

[0053] 2013, sample initial well; 2014, sample processing well;

[0054] 2015, sample tube holder; 202, tray cover;

[0055] 2021, first sample loading station; 2022, second sample loading station;

[0056] 2023, liquid filling station; 2024, cover opening station;

[0057] 2025, sample loading station; 2026, heating station;

[0058] 2027, capping and sample removal station; 2028, second sample removal station;

[0059] 2029, first sample output station; 2030, first through hole;

[0060] 2031, second through hole; 2032, hole position identification portion;

[0061] 203. Fixed axis; 3. Sample tube. DETAILED DESCRIPTION

[0062] In order to have a clearer understanding of the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described with reference to the accompanying drawings.

[0063] The present invention aims to provide a lysis device for automated nucleic acid extraction, which is easy to operate, has a fast lysis rate, is integrated, streamlined, and has a reduced cost. Figure 1 The sample to be tested is subjected to a nucleic acid lysis operation to release the nucleic acid.

[0064] The lysis device for automated nucleic acid extraction of the present invention can, on the one hand, realize on-demand collection of samples to be tested, thereby accelerating the lysis rate of nucleic acid extraction, and at the same time, when a huge amount of samples is used, there will be no situation where the samples to be tested cannot be processed in time; on the other hand, the structural design of the lysis device is simpler, the volume is reduced, and the cost is reduced. At the same time, due to its streamlined structure, the operation process is further simplified, and the lysis rate is effectively improved.

[0065] like Figures 2 to 9 As shown, the present invention provides a lysis device for automatic nucleic acid extraction, the lysis device for automatic nucleic acid extraction comprises: a lysis turntable 2, the lysis turntable 2 has an inner disk body 2011 and an outer disk body 2012 both in annular shape, the outer disk body 2012 is arranged around the outer circumference of the inner disk body 2011, and a plurality of sample initial holes 2013 for accommodating sample tubes 3 (the sample tubes 3 are pre-filled with samples to be lysed) are evenly arranged on the outer disk body 2012 and at intervals along the circumference of the outer disk body 2012, and the inner disk body 2011 is provided with a plurality of sample initial holes 2013 for accommodating sample tubes 3 (the sample tubes 3 are pre-filled with samples to be lysed). Multiple sample processing holes 2014 for accommodating sample tubes 3 are evenly arranged at intervals along the circumference of the inner disk body 2011. Along the radial direction of the lysis turntable 2, the multiple sample initial holes 2013 correspond to the multiple sample processing holes 2014 one by one, that is, the multiple sample initial holes 2013 and the corresponding sample processing holes 2014 are located on the same radial extension line of the lysis turntable 2. The outer disk body 2012 can rotate in a first direction, while the inner disk body 2011 can rotate in a second direction. The first direction and the second direction are opposite directions. The first direction can be Figure 2 The second direction can be counterclockwise. Figure 2 In the clockwise direction.

[0066] The lysis turntable 2 also includes a disk cover 202, which is located above the inner disk body 2011 and the outer disk body 2012, and covers a plurality of sample initial holes 2013 and a plurality of sample processing holes 2014. The disk cover 202 is provided with a second sample discharging station 2028, a capping and sample separation station 2027, a sample loading station 2025, a cover opening station 2024 and a first sample loading station 2021 in sequence along a first direction, and the second sample discharging station 2028 is provided at a first position. The distances between the central axis of the outer plate 2012 and the sample initial hole 2013 are equal to the distance between the sample initial hole 2013 and the central axis of the outer plate 2012. During the rotation of the outer plate 2012, any sample initial hole 2013 can be rotated to the second sample output station 2028, the capping and sample separation station 2027, the sample loading station 2025, the uncapping station 2024 and the first sample loading station 2021. , the opening station 2024 and the first sample introduction station 2021 are vertically opposite to each other; the second sample introduction station 2022, the injection station 2023, the heating station 2026 and the first sample output station 2029 are sequentially arranged on the disk cover 202 along the second direction, and the distances between the second sample introduction station 2022, the injection station 2023, the heating station 2026 and the first sample output station 2029 and the central axis of the inner disk body 2011 are all equal to the distance between the sample processing hole 2014 and the central axis of the inner disk body 2011. During the rotation of the inner disk body 2011, any sample processing hole 2014 can be rotated to a position vertically opposite to the second sample introduction station 2022, the injection station 2023, the heating station 2026 and the first sample output station 2029, so that different operations are performed on the vertically opposite sample tubes 3 at different stations, and finally the purpose of completing the lysis of the sample to be lysed in the sample tube 3 is achieved;

