Extraction of traditional Chinese medicine composition and application of traditional Chinese medicine composition in preparation of composition for removing cutin and improving acne

Abstract

The invention discloses extraction of a traditional Chinese medicine composition and application of the traditional Chinese medicine composition in preparation of a composition for removing cutin and improving acne, and belongs to the field of cosmetics. The preparation method comprises the following steps: firstly, extracting traditional Chinese medicines (three or more of honeysuckle, lucid ganoderma, golden cypress, centella asiatica, paris polyphylla, salvia miltiorrhiza and liquorice) to obtain a traditional Chinese medicine extract; and uniformly mixing the water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, the traditional Chinese medicine extract and water to obtain the composition for removing cutin and improving acne. Wherein the mass ratio of the water-soluble azelaic acid system to the pyridoxine or the pyridoxine derivative to the traditional Chinese medicine extract to the water is (0.5-20): (0.1-10): (0.5-10): 100. The composition prepared by the invention can improve the effects of exfoliating and improving acnes, and is exact in curative effect, safe and free of side effects.

Description

Technical Field

[0001] The invention relates to an extraction of a traditional Chinese medicine combination and application of the same in preparing a composition for exfoliating and improving acne, belonging to the field of cosmetics. Background Art

[0002] Azelaic acid is a naturally occurring straight-chain saturated dicarboxylic acid containing nine carbon atoms. Azelaic acid has good antibacterial properties and significant permeability in the skin surface. It can treat skin problems such as acne and acne. It can also balance oil secretion, stimulate hair growth, inhibit the activity of tyrosinase, reduce pigmentation, and effectively fade acne marks. Azelaic acid has local skin irritation, mainly erythema, itching, scaling and burning sensation, and its irritation mechanism is still unclear. The applicant has previously applied for a series of patents for cosmetic compositions involving azelaic acid, for example: CN117017850A relates to a cosmetic composition for external use, which comprises: azelaic acid, bisabolol, white willow bark extract, Egyptian blue water lily extract, kale leaf extract and yarrow extract; CN117530874A relates to a method for preparing a water-soluble azelaic acid system, comprising the following steps: mixing azelaic acid, salicylic acid and panthenol evenly, stirring and heating at 65-110°C for 1-8h, so that azelaic acid, salicylic acid and panthenol form a liquid organic body through intermolecular hydrogen bonds, namely, a water-soluble azelaic acid system.

[0003] Salicylic acid is a fat-soluble organic acid, mainly used to treat acne, warts and seborrheic dermatitis. Studies have shown that it is more effective than benzoyl peroxide in treating acne and blackheads; 1-4% salicylic acid removes surface keratinization, and concentrations above 5% can be used to remove deep keratinization. Salicylic acid is the mainstream of acne-removing products in the current cosmetics field, but it is difficult to dissolve in water and has strong irritation, so its application is greatly limited. Therefore, azelaic acid and salicylic acid are both raw materials with good exfoliation and acne improvement effects in this field, but their irritation and water solubility are still problems that need to be urgently solved in the current cosmetics field.

[0004] Regarding the water solubility problem, the applicant previously submitted application CN117530874A, which provides a method for preparing a water-soluble azelaic acid system, including the following steps: mixing azelaic acid, salicylic acid and panthenol evenly, stirring and heating at 65-110°C for 1-8h, so that azelaic acid, salicylic acid and panthenol form a liquid organic body through intermolecular hydrogen bonds, that is, a water-soluble azelaic acid system. Optionally, the molar ratio of azelaic acid, salicylic acid and panthenol is 1:0.3-15:1-12; the application selects salicylic acid and panthenol as compatible molecules, and mixes and heats them with azelaic acid, so that azelaic acid, salicylic acid and panthenol form a liquid organic body through intermolecular hydrogen bonds, which effectively improves the solubility of azelaic acid, and does not require a large amount of solvent, and the operation is very simple. In addition, salicylic acid as a compatible molecule can synergistically improve the exfoliating ability of azelaic acid.

