Circular polarization fluorescence spectrophotometer equipped with light collector and control method of circular polarization fluorescence spectrophotometer

Abstract

The invention discloses a circular polarization fluorescence spectrometer equipped with a light concentrator and a control method thereof, and relates to the technical field of spectrometers, the light concentrator is ellipsoid-shaped, a first focus of the light concentrator is used for placing a sample, a circular polarization light conversion assembly is arranged on an output light path of the light concentrator, and a second focus of the light concentrator is used for placing a sample. The circularly polarized light conversion assembly is used for adjusting a fluorescence spectrum signal, the light splitting detection system is used for acquiring the fluorescence spectrum signal, and the light concentrator is arranged, so that the circularly polarized fluorescence spectrometer with the light concentrator has the advantages of high test speed, high signal-to-noise ratio and high sample resolution.

Description

Technical Field

[0001] The present application relates to the technical field of spectrometers, and in particular to a circular polarization fluorescence spectrometer equipped with a light concentrator and a control method thereof. Background Art

[0002] Chiral luminescent materials have unique optical properties, which are professionally called circularly polarized luminescence. This optical activity plays an important role in modern science and technology. Polarization is one of the inherent important properties of light. Circularly polarized light carries rich optical information and has been widely used in many fields such as quantum computing, information encryption, three-dimensional (3D) display and light-emitting devices.

[0003] Circular polarization fluorescence spectrometer (CPL) has a wide range of uses and application prospects in the fields of chiral luminescent materials, fluorescent proteins, information display and storage, chiral electronics, etc., which has attracted great attention and interest from scientists. Circular polarization fluorescence spectrometer can provide structural information of molecular excited states and characterize chiral asymmetric structures, becoming an important means to study the stereo configuration of excited state chiral organic compounds. Existing circular polarization fluorescence spectrometers have the disadvantages of slow test speed, low signal-to-noise ratio and unsatisfactory sample resolution. Summary of the invention

[0004] The purpose of the present application is to provide a circular polarization fluorescence spectrometer equipped with a light concentrator and a control method thereof, which has the advantages of fast testing speed, high signal-to-noise ratio and high sample resolution.

[0005] To achieve the above objectives, this application provides the following solutions: In a first aspect, the present application provides a circular polarization fluorescence spectrometer equipped with a light concentrator, comprising: Light concentrator, circularly polarized light conversion assembly and spectroscopic detection system; The light concentrator is in the shape of an ellipsoid; The first focus of the light concentrator is used to place the sample; The circularly polarized light conversion component is arranged on the output light path of the light concentrator; The circularly polarized light conversion component is used to adjust the fluorescence spectrum signal; The spectroscopic detection system is used to obtain fluorescence spectrum signals.

[0006] Optionally, the circular polarization fluorescence spectrometer equipped with a light concentrator further comprises: Excitation light source; the excitation light source is used to irradiate the sample.

[0007] Optionally, the excitation light source is a laser lamp, a xenon lamp or an LED lamp.

[0008] Optionally, the circularly polarized light conversion assembly comprises: a first convex lens, a circularly polarized light converter, a linear polarizer and a second convex lens; The first convex lens is arranged on the output light path of the light concentrator; the distance from the second focus of the light concentrator to the first convex lens is equal to the focal length of the first convex lens; The circularly polarized light converter is arranged on the output light path of the first convex lens; The linear polarizer is arranged on the output light path of the circularly polarized light converter; The second convex lens is arranged on the output light path of the linear polarizer; The second convex lens is arranged on the input optical path of the spectroscopic detection system; When the circularly polarized light converter is at a forward voltage, the spectroscopic detection system acquires the intensity of the right-handed circularly polarized light signal; When the circularly polarized light converter is at a negative voltage, the spectroscopic detection system acquires the signal intensity of the left-handed circularly polarized light.

[0009] Optionally, the spectroscopic detection system comprises: a first spectroscopic slit, a first concave reflector, a first polarizing prism, a second concave reflector, a second spectroscopic slit, a third concave reflector, a second polarizing prism, a fourth concave reflector and a photomultiplier tube; The first beam splitting slit is arranged on the output light path of the second convex lens; The first concave reflector is arranged on the output light path of the first beam splitting slit; The first polarizing prism is arranged on the output light path of the first concave reflector; The second concave reflector is arranged on the output light path of the first polarizing prism; The second beam splitting slit is arranged on the output light path of the second concave reflecting mirror; The third concave reflecting mirror is arranged on the output light path of the second beam splitting slit; The second polarizing prism is arranged on the output light path of the third concave reflector; The fourth concave reflector is arranged on the output light path of the second polarizing prism; The photomultiplier tube is arranged on the output light path of the fourth concave reflecting mirror.