[0067] like Figure 2 , Figure 8 , Fig. 9 As shown, the lysis device for automated nucleic acid extraction also includes an injection mechanism 1, which includes an injection tube 109 for outputting magnetic bead lysis solution. The injection tube 109 is located above the lysis turntable 2 and vertically opposite to the injection station 2023.

[0068] In the present invention, the lysis turntable 2 has an inner disk body 2011 and an outer disk body 2012 that can rotate in opposite directions (that is, the outer disk body 2012 and the inner disk body 2011 can rotate in opposite first and second directions respectively), and the outer disk body 2012 and the inner disk body 2011 are respectively provided with a plurality of sample initial holes 2013 and a plurality of sample processing holes 2014 that can accommodate the sample tubes 3 at intervals and evenly along their respective circumferential directions; a disk cover 202 is covered above the plurality of sample initial holes 2013 and the plurality of sample processing holes 2014, and a second sample discharging station 2028, a capping and sample separation station 2027, a sample loading station 2025, a cover opening station 2024 and a first sample loading station 2021 are sequentially provided on the disk cover 202 along the first direction, and the second sample discharging station 2028, a capping and sample separation station 2027, a sample loading station 2025, a cover opening station 2024 and a first sample loading station 2021 are sequentially provided. The sample loading station 2028, the capping and sample separation station 2027, the loading station 2025, the cover opening station 2024 and the first sample loading station 2021 are all at equal radial distances from the sample initial hole 2013. The second sample loading station 2022, the liquid injection station 2023, the heating station 2026 and the first sample output station 2029 are sequentially arranged on the disk cover 202 along the second direction, and the second sample loading station 2022, the liquid injection station 2023, the heating station 2026 and the first sample output station 2029 are all at equal radial distances from the sample processing hole 2014. During the working process, the sample tube 3 can be placed into the sample initial hole 2013 vertically opposite to it through the loading station 2025, and the sample tube 3 can be placed when it is rotated to the cover opening station 2024. The cover is opened (so that the magnetic bead lysis solution can be injected into the sample tube 3 in the subsequent operation); thereafter, the sample tube 3 is transferred from the first sample injection station 2021 to the second sample injection station 2022, so that the sample tube 3 can rotate along the second direction with the inner disk body 2011. When the sample tube 3 rotates along the second direction to the liquid injection station 2023, the magnetic bead lysis solution is injected into the opened sample tube 3 through the liquid injection tube 109 of the liquid injection mechanism 1, and the sample tube 3 is heated at least in the subsequent heating station 2026 to increase the lysis rate of the nucleic acid; thereafter, the sample tube 3 is transferred from the first sample output station 2029 to the second sample output station 2028, so that the sample tube 3 can rotate along the first direction with the outer disk body 2012 again. When the sample tube 3 rotates along the second direction to the liquid injection station 2023, the magnetic bead lysis solution is injected into the opened sample tube 3 through the liquid injection tube 109 of the liquid injection mechanism 1, and the sample tube 3 is heated at least in the subsequent heating station 2026 to increase the lysis rate of the nucleic acid; When the first direction is rotated to the capping and sample removal station 2027, the cap of the sample tube 3 can be removed to complete the lysis treatment of the sample in the sample tube 3. During the whole process, the sample tube 3 can accurately find the corresponding station and complete the corresponding operation, which is faster and more accurate, and realizes the on-demand collection of samples to be lysed, thereby accelerating the lysis rate of nucleic acid extraction. Due to the setting of multiple porous positions (i.e., multiple sample initial holes 2013 and multiple sample processing holes 2014), when applied to scenarios with huge sample quantities, the samples to be lysed will not be unable to be processed in time. In addition, the structure of the present invention is more streamlined, the volume is reduced, the cost is lower, and the simplicity of the structure further simplifies the operation process of nucleic acid extraction, improves the lysis rate, and has a better use effect.

