Preparation method of soil remediation agent containing earthworms

Abstract

The invention provides a preparation method of a soil remediation agent containing earthworms, and belongs to the technical field of soil remediation. The soil remediation agent containing the earthworms is obtained through the following steps that fruit, vegetable and vegetable leaves and livestock and poultry manure are collected and mixed, and then a fermentation substrate is obtained; placing the earthworms in the fermentation substrate for culturing to obtain mature earthworms with reproductive girdles; the method comprises the following steps: performing segmented dissection on mature earthworms to obtain earthworm foreintestines; crushing and fermenting corn straws to obtain a nutrient medium; and mixing the earthworm foreintestines, a microbial agent and a nutrient medium to obtain the soil remediation agent containing the earthworms. The soil remediation agent can increase the number of microorganisms in soil, promote generation of drug-resistant flora and remedy damaged soil; meanwhile, the soil remediation agent is prepared from the raw materials such as the straw and the earthworms which do not cause secondary damage to the soil, the soil remediation agent is environmentally friendly, the preparation method of the soil remediation agent is simple and easy to implement, and the soil can be quickly and efficiently remedied.

Description

Technical Field

[0001] The present invention relates to the technical field of soil remediation, and in particular to a method for preparing a soil remediation agent containing earthworms. Background Art

[0002] Soil pollution is one of the major environmental problems facing the world today.

[0003] Soil pollution poses a serious threat to the sustainable use of land resources, the ecological safety of agricultural products, human health and social stability. Contaminated soil can lead to reduced crop yields and even cause excessive pollutants in agricultural products, which in turn endangers human health. In addition, with the acceleration of economic development and urbanization, site pollution caused by industrial and mining enterprises is also very serious. If these contaminated sites are not properly handled, they will become chemical time bombs. Once a large-scale outbreak occurs, it will have an immeasurable impact on the country's sustainable development. Therefore, it is of great significance to attach great importance to the prevention of soil pollution and the remediation of contaminated soil, and take effective measures to control it.

[0004] At present, soil remediation can be carried out by chemical remediation, physical remediation and biological remediation. Although physical remediation is highly effective, the cost is too high, and chemical remediation may introduce new pollutants. Biological remediation can repair soil efficiently and environmentally friendly, but the repair cycle is too long. Therefore, it is of great practical significance to provide a soil remediation agent that can quickly and efficiently repair soil without causing secondary damage to the soil. Summary of the invention

[0005] The object of the present invention is to provide a method for preparing a soil remediation agent containing earthworms. The soil remediation agent of the present invention can increase the number of microorganisms in the soil, promote the generation of drug-resistant bacteria, and repair damaged soil. At the same time, the present invention uses raw materials such as straw and earthworms that will not cause secondary damage to the soil to prepare the soil remediation agent, which is green and environmentally friendly. The preparation method of the soil remediation agent of the present invention is simple and easy, and can quickly and efficiently repair the soil.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a method for preparing a soil remediation agent containing earthworms, comprising the following steps:

[0008] Collecting fruit and vegetable leaves and livestock and poultry excrement, and first mixing the two to obtain a fermentation substrate;

[0009] placing earthworms in the fermentation substrate for culturing to obtain mature earthworms with reproductive annuli;

[0010] Dissecting the mature earthworms into sections to obtain the earthworm foregut, which is then refrigerated for later use;

[0011] The corn stalks are crushed and fermented to obtain a nutrient matrix;

[0012] The earthworm foregut and the microbial agent are mixed for the second time, and after mixing, they are placed in the nutrient matrix for the third mixing to obtain the soil remediation agent containing earthworms.

[0013] Furthermore, the mass ratio of fruit and vegetable leaves to livestock and poultry manure in the first mixture is 1-5:10-15.

[0014] Furthermore, the temperature of the first mixing is 20-23° C., the time is 2-5 days, and the humidity is 65-75%.

[0015] Furthermore, the mass ratio of the earthworms to the fermentation substrate is 1-5:100; the culture conditions are: temperature of 15-20° C., humidity of 70-80%, and time of 65-75 days.

[0016] Furthermore, the mass ratio of the earthworms to the corn stalks is 1 to 5:20.

[0017] Furthermore, the particle size of the corn stalks is 60-65 meshes, and the fermentation is performed by mixing the corn stalks with a glucose solution.

[0018] Furthermore, the mass fraction of the glucose solution is 20%, and the usage ratio of the corn stalks to the glucose solution is 1-2 kg:40-50 mL.

[0019] Furthermore, the fermentation conditions are: temperature of 35-45°C and time of 25-30 days.