[0005] Afterwards, the applicant conducted a study on the exfoliation and acne improvement efficacy of the product and found that, since the composition added azelaic acid and salicylic acid, which are components with certain irritation, although the composition also added panthenol as a deeply penetrating moisturizer and irritation improvement, the efficacy evaluation experiment found that the irritation still existed. Therefore, the technical problem to be solved by the present invention is to provide a composition for improving irritation and a preparation method thereof on the basis of the prior art, and further, the exfoliation and acne improvement effects can be improved, with definite efficacy, safety and no side effects. Summary of the invention

[0006] In order to solve the above technical problems, the applicant first tried to select and add components with oil control and soothing effects in the composition for exfoliation and acne improvement provided by application CN117530874A; the prior application CN117442509A provides a low-dose oil control and soothing composition, including azelaic acid and its derivatives, zinc salts, vitamins, wherein the vitamins can be selected as niacinamide (vitamin B3), pyridoxine (vitamin B6), etc., and the experimental group 3 of the application uses azelaic acid and pyridoxine compound to carry out relevant experimental research, and the application also mentions that vitamin B6 is one of the indispensable vitamins in human metabolism, which can inhibit sebaceous gland activity, reduce sebum secretion, participate in a variety of skin metabolism, and has multi-path help for the regulation of scalp health. Therefore, the preliminary test of the present invention first screened the relevant B vitamins, and found that the addition of pyridoxine (vitamin B6) and its derivatives to improve the effect of exfoliation and improving acne is the best, but there is still a certain irritation.

[0007] Traditional Chinese medicine is the quintessence of our country, and acne belongs to the category of lung wind acne in traditional Chinese medicine. People who are prone to acne are mostly hot constitutions and oily skin. They are prone to getting angry, and have facial redness, swelling, boils, ups and downs, and even suppuration and pain. This reflects that the damp-heat evil toxins in the body are heavier, and it is necessary to adjust the diet to clear away heat and detoxify, purge fire and eliminate acne, and clear the intestines, so as to improve the hot constitution that is prone to acne. Traditional Chinese medicine is used for exfoliation and improvement of blackheads, which often has the advantages of good safety, low irritation, and soothing. It can be seen that traditional Chinese medicine often uses clearing heat and detoxification as the main treatment principle for acne, and its selection of medicinal materials also reuses the medicinal taste of clearing heat and detoxification efficacy. In order to solve the stimulation and enhance the effect of exfoliation and improvement of acne, the applicant attempts to screen relevant components from traditional Chinese medicines for clearing heat and detoxification, and conducts evaluation of efficacy experiments and irritation experiments. Therefore, the present invention selects common single-flavor heat-clearing and detoxifying Chinese medicines in the early stage, and extracts honeysuckle, forsythia, wild chrysanthemum, phellodendron, sophora flavescens, and licorice with 80% concentration of ethanol respectively. The extracts are subjected to preliminary experiments to inhibit Propionibacterium acnes, and it is found that honeysuckle, forsythia, wild chrysanthemum, phellodendron, sophora flavescens, and licorice all have certain activity in inhibiting Propionibacterium acnes, among which the antibacterial ability of honeysuckle extract is the best and the irritation is the lowest. However, considering that honeysuckle, forsythia, and wild chrysanthemum have similar effects, and the preliminary experimental results show that honeysuckle has lower irritation than forsythia and wild chrysanthemum. Phellodendron and sophora flavescens have similar effects, and the preliminary experimental results show that phellodendron has lower irritation effect than sophora flavescens. Licorice is also often added to Chinese medicine compositions for the purpose of harmonizing the medicinal properties. Therefore, in order to simplify the composition, only honeysuckle, phellodendron and licorice with stronger activity are selected for the above six medicinal materials.

[0008] Ganoderma lucidum often has the effects of tonifying qi and improving complexion, while salvia miltiorrhiza can promote blood circulation and remove blood stasis, and is also commonly used for acne and chloasma. For pigmentation, these two herbs are often used, and Ganoderma lucidum and salvia miltiorrhiza also have a soothing effect. Acne patients often leave red acne marks after treatment. In order to alleviate the severity of acne marks, the inventor added Ganoderma lucidum and salvia miltiorrhiza, which can improve complexion and remove acne marks to a certain extent.

[0009] Centella asiatica extract can have multiple effects in cosmetics, such as antibacterial, anti-inflammatory, and promoting the synthesis of fibroblasts and collagen cells. In general, the main active ingredients of Centella asiatica extract for treating acne are asiaticoside, asiatic acid, madecassic acid and madecassin. Most of these ingredients are alcohol-soluble. For products with sensitive skin and redness, Centella asiatica extract and other ingredients, such as vitamin B5, are often used in combination to solve discomfort such as skin tingling. Paris polyphylla extract has excellent inhibitory effect on Propionibacterium acnes. When treating acne, Paris polyphylla generally has the effects of clearing away heat and detoxifying, reducing swelling and relieving pain. For the combined use of Paris polyphylla extract and Centella asiatica extract, the inventor has previously studied the products currently on the market. The Caizhiji Paris polyphylla essence under Yunnan Baiyao uses Paris polyphylla extract, Centella asiatica extract, and aloe extract for compounding to reduce skin irritation, soothe and repair, and effectively improve facial redness. Skin irritation, facial redness and other problems are also common problems after the use of products containing salicylic acid and azelaic acid. Therefore, the inventor tried to further add Centella asiatica and Paris polyphylla to honeysuckle, phellodendron amurense, liquorice, ganoderma lucidum and salvia miltiorrhiza.