[0069] In the present invention, Figure 3 As shown, along the radial direction of the lysis turntable 2, the multiple sample initial holes 2013 correspond to the multiple sample processing holes 2014 one by one, that is, the multiple sample initial holes 2013 and the corresponding sample processing holes 2014 are located on the same radius extension line of the lysis turntable 2. Each sample processing hole 2014 on the inner disk body 2011 corresponds to a sample initial hole 2013 on the outer disk body 2012, and it is ensured that the angle of each rotation of the inner disk body 2011 is equal to the angle of each rotation of the outer disk body 2012, so that after each rotation of the inner disk body 2011 and the outer disk body 2012, the multiple sample processing holes 2014 are still located on the same radius extension line with the multiple sample initial holes 2013.

[0070] Further, such as Figures 4 to 7 As shown, a sample tube holder 2015 is respectively provided in the sample initial hole 2013 and the sample processing hole 2014. The sample tube holder 2015 is in the shape of a cylinder with an open top. The sample tube holder 2015 is adapted to the sample tube 3 so that the sample tube 3 can be inserted into the sample tube holder 2015 and ensure the stable placement of the sample tube 3.

[0071] Specifically, when the outer disk body 2012 rotates along the first direction, the sample tube 3 containing the sample to be lysed is placed from the loading station 2025 into any sample initial hole 2013 vertically opposite thereto, and the sample tube 3 is opened when the sample tube 3 rotates along with the outer disk body 2012 to be vertically opposite to the opening station 2024; when the sample tube 3 rotates along with the outer disk body 2012 along the first direction to be vertically opposite to the first injection station 2021, the sample tube 3 is transferred from the sample initial hole 2013 to any sample processing hole 2014 vertically opposite to the second injection station 2022.

[0072] Specifically, when the sample tube 3 rotates along the second direction with the inner disk body 2011 to be vertically opposite to the injection station 2023, the magnetic bead lysis solution is injected into the sample tube 3 through the injection tube 109, and the sample tube 3 is heated at least when the sample tube 3 rotates along the inner disk body 2011 to a position vertically opposite to the heating station 2026; when the sample tube 3 rotates along the second direction with the inner disk body 2011 to be vertically opposite to the first sample output station 2029, the sample tube 3 is transferred from the first sample output station 2029 to any sample initial hole 2013 vertically opposite to the second sample output station 2028.

[0073] Specifically, when the sample tube 3 rotates along the first direction with the outer disk 2012 to be vertically opposite to the capping and sample separation station 2027, the capping and sample removal process is performed on the sample tube 3.

[0074] In an optional embodiment of the present invention, a heating element is provided at the heating station 2026, so that the sample tube 3 passing through the heating station 2026 can be heated to accelerate the lysis of the sample to be lysed in the sample tube 3, quickly release the nucleic acid, and improve the lysis efficiency. Of course, a heating element can also be provided at any position from the second sample injection station 2022 to the first sample output station 2029, so that after the lysis solution is injected, the sample tube 3 can be heated to a temperature range suitable for lysis. The heating element can be, but is not limited to, an electric heating element (such as a heating resistor).

[0075] In an optional embodiment of the present invention, Figure 2 , Fig. 9 As shown, the second sample discharging station 2028, the capping and sample separation station 2027, the sample loading station 2025, the lid opening station 2024, the first sample injection station 2021, the second sample injection station 2022, the liquid injection station 2023, the heating station 2026 and the first sample discharging station 2029 are all through holes opened on the disk cover 202, and the second sample discharging station 2028, the capping and sample separation station 2027, the sample loading station 2025, the lid opening station 2024, the first sample injection station 2021, the second sample injection station 2022, the liquid injection station 2023, the heating station 2026 and the first sample discharging station 2029 are all located within the same semicircle of the disk cover 202.