[0020] Furthermore, the microbial agent includes 5-10 parts of Bacillus subtilis, 5-10 parts of actinomycetes and 3-5 parts of yeast, and the mass ratio of earthworm foregut to microbial agent in the second mixture is 10-15:2-3.

[0021] Furthermore, the mass ratio of earthworm foregut to nutrient matrix in the third mixture is 1 to 3:10; the temperature of the third mixture is 20 to 25° C., and the time is 1 to 2 days.

[0022] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0023] (1) The present invention uses earthworm foregut as an important raw material for soil repair, which can promote the generation of antibiotic-degrading bacteria, drug-resistant genes and antagonistic bacteria in the soil environment, promote the removal of soil antibiotics and high-risk drug-resistant genes, maintain the original ecological structure of indigenous flora, eliminate the selection pressure of drug-resistant bacteria, and reduce the risk of secondary transmission of soil drug-resistant genes.

[0024] (2) The present invention also uses microbial agents together with earthworm foregut to repair the soil. The microbial agents can increase the nutrition of soil microorganisms, increase the number of soil microorganisms, promote the reduction of carbon emissions, protect the environment, and repair the soil.

[0025] (3) The soil remediation agents of the present invention are prepared from raw materials such as straw, microbial agents, and earthworm foregut that will not cause secondary pollution to the soil. Compared with soil remediation using chemical materials, there is no potential environmental risk. In addition, the method of the present invention is simple and easy to implement, and can quickly and efficiently remediate the soil. DETAILED DESCRIPTION

[0026] The present invention provides a method for preparing a soil remediation agent containing earthworms, comprising the following steps:

[0027] Collecting fruit and vegetable leaves and livestock and poultry excrement, and first mixing the two to obtain a fermentation substrate;

[0028] placing earthworms in the fermentation substrate for culturing to obtain mature earthworms with reproductive annuli;

[0029] Dissecting the mature earthworms into sections to obtain the earthworm foregut, which is then refrigerated for later use;

[0030] The corn stalks are crushed and fermented to obtain a nutrient matrix;

[0031] The earthworm foregut and the microbial agent are mixed for the second time, and after mixing, they are placed in the nutrient matrix for the third mixing to obtain the soil remediation agent containing earthworms.

[0032] First, fruit and vegetable leaves and livestock and poultry excrement are collected and mixed to obtain a fermentation matrix.

[0033] The present invention preferably air-dries the collected fruit and vegetable leaves, filters the collected livestock and poultry feces, keeps the water content at 80-85%, and then mixes the two.

[0034] In the present invention, the mass ratio of fruit and vegetable leaves to livestock and poultry excrement in the first mixture is preferably 1-5:10-15, and more preferably 1-2:10.

[0035] In the present invention, the temperature of the first mixing is preferably 20-23° C., more preferably 20° C.; the time is preferably 2-5 days, more preferably 2-3 days; the humidity is preferably 65-75%, more preferably 65-70%.

[0036] The earthworms are placed in the fermentation medium for cultivation to obtain mature earthworms with reproductive annuli.

[0037] In the present invention, Eisenia fetida is preferably used for culture.

[0038] In the present invention, the mass ratio of the earthworms to the fermentation substrate is preferably 1-5:100; more preferably 1-2:100; the culture conditions are preferably: temperature of 15-20°C, humidity of 70-80%, and time of 65-75 days; more preferably temperature of 15-18°C, humidity of 70-75%, and time of 65-70 days.

[0039] The mature earthworms are dissected into sections to obtain the earthworm foregut, which is then refrigerated for later use.

[0040] The refrigeration of the present invention is preferably carried out at a temperature of 0 to 4° C. The obtained earthworm foregut is refrigerated to facilitate the subsequent use thereof in preparing a soil remediation agent.

[0041] The corn stalks are crushed and fermented to obtain a nutrient matrix.

[0042] The present invention uses corn stalks as one of the raw materials of the soil remediation agent. Its porous structure and high specific surface area make it have good adsorption performance, which can adsorb heavy metal ions and organic pollutants in the soil and reduce the harm of these pollutants to the soil ecosystem; at the same time, the addition of corn stalks can also improve the air permeability and water retention of the soil, promote the reproduction and activity of soil microorganisms, and thus improve the overall structure of the soil

[0043] In the present invention, the mass ratio of the earthworms to the corn stalks is preferably 1 to 5:20, and more preferably 1 to 2:20.

[0044] In the present invention, the particle size of the corn stalks is preferably 60-65 meshes, and the fermentation is preferably performed by mixing the corn stalks with a glucose solution.