[0010] Therefore, for the external Chinese herbal extracts selected by the present invention, the Chinese herbal raw materials commonly used in clinical practice to improve acne are selected, with clearing away heat and detoxifying as the main treatment principles, supplemented by medicinal materials for invigorating qi and activating blood circulation, and soothing and maintaining stability. That is, the raw materials of the composition of the Chinese herbal extracts include 3 or more of honeysuckle, ganoderma lucidum, phellodendron chinense, Centella asiatica, Paris polyphylla, Salvia miltiorrhiza and licorice.

[0011] Based on this, the present invention provides an external preparation for exfoliation and improvement of acne, which is composed of azelaic acid, salicylic acid, panthenol, pyridoxine or pyridoxine derivatives, and Chinese herbal extracts. The product of the present invention is mild and less irritating, and its effect is better than the commercially available clindamycin phosphate gel and Boleda supramolecular salicylic acid.

[0012] The first object of the present invention is to provide a method for preparing a Chinese medicine extract, comprising the following steps:

[0013] The Chinese medicine components are mixed, crushed, and sieved to obtain a Chinese medicine mixed powder; then the Chinese medicine mixed powder is added to an ethanol aqueous solution with a volume fraction of 75-85% for extraction and filtering; then the drug residues are repeatedly extracted, the extracts are combined, concentrated, and dried to obtain a Chinese medicine extract;

[0014] Among them, the Chinese medicinal components include 3 or more of honeysuckle, Ganoderma lucidum, Phellodendron chinense, Centella asiatica, Paris polyphylla, Salvia miltiorrhiza and licorice.

[0015] In one embodiment of the present invention, the dosage ratio of the mixed Chinese medicine powder to the ethanol aqueous solution with a volume fraction of 75-85% is 1 g: 3-10 mL.

[0016] In one embodiment of the present invention, when the Chinese medicinal components are honeysuckle, ganoderma lucidum and Centella asiatica, the mass ratio of the three is 20-30:10-20:5-10.

[0017] In one embodiment of the present invention, when the Chinese medicinal components are honeysuckle, ganoderma lucidum, Centella asiatica and phellodendron chinense, the mass ratio of the four is 20-30:10-15:5-10:10-15.

[0018] In one embodiment of the present invention, when the Chinese medicinal components are honeysuckle, ganoderma lucidum, Centella asiatica, phellodendron amurense and salvia miltiorrhiza, the mass ratio of the five is 20-30:10-15:5-10:10-15:5-10.

[0019] In one embodiment of the present invention, when the Chinese medicinal components are honeysuckle, ganoderma lucidum, Centella asiatica, phellodendron chinense, salvia miltiorrhiza and paridis yunnanensis, the mass ratio of the six is ​​20-30:10-15:5-10:10-15:5-10:5-10.

[0020] In one embodiment of the present invention, when the Chinese medicinal components are honeysuckle, ganoderma lucidum, Centella asiatica, phellodendron, salvia miltiorrhiza, paridis yunnanensis and liquorice, the mass ratio of the seven is 20-30:10-15:5-10:10-15:5-10:5-10:3-5.

[0021] In one embodiment of the present invention, the extraction is performed at 20-30° C. (normal temperature) for 30-120 min.

[0022] In one embodiment of the present invention, the extraction can be carried out by ultrasonic extraction, reflux extraction, cold soaking extraction, etc.

[0023] In one embodiment of the present invention, the extraction is repeated 1-4 times.

[0024] The second object of the present invention is the Chinese herbal medicine extract prepared by the method of the present invention.

[0025] The third object of the present invention is to provide a composition for exfoliating and improving acne, the components of which include a water-soluble azelaic acid system, pyridoxine or a pyridoxine derivative, a Chinese medicine extract, and water; wherein the mass ratio of the water-soluble azelaic acid system, pyridoxine or a pyridoxine derivative, the Chinese medicine extract, and water is 0.5-20:0.1-10:0.5-10:100.