[0010] Optionally, the circular polarization fluorescence spectrometer equipped with a light concentrator further comprises: a calculation module; The computing module is connected to the spectroscopic detection system; The spectroscopic detection system is used to determine the sample test result according to the fluorescence spectrum signal; the fluorescence spectrum signal includes the intensity of the right-handed circularly polarized light signal and the intensity of the left-handed circularly polarized light signal.

[0011] In a second aspect, the present application provides a control method for a circularly polarized fluorescence spectrometer equipped with a light concentrator, the control method for a circularly polarized fluorescence spectrometer equipped with a light concentrator is applied to the circularly polarized fluorescence spectrometer equipped with a light concentrator, and the control method for a circularly polarized fluorescence spectrometer equipped with a light concentrator comprises: Control the circularly polarized light converter to be in a forward voltage to obtain the intensity of the right-handed circularly polarized light signal; Control the circularly polarized light converter to be in a negative voltage to obtain the signal intensity of the left-handed circularly polarized light; The sample test result is determined according to the right-handed circularly polarized light signal intensity and the left-handed circularly polarized light signal intensity; the sample test result includes CPL data, CPL asymmetry factor and CPL fluorescence intensity.

[0012] Optionally, the CPL data is: ; in, For CPL data, is the signal intensity of left-handed circularly polarized light, is the signal intensity of right-hand circularly polarized light.

[0013] Optionally, the CPL asymmetry factor is: ; in, is the CPL asymmetry factor.

[0014] Optionally, the CPL fluorescence intensity is the sum of the right-handed circularly polarized light signal intensity and the left-handed circularly polarized light signal intensity.

[0015] According to the specific embodiments provided in this application, this application discloses the following technical effects: The present application provides a circularly polarized fluorescence spectrometer equipped with a light concentrator and a control method thereof. The light concentrator is in the shape of an ellipsoid. The first focus of the light concentrator is used to place a sample. A circularly polarized light conversion component is arranged on the output light path of the light concentrator. The circularly polarized light conversion component is used to adjust the fluorescence spectrum signal. The spectroscopic detection system is used to obtain the fluorescence spectrum signal. By setting the light concentrator, the circularly polarized fluorescence spectrometer equipped with the light concentrator has the advantages of fast testing speed, high signal-to-noise ratio and high sample resolution. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 This is a configuration diagram of a circular polarization fluorescence spectrometer system configured with a light concentrator in one embodiment of the present application.

[0018] Figure 2 This is the system configuration diagram of the existing commercial circular polarization fluorescence spectrometer CPL-300.

[0019] Figure 3 This is a circularly polarized fluorescence spectrum of (S)-2,2'-bis((4-(9,9-dimethylacridinyl-10(9H))phenyl)sulfonyl)-1,1'-binaphthyl tested by a commercial circularly polarized fluorescence spectrometer CPL-300 in one embodiment of the present application.

[0020] Figure 4 This is a circularly polarized fluorescence spectrum of (R)-2,2'-bis((4-(9,9-dimethylacridinyl-10(9H))phenyl)sulfonyl)-1,1'-binaphthyl tested by a commercial circularly polarized fluorescence spectrometer CPL-300 in one embodiment of the present application.

[0021] Figure 5 This is a circularly polarized fluorescence spectrum of (S)-2,2'-bis((4-(9,9-dimethylacridinyl-10(9H))phenyl)sulfonyl)-1,1'-binaphthyl measured by a circularly polarized fluorescence spectrometer for a light concentrator in one embodiment of the present application.

[0022] Figure 6 This is a circularly polarized fluorescence spectrum of (R)-2,2'-bis((4-(9,9-dimethylacridinyl-10(9H))phenyl)sulfonyl)-1,1'-binaphthyl measured by a circularly polarized fluorescence spectrometer for a light concentrator in one embodiment of the present application.