[0045] Furthermore, the mass fraction of the glucose solution of the present invention is preferably 20%, and the usage ratio of the corn straw to the glucose solution is preferably 1-2kg:40-50mL, and further preferably 1-1.5kg:40-45mL.

[0046] In the present invention, the fermentation conditions are preferably: a temperature of 35-45° C., and a time of 25-30 days, and more preferably: a temperature of 35-40° C., and a time of 25 days.

[0047] The earthworm foregut and the microbial agent are mixed for the second time, and after mixing, they are placed in the nutrient matrix for the third mixing to obtain the soil remediation agent containing earthworms.

[0048] The present invention uses a mixture of microbial agents and earthworm foregut to repair the soil. The earthworm foregut can increase the number of microorganisms in the soil, promote the formation of drug-resistant bacteria, and repair damaged soil. The microbial agent can decompose organic matter in the soil and convert it into inorganic nutrients that can be directly absorbed and utilized by plants, such as nitrogen, phosphorus, potassium, etc. This process not only improves the fertility of the soil, but also provides necessary nutritional support for the growth of crops. Through the decomposition of microorganisms, organic matter in the soil can be recycled, which helps to maintain the balance and stability of the soil ecosystem.

[0049] In the present invention, the microbial agent preferably includes 5-10 parts of Bacillus subtilis, 5-10 parts of actinomycetes and 3-5 parts of yeast, and is further preferably 5-7 parts of Bacillus subtilis, 5-7 parts of actinomycetes and 3-4 parts of yeast; the mass ratio of earthworm foregut to microbial agent in the second mixture is preferably 10-15:2-3, and is further preferably 10-12:2-3.

[0050] In the present invention, the mass ratio of earthworm foregut to nutrient matrix in the third mixture is preferably 1-3:10, more preferably 1-2:10; the temperature of the third mixture is preferably 20-25°C, more preferably 23°C, and the time is preferably 1-2 days.

[0051] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0052] Example 1

[0053] (1) Collecting 1 kg of fruit and vegetable leaves and air-drying them; collecting 10 kg of livestock and poultry feces and filtering them to obtain livestock and poultry feces with a moisture content of 80-85%, and then stirring and mixing the livestock and poultry feces with fruit and vegetable leaves at a temperature of 20° C. and a humidity of 65%, and then standing for 2 days after mixing evenly to obtain a fermentation substrate;

[0054] (2) placing 100 g of Eisenia fetida in 10 kg of fermentation medium for culturing at a temperature of 15° C. and a humidity of 70% for 65 days, and obtaining mature Eisenia fetida with reproductive annuli after the culturing;

[0055] (3) dissecting the mature earthworm obtained by culture into segments to obtain the foregut of the mature earthworm, and refrigerating the foregut at 0 to 4° C. for later use;

[0056] (4) 2 kg of corn stalks were crushed, and the obtained particles were sieved to obtain corn stalk particles with a mesh size of 60 to 65, which were mixed with 50 mL of a 20% glucose solution by mass, and fermented at 35° C. for 25 days to obtain a nutrient matrix;

[0057] (5) 5 parts of Bacillus subtilis, 5 parts of actinomycetes and 3 parts of yeast are mixed to obtain a microbial agent, 10 g of the above-mentioned mature refrigerated earthworm foregut is mixed with 2 g of the microbial agent, and then the mixture is mixed with 100 g of the above-mentioned nutrient matrix at 20° C. for 1 day to obtain the soil remediation agent containing earthworms.

[0058] Example 2

[0059] (1) Collecting 1 kg of fruit and vegetable leaves and air-drying them; collecting 5 kg of livestock and poultry feces and filtering them to obtain livestock and poultry feces with a moisture content of 80-85%, and then stirring and mixing the livestock and poultry feces with fruit and vegetable leaves at a temperature of 23° C. and a humidity of 70%, and then standing for 3 days after mixing evenly to obtain a fermentation substrate;

[0060] (2) 50 g of Eisenia fetida was placed in 2.5 kg of fermentation medium for culturing at a temperature of 18° C. and a humidity of 75% for 70 days. After the culturing, mature Eisenia fetida with reproductive annuli were obtained.

[0061] (3) dissecting the mature earthworm obtained by culture into segments to obtain the foregut of the mature earthworm, and refrigerating the foregut at 0 to 4° C. for later use;

[0062] (4) 0.5 kg of corn stalks were crushed, and the obtained particles were sieved to obtain corn stalk particles with a mesh size of 60 to 65, which were mixed with 20 mL of a 20% glucose solution by mass, and fermented at 40° C. for 25 days to obtain a nutrient matrix;

[0063] (5) 7 parts of Bacillus subtilis, 7 parts of actinomycetes and 4 parts of yeast were mixed to obtain a microbial agent, 10 g of the above-mentioned mature refrigerated earthworm foregut was mixed with 2 g of the microbial agent, and then the mixture was mixed with 50 g of the above-mentioned nutrient matrix at 23°C for 1 day to obtain the soil remediation agent containing earthworms.