[0076] Further, if Figure 4 , Figure 6As shown, the first sample loading station 2021 and the second sample loading station 2022 are located on the extension line of the same radius of the disk cover 202, and the first sample loading station 2021 is connected to the second sample loading station 2022, so as to facilitate the transfer of the sample tube 3 from the first sample loading station 2021 to the second sample loading station 2022. The second sample output station 2028 and the first sample output station 2029 are located on the extension line of the same radius of the disk cover 202, and the second sample output station 2028 is connected to the first sample output station 2029, so as to facilitate the transfer of the sample tube 3 from the first sample output station 2029 to the second sample output station 2028. The positions of the first sample injection station 2021 and the second sample injection station 2022 on the disk cover 202 are 180° apart from the positions of the first sample output station 2029 and the second sample output station 2028 on the disk cover 202. When the sample tube 3 in the sample initial hole 2013 vertically opposite to the first sample injection station 2021 is transferred to the sample processing hole 2014 vertically opposite to the second sample injection station 2022, it can be determined that there is no sample tube 3 in the sample initial hole 2013 vertically opposite to the first sample injection station 2021. Subsequently, after the inner disk body 2011 rotates 180°, the sample tube 3 is transferred to the sample processing hole 2014 vertically opposite to the second sample injection station 2022. The sample tube 3 in the vertically opposite sample processing hole 2014 is rotated to a position vertically opposite to the first sample output station 2029, so that the sample initial hole 2013 without the sample tube 3 is determined to rotate 180° with the outer disk body 2012 to reach a position vertically opposite to the second sample output station 2028. Therefore, the sample tube 3 can be transferred from the sample processing hole 2014 vertically opposite to the first sample output station 2029 to the sample initial hole 2013 vertically opposite to the second sample output station 2028, avoiding the situation that the sample tube 3 exists in the sample initial hole 2013 vertically opposite to the second sample output station 2028 and cannot be transferred.

[0077] Further, such as Figure 5 As shown, the sample loading station 2025 and the heating station 2026 are located on the extension line of the same radius of the disk cover 202 , and the sample loading station 2025 is connected to the heating station 2026 .

[0078] In an optional embodiment of the present invention, Figure 2 , Fig. 9As shown, a plurality of first through holes 2030 are spaced and evenly distributed along the circumference of the disk cover 202, the distance between the first through holes 2030 and the central axis of the outer disk body 2012 is equal to the distance between the sample initial hole 2013 and the central axis of the outer disk body 2012, and the angle between two adjacent first through holes 2030 in the circumference of the disk cover 202 is equal to the angle between two adjacent sample initial holes 2013 in the circumference of the outer disk body 2012. Therefore, after each rotation of the outer disk body 2012, the plurality of first through holes 2030 are still located at positions corresponding to the plurality of sample initial holes 2013 in the vertical direction, and the staff can obtain the rotation position of the sample tube 3 through the first through holes 2030, thereby obtaining the progress of the lysis.

[0079] Further, such as Figure 2 , Fig. 9 As shown, a plurality of second through holes 2031 are spaced apart and evenly distributed along the circumference of the disk cover 202, the distance between the second through holes 2031 and the central axis of the inner disk body 2011 is equal to the distance between the sample processing hole 2014 and the central axis of the inner disk body 2011, and the angle between two adjacent second through holes 2031 in the circumference of the disk cover 202 is equal to the angle between two adjacent sample processing holes 2014 in the circumference of the inner disk body 2011. Therefore, after each rotation of the inner disk body 2011, the plurality of second through holes 2031 are still located at positions corresponding one by one to the plurality of sample processing holes 2014 in the vertical direction, and have the same function and effect as the setting of the first through holes 2030, which will not be elaborated here.

[0080] Further, such as Fig. 9 As shown, a plurality of hole position identification portions 2032 may be provided on the top surface of the disk cover 202, and the plurality of hole position identification portions 2032 correspond one-to-one to the plurality of first through holes 2030, and the plurality of hole position identification portions 2032 also correspond one-to-one to the plurality of second through holes 2031. The plurality of hole position identification portions 2032 may be used to mark the number and positions of the plurality of first through holes 2030 and / or the plurality of second through holes 2031, and the number of sample tubes 3 that can be accommodated by the lysis device of the present invention may be directly known through the hole position identification portions 2032, thereby knowing the one-time sample processing volume.

