Organ-like culture device and method

Abstract

The invention relates to the field of medical equipment, in particular to an organ-like culture device and method.The base is provided with a rotating disc driven by a power device to rotate, the top of the rotating disc is provided with multiple culture mechanisms, each culture mechanism comprises a culture groove, the top of the rotating disc is provided with a liquid inlet hole and a liquid outlet hole, and the rotating disc is internally provided with a liquid inlet channel communicating with the liquid inlet hole and the culture groove; a liquid outlet channel communicated with the liquid outlet holes and the culture grooves is also arranged in the rotating disc; a liquid changing mechanism is arranged on the base located on the side of the rotary disc and comprises a vertical frame, a mounting seat driven by a first telescopic component to ascend and descend is arranged on the vertical frame, a liquid injection pipe is fixedly mounted on the mounting seat, and a liquid pumping pipe is further fixedly mounted on the mounting seat; when the cell culture fluid in the culture tank needs to be replaced, a new cell culture fluid is injected into the culture tank through the fluid injection pipe, and an old cell culture fluid in the culture tank is pumped out through the fluid pumping pipe, so that the dynamic replacement of the cell culture fluid in the culture tank is realized.

Description

Technical Field

[0001] The present invention relates to the field of medical equipment, and in particular to an organoid culture device and method. Background Art

[0002] Organoids are three-dimensional (3D) cell cultures that contain some of the key features of the organs they represent. These in vitro culture systems include a self-renewing stem cell population that can differentiate into multiple organ-specific cell types, have similar spatial organization to the corresponding organ, and can recapitulate some of the functions of the corresponding organ, thus providing a highly physiologically relevant system.

[0003] Existing organoid culture devices, such as the invention patent with application number CN202110960051.9, discloses an organoid culture chip and culture method, wherein the organoid culture chip comprises: a cell culture plate; an organoid culture device, disposed in the cell culture plate; a culture medium reservoir is formed between the organoid culture device and the cell culture plate; the organoid culture device comprises: an organoid culture device body, an organoid culture chamber disposed in the organoid culture device body, and side holes disposed on the side walls of the organoid culture device body. The organoid culture chamber is connected to the side holes on the side walls to form a perfusion channel, which can realize high-throughput, one-step dynamic perfusion culture of organoids with uniform morphology, and can dynamically observe the entire process of organoid growth and development in situ.

[0004] However, the above-mentioned organoid culture apparatus does not have a structure that facilitates the replacement or irrigation of multiple culture medium reservoirs, which is inconvenient to use and needs to be improved. Summary of the invention

[0005] The object of the present invention is to provide an organoid culture device and method, which can automatically replace the liquid in the culture tank to solve the defects mentioned in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] An organoid culture device comprises a base, wherein a turntable driven by a power device and rotating around its own axis is provided on the base, a plurality of groups of culture mechanisms evenly spaced around the axis are provided on the top of the turntable, the culture mechanisms comprise a culture tank located on the top of the turntable, an organoid carrying device is provided in the culture tank, a liquid inlet hole and a liquid outlet hole are also provided on the top of the turntable, a liquid inlet channel connecting the liquid inlet hole and the culture tank is provided in the turntable, and a liquid outlet channel connecting the liquid outlet hole and the culture tank is also provided in the turntable; a liquid exchange mechanism is provided on the base located on the side of the turntable, the liquid exchange mechanism comprises a stand fixedly mounted on the base, a mounting seat driven to rise and fall by a first telescopic component is provided on the stand, a liquid injection tube matching the liquid inlet hole is fixedly mounted on the mounting seat, and a liquid extraction tube matching the liquid outlet hole is also fixedly mounted on the mounting seat; when the liquid inlet hole of one group of the culture mechanisms rotates to directly below the liquid injection tube, the corresponding liquid outlet hole rotates to directly below the liquid extraction tube.

[0008] As a further improvement, the top of the turntable is provided with a slide groove extending radially thereof, the slide groove corresponding to the culture mechanism one by one, a baffle is slidably installed in the slide groove for closing the corresponding liquid inlet and liquid outlet of the culture mechanism, and an annular plate is fixedly installed on the outer side of the turntable for preventing the baffle from detaching from the corresponding slide groove; when the baffle is in contact with the inner wall of the annular plate, the baffle closes the corresponding liquid inlet and liquid outlet, and the turntable is provided with a spring for pushing the baffle to fit onto the annular plate.