[0064] Example 3

[0065] (1) Collecting 1 kg of fruit and vegetable leaves and air-drying them; collecting 15 kg of livestock and poultry feces and filtering them to obtain livestock and poultry feces with a moisture content of 80-85%, and then stirring and mixing the livestock and poultry feces with fruit and vegetable leaves at a temperature of 23° C. and a humidity of 75%, and then standing for 3 days after mixing evenly to obtain a fermentation substrate;

[0066] (2) placing 100 g of Eisenia fetida in 10 kg of fermentation medium for culturing at a temperature of 15° C. and a humidity of 75% for 65 days, and obtaining mature Eisenia fetida with reproductive annuli after the culturing;

[0067] (3) dissecting the mature earthworm obtained by culture into segments to obtain the foregut of the mature earthworm, and refrigerating the foregut at 0 to 4° C. for later use;

[0068] (4) 2 kg of corn stalks were crushed, and the obtained particles were sieved to obtain corn stalk particles with a mesh size of 60 to 65, which were mixed with 50 mL of a 20% glucose solution by mass, and fermented at 35° C. for 25 days to obtain a nutrient matrix;

[0069] (5) 10 parts of Bacillus subtilis, 10 parts of actinomycetes and 3 parts of yeast were mixed to obtain a microbial agent, 20 g of the above-mentioned mature refrigerated earthworm foregut was mixed with 5 g of the microbial agent, and then the mixture was mixed with 200 g of the above-mentioned nutrient matrix at 25°C for 1 day to obtain the soil remediation agent containing earthworms.

[0070] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing a soil remediation agent containing earthworms, characterized in that: The following steps are involved: Collecting fruit and vegetable leaves and livestock and poultry excrement, and first mixing the two to obtain a fermentation substrate; placing earthworms in the fermentation substrate for culturing to obtain mature earthworms with reproductive annuli; Dissecting the mature earthworms into sections to obtain the earthworm foregut, which is then refrigerated for later use; The corn stalks are crushed and fermented to obtain a nutrient matrix; The earthworm foregut and the microbial agent are mixed for the second time, and after mixing, they are placed in the nutrient matrix for the third mixing to obtain the soil remediation agent containing earthworms.

2. The method for preparing a soil remediation agent containing earthworms according to claim 1, characterized in that: The mass ratio of fruit and vegetable leaves to livestock and poultry excrement in the first mixture is 1-5:10-15.

3. The method for preparing a soil remediation agent containing earthworms according to claim 1, characterized in that: The first mixing is performed at a temperature of 20-23° C., for 2-5 days, and at a humidity of 65-75%.

4. The method for preparing a soil remediation agent containing earthworms according to claim 1, characterized in that: The mass ratio of the earthworms to the fermentation substrate is 1-5:100; the culture conditions are as follows: the temperature is 15-20°C, the humidity is 70-80%, and the culture time is 65-75 days.

5. The method for preparing a soil remediation agent containing earthworms according to claim 1, characterized in that: The mass ratio of the earthworms to the corn stalks is 1 to 5:

20.

6. The method for preparing a soil remediation agent containing earthworms according to claim 1, characterized in that: The corn stalks have a particle size of 60-65 meshes, and the fermentation is performed by mixing the corn stalks with a glucose solution.

7. The method for preparing a soil remediation agent containing earthworms according to claim 6, characterized in that: The mass fraction of the glucose solution is 20%, and the usage ratio of the corn stalks to the glucose solution is 1-2 kg:40-50 mL.

8. The method for preparing a soil remediation agent containing earthworms according to claim 1 or claim 6, characterized in that: The fermentation conditions are as follows: temperature of 35-45° C. and fermentation time of 25-30 days.

9. The method for preparing a soil remediation agent containing earthworms according to claim 1, characterized in that: The microbial agent comprises 5-10 parts of Bacillus subtilis, 5-10 parts of actinomycetes and 3-5 parts of yeast, and the mass ratio of earthworm foregut to microbial agent in the second mixture is 10-15:2-3.

10. The method for preparing a soil remediation agent containing earthworms according to claim 1, characterized in that: The mass ratio of earthworm foregut to nutrient matrix in the third mixture is 1-3:10; the temperature of the third mixture is 20-25° C., and the time is 1-2 days.