[0081] In an optional embodiment of the present invention, Figure 2 , Figure 3 , Fig. 9As shown, the lysis turntable 2 also includes a cylindrical bottom plate 201 with an opening at the top and a sealed bottom, the bottom plate 201 has a containing space intersecting with the top opening of the bottom plate 201, the inner plate body 2011 and the outer plate body 2012 can be rotatably arranged in the containing space respectively, a fixed shaft 203 is also fixed in the containing space, the inner plate body 2011 can be rotatably arranged around the outer periphery of the fixed shaft 203, the plate cover 202 is annular, the plate cover 202 is covered at the top opening of the bottom plate 201 and is arranged around the outer periphery of the fixed shaft 203, and the liquid injection mechanism 1 is arranged on the top of the fixed shaft 203, thereby providing an installation position for the liquid injection mechanism 1. Of course, if the plate cover 202 is a disc, and the fixed shaft 203 is covered by the plate cover 202 in the containing space, the liquid injection mechanism 1 can be arranged on the top of the plate cover 202.

[0082] In the present invention, the rotation of the inner disk 2011 and the outer disk 2012 can be directly driven by a motor, or the motor can provide driving force, and the transmission is performed through a transmission structure such as a screw transmission or a gear transmission, so as to drive the inner disk 2011 and the outer disk 2012 to rotate. However, it is necessary to ensure that the rotation angle of the inner disk 2011 and the outer disk 2012 is the same for each rotation step.

[0083] In a specific embodiment of the present invention, Figure 2 , Figure 8 , Fig. 9 As shown, the injection mechanism 1 also includes a liquid storage container 103, the liquid outlet of the liquid storage container 103 is connected to the injection tube 109 through an infusion pipeline, and a pumping motor 108 is provided on the infusion pipeline. The pumping motor 108 is used to pump the magnetic bead lysate stored in the liquid storage container 103 to the injection tube 109, so as to inject the magnetic bead lysate into the corresponding sample tube 3. By controlling the working state of the pumping motor 108, the amount of the magnetic bead lysate injected into the sample tube 3 can be controlled to improve the accuracy of the injected magnetic bead lysate, and ensure that the nucleic acid can be quickly and fully released. Among them, the magnetic bead lysate is a mixed solution containing magnetic beads and a lysing reagent required for sample lysing, and the specific type of lysing reagent is a prior art, and those skilled in the art can select it according to the sample that actually needs to be lysed, and it is not limited here.

[0084] In a specific embodiment of the present invention, Figure 2 , Figure 8 , Fig. 9As shown, the injection mechanism 1 also includes a robotic arm having a fixed end and a movable end, the fixed end of the robotic arm being connected to the lysis turntable 2, and the injection tube 109 being arranged at the movable end of the robotic arm, and the robotic arm can drive the injection tube 109 to adjust its position, thereby ensuring that the bottom injection port of the injection tube 109 can be located at a position vertically opposite to the injection station 2023, thereby ensuring that the magnetic bead lysis solution can be accurately injected into the sample tube 3 vertically opposite to the injection station 2023.

[0085] Specifically, Figure 2 , Figure 8 , Fig. 9 As shown, the robotic arm includes a mounting base 101, which is fixedly arranged on the top of the lysis turntable 2, and a liquid storage container 103 is arranged on the mounting base 101, and a vertically extending vertical pole 104 is arranged on the mounting base 101, and a vertically movable lifting arm 105 is arranged on the vertical pole 104, and a horizontal arm 107 extending in the horizontal direction is arranged on the top of the lifting arm 105, and a pumping motor 108 and an injection pipe 109 are both arranged on the horizontal arm 107. By adjusting the lifting height of the lifting arm 105 on the vertical pole 104, the height of the horizontal arm 107 can be adjusted to achieve the purpose of adjusting the height of the injection pipe 109.