[0009] As a further improvement, the baffle is provided with a protruding plate extending in a direction away from the axis of the turntable, and the annular plate is provided with a notch for the protruding plate to pass through; the vertical frame is also provided with a push plate driven by a second telescopic component and moving radially along the turntable, and the protruding plate and the push plate are located at the same height.

[0010] As a further improvement, an infrared transmitter is fixedly mounted on the stand, an infrared receiver matching the infrared transmitter is fixedly mounted on the annular plate, the infrared receiver corresponds one to one with the culture mechanism, and when the infrared receiver is aligned with the infrared transmitter, the corresponding liquid inlet of the culture mechanism rotates to be directly below the injection tube, the corresponding liquid outlet rotates to be directly below the liquid extraction tube, and the corresponding convex plate is aligned with the push plate.

[0011] As a further improvement, a sliding rod parallel to the sliding groove is fixedly mounted on the baffle, and a positioning hole for inserting the sliding rod is provided at one end of the sliding groove close to the center of the turntable, and one end of the spring abuts against the inner wall of the positioning hole, and the other end abuts against the end of the sliding rod away from the baffle.

[0012] As a further improvement, a vertically extending slide rail is fixedly mounted on the stand, and a sliding block matching the slide rail is fixedly mounted on the mounting seat.

[0013] As a further improvement, a cover plate is provided on the top of the turntable to cover the multiple culture tanks.

[0014] As a further improvement, the liquid inlet channel and the liquid outlet channel are respectively connected to opposite sides of the culture tank.

[0015] An organoid culture method using the organoid culture device, the method comprising the following steps:

[0016] S1, sterilizing and disinfecting the organoid culture device;

[0017] S2, placing the organoid carrier device inoculated with the organoid in the culture tank;

[0018] S3, adding cell culture fluid into the culture tank;

[0019] S4, when the cell culture fluid in the culture tank needs to be replaced, the culture mechanism is driven by the turntable to rotate to the fluid replacement mechanism, and the injection tube and the extraction tube are driven by the mounting seat to be inserted into the liquid inlet and the liquid outlet respectively, and new cell culture fluid is injected into the culture tank through the injection tube, and the old cell culture fluid in the culture tank is extracted through the extraction tube.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. When the cell culture fluid in the culture tank needs to be replaced, the motor drives the turntable to rotate, so that a group of culture mechanisms rotate to the liquid replacement mechanism, and then the mounting seat moves downward to drive the lower end of the injection tube to be inserted into the liquid inlet hole, and the lower end of the extraction tube to be inserted into the liquid outlet hole, and new cell culture fluid is injected into the culture tank through the injection tube, and the old cell culture fluid in the culture tank is extracted through the extraction tube, thereby realizing the dynamic replacement of the cell culture fluid in the culture tank, and making the replacement of the cell culture fluid more convenient;

[0022] 2. When replacing the cell culture fluid in the culture tank, the push plate pushes the baffle plate toward the center of the turntable to open the liquid inlet and outlet holes. Or after the cell culture fluid is replaced, the push plate returns to its original position, and the spring pushes the baffle plate to re-close the liquid inlet and outlet holes, which can reduce the cell culture fluid in the liquid inlet or outlet holes from being contaminated by the outside world. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0024] Figure 1 is a schematic structural diagram of an embodiment of the present invention;

[0025] Figure 2 is an exploded schematic diagram of a base according to an embodiment of the present invention;

[0026] Figure 3 is a schematic structural diagram of a turntable according to an embodiment of the present invention;

[0027] Figure 4 is a schematic diagram of the structure inside the turntable of an embodiment of the present invention;

[0028] Figure 5 is an exploded schematic diagram of a turntable according to an embodiment of the present invention;

[0029] Figure 6 is a schematic structural diagram of a liquid replacement mechanism according to an embodiment of the present invention;

[0030] Figure 7 Flow chart of the organoid culture method according to an embodiment of the present invention.