[0023] Figure 7 This is a schematic diagram of the size of the light concentrator model in one embodiment of the present application. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0025] In order to make the above-mentioned objects, features and advantages of the present application more obvious and easy to understand, the present application is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0026] In an exemplary embodiment, Figure 1 As shown, a circular polarization fluorescence spectrometer equipped with a light concentrator is provided, comprising: a light concentrator, a circular polarization light conversion component and a spectroscopic detection system. The light concentrator is in the shape of an ellipsoid. The first focus of the light concentrator is used to place a sample. The circular polarization light conversion component is arranged on the output light path of the light concentrator. The circular polarization light conversion component is used to adjust the fluorescence spectrum signal. The spectroscopic detection system is used to obtain the fluorescence spectrum signal.

[0027] The circular polarization fluorescence spectrometer equipped with a light concentrator also includes an excitation light source. The excitation light source is used to irradiate the sample. The excitation light source is a laser light, a xenon lamp or an LED lamp.

[0028] The circularly polarized light conversion component includes: a first convex lens, a circularly polarized light converter, a linear polarizer and a second convex lens; the first convex lens is arranged on the output light path of the light concentrator; the distance from the second focus of the light concentrator to the first convex lens is equal to the focal length of the first convex lens; the circularly polarized light converter is arranged on the output light path of the first convex lens; the linear polarizer is arranged on the output light path of the circularly polarized light converter; the second convex lens is arranged on the output light path of the linear polarizer; the second convex lens is arranged on the input light path of the spectroscopic detection system; when the circularly polarized light converter is at a positive voltage, the spectroscopic detection system obtains the signal intensity of right-handed circularly polarized light; when the circularly polarized light converter is at a negative voltage, the spectroscopic detection system obtains the signal intensity of left-handed circularly polarized light.

[0029] The spectroscopic detection system includes: a first spectroscopic slit, a first concave reflector, a first polarization prism, a second concave reflector, a second spectroscopic slit, a third concave reflector, a second polarization prism, a fourth concave reflector and a photomultiplier tube; the first spectroscopic slit is arranged on the output light path of the second convex lens; the first concave reflector is arranged on the output light path of the first spectroscopic slit; the first polarization prism is arranged on the output light path of the first concave reflector; the second concave reflector is arranged on the output light path of the first polarization prism; the second spectroscopic slit is arranged on the output light path of the second concave reflector; the third concave reflector is arranged on the output light path of the second spectroscopic slit; the second polarization prism is arranged on the output light path of the third concave reflector; the fourth concave reflector is arranged on the output light path of the second polarization prism; and the photomultiplier tube is arranged on the output light path of the fourth concave reflector.

[0030] The circular polarization fluorescence spectrometer equipped with a light concentrator also includes: a calculation module. The calculation module is connected to a spectroscopic detection system. The spectroscopic detection system is used to determine the sample test result according to the fluorescence spectrum signal. The fluorescence spectrum signal includes the intensity of the right-handed circularly polarized light signal and the intensity of the left-handed circularly polarized light signal.

[0031] The sample is placed at one focus of the ellipsoid light concentrator, and the laser / xenon lamp / LED is used as the excitation light source to illuminate the sample. At this time, the emitted light converges at the other focus of the ellipsoid. A convex lens is placed at the focal length after the convergence focus to convert the divergent light into parallel light. Then, the right-handed circularly polarized light in the elliptically polarized light generated by the sample is converted into 0-degree linear polarized light through the circular polarization converter. After passing through the 0-degree polarizer and entering the spectroscopic detection system, the signal obtained is the signal intensity of the right-handed circularly polarized light (I R Then a negative voltage is applied to the circular polarization converter, and the signal obtained after passing through the 0-degree polarizer and entering the spectroscopic detection system is a left-handed circularly polarized light signal (I L The data processing system uses the sum of the right-handed and left-handed circularly polarized light spectral signals as the total fluorescence spectral signal, i.e. (I L +I R ), the difference between the right-handed and left-handed circularly polarized light spectral signals is taken as the circularly polarized fluorescence difference spectrum signal, that is, (I L -I R The final result of the computer test includes three parts: 1. CPL data, the value is θ=(I L -I R )×ln10×(180 / π) / 4, the unit is deg (degree) or mdeg (millidegree). 2. CPL asymmetry factor g=2×(I L -I R ) / (I L +I R ), no unit. 3. CPL fluorescence intensity (I L +I R ).