[0026] In one embodiment of the present invention, the mass ratio of the water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, traditional Chinese medicine extracts, and water is 0.5-10:0.1-7:0.5-7:100; preferably, the mass ratio of the water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, traditional Chinese medicine extracts, and water is 1-7:0.1-4:0.5-5:100; further preferably, the mass ratio of the water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, traditional Chinese medicine extracts, and water is 2-5:0.2-2:1-3:100; most preferably, the mass ratio of the water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, traditional Chinese medicine extracts, and water is 3:0.5:2:100 or 2:0.2:1:100.

[0027] In one embodiment of the present invention, the pyridoxine derivative is pyridoxine tri-hexyldecanoate.

[0028] In one embodiment of the present invention, the water-soluble azelaic acid system is prepared using azelaic acid, salicylic acid and panthenol, wherein the molar ratio of azelaic acid, salicylic acid and panthenol is 1:0.3-15:1-12; preferably, the molar ratio of azelaic acid, salicylic acid and panthenol is 1:1.5:2 or 1:1:4; panthenol includes but is not limited to DL-panthenol.

[0029] In one embodiment of the present invention, the preparation method of the water-soluble azelaic acid system is as follows:

[0030] Mix azelaic acid, salicylic acid and panthenol evenly, and heat with stirring at 65-110° C. for 1-8 hours, so that azelaic acid, salicylic acid and panthenol form a liquid organic body through intermolecular hydrogen bonds, i.e., a water-soluble azelaic acid system;

[0031] The rotation speed of the stirring and heating is 300-1000 rpm; the atmosphere of the stirring and heating is air, argon or carbon dioxide.

[0032] In one embodiment of the present invention, auxiliary materials may be added to the composition for exfoliation and improvement of acne, and the dressing includes but is not limited to one or more of preservatives, pH regulators, and flavors.

[0033] In one embodiment of the present invention, the composition for exfoliating and improving acne is a liquid preparation.

[0034] The fourth object of the present invention is to provide a method for preparing a composition for exfoliation and improving acne, comprising the following steps:

[0035] The water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, Chinese herbal medicine extracts and water are uniformly mixed to obtain a composition for exfoliating and improving acne.

[0036] A fifth object of the present invention is to provide a cosmetic, which uses the composition for exfoliating and improving acne of the present invention.

[0037] The sixth object of the present invention is to use the composition for exfoliation and improving acne in daily chemical products.

[0038] The seventh object of the present invention is to provide a method for improving the exfoliation and acne improvement performance of cosmetics, wherein the method uses the composition for exfoliation and acne improvement of the present invention.

[0039] [Beneficial Effects]

[0040] (1) Azelaic acid, salicylic acid, and panthenol in the water-soluble azelaic acid system of the present invention interact with each other through supramolecular hydrogen bonds to form an organic whole, thereby effectively improving the water solubility of azelaic acid and enhancing the exfoliating and acne-improving effects of azelaic acid.

[0041] (2) The present invention contains raw materials such as salicylic acid and azelaic acid. After adding pyridoxine or pyridoxine derivatives and Chinese herbal extracts, the efficacy of exfoliation and improvement of acne is enhanced, and problems such as skin irritation and facial redness are also alleviated to a certain extent.

[0042] (3) The process flow of the composition for exfoliation and acne improvement of the present invention is simple, safe, and easy to operate, and other added raw materials are also commonly used raw materials in the field, with reasonable prices, can be directly applied to cosmetics, and are easy to promote.

[0043] (4) The composition for exfoliation and improvement of acne prepared by the present invention is mild and less irritating, and its effect is better than the commercially available clindamycin phosphate gel and Boleda supramolecular salicylic acid. DETAILED DESCRIPTION

[0044] The preferred embodiments of the present invention are described below. It should be understood that the embodiments are for better explaining the present invention and are not used to limit the present invention.

[0045] The raw materials used in the examples are:

[0046] Honeysuckle, Ganoderma lucidum, Phellodendron chinense, Centella asiatica, Paris polyphylla, Salvia miltiorrhiza, Licorice: commercially available;

[0047] Pyridoxine; ≥98%, commercially available;

[0048] Pyridoxine tri-hexyldecanoate: 98%, commercially available;

[0049] Azelaic acid: particle size 500nm-100μm, particle size has no effect, the particle size selected in the embodiment and comparative example is 30μm, commercially available;

[0050] Panthenol: D-panthenol or DL-panthenol, both of which can be used in the examples and comparative examples, are commercially available.