[0031] In the figure: 1-base; 2-turntable; 3-motor; 4-culture mechanism; 5-culture tank; 6-organoid carrying device; 7-culture chamber; 8-liquid hole; 9-liquid inlet hole; 10-liquid outlet hole; 11-liquid inlet channel; 12-liquid outlet channel; 13-liquid exchange mechanism; 14-stand; 15-annular plate; 16-mounting seat; 17-slide rail; 18-sliding block; 19-first electric push rod; 20-fixed seat; 21-liquid injection tube; 22-liquid extraction tube; 23-slide groove; 24-baffle; 25-spring; 26-slide rod; 27-positioning hole; 28-convex plate; 29-notch; 30-push plate; 31-guide rod; 32-second electric push rod; 33-infrared transmitter; 34-infrared receiver; 35-cover plate. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] like Figures 1 to 7 As shown, an organoid culture device includes a base 1, on which is provided a turntable 2 driven by a power device and rotating around its own axis. A motor 3 is installed in the base 1, and the rotating shaft of the motor 3 extends upward to the top of the base 1. The turntable 2 is coaxially fixedly mounted on the rotating shaft of the motor 3.

[0034] like Figure 3 and Figure 4 As shown, a plurality of culture mechanisms 4 are arranged at even intervals around the axis at the top of the turntable 2, and the culture mechanisms 4 include a culture tank 5 located at the top of the turntable 2, and an organoid carrier 6 is arranged in the culture tank 5. The culture tank 5 is circular, and the organoid carrier 6 is in the shape of a long strip extending along the diameter of the culture tank 5. The organoid carrier 6 is clamped in the culture tank 5, and a plurality of culture chambers 7 are arranged on the top of the organoid carrier 6 along its length direction. The organoid carrier 6 is also provided with a liquid through hole 8 which transversely penetrates the culture chamber 7, and the liquid through hole 8 connects the corresponding culture chamber 7 and the culture tank 5 located on the opposite sides of the organoid carrier 6, so that the cell culture fluid can flow between the culture tank 5 and the culture chamber 7. A liquid inlet 9 and a liquid outlet 10 are also provided on the top of the turntable 2. The liquid inlet 9 and the liquid outlet 10 are both located on the side of the culture tank 5 away from the center of the turntable 2. A liquid inlet channel 11 is provided in the turntable 2, connecting the bottom of the liquid inlet 9 and one side of the bottom of the culture tank 5. A liquid outlet channel 12 is also provided in the turntable 2, connecting the bottom of the liquid outlet 10 and one side of the bottom of the culture tank 5. In order to prevent the cell culture fluid from forming a short flow in the culture tank 5, the liquid inlet channel 11 and the liquid outlet channel 12 are respectively connected to the opposite sides of the culture tank 5. Specifically, the liquid inlet channel 11 and the liquid outlet channel 12 are respectively connected to the culture tank 5 on the opposite sides of the organoid carrier 6, thereby preventing the cell culture fluid injected through the liquid inlet channel 11 from being immediately discharged from the liquid outlet channel 12.

[0035] like Figure 1 and Figure 6 As shown, a liquid changing mechanism 13 is provided on the base 1 located at the rear side of the turntable 2. The liquid changing mechanism 13 includes a stand 14 fixedly installed on the base 1 by bolts, and a mounting seat 16 driven to rise and fall by a first telescopic component is provided on the stand 14. Specifically, two slide rails 17 arranged in parallel on the left and right are fixedly installed on the front side of the stand 14 by bolts, and the slide rails 17 extend vertically. A slider 18 matching the slide rails 17 is fixedly installed on the rear side of the mounting seat 16 by bolts, thereby improving the sliding stability of the mounting seat 16. The first telescopic component is a first electric push rod 19, and a fixing seat 20 is fixedly installed on the top of the front side of the stand 14 by bolts. The main body of the first electric push rod 19 is fixedly installed on the fixing seat 20 by bolts. The push rod of the first electric push rod 19 passes downward through the fixing seat 20 and is fixedly connected to the mounting seat 16 by bolts, so that the mounting seat 16 is driven to slide up and down along the slide rails 17 by the telescopic movement of the first electric push rod 19.

