Pesticide composition containing sulfur-fluorine oxime ether

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing sulfur-fluorine oxime ether. According to the pesticide composition containing the sulfur-fluorine oxime ether, the pesticide composition comprises an effective component, and the effective component is formed by compounding the sulfur-fluorine oxime ether and the doramectin, or is formed by compounding the sulfur-fluorine oxime ether and the ivermectin. The pesticide composition disclosed by the invention has a very good synergistic effect on plant pests, and the control effect on a target can be improved; compared with a single agent, the pesticide composition provided by the invention can reduce the application amount and the application frequency of the agent, and reduce the prevention and treatment cost, the pesticide residue and the environmental pressure; compared with a single agent, the pesticide composition provided by the invention can reduce the risk of drug resistance, delay the generation and accumulation of drug resistance of target pests, and prolong the service life of the pesticide.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular relates to a pesticide composition containing sulfur-containing fluorooxime ether. Background Art

[0002] Thiofuroxime is a non-ester pyrethroid insecticide with independent intellectual property rights designed and synthesized by Hunan Chemical Research Institute. CAS: 860028-12-2, molecular formula: C 23 H 21 ClFNOS, relative molecular weight: 413.935, structural formula is as follows:

[0003]

[0004] Thiofuroxime has a broad insecticide spectrum and rapid action, and has good insecticidal activity against multiple pests including cabbage loopers, aphids, leafhoppers and tea loopers. Thiofuroxime is an environmentally friendly compound with low toxicity, low residue and safety to non-target organisms.

[0005] Doramectin is a macrolide antibiotic produced by fermentation of a genetically recombinant new strain of Streptomyces avermitilis using cyclohexanecarboxylic acid as a precursor. It belongs to the avermectin class of biological pesticides. The mechanism of action of doramectin is to interfere with neurophysiological activities and stimulate the release of γ-aminobutyric acid. The increase in the release of γ-aminobutyric acid increases the normal resting potential of the post-synaptic cells, making it difficult for the nerves to transmit the stimulus to the muscles, which in turn makes the muscles unable to contract, resulting in paralysis of the insect body and its expulsion. Doramectin has contact and stomach poisoning effects on a variety of insects, mites and nematodes.

[0006] Ivermectin is a semi-synthetic macrolide agricultural antibiotic insecticide, which is a product of selective hydrogenation reduction of the 22 and 23 double bonds with avermectin as the lead compound. Ivermectin can interfere with the neurophysiological activities of insects and mites, stimulate the release of the neurotransmitter γ-aminobutyric acid (GABA), block the nerve conduction between nerve endings and muscle cells, and cause paralysis symptoms in the target, making it unable to move and feed, and eventually die. Ivermectin has stomach poison and contact killing effects, but cannot kill eggs.

[0007] In agricultural production, long-term use of a single pesticide or unscientific use of pesticides will lead to a gradual increase in the resistance of target pests and diseases, while excessive use of pesticides will not only lead to environmental pollution and increased production costs, but also aggravate the development of resistance, which is not conducive to the sustainable development of agriculture. The inventors found through indoor biological activity tests that when thiofluoxetine is compounded with doramectin or ivermectin, within a certain range, it exhibits a synergistic effect on plant pests, which can improve the control effect, reduce the amount of medicine used, and reduce production costs compared with a single agent. Summary of the invention

[0008] Based on the above situation, the purpose of the present invention is to provide a pesticide composition containing sulfur fluoxime ether, which can achieve synergistic effect through compounding, improve the prevention and control effect, reduce the amount of medicine used, reduce production costs, and effectively overcome the problems existing in the use of a single pesticide agent.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A pesticide composition containing thiofluoxetine, comprising an active ingredient, wherein the active ingredient is compounded by thiofluoxetine and doramectin, or by thiofluoxetine and ivermectin.

[0011] Furthermore, the mass ratio of thiofluanidazole to doramectin is 1-12:16-1.

[0012] Furthermore, the mass ratio of thiofluanidazole to ivermectin is 1-20:3-1.