[0086] Further, such as Figure 2 , Figure 8 , Fig. 9 As shown, a guide rail 1041 extending vertically is provided on the vertical pole 104, and the lifting arm 105 can be slidably connected to the guide rail 1041. A driving motor 102 is provided on the mounting base 101. The driving shaft of the driving motor 102 is vertically upward and connected to the bottom end of the vertically arranged lead screw 106. The cross arm 107 is connected to the top of the lifting arm 105. A connecting block 1051 is provided at the bottom end of the lifting arm 105 (the lifting arm 105 and the connecting block 1051 can be integrally formed in an "L" shape), and a threaded hole is provided on the connecting block 1051, and the connecting block 1051 is screwed to the lead screw 106 through the threaded hole. During the actual working process, the screw rod 106 is driven to rotate by the driving motor 102. Since the lifting arm 105 is screwed to the screw rod 106 through the connecting block 1051, and the lifting arm 105 is limited in the circumferential direction, when the screw rod 106 rotates, the lifting arm 105 can only rise or fall along the screw rod 106 according to the rotation direction of the screw rod 106, so as to achieve the purpose of adjusting the height of the injection tube 109.

[0087] The working process of the lysis device for automated nucleic acid extraction of the present invention is as follows:

[0088] The sample tube 3 containing the sample to be lysed is placed in the sample initial hole 2013 vertically opposite to the sample loading station 2025 at the sample loading station 2025, and then the inner disk body 2011 rotates clockwise, and the outer disk body 2012 rotates counterclockwise synchronously. When the outer disk body 2012 rotates to the next sample initial hole 2013 vertically opposite to the sample loading station 2025, the next sample tube 3 containing the sample to be lysed can be placed. As the outer disk body 2012 rotates, the placement of the sample tube 3 is similar.

[0089] After the sample tube 3 containing the sample to be lysed is placed in the sample initial hole 2013 vertically opposite to the sample loading station 2025, the inner disk 2011 and the outer disk 2012 continue to rotate in opposite directions respectively. When the sample tube 3 rotates to be vertically opposite to the cover opening station 2024, the inner disk 2011 and the outer disk 2012 stop rotating at the same time, and the cover on the sample tube 3 is opened manually by a robot or a staff member;

[0090] After the cover on the sample tube 3 is opened, the inner disk 2011 and the outer disk 2012 continue to rotate in opposite directions. When the sample tube 3 rotates to the first sample injection station 2021, the inner disk 2011 and the outer disk 2012 stop rotating at the same time again, and the sample tube 3 is transferred from the sample initial hole 2013 vertically opposite to the first sample injection station 2021 to the sample processing hole 2014 vertically opposite to the second sample injection station 2022 by a manipulator or manually by a staff member;

[0091] Afterwards, the inner disk body 2011 and the outer disk body 2012 continue to rotate in opposite directions respectively (when the sample processing hole 2014 where the sample tube 3 is located rotates clockwise with the inner disk body 2011 to a position vertically opposite to the first sample output station 2029, the sample initial hole 2013 corresponding to the sample processing hole 2014 just rotates counterclockwise with the outer disk body 2012 to a position vertically opposite to the second sample output station 2028). When the sample tube 3 rotates to a position vertically opposite to the injection station 2023, the drive motor 102 and the pumping motor 108 are started at the same time to inject the magnetic bead lysis solution into the opened sample tube 3;

[0092] Afterwards, the inner disk 2011 and the outer disk 2012 continue to rotate in opposite directions. When the sample tube 3 rotates to a position vertically aligned with the heating station 2026, the sample to be lysed and the magnetic bead lysing solution in the sample tube 3 are heated, thereby promoting the lysis of the sample to be lysed.

[0093] Afterwards, the inner disk 2011 and the outer disk 2012 continue to rotate in opposite directions respectively. When the sample tube 3 rotates to a position vertically opposite to the first sample output station 2029, the sample tube 3 is no longer heated. At the same time, the sample tube 3 is transferred from the sample processing hole 2014 vertically opposite to the first sample output station 2029 to the sample initial hole 2013 vertically opposite to the second sample output station 2028 by a manipulator or a staff member manually.

[0094] Afterwards, the inner disk body 2011 and the outer disk body 2012 continue to rotate in opposite directions respectively. When the sample tube 3 rotates to a position vertically opposite to the capping and sample separation station 2027, the lid is manually covered on the top opening of the sample tube 3 by a robot or a staff member to complete the lysis operation of the sample to be lysed, and the sample tube 3 can be removed from the lysis turntable 2.