[0051] Example 1

[0052] A method for preparing a water-soluble azelaic acid system comprises the following steps:

[0053] Weigh 0.01 mol of azelaic acid, 0.015 mol of salicylic acid and 0.02 mol of DL-panthenol, stir evenly and place in a sealed reaction container. Place the reaction container in an argon protective atmosphere, keep the reaction container stirring and heating at 600 rpm and 95°C for 3 hours, then cool the reaction container to room temperature, so that azelaic acid, salicylic acid and panthenol form a liquid organic body through intermolecular hydrogen bonds, i.e., a water-soluble azelaic acid system.

[0054] The water-soluble azelaic acid system is uniform and transparent;

[0055] The azelaic acid content in the water-soluble azelaic acid system is 23 wt %, and the pH value measured by a pH meter is 4.37;

[0056] The water-soluble azelaic acid system was diluted with water until the azelaic acid accounted for 3% of the total mass fraction, and the system was still homogeneous and transparent;

[0057] The water-soluble azelaic acid system and the diluted system were subjected to high and low temperature cycle tests (cycled and stored at -5°C and 40°C for 15 times), and finally both systems were able to maintain a stable and uniform liquid state without phase separation or solid precipitation.

[0058] Example 2

[0059] A method for preparing a water-soluble azelaic acid system comprises the following steps:

[0060] Weigh 0.01 mol of azelaic acid, 0.01 mol of salicylic acid and 0.04 mol of D-panthenol, stir evenly and place in a sealed reaction container. Place the reaction container in a natural air atmosphere, keep the reaction container stirring and heating at 500 rpm and 85°C for 2 hours, then cool the reaction container to room temperature, so that azelaic acid, salicylic acid and panthenol form a liquid organic body through intermolecular hydrogen bonds, i.e., a water-soluble azelaic acid system.

[0061] The water-soluble azelaic acid system is uniform and transparent;

[0062] The azelaic acid content in the water-soluble azelaic acid system is 16 wt %, and the pH value measured by a pH meter is 5.03;

[0063] The water-soluble azelaic acid system was diluted with water until the azelaic acid accounted for 2% of the total mass fraction, and the system was still homogeneous and transparent;

[0064] The water-soluble azelaic acid system and the diluted system were subjected to high and low temperature cycle tests (cycled and stored at -5°C and 40°C for 15 times), and finally both systems were able to maintain a stable and uniform liquid state without phase separation or solid precipitation.

[0065] Example 3

[0066] A method for preparing a Chinese medicine extract comprises the following steps:

[0067] Mix 30g of honeysuckle, 20g of ganoderma lucidum, and 8g of Centella asiatica, crush, and sieve to obtain a Chinese medicine mixed powder;

[0068] 58 g of the mixed Chinese medicine powder was added to 600 mL of 80% ethanol aqueous solution, and ultrasonically extracted for 60 min at room temperature, and filtered;

[0069] The residue was extracted twice, a total of three times, and the extracts were combined, concentrated under reduced pressure, and dried to obtain the Chinese medicine extract.

[0070] Example 4

[0071] A method for preparing a Chinese medicine extract comprises the following steps:

[0072] Mix 30g of honeysuckle, 10g of ganoderma lucidum, 8g of Centella asiatica and 10g of Phellodendron chinense, grind and sieve to obtain a Chinese medicine mixed powder;

[0073] 58 g of the mixed Chinese medicine powder was added to 600 mL of 80% ethanol aqueous solution, and ultrasonically extracted for 60 min at room temperature, and filtered;

[0074] The residue was extracted twice, a total of three times, and the extracts were combined, concentrated under reduced pressure, and dried to obtain the Chinese medicine extract.

[0075] Example 5

[0076] A method for preparing a Chinese medicine extract comprises the following steps:

[0077] Mix 28g of honeysuckle, 10g of ganoderma lucidum, 5g of Centella asiatica, 10g of Phellodendron chinense, and 5g of Salvia miltiorrhiza, grind, and sieve to obtain a Chinese medicine mixed powder;

[0078] 58 g of the mixed Chinese medicine powder was added to 600 mL of 80% ethanol aqueous solution, and ultrasonically extracted for 60 min at room temperature, and filtered;

[0079] The residue was extracted twice, a total of three times, and the extracts were combined, concentrated under reduced pressure, and dried to obtain the Chinese medicine extract.