[0036] The lower end of the mounting seat 16 is provided with a horizontal portion extending forward, on which a liquid injection tube 21 matching the liquid inlet hole 9 is fixedly mounted, and a liquid extraction tube 22 matching the liquid outlet hole 10 is also fixedly mounted, the liquid injection tube 21 and the liquid extraction tube 22 are arranged side by side, the liquid injection tube 21 and the liquid extraction tube 22 are both extended vertically, the upper end of the liquid injection tube 21 is connected to the liquid injection pipeline, and the upper end of the liquid extraction tube 22 is connected to the liquid extraction pipeline, and the liquid injection pipeline and the liquid extraction pipeline are respectively provided with peristaltic pumps. When the liquid inlet hole 9 of one group of the culture mechanisms 4 rotates to the right below the liquid injection tube 21, the corresponding liquid outlet hole 10 rotates to the right below the liquid extraction tube 22, so that when the mounting seat 16 moves downward, the lower end of the liquid injection tube 21 can be driven to be inserted into the liquid inlet hole 9 and the lower end of the liquid extraction tube 22 can be driven to be inserted into the liquid outlet hole 10 at the same time.

[0037] like Figure 5 As shown, a slide groove 23 extending along its radial direction is provided on the top of the turntable 2, and the slide groove 23 corresponds to the culture mechanism 4 one by one, and the liquid inlet hole 9 and the liquid outlet hole 10 of the corresponding culture mechanism 4 are both located on the bottom wall of the slide groove 23; a baffle 24 is slidably installed in the slide groove 23 for closing the corresponding liquid inlet hole 9 and the liquid outlet hole 10 of the culture mechanism 4. In order to facilitate the installation of the baffle 24, the end of the slide groove 23 away from the center of the turntable 2 penetrates outwardly through the outer peripheral surface of the turntable 2, and an annular plate 15 is fixedly installed on the outer side of the turntable 2 by bolts to prevent the baffle 24 from escaping from the corresponding slide groove 23. The annular plate 15 blocks the end of the slide groove 23 away from the center of the turntable 2. When the baffle 24 is attached to the inner wall of the annular plate 15, the baffle 24 closes the top of the corresponding liquid inlet hole 9 and the liquid outlet hole 10, thereby preventing the cell culture fluid in the liquid inlet hole 9 or the liquid outlet hole 10 from being contaminated by the outside.

[0038] The turntable 2 is provided with a spring 25 for pushing the baffle 24 to fit on the annular plate 15. A slide bar 26 arranged parallel to the slide slot 23 is fixedly installed at one end of the baffle 24 near the center of the turntable 2. Two slide bars 26 are arranged side by side along the width direction of the baffle 24. A positioning hole 27 for inserting the slide bar 26 is provided at one end of the slide slot 23 near the center of the turntable 2. The spring 25 is located in the positioning hole 27. One end of the spring 25 abuts against the inner wall of the positioning hole 27, and the other end abuts against the end of the slide bar 26 away from the baffle 24. When there is no external force, the elastic force of the spring 25 pushes the baffle 24 to slide along the slide slot 23 in the direction away from the center of the turntable 2 and fits against the inner wall of the annular plate 15, so that the liquid inlet 9 and the liquid outlet 10 remain in a normally closed state.

[0039] A protruding plate 28 extending in a direction away from the axis of the turntable 2 is integrally formed on the baffle 24, and a notch 29 is provided on the annular plate 15 for the protruding plate 28 to pass through; a push plate 30 is provided on the front side of the lower end of the stand 14 and is driven by the second telescopic component and moves back and forth along the radial direction of the turntable 2, and the protruding plate 28 and the push plate 30 are located at the same height. Two guide rods 31 arranged in parallel on the left and right are fixedly installed on the rear side of the push plate 30 by bolts. The guide rods 31 extend forward and backward and are slidably installed on the stand 14 forward and backward to provide support and guidance for the forward and backward movement of the push plate 30; the second telescopic component is specifically a second electric push rod 32. The main body of the second electric push rod 32 is fixedly installed on the rear side of the stand 14 by bolts. The push rod of the second electric push rod 32 passes through the stand 14 forward and is fixedly connected to the push plate 30 by bolts. When the convex plate 28 located on the rear side of the turntable 2 rotates to the front of the push plate 30, the push plate 30 is driven forward by the extension of the second electric push rod 32, and the convex plate 28 and the corresponding baffle 24 are pushed by the push plate 30 to move in the direction close to the center of the turntable 2, so that the liquid inlet 9 and the liquid outlet 10 are opened.