[0095] The characteristics and advantages of the lysis device for automated nucleic acid extraction of the present invention are:

[0096] 1. The lysis device for automated nucleic acid extraction can not only realize the on-demand collection of samples, but also accelerate the lysis rate of nucleic acid extraction. When applied to scenarios with a large number of samples, the samples to be tested will not be unable to be processed in time. The entire device is in a state of being able to accept samples at any time, and there will be no need to wait for full load.

[0097] Second, the lysis device for automated nucleic acid extraction has a more streamlined structure, reduced volume, and lower cost. In addition, its streamlined structure further simplifies the operation process of nucleic acid extraction, improves the lysis rate, and has a better use effect.

[0098] 3. The lysis device for automated nucleic acid extraction can automatically add magnetic bead lysis solution to the sample tube 3 vertically aligned therewith through the liquid injection mechanism 1, thereby greatly improving the degree of automation as well as the reliability and accuracy of operation.

[0099] 4. In the lysis device for automated nucleic acid extraction, after adding the magnetic bead lysis solution to the sample tube 3, the sample tube 3 can be heated and the temperature can be stabilized within a range suitable for nucleic acid lysis, thereby increasing the nucleic acid lysis rate and improving the lysis effect.

[0100] The above description is only an illustrative embodiment of the present invention and is not intended to limit the scope of the present invention. Any equivalent changes and modifications made by any person skilled in the art without departing from the concept and principle of the present invention shall fall within the scope of protection of the present invention.

Claims

1. A lysis device for automated nucleic acid extraction, characterized in that: The lysis device for automated nucleic acid extraction comprises: A lysis turntable, the lysis turntable comprising an inner disk body and an outer disk body, the outer disk body being arranged around the outer circumference of the inner disk body, the outer disk body being provided with a plurality of sample initial holes for accommodating sample tubes at intervals along its circumference, the inner disk body being provided with a plurality of sample processing holes for accommodating the sample tubes at intervals along its circumference, along the radial direction of the lysis turntable, the plurality of sample initial holes correspond to the plurality of sample processing holes one by one, the outer disk body and the inner disk body being respectively rotatable in a first direction and a second direction, the first direction being opposite to the second direction; The lysis turntable further comprises a disk cover, the disk cover is located above the inner disk body and the outer disk body, and the disk cover covers the plurality of sample initial holes and the plurality of sample processing holes, and the disk cover is provided with a second sample discharging station, a capping and sample separation station, a sample loading station, a cover opening station and a first sample loading station in sequence along the first direction, and the distances between the second sample discharging station, the capping and sample separation station, the sample loading station, the cover opening station and the first sample loading station and the central axis of the outer disk body are all equal to the distance between the sample initial holes and the central axis of the outer disk body; the disk cover is provided with a second sample loading station, a liquid injection station, a heating station and a first sample discharging station in sequence along the second direction, and the distances between the second sample loading station, the liquid injection station, the heating station and the first sample discharging station and the central axis of the inner disk body are all equal to the distance between the sample processing holes and the central axis of the inner disk body; The liquid injection mechanism comprises a liquid injection tube for outputting magnetic bead lysis solution, and the liquid injection tube is located above the lysis turntable and vertically opposite to the liquid injection station.

2. The lysis device for automated nucleic acid extraction according to claim 1, characterized in that: When the outer disk body rotates along the first direction, the sample tube containing the sample to be lysed is placed from the sample loading station into any of the sample initial holes vertically opposite thereto, and the sample tube is uncapped when the sample tube rotates with the outer disk body to be vertically opposite to the uncapping station; When the sample tube rotates along the first direction with the outer disk to be vertically opposite to the first sample injection station, the sample tube is transferred from the sample initial hole to any of the sample processing holes vertically opposite to the second sample injection station.

3. The lysis device for automated nucleic acid extraction according to claim 2, characterized in that: When the sample tube rotates along the second direction with the inner disk body to be vertically opposite to the injection station, the magnetic bead lysis solution is injected into the sample tube through the injection tube, and the sample tube is heated at least when the sample tube rotates along the inner disk body to a position vertically opposite to the heating station; When the sample tube rotates along the second direction with the inner disk to be vertically opposite to the first sample output station, the sample tube is transferred from the first sample output station to any sample initial hole vertically opposite to the second sample output station.