[0080] Example 6

[0081] A method for preparing a Chinese medicine extract comprises the following steps:

[0082] Mix 23g of honeysuckle, 10g of ganoderma lucidum, 5g of Centella asiatica, 10g of Phellodendron chinense, 5g of Salvia miltiorrhiza, and 5g of Paris polyphylla, grind, and sieve to obtain a Chinese medicine mixed powder;

[0083] 58 g of the mixed Chinese medicine powder was added to 600 mL of 80% ethanol aqueous solution, and ultrasonically extracted for 60 min at room temperature, and filtered;

[0084] The residue was extracted twice, a total of three times, and the extracts were combined, concentrated under reduced pressure, and dried to obtain the Chinese medicine extract.

[0085] Example 7

[0086] A method for preparing a Chinese medicine extract comprises the following steps:

[0087] Mix 20g of honeysuckle, 10g of ganoderma lucidum, 5g of centella asiatica, 10g of phellodendron, 5g of salvia miltiorrhiza, 5g of parsley, and 3g of liquorice, grind, and sieve to obtain a Chinese medicine mixed powder;

[0088] 58 g of the mixed Chinese medicine powder was added to 600 mL of 80% ethanol aqueous solution, and ultrasonically extracted for 60 min at room temperature, and filtered;

[0089] The residue was extracted twice, a total of three times, and the extracts were combined, concentrated under reduced pressure, and dried to obtain the Chinese medicine extract.

[0090] Example 8

[0091] A method for preparing a Chinese medicine extract comprises the following steps:

[0092] Mix 30g of honeysuckle, 15g of ganoderma lucidum, 15g of phellodendron, 10g of centella asiatica, 10g of parsley, 10g of salvia miltiorrhiza, and 5g of liquorice, grind, and sieve to obtain a Chinese medicine mixed powder;

[0093] 95 g of the mixed powder of traditional Chinese medicine was added into 600 mL of 80% ethanol aqueous solution, and ultrasonically extracted for 60 min at room temperature, and filtered;

[0094] The residue was extracted twice, a total of three times, and the extracts were combined, concentrated under reduced pressure, and dried to obtain the Chinese medicine extract.

[0095] Example 9

[0096] A method for preparing a Chinese medicine extract comprises the following steps:

[0097] Mix 25g of honeysuckle, 12g of ganoderma lucidum, 12g of phellodendron, 8g of centella asiatica, 8g of parsnip, 8g of salvia miltiorrhiza, and 4g of liquorice, grind, and sieve to obtain a Chinese medicine mixed powder;

[0098] 77 g of the mixed Chinese medicine powder was added to 500 mL of 80% ethanol aqueous solution, and ultrasonically extracted for 60 min at room temperature, and filtered;

[0099] The residue was extracted twice, a total of three times, and the extracts were combined, concentrated under reduced pressure, and dried to obtain the Chinese medicine extract.

[0100] Example 10

[0101] A method for preparing a composition for exfoliation and improving acne, comprising the following steps:

[0102] The water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, Chinese herbal medicine extracts and water are uniformly mixed to obtain a composition for exfoliation and improving acne;

[0103] The specific components and proportions are shown in Table 1:

[0104] Table 1

[0105]

[0106] Example 11 Antibacterial effect

[0107] Evaluation No. 1-15 for the antibacterial effect on Propionibacterium acnes.

[0108] (1) Experimental strains:

[0109] Propionibacterium acnes ATCC6919: purchased from Shanghai Huzheng Biotechnology Co., Ltd.

[0110] (2) Experimental methods:

[0111] a. Preparation of Propionibacterium acnes bacteria:

[0112] The Propionibacterium acnes stored on the slant was transferred to liquid anaerobic culture medium and cultured at 37°C for 36 hours under anaerobic conditions. The bacterial suspension was diluted with sterile saline to a concentration of 1×10 7 CFU / mL to obtain bacterial suspension.

[0113] b. Antibacterial test:

[0114] The agar diffusion method was used for determination. A 12-cm-diameter plate was taken, 10 mL of culture medium was added, and 100 μL of the above bacterial suspension was aspirated under aseptic operation conditions and evenly spread on the cooled and solidified culture medium.

[0115] c. Place a sterile Oxford cup with a diameter of 6 mm on the agar plate with sterile tweezers and press lightly to ensure good contact. Take 200 μL of the composition and add it to the Oxford cup respectively. The blank control group is water. Incubate at 37°C for 72 hours under anaerobic conditions, measure the diameter of the effective inhibition zone of the test solution in each culture dish, and calculate the average value after repeating the measurement 4 times for each concentration.