[0013] Furthermore, the total mass of the pesticide composition is 100%, and the mass of the active ingredient accounts for 1-90% of the total mass of the pesticide composition.

[0014] Furthermore, the mass of the active ingredient accounts for 2-70% of the total mass of the pesticide composition.

[0015] Furthermore, the pesticide composition also includes auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, disintegrants, inert fillers, solvents, defoamers, synergists, preservatives and thickeners.

[0016] Furthermore, the pesticide composition can be prepared into a variety of formulations permitted in agriculture.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] (1) The pesticide composition of the present invention has a good synergistic effect on plant pests and can improve the control effect on the target.

[0019] (2) Compared with a single agent, the pesticide composition of the present invention can reduce the amount and frequency of application of the agent, thereby reducing the cost of prevention and control, pesticide residues and environmental pressure.

[0020] (3) Compared with a single agent, the pesticide composition of the present invention can reduce the risk of drug resistance, delay the generation and accumulation of drug resistance in target pests, and extend the service life of the agent. DETAILED DESCRIPTION

[0021] In order to make the technical scheme, purpose and advantages of the present invention more clearly understood, the present invention is described by the following examples, but the present invention can be implemented in various forms and should not be limited by the examples described herein. The present invention has been described below with general descriptions and specific implementation schemes, on which basis, some modifications or improvements can be made, which is obvious to those skilled in the art, and all equivalent changes made on the basis of the technical scheme of this application fall within the protection scope of the present invention.

[0022] 1. Indoor biological activity test (refer to "NY / T 1154.1-2006 Pesticide Indoor Bioassay Test Guidelines Insecticides Part 1: Contact Activity Test Spot Method")

[0023] 1.1 Test pests

[0024] Brown planthoppers (Nilaparvata lugens) were collected from rice fields and reared with fresh and clean rice seedlings for four generations. The third instar nymphs of brown planthoppers with uniform age and strong vitality were selected as test objects.

[0025] 1.2 Test agents

[0026] 95% thiofluanidazole technical, 97% doramectin technical, 95% ivermectin technical

[0027] 1.3 Test methods

[0028] After dissolving the test agent with dimethyl sulfoxide, dilute it with 0.1% Tween-80 aqueous solution to form a single-dose stock solution, then set up multiple groups of ratios, and then dilute the single-dose stock solution and each ratio mixture with 0.1% Tween-80 aqueous solution to 5 mass concentration gradients for use.

[0029] The test insects were anesthetized with CO2 for 20 seconds to make them unconscious, and 0.5 μL of the drug solution was dripped onto the pronotum of the nymphs. After dripping, the test insects were transferred to transparent plastic boxes (15 cm long, 10 cm wide, and 8 cm high). Each plastic box was inoculated with 20 test insects and 10 rice seedlings at the 3-leaf stage (the roots of the seedlings were moisturized with absorbent cotton), and the box lid was closed (air holes were pierced on the box lid). The insects were transferred to a temperature of 25±1°C, a relative humidity of about 750±5%, and a photoperiod of 14L / 10D for feeding and observation. Each plastic box was repeated 1 time, and each concentration was repeated 4 times. The treatment of dripping an equal amount of 0.1% Tween-80 aqueous solution was used as a blank control.

[0030] The mortality of the test insects was observed for 24 h (the test insects were considered dead if they could not turn over within 30 s), and the mortality rate and corrected mortality rate of the nymphs in each treatment were calculated.

[0031]

[0032] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0033]

[0034] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0035] 1.4 Data Analysis

[0036] DPS software was used to perform regression analysis on the logarithmic values ​​of the concentration of each treatment agent and the corrected mortality probability value of each treatment to calculate the LC of each treatment agent. 50 , and the co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method.

[0037]

[0038] In the above formula: ATI--the toxicity index of the mixture; S--the LC of the standard agent 50 , the unit is mg / L; M--LC of the mixture 50 , unit is mg / L.