[0032] Figure 7 Point A is the sample placement point, AG is 44 mm long, the short axis (GF) is 23.75 mm long, and the long axis (EG) is 50 mm long. Figure 7 , the light concentrator is a self-designed ellipsoidal reflective mirror. The sample is placed at one focus of the ellipsoid structure, and the condenser is placed at a focal length (f) behind the other focus to receive the emitted light of the sample. The ellipsoid designed in this application has a long side of 100mm and a short side of 47.5mm. The sample is placed 44mm away from the center of the ellipsoid in the long side direction, and a lens with a focal length of 16mm is placed 16mm behind the other focus. The device can converge the divergent light to the spectroscopic detection system. This application protects the design principle and structure of the ellipsoid.

[0033] In a common circular polarization fluorescence spectrometer (CPL), after the sample is excited by the light source, since the fluorescence quantum yield (ratio of emitted light to incident light) of most samples is less than 10%, only 10% of the maximum effective light can enter the subsequent optical system. In addition, since the direction of the emitted light is disordered, only no more than 10% of the emitted light will enter the polarization spectrometer for spectroscopic analysis. At this time, the effective signal of the sample is less than 1% of the total incident light intensity (such as Figure 2 As shown), it can be seen that the signal-to-noise ratio of the circular polarization fluorescence spectrometer is much lower than that of the circular dichroism spectrometer. The quantum yield of the sample is an inherent property of the sample and cannot be changed. Therefore, the present application is committed to solving the problem of emitted light. The specific method is to place the sample at one focus of an elliptical light concentrator. After the excitation light source excites the sample, the emitted light must converge at another focus of the elliptical concentrator. In theory, more than 90% of the emitted light of the sample can enter the subsequent spectroscopic system. Therefore, the signal detected by the PMT is at least 9 times higher than the original instrument signal, and the signal-to-noise ratio of the instrument is greatly increased (such as Figure 1 As shown). The sample tested in this application is (R / S)-2,2'-bis((4-(9,9-dimethylacridinyl-10(9H))phenyl)sulfonyl)-1,1'-binaphthalene in the article "Chiral Sulfonyl Binaphthalene-Based Thermally Activated Delayed Fluorescence Materials for Circularly Polarized Electroluminescence" published in the internationally renowned journal Advanced Optical Materials (Adv. Optical Mater.) Issue 12, 2024, No. 2302730. Figure 3 and Figure 4 They are the test results of (R / S)-2,2'-bis((4-(9,9-dimethylacridinyl-10(9H))phenyl)sulfonyl)-1,1'-binaphthyl film on the most commonly used commercial circular polarization fluorescence spectrometer CPL-300. The test results have very low resolution. Figure 5 and Figure 6 They are the test results of (R / S)-2,2'-bis((4-(9,9-dimethylacridinyl-10(9H))phenyl)sulfonyl)-1,1'-binaphthyl film on a circular polarization fluorescence spectrometer equipped with a light concentrator. The signal-to-noise ratio of the test results has been greatly improved compared with the commercial CPL-300.

[0034] In an exemplary embodiment, a control method of a circular polarization fluorescence spectrometer equipped with a light concentrator is provided, and the control method of a circular polarization fluorescence spectrometer equipped with a light concentrator is applied to the circular polarization fluorescence spectrometer equipped with a light concentrator, and the control method of a circular polarization fluorescence spectrometer equipped with a light concentrator includes: Step 1: Control the circularly polarized light converter to be in a forward voltage to obtain the right-handed circularly polarized light signal intensity.

[0035] Step 2: Control the circularly polarized light converter to be at a negative voltage to obtain the left-handed circularly polarized light signal intensity.

[0036] Step 3: Determine the sample test result according to the right-handed circularly polarized light signal intensity and the left-handed circularly polarized light signal intensity. The sample test result includes CPL data, CPL asymmetry factor and CPL fluorescence intensity.

[0037] The CPL data is: .

[0038] in, For CPL data, is the signal intensity of left-handed circularly polarized light, is the signal intensity of right-hand circularly polarized light.

[0039] The CPL asymmetry factor is: .

[0040] in, is the CPL asymmetry factor.

[0041] The CPL fluorescence intensity is the sum of the right-handed circularly polarized light signal intensity and the left-handed circularly polarized light signal intensity.

[0042] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0043] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and core ideas of this application. At the same time, for those skilled in the art, according to the ideas of this application, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting this application.

Claims

1. A circular polarization fluorescence spectrometer equipped with a light concentrator, characterized in that: include: Light concentrator, circularly polarized light conversion assembly and spectroscopic detection system; The light concentrator is in the shape of an ellipsoid; The first focus of the light concentrator is used to place the sample; The circularly polarized light conversion component is arranged on the output light path of the light concentrator; The circularly polarized light conversion component is used to adjust the fluorescence spectrum signal; The spectroscopic detection system is used to obtain fluorescence spectrum signals.