[0116] Antibacterial effect judgment criteria:

[0117] If the diameter of the inhibition zone is >20mm, it is considered highly sensitive; if it is 10mm-20mm, it is considered moderately sensitive; if it is <10mm, it is considered lowly sensitive; and if no inhibition zone is produced, it is considered resistant.

[0118] The antibacterial results are shown in Table 2 below (unit: mm):

[0119] Table 2

[0120] Serial number Average value of 4 times (mm) Blank control group 6(cup diameter) 1 22.5 2 24.6 3 25.8 4 26.7 5 29.4 6 26.4 7 27.5 8 26.7 9 29.2 10 28.5 11 28.2 12 29.6 13 14.6 14 16.4 15 19.3

[0121] It can be seen from Table 2 that No. 1-12 all have an inhibitory effect on Propionibacterium acnes, and the antibacterial effect will decrease if the component is missing;

[0122] The average diameters of the inhibition zones of No. 1-12 to Propionibacterium acnes were all greater than 20 mm, indicating that they were highly sensitive, and No. 13-15 were moderately sensitive to Propionibacterium acnes.

[0123] According to statistical analysis, No. 1-12 has a significant improvement effect compared to No. 13-15, and the P value is less than 0.01.

[0124] Through the above-mentioned antibacterial effect test on Propionibacterium acnes, the inventor unexpectedly found that on the basis of the water-soluble azelaic acid system, after compounding with the extract and pyridoxine, it has a synergistic effect on inhibiting Propionibacterium acnes.

[0125] Example 12 Irritation, effectiveness and functional effects

[0126] The irritation, effectiveness and functional effect evaluation tests were conducted on No. 5-7.

[0127] The test method is as follows: 60 acne patients aged 20-45 years old were selected, half of them were male and half were female, among whom the patients often suffered from acne and the acne area was large.

[0128] The preparations obtained by No. 5-7 were used, and the positive drugs clindamycin phosphate gel and Boleda supramolecular salicylic acid were used as comparisons;

[0129] 40 people were selected, 10 people were selected for clindamycin phosphate gel, and 10 people were also selected for Boleda supramolecular salicylic acid;

[0130] Apply once every morning and evening after cleansing for 10 consecutive days;

[0131] On the 5th and 10th days, patients were surveyed on the effectiveness and irritation of the product through questionnaires.

[0132] The specific standards are as follows:

[0133] Table 3 Validity criteria

[0134]

[0135] Table 4 Irritation Standards

[0136] Irritant standard Obvious stimulation Stinging, noticeable redness, peeling, dryness More exciting There is a stinging sensation, obvious redness, a little peeling, and dryness Milder Slight tingling sensation, no peeling, no redness, and no obvious dryness mild The product is comfortable, non-irritating, non-reddening, and non-drying

[0137] The results are shown in Table 5:

[0138] It can be seen from Table 5 that the products prepared with No. 5, 6, and 7 have good effects, and their irritation is significantly lower than that of clindamycin phosphate gel and Boleda supramolecular salicylic acid. They are mild and effective, and their effects are better than those of clindamycin phosphate gel and Boleda supramolecular salicylic acid commonly used in clinical practice.

[0139] Table 5 Comparison of patients' use

[0140]

[0141] Example 13 Exfoliation Effect

[0142] The exfoliating effect of No. 5, 6, 13, and 14 was tested.

[0143] The specific test method is similar to the applicant's prior patent CN117530874A as a whole, and is as follows:

[0144] The German CK skin texture scanner Visioscan (VC20) was used to test the stratum corneum of human skin, including initial value testing, product use, efficacy testing, and statistical analysis. The samples were tested in accordance with the physical and chemical testing methods in Chapter 4 of the "Technical Specifications for Safety of Cosmetics".

[0145] (1) Test subjects and test conditions:

[0146] In a constant temperature test environment with a temperature of (21±1)℃ and a humidity of (40-60)%, 30 volunteers with more stratum corneum at the elbows were selected;

[0147] Inclusion criteria:

[0148] ① Aged 20-45, all indoor workers (except pregnant or breastfeeding women);

[0149] ② Those without severe systemic diseases, immunodeficiency or autoimmune diseases;

[0150] ③ Those without active allergic diseases;

[0151] ④ Those who are not highly sensitive;

[0152] ⑤ Those who have not used hormone drugs or immunosuppressants in the past month;

[0153] ⑥ Those who have not participated in other clinical trials at the current or recent three months;

[0154] Termination and elimination criteria:

[0155] ① The volunteer requests to terminate the trial for personal reasons;

[0156] ② The volunteer has an adverse reaction and cannot continue the trial;

[0157] ③ The volunteers have poor compliance, for example, they use other skin care products that may affect the test.