[0039] TTI=TI A ×P A +TI B ×P B

[0040] In the above formula: TTI-theoretical toxicity index of the mixture; TI A --Toxicity index of A agent; P A --The percentage of agent A in the mixture, in percentage (%); TI B --Toxicity index of agent B; P B --The percentage of agent B in the mixture, in percentage (%).

[0041]

[0042] In the above formula: CTC--co-toxicity coefficient; ATI--actual toxicity index of the mixture; TTI--theoretical toxicity index of the mixture.

[0043] 1.5 Efficacy evaluation

[0044] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0045] Table 1 Results of indoor bioactivity test of thiofluanid and doramectin against rice brown planthopper

[0046] Drug name and ratio LC50(mg / L) ATI TTI CTC Thiofuroxime 16.6481 100.0000 -- -- Doramectin 9.4072 176.9719 -- -- Thioflurane 1: Doramectin 16 7.6712 217.0208 172.4441 125.8499 Thioflurane 1: Doramectin 8 5.1581 322.7564 168.4195 191.6384 Thioflurane 1: Doramectin 4 7.3926 225.1995 161.5775 139.3755 Thioflurane 1: Doramectin 1 6.7729 245.8046 138.4859 177.4943 Thiofent 4: Doramectin 1 11.0755 150.3147 115.3944 130.2617 Thiofent 8: Doramectin 1 12.5142 133.0337 108.5524 122.5525 Thiofent 12: Doramectin 1 9.9638 167.0859 105.9209 157.7459

[0047] It can be seen from the test results in Table 1 that when the mass ratio of thiofluanidine to doramectin is in the range of 1-12:16-1, the co-toxicity coefficient is greater than 120, and the combined effect shows a synergistic effect.

[0048] Table 2 Indoor bioactivity test results of thiofluanid and ivermectin against rice brown planthopper

[0049] Drug name and ratio LC50(mg / L) ATI TTI CTC Thiofluoxime 16.6481 100.0000 -- -- Ivermectin 5.1195 325.1900 -- -- Thiofent 1: Ivermectin 3 3.4393 484.0549 268.8925 180.0180 Thiofent 1: Ivermectin 1 5.6057 296.9852 212.5950 139.6953 Thiofent 3: Ivermectin 1 8.6552 192.3480 156.2975 123.0653 Thioflavone 5: Ivermectin 1 7.1725 232.1101 137.5317 168.7685 Thioflavone 10: Ivermectin 1 10.7608 154.7106 120.4718 128.4206 Thioflavone 20: Ivermectin 1 9.2879 179.2450 110.7233 161.8855

[0050] It can be seen from the test results in Table 2 that when the mass ratio of thiofluanidine to ivermectin is in the range of 1-20:3-1, the co-toxicity coefficient is greater than 120, and the combined effect shows a synergistic effect.

[0051] In summary, through indoor biological activity tests, it was found that when thiofluanidine was combined with doramectin or ivermectin, it showed a synergistic effect on plant pests within a certain range. Compared with a single agent, it can improve the control effect, reduce the amount of medicine used, and reduce production costs.

Claims

1. A pesticide composition containing sulfur fluoxime ether, characterized in that: The pesticide composition comprises an active ingredient, and the active ingredient is compounded by thiofluanid and doramectin, or compounded by thiofluanid and ivermectin.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of thiofluanidazole to doramectin is 1-12:16-1.

3. The pesticide composition according to claim 1, characterized in that The mass ratio of thiofluanidazole to ivermectin is 1-20:3-1.

4. The pesticide composition according to claim 1, characterized in that The total mass of the pesticide composition is 100%, and the mass of the active ingredient accounts for 1-90% of the total mass of the pesticide composition.

5. The pesticide composition according to claim 4, characterized in that The mass of the active ingredient accounts for 2-70% of the total mass of the pesticide composition.

6. The pesticide composition according to claim 1, characterized in that The pesticide composition further comprises auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, disintegrants, inert fillers, solvents, defoamers, synergists, preservatives and thickeners.

7. The pesticide composition according to claim 1, characterized in that The pesticide composition can be prepared into various formulations permitted in agriculture.

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