[0040] like Figure 1 and Figure 6 As shown, an infrared transmitter 33 is fixedly installed on the front side of the lower end of the stand 14, and an infrared receiver 34 matching the infrared transmitter 33 is fixedly installed on the outer peripheral surface of the annular plate 15. The infrared receiver 34 corresponds to the culture mechanism 4 one by one. The infrared transmitter 33, the infrared receiver 34 and the motor 3 are all connected to the controller. When the infrared receiver 34 is aligned with the infrared transmitter 33, the liquid inlet hole 9 of the corresponding culture mechanism 4 rotates to the bottom of the injection tube 21, the corresponding liquid outlet hole 10 rotates to the bottom of the liquid extraction tube 22, and the corresponding convex plate 28 is aligned with the push plate 30, so as to accurately control the angle of rotation of the turntable 2 driven by the motor 3.

[0041] A cover plate 35 is provided on the top of the turntable 2 to cover the plurality of culture tanks 5 , thereby preventing the culture tanks 5 from being contaminated by the outside world.

[0042] like Figure 7 As shown, an organoid culture method is beneficial to an organoid culture device, the method comprising the following steps:

[0043] S1, sterilizing and disinfecting the above-mentioned organoid culture device;

[0044] S2, placing the organoid carrier 6 inoculated with the organoid in the culture tank 5;

[0045] S3, adding cell culture fluid into the culture tank 5;

[0046] S4, when the cell culture fluid in the culture tank 5 needs to be replaced, the motor 3 drives the turntable 2 to rotate, and the turntable 2 drives a group of culture mechanisms 4 to rotate backward to the liquid replacement mechanism 13, until the infrared receiver 34 corresponding to the culture mechanism 4 rotates to align with the infrared transmitter 33, and the motor 3 stops rotating; first, the second electric push rod 32 extends to drive the push plate 30 to move forward, and the push plate 30 pushes the baffle 24 to move toward the center of the turntable 2, so that the liquid inlet 9 and the liquid outlet 10 are opened; then the first electric push rod 19 extends to drive the mounting seat 1 6 moves downward, the mounting seat 16 drives the lower end of the injection tube 21 to be inserted into the liquid inlet hole 9, and the lower end of the extraction tube 22 to be inserted into the liquid outlet hole 10; then, new cell culture fluid is injected into the culture tank 5 through the injection tube 21, and the new cell culture fluid enters the culture tank 5 through the injection tube 21, the liquid inlet hole 9 and the liquid inlet channel 11 in sequence. At the same time, the old cell culture fluid in the culture tank 5 is extracted through the extraction tube 22, and the old cell culture fluid is sucked into the extraction tube 22 through the culture tank 5, the liquid outlet channel 12 and the liquid outlet hole 10 in sequence, thereby realizing the dynamic replacement of the cell culture fluid in the culture tank 5.

[0047] After the cell culture fluid in a group of culture mechanisms 4 is replaced, the first electric push rod 19 is contracted to drive the mounting seat 16 upward to return to its original position, and the mounting seat 16 drives the injection tube 21 to separate from the liquid inlet hole 9, and drives the liquid extraction tube 22 to separate from the liquid outlet hole 10; then the second electric push rod 32 is contracted to drive the push plate 30 to return to its original position backward, and the spring 25 pushes the baffle 24 to re-close the liquid inlet hole 9 and the liquid outlet hole 10. After the mounting seat 16 and the push plate 30 are returned to their original positions, the motor 3 can drive the turntable 2 to rotate a certain angle again, so that the next group of culture mechanisms 4 rotate to the liquid replacement mechanism 13, and then repeat the above steps to replace the cell culture fluid of the next group of culture mechanisms 4.