[0158] (2) Data testing:

[0159] 30 volunteers were selected for the test according to the random double-blind principle. The skin with more stratum corneum around the left or right elbow was randomly selected as the test area. The test area was positioned and the positioning mark was kept on the test area at all times. During the test, no other shower gel or cosmetics or other products that may affect the test results were used on the test area. Volunteers No. 5, 6, 7, 13, and 14 applied the product to the same position for 14 consecutive days, once in the morning and once in the evening every day. During the test, no other cosmetics or toiletries with exfoliating effect were used.

[0160] The stratum corneum exfoliation number (SEsc) of volunteers No. 5, 6, 7, 13, and 14 on the 0th, 7th, and 14th days were tested respectively. The test area was kept dry one hour before each test. During the test, the subjects were required to sit quietly for at least 20 minutes in a constant temperature and humidity environment (20-22°C, relative humidity 40%-60%). The measurement personnel measured the stratum corneum exfoliation number (SEsc) value of the subjects' fixed area, and each measurement area was measured 3 times to take the average value. The smaller the stratum corneum exfoliation number (SEsc) value, the less stratum corneum there is in the test area, and the better the exfoliation effect.

[0161] (3) Test results:

[0162] Table 6 Cuticle shedding effect

[0163]

[0164] From Table 6, it can be seen that the products prepared with No. 5, 6, and 7 reduced the number of stratum corneum exfoliation (SEsc) after being used on volunteers, and the number of stratum corneum exfoliation (SEsc) was reduced by more than 24% after 14 days, indicating that the products prepared with No. 5, 6, and 7 have a good effect of reducing stratum corneum exfoliation.

[0165] Although the present invention has been disclosed as above in the form of a preferred embodiment, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the claims.

Claims

1. A method for preparing a Chinese medicine extract, characterized in that: The steps include: The Chinese medicine components are mixed, crushed, and sieved to obtain a Chinese medicine mixed powder; then the Chinese medicine mixed powder is added to an ethanol aqueous solution with a volume fraction of 75-85% for extraction and filtering; then the drug residues are repeatedly extracted, the extracts are combined, concentrated, and dried to obtain a Chinese medicine extract; Among them, the Chinese medicinal components include 3 or more of honeysuckle, Ganoderma lucidum, Phellodendron chinense, Centella asiatica, Paris polyphylla, Salvia miltiorrhiza and licorice.

2. The method according to claim 1, characterized in that The dosage ratio of the Chinese medicine mixed powder to the ethanol aqueous solution with a volume fraction of 75-85% is 1g:3-10mL.

3. The Chinese medicine extract prepared by the method according to claim 1 or 2.

4. A composition for exfoliating and improving acne, characterized in that: Its components include water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, the Chinese herbal extract according to claim 3, and water; wherein the mass ratio of the water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, the Chinese herbal extract, and water is 0.5-20:0.1-10:0.5-10:

100.

5. The composition for exfoliating and improving acne according to claim 4, characterized in that: When the traditional Chinese medicine components include honeysuckle, ganoderma lucidum, Centella asiatica, phellodendron chinense, salvia miltiorrhiza, paridis rhizome and liquorice, the mass ratio of the seven is 20-30:10-15:5-10:10-15:5-10:5-10:3-5.

6. The composition for exfoliating and improving acne according to claim 4, characterized in that: The preparation method of the water-soluble azelaic acid system is as follows: Mix azelaic acid, salicylic acid and panthenol evenly, and heat with stirring at 65-110° C. for 1-8 hours, so that azelaic acid, salicylic acid and panthenol form a liquid organic body through intermolecular hydrogen bonds, namely, a water-soluble azelaic acid system.

7. A method for preparing the composition for exfoliation and improving acne according to claims 4-6, characterized in that: The steps include: The water-soluble azelaic acid system, pyridoxine or pyridoxine derivatives, Chinese herbal medicine extracts and water are uniformly mixed to obtain a composition for exfoliating and improving acne.

8. A cosmetic, characterized in that: The composition for exfoliating and improving acne as described in claims 4 to 6 is used.

9. Use of the composition for exfoliation and improving acne according to claims 4-6 in daily chemical products.

10. A method for improving the exfoliation and acne-improving performance of cosmetics, characterized in that: The method adopts the composition for exfoliation and improving acne as described in claims 4-6.

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