4. The lysis device for automated nucleic acid extraction according to claim 3, characterized in that: When the sample tube rotates along the first direction with the outer disk to be vertically opposite to the capping and sample separation station, the capping and sample removal process is performed on the sample tube.

5. The lysis device for automated nucleic acid extraction according to any one of claims 1 to 4, characterized in that: The second sample discharging station, the capping and sample separation station, the sample loading station, the lid opening station, the first sample injection station, the second sample injection station, the liquid injection station, the heating station and the first sample discharging station are all through holes opened on the disk cover and are all located within the same half circumference of the disk cover.

6. The lysis device for automated nucleic acid extraction according to claim 5, characterized in that: The first sample introduction station and the second sample introduction station are located on an extension line of the same radius of the disk cover; And / or, the loading station and the heating station are located on an extension line of the same radius of the plate cover; And / or, the second sample discharging station and the first sample discharging station are located on an extension line of the same radius of the disk cover.

7. The lysis device for automated nucleic acid extraction according to claim 1, characterized in that: The cracking turntable further comprises a bottom plate with an opening at the top and a sealing at the bottom, wherein the bottom plate has a containing space intersecting with the top opening of the bottom plate, wherein the inner plate body and the outer plate body can be rotatably arranged in the containing space respectively, wherein a fixed shaft is fixedly arranged in the containing space, and the inner plate body is arranged around the outer periphery of the fixed shaft, The liquid injection mechanism is arranged on the top of the disk cover; Alternatively, the disc cover is in the shape of a ring, and is arranged on the top opening of the bottom plate and is arranged around the outer circumference of the fixed shaft, and the liquid injection mechanism is arranged on the top of the fixed shaft.

8. The lysis device for automated nucleic acid extraction according to claim 7, characterized in that: The disk cover is provided with a plurality of first through holes spaced apart along its circumference, the distance between the first through holes and the central axis of the outer disk body is equal to the distance between the sample initial hole and the central axis of the outer disk body, and the angle between two adjacent first through holes in the circumference of the disk cover is equal to the angle between two adjacent sample initial holes in the circumference of the outer disk body; The disk cover is provided with a plurality of second through holes spaced apart along its circumference, the distance between the second through holes and the central axis of the inner disk body is equal to the distance between the sample processing hole and the central axis of the inner disk body, and the angle between two adjacent second through holes in the circumference of the disk cover is equal to the angle between two adjacent sample processing holes in the circumference of the inner disk body; The disk cover is provided with a plurality of hole position identification parts, and the plurality of hole position identification parts are used to mark the number and positions of the plurality of first through holes and / or the plurality of second through holes.

9. The lysis device for automated nucleic acid extraction according to claim 1, characterized in that: The injection mechanism also includes a mechanical arm and a liquid storage container, the liquid outlet of the liquid storage container is connected to the injection tube through an infusion pipeline, and a pumping motor is provided on the infusion pipeline, and the pumping motor is used to pump the magnetic bead lysate stored in the liquid storage container to the injection tube; The fixed end of the mechanical arm is connected to the lysis turntable, and the injection tube is arranged at the movable end of the mechanical arm to adjust the position of the injection tube.

10. The lysis device for automated nucleic acid extraction according to claim 9, characterized in that: The mechanical arm comprises a mounting base, the liquid storage container is arranged on the mounting base, and a vertical pole is arranged on the mounting base, a lifting arm capable of vertical movement is arranged on the vertical pole, a horizontal arm is arranged on the top of the lifting arm, and the pumping motor and the liquid injection pipe are both arranged on the horizontal arm; The vertical pole is provided with a guide rail extending vertically, the lifting arm is slidably connected to the guide rail, the mounting base is provided with a driving motor, the driving shaft of the driving motor is vertically upward and connected to the bottom end of the vertically arranged lead screw, the cross arm is connected to the top end of the lifting arm, the bottom end of the lifting arm is provided with a connecting block, the connecting block has a threaded hole, and the connecting block is screwed to the lead screw through the threaded hole.

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