[0048] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. An organoid culture device, characterized in that: The invention comprises a base, on which a turntable driven by a power device and rotating around its own axis is provided, on the top of the turntable are provided a plurality of groups of culture mechanisms evenly spaced around the axis, the culture mechanisms comprising a culture tank located at the top of the turntable, in which an organoid carrying device is provided, and on the top of the turntable are provided a liquid inlet and a liquid outlet, in which a liquid inlet channel connecting the liquid inlet and the culture tank is provided, and in which a liquid outlet channel connecting the liquid outlet and the culture tank is provided; on the base located on the side of the turntable is provided a liquid changing mechanism, the liquid changing mechanism comprises a stand fixedly mounted on the base, on which a mounting seat driven to rise and fall by a first telescopic component is provided, on which a liquid injection tube matching the liquid inlet is fixedly mounted, and on which a liquid extraction tube matching the liquid outlet is also fixedly mounted; when the liquid inlet of one group of the culture mechanisms rotates to directly below the liquid injection tube, the corresponding liquid outlet rotates to directly below the liquid extraction tube.

2. The organoid culture device according to claim 1, characterized in that: The top of the turntable is provided with a slide groove extending radially thereof, and the slide groove corresponds to the culture mechanism one by one. A baffle is slidably installed in the slide groove to close the corresponding liquid inlet and liquid outlet of the culture mechanism. An annular plate is fixedly installed on the outer side of the turntable to prevent the baffle from escaping from the corresponding slide groove. When the baffle is in contact with the inner wall of the annular plate, the baffle closes the corresponding liquid inlet and liquid outlet. The turntable is provided with a spring for pushing the baffle to fit onto the annular plate.

3. The organoid culture device according to claim 2, characterized in that: The baffle is provided with a convex plate extending in a direction away from the axis of the turntable, and the annular plate is provided with a notch for the convex plate to pass through; the stand is also provided with a push plate driven by a second telescopic component and moving radially along the turntable, and the convex plate and the push plate are located at the same height.

4. The organoid culture device according to claim 2, characterized in that: An infrared transmitter is fixedly mounted on the stand, an infrared receiver matching the infrared transmitter is fixedly mounted on the annular plate, the infrared receiver corresponds to the culture mechanism one by one, and when the infrared receiver is aligned with the infrared transmitter, the corresponding liquid inlet of the culture mechanism rotates to be directly below the injection tube, the corresponding liquid outlet rotates to be directly below the extraction tube, and the corresponding convex plate is aligned with the push plate.

5. The organoid culture device according to claim 2, characterized in that: A sliding rod parallel to the sliding groove is fixedly mounted on the baffle, and a positioning hole for inserting the sliding rod is provided at one end of the sliding groove close to the center of the turntable, one end of the spring abuts against the inner wall of the positioning hole, and the other end abuts against the end of the sliding rod away from the baffle.

6. The organoid culture device according to claim 1, characterized in that: A vertically extending slide rail is fixedly mounted on the stand, and a sliding block matching the slide rail is fixedly mounted on the mounting seat.

7. The organoid culture device according to claim 1, characterized in that: A cover plate covering the plurality of culture tanks is provided on the top of the turntable.

8. The organoid culture device according to claim 1, characterized in that: The liquid inlet channel and the liquid outlet channel are respectively connected to two opposite sides of the culture tank.

9. An organoid culture method using the organoid culture device according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: S1, sterilizing and disinfecting the organoid culture device according to any one of claims 1 to 8; S2, placing the organoid carrier device inoculated with the organoid in the culture tank; S3, adding cell culture fluid into the culture tank; S4, when the cell culture fluid in the culture tank needs to be replaced, the culture mechanism is driven by the turntable to rotate to the fluid replacement mechanism, and the injection tube and the extraction tube are driven by the mounting seat to be inserted into the liquid inlet and the liquid outlet respectively, and new cell culture fluid is injected into the culture tank through the injection tube, and the old cell culture fluid in the culture tank is extracted through the extraction tube.

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