2. The circular polarization fluorescence spectrometer equipped with a light concentrator according to claim 1, characterized in that: The circular polarization fluorescence spectrometer equipped with a light concentrator also includes: Excitation light source; the excitation light source is used to irradiate the sample.

3. The circular polarization fluorescence spectrometer equipped with a light concentrator according to claim 2, characterized in that: The excitation light source is a laser lamp, a xenon lamp or an LED lamp.

4. The circular polarization fluorescence spectrometer equipped with a light concentrator according to claim 1, characterized in that: The circularly polarized light conversion assembly comprises: a first convex lens, a circularly polarized light converter, a linear polarizer, and a second convex lens; The first convex lens is arranged on the output light path of the light concentrator; the distance from the second focus of the light concentrator to the first convex lens is equal to the focal length of the first convex lens; The circularly polarized light converter is arranged on the output light path of the first convex lens; The linear polarizer is arranged on the output light path of the circularly polarized light converter; The second convex lens is arranged on the output light path of the linear polarizer; The second convex lens is arranged on the input optical path of the spectroscopic detection system; When the circularly polarized light converter is at a forward voltage, the spectroscopic detection system acquires the intensity of the right-handed circularly polarized light signal; When the circularly polarized light converter is at a negative voltage, the spectroscopic detection system acquires the signal intensity of the left-handed circularly polarized light.

5. The circular polarization fluorescence spectrometer equipped with a light concentrator according to claim 4, characterized in that: The spectroscopic detection system comprises: a first spectroscopic slit, a first concave reflector, a first polarizing prism, a second concave reflector, a second spectroscopic slit, a third concave reflector, a second polarizing prism, a fourth concave reflector and a photomultiplier tube; The first beam splitting slit is arranged on the output light path of the second convex lens; The first concave reflector is arranged on the output light path of the first beam splitting slit; The first polarizing prism is arranged on the output light path of the first concave reflector; The second concave reflector is arranged on the output light path of the first polarizing prism; The second beam splitting slit is arranged on the output light path of the second concave reflecting mirror; The third concave reflecting mirror is arranged on the output light path of the second beam splitting slit; The second polarizing prism is arranged on the output light path of the third concave reflector; The fourth concave reflector is arranged on the output light path of the second polarizing prism; The photomultiplier tube is arranged on the output light path of the fourth concave reflecting mirror.

6. The circular polarization fluorescence spectrometer equipped with a light concentrator according to claim 4, characterized in that: The circular polarization fluorescence spectrometer equipped with a light concentrator further comprises: a calculation module; The computing module is connected to the spectroscopic detection system; The spectroscopic detection system is used to determine the sample test result according to the fluorescence spectrum signal; the fluorescence spectrum signal includes the intensity of the right-handed circularly polarized light signal and the intensity of the left-handed circularly polarized light signal.

7. A control method for a circular polarization fluorescence spectrometer equipped with a light concentrator, characterized in that: The control method of the circular polarization fluorescence spectrometer equipped with a light concentrator is applied to the circular polarization fluorescence spectrometer equipped with a light concentrator as claimed in any one of claims 1 to 6, and the control method of the circular polarization fluorescence spectrometer equipped with a light concentrator comprises: Control the circularly polarized light converter to be in a forward voltage to obtain the intensity of the right-handed circularly polarized light signal; Control the circularly polarized light converter to be in a negative voltage to obtain the signal intensity of the left-handed circularly polarized light; The sample test result is determined according to the right-handed circularly polarized light signal intensity and the left-handed circularly polarized light signal intensity; the sample test result includes CPL data, CPL asymmetry factor and CPL fluorescence intensity.

8. The control method of the circular polarization fluorescence spectrometer equipped with a light concentrator according to claim 7, characterized in that: The CPL data is: ； in, For CPL data, is the signal intensity of left-handed circularly polarized light, is the signal intensity of right-hand circularly polarized light.

9. The control method of the circular polarization fluorescence spectrometer equipped with a light concentrator according to claim 8, characterized in that: The CPL asymmetry factor is: ； in, is the CPL asymmetry factor.

10. The control method of the circular polarization fluorescence spectrometer equipped with a light concentrator according to claim 8, characterized in that: The CPL fluorescence intensity is the sum of the right-handed circularly polarized light signal intensity and the left-handed circularly polarized light signal intensity.

Images